research centers


Search results: Found 2

Listing 1 - 2 of 2
Sort by

Article
Preparation of Vaccine against Diabetic Foot Pathogenic Bacteria Using Low Level Diode Laser

Author: Zainab Awad Radi (M.Sc) a Ihsan F. Rostum (M.Sc, PhD) b *Sadiq Abbas Al-Mukhtar (FICMS) c
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2013 Volume: 9 Issue: 2 Pages: 29-31
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Since the invention of laser in 1960, lasers have been developed and approved in many fields. Lasers can now be regarded as practical tools with unique properties that have been utilized effectively in several applications in fields of medical and biological sciences.Objectives: The aim of the current study was to preparation of vaccines (live attenuated and killed) by irradiation of the bacteria by the low level diode laser.Methods: six bacterial isolates were isolated from human samples of diabetic foot infections, which used for preparation of vaccines. The experiment was conducted on fifteen adult male rabbits; they were divided into three groups with 5 rabbits each. Blood samples were collected from the marginal ear vein of the rabbits after one month of the vaccination, for the purpose of measuring the concentration of the immunoglobulins which present in their serum, using Radial Immunodiffusion (RID) method by specialized kits (LTA-Italy).Results: After irradiation of the bacterial suspensions with the diode laser for different exposure times and different frequencies, and the wavelengths used were (660, 820, 915) nm, the growth of bacterial isolates decreased until killed of bacteria at 40 min. The results of IgA concentrations for the three groups were highly significant (P < 0.01) when comparing the attenuated with control group, while were significant (P < 0.05) between the killed vaccine and control groups, and not significant variations between the attenuated and killed groups,Conclusions: Wavelength (660) nm was more effective in killing the bacteria, and the variations were not significant between the live attenuated and the killed vaccine.


Article
Use Box –PCR to Study Genetic Relatedness of Some Pseudomonaus aeruginosa Bacterial Isolates from Different Clinic and Environmental Sources In Baghdad

Author: Ebtesam H. Nassir University of al Mustansiriyah
Journal: journal of the college of basic education مجلة كلية التربية الاساسية ISSN: 18157467(print) 27068536(online) Year: 2013 Volume: 19 Issue: 77 Pages: 1-8
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Totally twenty six different isolates of Pseudomonas aeuruginosa ( P. aeruginosa ) were collected ,sixteen clinical isolates ( 62% of total group ) were isolated from different samples ( Urine , blood , sputum , wound , ear , eye and throat swabs ) from Medical city hospital in Baghdad, and ten environmental isolates ( 38%of total group ) were isolates from different places of soil and water in the same city . All isolates were diagnosed by bacteriological and biochemical tests . The crud DNA was extracted from all clinical and environmental isolates . BOX-PCR was achieved using BOX1AR primer . The BOX-PCR products were analyzed by horizontal agarose gel electrophoresis . The dendrogram for all isolates was draw by using band-based Jaccard and Dice coefficients where the fingerprints were generated by banding patterns using un weighted pair-group method with arithmetic average ( UPGMA ) , in order to study the relatedness among the clinical and environmental isolates . The results were showed that the crude DNA was suitable to BOX-PCR , and presence of the high similarity in banding patterns of clinical and environmental isolates itselves , which was about 45%-99% and 61%-100% in Jaccard and Dice coefficients respectively . The similarity between these two groups was about 40% and 55% in Jaccard and Dice coefficients respectively . Although , the marked relatedness among all clinical and environmental isolates , this study was concluded presence of genetic variation between the clinical and environmental isolates of this opportunistic bacterium , by using BOX-PCR technique and BOX1AR primer .

Listing 1 - 2 of 2
Sort by
Narrow your search

Resource type

article (2)


Language

English (2)


Year
From To Submit

2013 (2)