research centers


Search results: Found 9

Listing 1 - 9 of 9
Sort by

Article
ROLE OF ALCOHOLIC TURMERIC (CURCUMA LONGA) EXTRACT IN OUTCOME OF IN VITRO SPERM ACTIVATION FOR INFERTILE PATIENTS

Author: Muhammad-Baqir MR Fakhrildin محمد باقر محمد رشاد فخر الدين
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 4 Pages: 301-307
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

BackgroundSemen samples are prepared for assisted reproduction by selecting a population of highly motile, morphologically normal sperm and removing the seminal plasma, leukocytes and bacteria. Culture media provide the spermatozoa with needs that maintain optimal function of spermatozoa to give rise excellent results during semen preparation.ObjectiveThe aim of this study was to investigate the effect of alcoholic extraction of turmeric (AET) on sperm parameters during in vitro sperm activation (ISA) for asthenozoospermic (AZ) and oligoasthenozoospermic (OAZ) patients.MethodsSeventy four infertile patients were included, and classified into two groups according to their sperm parameters. Semen sample was divided into 3 aliquots. One mL of Earl's medium either alone (control group) or supplied with one concentration of AET (5 µg/mL or 10 µg/mL) was over layered the pellet, and the three tubes were incubated at 37 oC for 30 min in air incubator. Sperm concentration, motility, grades activity, progressive motility, normal morphology and agglutination were assessed pre- and post-activation in vitro.ResultsResults revealed an enhancement of most sperm parameters for control and both treated groups post-activation as compared to pre-activation. Post-ISA, sperm parameters for both treated groups were better than the control group. However, best results for improvement of sperm parameters were assessed within treated group (5 µg/mL of AET).ConclusionsThe lower concentration of alcoholic turmeric extraction enhanced human sperm parameters during ISA without any harmful effects on sperm physiology. The results are also useful as a guide for further standardization of turmeric extracts used for pharmaceutical purposes in the techniques of assisted reproduction.Key words Male infertility, Sperm activation, Turmeric.


Article
A new simple medium for in vitro sperm activation of asthenozoospermic patients using direct swim-up technique
وسط زرعي جديد لتنشيط النطف خارج الجسم للمرضى المصابين بوهن النطف باستخدام تقنية سباحة النطف للأعلى مباشرة

Loading...
Loading...
Abstract

This study was designed to compare the results of sperm parameters for asthenozoospermic patients pre- and post-activation in vitro using a new simple medium and direct swim-up technique.Fifty asthenozoospermic patients were involved in the present study with age within range 22-41 years and duration of infertility 2-10 years. From each patient, semen sample was taken and seminal fluid analysis (SFA) was done. A new simple medium was prepared based on Ringer solution supplemented with additives sodium pyruvate and human serum albumin (5% or 10%) which referred as SMART medium (Simple Medium for Assisted Reproductive Techniques; SMART). SMART and Earl's media were used for in vitro sperm activation by direct swim-up technique. Sperm concentration, sperm motility (%), sperm grade activity (%), normal sperm morphology (%) and sperm agglutination (%) were assessed. Crude data were statistically analyzed.From the results of present study, it was appeared that most sperm parameters were significantly (P<0.05) enhanced post-activation in vitro as compared to pre-activation using SMART medium by direct swim-up technique. After in vitro sperm activation, the percentages of progressive sperm activity with grade A, total progressive sperm motility (grades A and B) and normal sperm morphology were significantly increased (P<0.05) in both treated groups as compared to control groups. However, highly significant (P<0.01) and positive correlations for sperm concentration and normal sperm morphology (%) were observed among groups of pre- and post-activation.It was concluded that the SMART medium was suitable for enhancement of sperm parameters of asthenozoospermic patients using direct swim-up technique. Further studies are needed to evaluate effects of SMART medium on successful rate of intrauterine insemination.

صممت هذه الدراسة للمقارنه بين نتائج متغيرات النطف للمرضى اللذين يعانون من وهن النطف قبل وبعد تنشيط النطف خارج الجسم باستخدام وسط زرعي جديد وبسيط وتقنية سباحة النطف للأعلى مباشرة.اشترك خمسين مريضا مصابا بوهن النطف في هذه الدراسة الحاليه وبعمر يتراوح من 22ـ 41 سنه وفترة عقم 2ـ 10 سنه . اخذت عينة السائل المنوي من كل مريض واجري لها فحص السائل المنوي. حضر وسط زرعي بسيط وجديد بالاعتماد على محلول رنكر (Ringer solution) واضافة بيروفت الصوديوم (Sodium pyruvate) و زلال المصل البشري ( Human serum albumin ؛تركيز %5 او %10) وقد اصطلح له مختصر وسط سمارت (SMART medium ؛مختصر وسط زرعي بسيط للتقنيات المساعده على الأنجاب). استخدم و سطي سمارت (SMART) و ايرلز (Earl's) لتنشيط النطف خارج الجسم باستخدام تقنية سباحة النطف للأعلى مباشرة. تم تقييم تركيز النطف والنسبة المئويه للنطف المتحركه والنسبة المئويه لنشاط النطف والنسبة المئويه لشكلياء النطف الطبيعي والنسبة المئويه لتكتل النطف. كما تم تحليل النتائج الأوليه احصائياً. أظهرت نتائج الدراسة الحاليه تحسن معنوي (P<0.05) في أغلب متغيرات النطف بعد التنشيط خارج الجسم عند المقارنه مع قبل التنشيط باستخدام وسط سمارت وتقنيه سباحة النطف للأعلى مباشرة. حصلت زياده معنويه (P<0.05) بعد تنشيط النطف في النسب المئويه لكل من الحركة التقدميه (A) للنطف والحركة التقدميه الكلية (A + B) للنطف وشكلياء النطف الطبيعي لكلا مجموعتي المعامله (وسط سمارت) عند المقارنه مع مجموعتي السيطرة (وسط ايرلز). وعلى اي حال ظهرت علاقات عالية المعنويه (P<0.01) وايجابيه لكل من تركيز النطف وشكلياء النطف الطبيعي لكل مجاميع السيطرة والمعاملة بين قبل وبعد تنشيط النطف خارج الجسم .يمكن الاستنتاج ان وسط سمارت مناسباً لتحسين متغيرات النطف للمرضى المصابين بوهن النطف باستخدام تقنية سباحة النطف للأعلى مباشرة. هنالك حاجه لأجراء دراسات اضافية لتقييم تاثيرات وسط سمارت على معدل نجاح التنميه داخل الرحم.


Article
Anti sperm Antibody in a Sample of 50 Infertile couples
اضداد الحيامن في عينة من 50 زوج وزوجة يعانون من العقم

Author: Areej-A-Jabbar د.اريج جبار
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2011 Volume: 24 Issue: 2 Pages: 132-137
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: Human immunological infertility may be caused by different factors but the major factor is the presence of antis perm antibodies, this might be then a cause of unexplained infertility; alternatively it might be an additional factor in cases of explained infertilityPatients &Methods: A cross sectional study was carried out during the last six months of 2009 for 50 infertile couples they were examined clinically and investigated for assessment of their infertility state, Their sera were tested for positive anti sperm anti body titer by ELISA test, the results were statistically analyzed to evaluate the association of the positive results with the age in years ,duration and cause of infertility ,previous exposure to surgery and poly cystic ovarian syndrome in wives. Results: The percentage of positive serial anti sperm anti body wives in the sample was equal to what was found in husbands (22%). An obvious association was found with the secondary infertility in both genders, in previous exposure to surgery and with long duration of infertility although statistically not significant. The husbands with unexplained infertility had a higher significant association (32.1%) than other infertile husbands. The wives with poly cystic ovarian syndrome showed for the first time (up to our knowledge) a strong association with positive anti sperm anti body titer although statistically not significant. The immunological infertility had no significant association with age although it was higher with old age wives 40 years and more (33.8%) and between 30-39 years husbands (31.8%). Conclusion: The immunological type of infertility is an important finding in unexplained infertility. Infertility nowadays had an obvious correlation with the positive anti sperm anti body titer that can ring a bell to consider the immunological factor an important cause to investigate in both infertile wives & husbands, and to encourage arrangement of further researches with increased sample size and related parameters. Key words:-Immunological infertility. Anti Sperm Anti Body

المقدمة:العقم المتسبب عن المناعة في الانسان قد يتسبب من عوامل مختلفة ولكن العامل الاكبر هو وجود اجسام مضادة للحيامن والتي قد تكون هي المسببة للعقم المجهول السبب او قد تكون سببا اضافيا للعوامل الاخرى المسببة للعقم.طريقة البحث:اجريت هذه الدراسة الجماعية خلال الاشهر الستة الاخيرة لسنة 2009 لخمسين من الازواج الذين يعانون من العقم .حيث تم فحصهم وتقييم اسباب العقم لديهم سريريا ومختبريا ثم سحبت كمية كافية من الدم لكل مشارك وفحصت العينات بطرقة (ELIZA) للتحري عن وجود الاجسام المناعية المضادة للحيامن ,سواءا عند الازواج او الزوجات .وبعد اجراء التحليل الاحصائي حددت العلاقة بين وجود النسب الموجبة في مصول الزوجين وعلاقتها بعمر كل منهما بالسنين مع علاقتها بمدة المعاناة من العقم وسبب العقم ونوعه ومع خلفية تعرض المريض لاجراء عملية جراحية سابقة ام لا ومع وجود التكيس المبيضي لدى الزوجات.النتائج:اظهرت النتائج المستخلصة من هذه الدراسة ان نسبة الزوجات اللواتي يعانين من وجود اجسام مضادة للحيامن 22% وهي مساوية لنسبتها لدى الازواج22% .وجدت في هذه الدراسة علاقة واضحة بين النتائج الموجبة لوجود اجسام مضادة للحيامن في دم الزوجات والازواج وبين تعرضهم لتداخل جراحي سابق وكذلك مع العقم الثانوي ومع طول فترة المعاناة من العقم . على الرعم من انها غير مؤكدة احصائيا.اما الازواج الذين لم تظهر لديهم أسباب واضحة للعقم فقد وجدت لديهم اعلى نسبة مؤكدة احصائيا من الاجسام المضادة للحيامن (32.1%) ولكن الزوجات اللواتي يعانين من التكيس المبيضي اظهرن علاقة واضحة ولأول مرة حسب معلوماتي مع وجود الأجسام المضادة للحيامن لديهن على الرغم من ان ذلك لم يثبت إحصائيا.كما ان النسب الموجبة لتواجد الأجسام المضادة للحيامن لم تظهر علاقة وثيقة مع عمر كلا الجنسين على الرغم من انها كانت اكبر لدى الزوجات في سن الأربعين سنة والأزواج في سن 30-39سنة (33.8%)و (31.8%) على التواليالاستنتاج:ان المناعة المسببة للعقم تعد من الأسباب المهمة للعقم المجهول السبب التكيس المبيضي لدى الزوجات كسبب شائع للعقم في الوقت الحاضر اظهر علاقة واضحة مع تواجد الأجسام المضادة للحيامن لديهن والذي يمكن أن يؤكد إحصائيا بزيادة حجم العينة في دراسة أخرى مستقبلا وذلك مما يقرع جرسا منبها لاعتماد السبب المناعي كعامل مهم يدعو لإجراء الفحوص المناعية لكلا الجنسين من مرضى العقم وإجراء دراسات مستقبلية أوسع وأكثر تطورا في هذا المجال.


Article
Study of the effect of decorticated and defatted Castor Seeds (Ricinus Communis Linn.) on sperm functions and characters of male mice.

Authors: Mustafa A. Jasim --- Farid J. O. Al-Tahan
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2011 Volume: 7 Issue: 3 Pages: 192-204
Publisher: Diyala University جامعة ديالى

Loading...
Loading...
Abstract

This study was designed to investigate the effects of decorticated and defatted castorseeds (Ricinus Communis) on the sperms functions and characters of male mice at twoconsecutive spermatogenic cycles. Eighteen albino Swiss male mice with Ten weeks old wereused as animal model, they divided in to three groups (Six male mice of each), 1st groupserved as control group and received distilled water only, and the 2nd group has been set astreatment group which was treated orally with 1.76 mg/kg. BW from watery suspension ofdecorticated and defatted castor seeds extract for 38 days (single spermatogenic cycle). The3rd group has been treated with the same protocol of the 2nd group but was allowed a recoveryperiod (free from the treatment) of another 38 days (double spermatogenic cycles). Themeasured parameters were: total sperm count, Sperms viability, abnormal sperm percentage,and the turbidimeteric parameters of sperm motility.


Article
Analysis of DNA Damage and Oxidative Stress in Human Spermatozoa and Some Biochemical Changes in Seminal Plasma and their Correlation with Semen Quality of Infertile Men

Authors: Ghassan T. Alani --- Sermed S. Khonda --- **,Hedef D. El Yaseen
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2011 Volume: 10 Issue: 1 Pages: 81-88
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:Numerous studies have shown the presence of DNA strand breaks in human ejaculated spermatozoa. The nature of this nuclear anomaly and its relationship to patient etiology is however poorly understood.OBJECTIVE:This study was done to investigate the relationship between nuclear DNA damage, assessed using the TUNEL assay and a number of biochemical parameters including zinc level, copper level, and the malondialdehyde (MDA) level as an indicator of the lipid peroxidation and also the standard general seminal fluid examination.MATERIAL AND METHODS:In this study 100 seminal samples were tested. Seventy five infertile patients aged (30.33±5.96) and 25 normal people aged (29.84±6.434) were included as a control. All tests were done to all the samples except the DNA fragmentation which was done to 60 samples only (45 patients and 15 normal).RESULTS:A positive relationship between DNA damage level and infertility was found. Also there were higher levels of MDA level and DNA damage in spermatozoa of infertile patients (P=0.001). We found also absence of difference in the copper level between the two groups. Regarding seminal zinc level we found that there was a low zinc level in infertile patients (P=0.001). Non-significant correlations between zinc level and the percentage of DNA fragmentation and the MDA levels were obtained.CONCLUSION:This study suggested that sperm DNA analysis might be better discriminator between infertile and fertile men than standard semen analysis also oxidative stress and sperm DNA damage contributed to sperms dysfunction. The infertile samples associated with high levels of oxidative stress and low seminal zinc levels.


Article
Effects of L-Arginine, Vitamin E and Their Combinations on Sperms Morphology in Albino Male Mice

Authors: Raza H. Husein --- Mahmood O. Ahmed --- Sulaf M. Muhammed
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2011 Volume: 14 Issue: 2 Pages: 137-143
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

AbstractThis study designed to evaluate the effected of L-arginine, vitamin E and their combinations on sperms morphology (sperm quality). Twenty (20) male albino mice was included in this study and dividing into four groups (Control group, Vitamin E treated group, L-arginine treated group, and Vitamin E+ L-arginine treated Group). The animals were kept under suitable conditions of temperature (24±2 ْ C) and light program (12-14 hours/ day). Results revealed that mice treated with vitamin E + L-arginine (0.80 ± 0.141) showed a significant (p≤0.05) decrease in numbers of abnormal sperm without head, treatment mice with vitamin E + L-arginine (1.68 ± 0.10) gave a significant decreased in numbers of abnormal sperms without tail, mice treated with vitamin E (1.00 ± 0.38) and (L-arginine + vitamin E) (0.52 ± 0.14) significantly decreased sperms without hook, furthermore mice treated with vitamin E (11.04+1.63) significantly decreased sperms defective head when compared with control groups. the period of experiments were 30 days. The percentages of abnormal sperm morphology were calculated by counting 200 spermatozoa.

الخلاصةصممت الدراسة لتقييم تأثير L-Arginine، فيتامين E وتداخلاتهما فى مورفولوجي الحيامن (نوعية الحيامن). تضمنت الدراسة عشرون ذكرا من الفئران البيضاء قسمت الى أربعة مجاميع (مجموعة السيطرة، مجموعة عوملت بفيتامينE ، مجموعة عوملت بـ L-Argininاما المجموعة الأخيرة فقد عوملت بفيتامين E مع L-Arginine). وضعت حيوانات التجربة تحت ظروف مناسبة من حرارة (24± 2 درجة مئوية) وفترة ضوئية (12-14) ساعة/يوم. اظهرت النتائج بان فئة الفئران المعاملة بفيتامينE مع L-Arginine (0.8 ± 0.141) كان عدد الحيامن المشوهة فيها (بلا رأس وبلا ذيل) معنويا (0.05> P) اقل عددا من الحيامن المشوهة في فئة السيطرة.وصلت اعداد الحيامن المشوهة بدون ذيل (1.68±0.1) و الفئران المعاملة بفيتامين E (1± 0.38) بالاضافة الى فيتامينE مع L-Arginine (0.52 ± 14) وأدت الى انخفاض معنوي في الحيامن بدون كلاب. بينما الفئران المعاملة بفيتامين Eفقط سببت انخفاضا معنويا فى أعداد الحيامن المشوهة بدون رأس مقارنة مع مجموعة فئران السيطرة. عوملت جميع الفئات لمدة ثلاثون يوم. تم حساب النسبة المئوية لكل فئة تمت دراستها باستخدام مائتي حيوان منوي.


Article
Correlation between intra uterine insemination outcome and Kruger strict criteria using two staining methods to detect the sperm morphology of infertile men
العلاقة بين نتائج التلقيح الاصطناعي والمعايير المحددة لطريقة كروكر باستخدام نوعين من الصبغات لتحديد شكلياء النطف في الرجال العقيمين

Author: saad s. aldujaily سعد صالح الدجيلي
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2011 Volume: 1 Issue: 1 Pages: 12-20
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

BackgroundSince 1999 , the World Health Organization (WHO) recommended the assessment of sperm morphology by Kruger strict criteria(KSC), The manual of WHO contains the cut off values of sperm morphology measured by KSC for normal subject and patients treated by in vitro fertilization (IVF) and intra- I cytoplasmic sperm injection(ICSI) without any information regarding intrauterine insemination( IUI). ObjectivesThe objective of this study is to achieve two major goals that are :1-Assessment of sperm morphology by applying KSC , using two different biological stains, named commercially Testsimplets {TS)pre-stained slides and Sperm-O-Scan (SOS)stain 2-To have a cut off value for morphologically normal sperm that resulting into a successful IUI outcome.Materials and Methods;Fifty five unselected couples attending the Institute of Embryo Researches and Infertility Treatment were included in this prospective study from December 2007 til) August 2008 ,The mean age of male partner was 31,7±1-87years with an average duration of 6<25±1.65years of infertility. The women were prepared for IUI. All the males were subjected to semen analysis with in vitro sperm I activation using layering swim-up technique. Assessments of the morphologically normal sperm(MNS) of the samples were performed by using both, High power field and KSC by using TS and SOS stains before and after the activation. Correlation of the positive IUI results and MNS by KSC waw recorded denoting the cut off value for successful results.ResultsThe mean of morphologically normal sperm applying KSC with the use of SOS Stain before in vitro sperm activation was 16.10% and after activation was 37.85% and by using TS stain before in vitro sperm activation was 15.14% and after activation was 37.09% No significant (P>0.05) differences was detected between the results of MNS of the two stains. The result of IUI was 11 positive pregnancies out of the 55 cases. The mean of MNS using KSC for these positive cases was 11.58% and 12.84% for SOS and TS stains, respectively.ConclusionsThe cut off value of MNS by applying KSC and using two different I biological stains for a successful IUI program was 11.58% by SOS stain and 12.84% by TS pre-stained slides. Although the study found no statistical differences between the two stains, TS found to be easier, I simpler and less time consuming technically than SOS stain.


Article
Effect of Glycyrrhiza glabra extract on in vitro sperm activation and embiyonic development following intra-peritoneal insemination in mice: experimental model for mammals
تاثير مستخلص عرق السوس على تنشيط النطف والتطور الجنيني بعد التلقيح عبر الغشاء البريتوني في الفئران:موديل تجريبي للبائن

Authors: saad s.aldujaily سعد صالح الدجيلي --- zena muzher زينة مزهر
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2011 Volume: 1 Issue: 2 Pages: 31-38
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Glycyrrhizaglabm(Gg) is a herbal medicine used in the treatment of different syndromes. Recently, the effect of this plant on the reproductive system has been studied. However, its role in in vitro still not dear.objectiveThe present study aims to investigate the possibility of using Glycyrrhiza glabra extract for in vitro sperm direct activation, and its effect on in vivo fertilization rate (FR) and early embryonic development (ED) following intra-peritoneal insemination (IPI ).The mice were used as an experimental model for mammals.Materials and methods:Glycyrrhiza glabra extract (2 mg/rnl culture medium) was used fix in vitro direct sperm activation technique. The female mice were divided into two groups: spontaneous^ ovulated (SQM) and superovulated groups (SuOM).Each group was divided into two subgroups, the first: the IPI was accomplished by epididymal sperm activated in vitro by adding 20%Gg to ihe culture medium .The second subgroup: the fP! is accomplished without adding Gg to Ihe culture medium.Revuein vitro activation of epididymal sperms with 20% Gg has shown positive effects on sperm concentration, sperm motility, and grade activity of progressive forward movement. There was a significant (P


Article
GENETIC AND PHENOTYPIC PARAMETERS FOR SPERM – EGG PENETRATION AND SOME SEMEN TRAITS FOR WHITE LEGHORN COCKS .
المعالم الوراثية والمظهرية لقابلية اختراق النطفة للبويضة وبعض صفات المني في ديكةاللكهورن الأبيض.

Loading...
Loading...
Abstract

This study was conducted at the Poultry Farm of the Animal Resource Department , College of Agriculture , Baghdad University , during the period from 8/10/2007 to 28/3/2009 , to study the genetic evaluation of White Leghorn flock for selection according to sperm – egg penetration , semen characteristics , fertility and hatchability traits , egg production traits ( weight of first egg , egg weight , egg production at the first 100 days of production , egg mass and the body weight at sexual maturity) and histological testis traits , after the adjustment of fixed effects , and estimate of its genetic parameters.The General Linear Model –GLM procedure ,within the SAS program was used to study the effect of fixed factors , and to determined the random effect by using Restricted Maximum Likelihood – REML, for sire (24) , dams (114) . These values were ranked in descending order for selection purpose. Results obtained can summarized as follows :1-Means of sperm – egg penetration was 74.25 hole / 1.5 mm2 and for other semen traits were 3.98 x 109 ml for semen concentration 20.13% for spermatocrit , 0.44 ml for semen volume , 82.19% for mass motility , 84.99% for individual motility , 11.09% for percentage dead spermatozoa , and 10.41% for the percentage of abnormal spermatozoa, Whereas the heritabilities and repeatabilities for these traits were (0.79 , 0.86) , (0.34 , 0.42) , (0.25 , 0.29) , (0.19 , 0.34) , (0.26 , 0.48) , (0.18 , 0.44) , (0.14 , 0.37) and (0.36 , 0.43) , respectively .2-The effect of season on traits include in this study was highly significant (P < 0.01) in winter than in summer .3-There was high positive genetic and phenotypic correlations between SP with semen concentration ,(0.42 , 0.45) spermatocrit (0.89 , 0.91 ), semen volume (0.34 , 0.37 ), mass motility ( 0.92 , 0.95 ), and individual motility (0.91 , 0.92 )

أجريت هذه الدراسة في حقل الطيور الداجنة التابع لقسم الثروة الحيوانية – كلية الزراعة – جامعة بغداد للمدة من 8/10/2007 ولغاية 28/3/2009 ، لعينة من قطيع اللكهورن الابيض اعتماداً على صفة قابلية اختراق النطفة للبيضة وصفات المني الاخرى ، لأغراض الانتخاب بعد التعديل لتأثير العوامل الثابتة (الموسم والمعاملة) وتقدير المعالم الوراثية للصفات المدروسة.استعملت طريقة الانموذج الخطي العام (General Linear Model-GLM) ضمن البرنامج الاحصائي SAS لتحليل تأثير العوامل الثابتة ( (Fixed effect ، ولتقدير مكونات التباين للتأثيرات العشوائية (Random effect) نفذت طريقة تعظيم الاحتمالات المقيدة (Restricted Maximum Likelihood – REML) .استخدم في هذه الدراسة 38 ذكرا و 114 انثى.توصلت الدراسة الى جملة من النتائج يمكن اجمالها بما يلي :1-بلغ المعدل العام لقابلية اختراق النطفة للبيضة 74.25 حفرة / 1.5 ملم2 و لصفات المني 3.98 ×109 / مل لتركيز النطف و20.13% لحجم النطف المضغوطة و 0.44 مل لحجم القذفة و 82.19% للحركة الجماعية للنطف و 84.99% للحركة الفردية للنطف و 11.09% لنسبة النطف الميتة و 10.41% لنسبة النطف المشوهة. بلغ تقدير المكافئ الوراثي للصفات اعلاه 0.79 و 0.34 و 0.25 و 0.19 و 0.26 و 0.18 و 0.14 و 0.36 وبالتتابع.2-كان لموسم السنة تأثير عالي المعنوية (أ > 0.01) في جميع الصفات التي شملتها الدراسة ، اذ تفوق موسم الشتاء على الصيف الذي كان تأثيره سلبياً في مجمل الصفات.3-لوحظ ارتباط وراثي و مظهري موجب و عالي المعنوية ( أ > 0.01 ) بين كل قابلية اختراق النطفة للبيضة وتركيز النطف ( 0.42 و 0.45 )و حجم النطف المضغوطة ( 0.89 و 0.91 ) وحجم القذفة ( 0.34 و 0.37 ) و الحركة الجماعية للنطف ( 0.92 و 0.95 ) و الحركة الفردية للنطف ( 0.91 و 0.92 ) .

Listing 1 - 9 of 9
Sort by
Narrow your search

Resource type

article (9)


Language

English (8)

Arabic (1)


Year
From To Submit

2011 (9)