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Article
Effect of Stress on the Composition and Flow Rate of Saliva

Authors: Nahla OM Tawfik --- Isra'a H Al-Hamdani --- Karama M T Al-Nuaimy
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2012 Volume: 12 Issue: 1 Pages: 66-70
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To assess the effect of acute psychological stress on some salivary glands functions. Materials and Methods: Twenty-three undergraduate dental students participated in this study. They were asked to provide two samples of unstimulated whole saliva for 5 minutes, the first sample before amid- year oral academic examination and subsequently the second, one month later in a non stressful situation (after holiday break) as control group. Salivary flow in one minute was determined, in addition, total protein, albumin, uric acid and calcium levels in saliva were assessed using determination kits method from Biolabo (France). Results: The results showed a significant stress mediated decrease in the sali-vary calcium concentration (0.230.21 mmol/L) by -78.50% in comparison with non stressful condi-tion. Salivary flow level (0.62  0.28 ml /min) and albumin concentration (16.3913.69 mg/100ml) were decreased by (- 4.62% and -37.30% respectively), while uric acid (1.671.13 mmol/L) and total protein concentrations (1.030.69 g/100ml) were elevated by 9.56% and 10.60% respectively. Not all these changes were statically significant. Conclusions: These results suggest that the acute psychologi-cal stress exerts its influence on salivary composition and this will increase the value of saliva as dy-namic biological fluid in controlling the oral health.

Keywords

Saliva --- stress --- total protein --- albumin --- uric acid --- calcium


Article
Salivary vitamins and total proteins, in relation to cariesexperience and gingival health, according to nutritional status of a group of five-year old children

Author: Nada J. Radhi ندى راضي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 3 Pages: 129-136
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Malnutrition influences the development of the teeth and the formation, function and secretion of thesalivary glands, which in turn influence susceptibility to dental caries and gingival disease. The aims of this study wereto assess the salivary antioxidants (vitamin A, C and E) levels as well as total protein and their relation to cariesseverity and gingival health status among mal- and well-nourished children.Materials and Methods: The sample consisted of 60 children and they divided according to nutritional status (30malnourished and 30 well nourished). The 2000 Centers for Disease Control and Prevention (CDC) growth charts wasused for assessment of nutritional status (height for age). The age was five years old. Caries severity (d1-4s) wasassessed according to Muhlemann (1976). Dental plaque recorded following the criteria described by Sillness andLöe (1964). The gingival index (GI) was used according to Löe and Sillness criteria (1963). Stimulated whole salivasamples were collected and chemically analyzed by using colorimetric method to determine the salivaryantioxidants (vitamin A, C and E) and total protein. All data were analyzed using SPSS version 18.Results: Results recorded a higher mean value of dmfs among malnourished in comparison to well nourished withstatistically highly significant difference (P< 0.001). According to grades of lesion severity, d4 was significantly thehigher among malnourished children (P< 0.001). Strong highly significant correlations were noticed between ds, dmfsand PI among malnourished and well nourished children. Significantly lower values of vitamins and total protein werenoticed among malnourished children compared to well nourished (P< 0.001). Negative highly significantcorrelations were found with all vitamins among malnourished children regarding caries-experience and GI.Conclusion: Childhood chronic malnutrition (stunting) is associated with salivary hypofunction. This may act as a riskfactor for dental caries and gingival disease in the target group.

Keywords

vitamin A --- C --- E --- total protein --- dental caries --- gingival disease


Article
A cross-sectional tri-level study of the obesity effects on the salivary uric acid and total protein of gingivitis Iraqi subjects

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Abstract

Background: Obesity is the fastest growing health-related problem in the world. It plays an affecting role in thebiochemistry of many serious systemic diseases like diabetes and CVD. Periodontitis appeared to have a reciprocalrelationship with both, obesity on one hand side and the serious systemic diseases on other hand. The aim of study: isto investigate the inter linking between obesity and gingivitis by screening the salivary uric acid and total protein.Material and method: Control healthy periodontal and systemically-normal weight,27 male, ( CG), gingivitis–normalweight, systemically healthy(GN) 16 male, Gingivitis-overweight-systemically healthy(GOV) 14 male).and Gingivitisobese-systemically healthy(GO)12 male, aged 30-40 y, no smokers, Their weight measured according to BMI.Clinical data were recorded according to PL.I, GI, BOP, at the same hour of unstimulated saliva collection.Results: Obese subjects showed increased PL.I, G.I, BOP scores and high significant increase of salivary total protein.BOP; showed significant increase in bleeding sites as the Body Mass Index goes up. Uric acid showed, always,negative correlation with the totality of gingival inflammation parameters, they were significant only with normalweight subjects.Conclusion: Total protein. Appeared significantly positive correlated to gingivitis more than to the obesity. While Uricacid correlated insignificantly negative with the gingivitis but not to the increased body weight.The increased bodyweight also positively correlated to the gingival inflammation, these results could say that gingivitis modifies the salivarychemicals while the obesity enhances gingivitis


Article
Effects of diabetes mellitus types II on salivary flow rate and some salivary parameters (total protein, glucose, and amylase) in Erbil city

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Abstract

Background: The concentration of some components of saliva may be associated with certain systemic illnesses,reflecting the hormonal, immunological, neurological, emotional, nutritional and metabolic states of the patient. Theaim of this work was to assess salivary flow rate, and to evaluate saliva samples for levels of salivary total protein,glucose, and alpha amylase, in diabetics type II and healthy subject in both genders.Subjects and methods: Unstimulated salivary flow rate, salivary total protein, glucose, and amylase were measuredin 90 subjects, 60 with diabetes mellitus type II (30 controlled and 30 uncontrolled diabetic patients) and in 30 healthysubjects.Results: Significant difference in salivary flow rate in diabetic patient when compared with healthy subject. Thefinding showed no significant differences between salivary total proteins in all groups. Significant difference in salivaryglucose and amylase concentration was found between the healthy subjects and type 2 diabetic patients.According to the gender, there were only significant differences between male and female in salivary flow rate forhealthy subjectsConclusions: Patients with type 2 diabetes mellitus have higher concentration of salivary glucose and lower value ofsalivary flow rate and amylase. No significant difference was seen in protein value in all groups


Article
The effect of magnetically treated water on the total protein and some enzymes in the muscle of Cyprinus carpio L.
تأثير الماء المعالج مغناطيسيا على البروتينات الكلية وبعض الانزيمات في عضلات سمكة الكارب Cyprinus carpio

Authors: A. A. Thaker عبد علي ذاكر --- A. S.Faris علي شنيار فارس --- F. jassim فهام جاسم محمد
Journal: ANBAR JOURNAL OF AGRICULTURAL SCIENCES مجلة الأنبار للعلوم الزراعية ISSN: PISSN: 19927479 / EISSN: 26176211 Year: 2012 Volume: 10 Issue: 1 Pages: 213-222
Publisher: University of Anbar جامعة الانبار

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Abstract

AbstractThe fish Cyprinus carpio L.(24-25gm) were exposed to the water magnetically treated with different magnetic intensities (750, 1500, 3000) Gauss, for a period of 8 days. The muscle was used to follow the changes in the total proteins and electrophoresis proteins, as well as the activity of some enzymes (Alkaline phosphates, Aspartate transaminase, Alanin transaminase and Acid phosphates) using spectrophotometric methods. The electrophoresis method was also used to follow up the effects on the Esterase bands which stained by α-naphthyl acetate .The results showed an increase in the activities of the enzymes in the muscle when exposing the fish to the magnetized water. The number and intensity of electrophoresis esterase bands didn’t change.

تم تعريض اسماك الكارب الشائع ) Cyprinus carpio 24-25 غم ) إلى ماء معالج مغناطيسيا" بشدد مغناطيسية مختلفة 750 و1500 و3000 كاوس لفترة 8 أيام. استعملت العضلات لمتابعة التغيرات التي تطرأ على كمية البروتينات الكلية والبروتينات المرحلة كهربائيا وكذلك فعالية كل من اﻹنزيماتAlkaline phosphates وAspartate transaminase و Alanin Transaminase وAcid phosphates بأستخدام الطريقة الطيفية. كما استخدمت طريقة الترحيل الكهربائي لمتابعة التأثيرات على طرز انزيم الأستريز Esterase المرحلة على الهلام المتعدد الاكريلامايد والمصبوغة بواسطةα-naphthyl acetate . وقد أظهرت النتائج أرتفاع فعالية الانزيمات في العضلات عند تعريض الاسماك الى الماء الممغنط ولجميع الشدد ، ولم تظهر تغيرات في عدد او كثافة حزم إنزيم الاستريز .

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