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Article
Inhibitory effect of honey on some bacterial infections

Author: Najla'a Nabhan Yassein
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2011 Volume: 7 Issue: 3 Pages: 227-241
Publisher: Diyala University جامعة ديالى

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Abstract

The antibacterial profiles of honey were examined against clinical isolates of
Staphyllococcus aureus and Pseudomonas aeruginosa. Agar well diffusion method was used
in the antibacterial susceptibility studies. This study revealed that the honey obtained from
Agricultural college of Baghdad University was effective against bacteria.The highest
inhibitory zone of S. aureus reached to 20mm in diameter at 200 mg/ml of honey
concentration but not affected on P. aeruginosa. Wounds were done in male mice by using
shaver and then infected with 0.1ml of both bacterial suspensions with concentration 108
CFU/ml.Symptomes of infections were appeared after 24-48 hours. Three concentrations of
honey were used (100,200 and 300) mg/ml for treatment of skin infections by using 0.1 ml of
honey,also gentamycin ointment and normal saline were used. Symptomes of recovery based
on wound healing were noticed after 10,6,9,11 and 15 days for S. aureus and 12,8,13,15 and
18 days for P. aeruginosa when treated with 100,200 and 300mg/ml of honey,gentamycin and
normal saline respectively. 200 mg/ml of honey concentration showed the best concentration
for skin infection treatment. This study,therefore, suggest that honey could have strong
biocidal effect against S. aureus (both in vitro and in vivo) and against P. aeruginosa (in
vivo),therefore,have the potential effective role in the treatment of skin infection.


Article
STUDY THE EFFECT OF PURIFIED CATECHINS OF GREEN TEA ON THE MOST IMPORTANT CAUSES OF BACTERIAL INFECTIONS OF THE URINARY TRACT AND SYNERGISTIC EFFECT
دراسة تأثير الكاتشين المنقى من الشاي الاخضر في أهم المسببات البكتيرية لالتهابات المجاري البولية والتأثير التآزري عليها

Authors: ESSAM F.A.Al-jumaily عصام فاضل الجميلي --- Yasir A.J.Al-Abdli --- Nazar E.Nasser ياسر عادل جبار --- نزار ادور ناصر
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2011 Volume: 52 Issue: 3 Pages: 324-328
Publisher: Baghdad University جامعة بغداد

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Abstract

Two hundred urine samples were collected from patients infected with acute and chronic urinary tract infection, 85 bacterial isolate were isolated from these samples and were diagnosed using biochemical tests and kit API test, most of them identified E.coli (23.5%) K.pneumoniae rate (17.6%). Isolates were selected E.coli and K.pneumoniae according to their isolation rate from urinary tract infection cases and their antibiotic resistance. The inhibition effect of the purified catechin from the green tea was studied when the isolates were grown in the following concentrations (25, 50,75, 100) mg/ml. The concentration of 100 mg/ml showed a high action against the two species. The combination effect of the purified catechin was estimated with both Amikacin and Gentamycin on the resistant isolates to these antibiotics (E.coli and K.pneumoniae) and has been compared to the minimum inhibitor concentration of the isolates before and after the confusion of antioxidants, as the effect of mixing Amikacin and Gentamycin with Catechin, it gave a synergic effect with Amikacin on two isolates, either Gentamycin gave synergic effect on K.pneumoniae.

جمعت 200 عينة إدرار من مرضى مصابين بالتهاب المجاري البولية الحاد والمزمن, عُزلت 85 عزلة بكتيرية منها, وشخصت بأستخدام الفحوصات الكيموحيوية واختبار العدة API, ,وكان معظمها يعود الى E.coli بنسبة(23.5%) وK.pneumoniae بنسبة(17.6%). أختيرت العزلات البكتيرية E.coli وK.pneumoniae على أساس ترددها الأكثر في إصابات المجاري البولية ومقاومتها لتأثير المضادات الحيوية. درس التأثير المثبط للكاتشين Catechin المنقى من الشاي الأخضر في نمو العزلات عند التراكيز (25, 50100 ,75 ,) ملغم/مليلتر, وقد أظهر التركيز(100) ملغم/ مليلتر فعالية ضد كلا الجنسين من البكتريا. قدر التأثيرالخلطي المركب الكاتشين المنقى Catechin)) مع المضادينAmikacin وGentamycin على العزلات البكتيرية المقاومة للمضادين وهما E.coliوK.pneumoniae وقد تمت مقارنة التركيز المثبط الادنى للعزلات قبل وبعد الخلط للمضادات, اذ كان تأثير خلط Amikacin مع الكاتشين (Catechin) تآزرياً مع مضاد Amikacin على العزلتين , اما مضاد Gentamycin فقد اعطى تأثيراَُ تآزرياعلى بكتيريا K.pneumoniae.

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