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Article
Isolation of Some Bacteria from Chronic Otitis Media

Author: Samira Kgeer Jreemich سميرة غجير جريمخ
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2014 Volume: 10 Issue: 18 Pages: 159-163
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Chronic Otitis media is a very common disease in people of Diwania due to several factors , microbiological , envirnmental and social . Swab samples and data were collected from private surgeries of specialized doctors ( ENT ) . 75 samples were collected , 51 cases were positive ( 68%) and 24 cases were negative ( 32%). Number and percent of infected males 28 ( 54.9%) , while number and percent of infected females 23 ( 45.1%) .The main results showed high percents of infection were in ages between 20-29 years old and 30-39 years old( 31.4 % and 25.5% ) respectively . Six types of bacteria were isolated from the patients , two of them were gram positive ( Staphylococcus aureus and Streptococcus pyogens ) while gram negative were four ( Pseudomonas aeruginosa , Proteus vulgaris , E. coli and Enterobacter cloacae ) . The highest percent of gram positive was S. aureus 90.5% , and the highest percent of gram negative was P.aeruginosa 56.6% ) .Sensitivity test was done to know the best antibiotic can be used as treatment for otitis media . The most effective antibiotics for most causative agents were cefotaxin , co-trimaxozole , gentamycin , tetracycline.

أن مرض ألتهاب الأذن الوسطى المزمن من الأمراض الشائعه بين الناس وذلك بسبب عوامل متعدده منها أحيائيه , بيئيه وأجتماعيه . جمعت نماذج من الأذن المريضه للمراجعين للعيادات المتخصصه بالأذن والأنف والحنجره عدد الحالات 75 حاله منها 51 حاله موجبة لألتهاب الأذن الوسطى (68%) و 24 حاله سالبه ( 32%) . عدد الحالات الموجبة من الذكور 28 ( 54.9%) والحالات الموجبة من الأناث 23 (45.1%) . بينت النتائج الرئيسيه نسب عالية من الأصابه في الأعمار بين 20 – 29 سنه و 30- 39 سنه ( 31.4 % و 25.5 % ) على التوالي , فضلا ً عن نسب عالية سجلت في الذكور ( 54.9 % ) أكثر من النساء . تم عزل ستة أنواع من البكتريا من المرضى , أثنين منها كانت موجبة لصبغة كراموهي ( Staphlococcus aureus , Streptococci pyogens ) بينما البكتريا السالبة لصبغة كرام كان عددها أربعة وهي ( Pseudomonas aeruginosa , Proteus vulgaris , E. coli and Enterobacter cloacae ) . أعلى نسبة بين البكتريا الموجبة لصبغة كرام كانت S. aureus ( 90.5 % ) بينما أعلى نسبة بين البكتريا السالبة لصبغة كرام كانت P. aeruginosa ( 56.6% ) .تم أجراء أختبار الحساسية للمضادات الحيوية وذلك لمعرفة أفضل هذه المضادات لتستعمل كعلاج لألتهاب الأذن الوسطى . أغلب المضادات التي كانت ذو تأثير جيد على البكتريا هي ( Cefotaxin , Co-trimaxozole , Gentamycin, Tetracyclin ).


Article
THE PREVALENCE AND ANTIMICROBIAL RESISTANCE OF PSEUDOMONAS SPECIES IN PATIENTS WITH CHRONIC SUPPURATIVE OTITIS MEDIA

Author: Dr. Zaid Ihsan Al-Attar M.B.Ch.B ,M.Sc. (Pharmacology)
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2014 Volume: 10 Issue: 1 Pages: 114-117
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chronic suppurative otitis media (CSOM) is the result of aninitial episode of acute otitis media and is characterized by a persistent discharge from the middle ear through a tympanic perforation for at least 2 weeks duration. It is an important cause of preventable hearing loss, particularly in the developing world.Methods. 1. To get an overview on the bacterial ear infection profile in general 2. To assess the antibiotic resistance of Pseudomonal infection (PS) particularly since it is usually the commonest infection to cause otitis media and the most difficult to treat due to the problem of multi drug resistance... A cross sectional study was done which included 405 patient of CSOM patients196 (48%) case were males ,209 (52%)case were females. Swabs for aural discharge was taken from those patients. Discharge is cultured by inoculating it into blood , MacConkey agar , chocolate agars and Sabouraud agar (for fungi).If the isolate shows to be Pseudomonas isolate growth then another culture of the isolate is performed on Muller Hinton Agar. Then the antibiotic susceptibility and resistance of Pseudomonas isolate is assessed by (Kirby-Bauer Method)Results:196 (48%) case were males.209 (52%) case were females with a male to female ratio 1:1.1150 (73%) cases were infected with Pseudomonas species (PS) The sensitivity of the Pseudomonas isolates to the following antibiotics is shown below:Amikacin 91.7%Imipenem 89.7%Ceftazidime 81.8%Ciprofloxacin 73.7%Garamycin 72.9%Tobramycin 67.7%Ticarcillin 66.7%Cefoperazone 42.9%Conclusions: Pseudomonas species is the commonest microorganism in cases of CSOM.Microbiological identifications and antibiotic resistance determination of pathogens isolated from the middle ear in patients with CSOM not responding to empirical antibiotic treatment gives possibility of the choice of an effective antibiotic and its proper dosage. Cefoperazone ,a relatively new antibiotic that is used in Iraq to combat pseudomonal infections has proven to be poorly effective compared with other previously used antibiotics.


Article
Microbiological Assessment of Chronic SuppurativeOtitis Media
دراسة الأحياء المجهرية المسببة لالتهابالأذن الوسطى القيحي المزمن

Author: Imadfarhan al-zubaidy
Journal: kufa Journal for Nursing sciences مجلة الكوفة لعلوم التمريض ISSN: 22234055 Year: 2014 Volume: 4 Issue: 3 Pages: 101-105
Publisher: University of Kufa جامعة الكوفة

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Abstract

Background : Chronic SuppurativeOtitis Media is a common infectious disease . the microorganisms commonly associated with and their antibiotic sensitivity patterns is important for its treatment .Objectives : to study the microorganisms associated with chronic suppurativeotits media and their antibiotic sensitivity patterns among our patients . Material and Methods : this descriptive study was carried out from January 2012 to December 2012 at ENT Department and microbiology department . of AL-Hakeem general hospital in Najaf city . A total of 100 patients with unilateral and bilateral active chronic suppurativeotits media attending the out patients clinic were included in the study , pus sample were collected from the discharging ears and sent to microbiological department.Results :- from 100 specimens there were 90 (90%) bacterial isolates and 10 (10%) fungi .Pseudomonas aerugenosa 40 (40%) was the dominant isolate followed by Staphylocooccusaureus 19 (19%) and Proteus spp. 15 (15%) . Antibiotic sensitivity pattern of Pseudomonas aerugenosa showed that imipenem was active in 100% and Vancomycin was active in 100% of Staphylococcus aureus isolate .Conclusion :- Pseudomonas aerugenosa is the most common isolates followed by Staph . aureus from the cutluer specimens of chronicsuppurativeotits media . Pseudomonas aerugenosa is increasingly becoming more resistant to the commonly used antibioltic like cephalosporine Amoxicillin & Ampicillin .Recommendation:We advise to do culture and sensitivity test for every patient with chronic suppurativeotits media (CSOM)and to describe systemic antibiotics according to the result of this test .

خلفية البحث:يعد التهاب الأذنالوسطى القيحي المزمن من الأمراض الخمجية الشائعة كما ان دراسة الأحياء المجهرية المسببة للالتهاب وحساسيتها للمضادات الحيوية لها دور أساسي في علا ج هذا النوع من الأمراض.الهدف: هو دراسة الأحياء المجهرية المسببة لالتهاب الاذن الوسطى المزمن القيحي وحساسيتها للمضادات الحيوية بين المرضى المشمولين بهذه الدراسة . المنهجية: اجريت هذه الدراسة للفترة من كانون الثاني 2012 لغاية كانون الاول 2012 في شعبة الانف والاذن والحنجرة وشعبة الاحياء المحهرية في مستشفى الحكيم العام في النجف . شملت الدراسة مائة مريض مصابين بالتهاب الاذن الوسطى المزمن القيحي تم تشخيصهم سريريا في العيادة الاستشارية , وتم جمع العينات Ear swabs من المصابين ارسلت لدراستها بكتريولوجيا في شعبة الاحياء المجهرية في مختبر المستشفى وتم تحليل النتائج بإعتماد النسب المئوية . النتائج: ظهر من خلال هه الدراسة ان 40% من البكتريا التي تم عزلها كانت من صنف ال Pseudomonas aerugenosa تليها الStaph aureusبنسبة (19%) . اظهر نموج فحص الحساسية للمضادات الحيوية ان الPseudomonas aerugenosa كانت حساسة للمضاد الحيوي ( Imipenem) بنسبة 100% وال Staph aureusكانت حساسة بسنة 100% للمضاد الحيوي (Vancomycin) .الاستنتاجات: تعد الـPseudomonas aerugenosa البكتريا المسببة الأكثرشيوعا في حالات الإصابة بالتهابالأذن الوسطى المزمن القيحي تتبعها الـ Staph aureus . اصبحت البكتريا Pseudomonas aerugenosa مقاومة وبشكل متزايد للمضادات الحيوية شائعة الاستخدام مثل السفالوسبورينات والأوموكسيل والامبسلين . التوصيات: يوصى باجراء فحص الزرع والحساسيه للمضادات الحيويه لكل مريض مصاب بالتهاب الاذن الوسطى المزمن ثم وصف المضاد الحيوي المناسب طبقا لنتائج هذا الفحص .


Article
Effect of Sofrodax, Acetic Acid and Ear Wax on Biofilm Formation on Bacterial Isolates from Otitis Media

Authors: Juman Oday Sabri --- Ilham A. Bnyan --- Safaa H. Al-Terahi
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2014 Volume: 11 Issue: 4 Pages: 961-970
Publisher: Babylon University جامعة بابل

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Abstract

In this study, one hundred otitis media (OM) swabs were collected from (100) patients who were referred to Hilla Teaching Hospital (ENT unit) and privacy clinic during a period (from November 2013 through March 2014) suffering from OM. The collected samples were investigated for bacterial isolation. Bacterial culture was positive in (96%) patient's verses (4%) patients revealed negative bacterial culture. The most common types of bacterial isolated were S. epidermidis (23.6%), followed by S. aureus (17%), Proteus spp. (14.1%), Ps. aeroginosa (9.4%), Streptococcus spp., K. pneumoniae and Enterobacter spp. (7.6%), Acinetobacterbummanii (5.6%), Serratiamarecence (5.6%) and E. coli (0.9%).Biofilm formation was investigated in all bacterial species, and the results showed that the most isolates that form biofilm in gram negative bacteria and gram positive bacteria in high rate (100%).The effect of ear wax, vinegar and acetic acid on biofilm formation were studied the results showed that these materials have some effect on biofilm formation.


Article
Microbiology of active chronic otitis media: in comparison with abroad studies

Author: Dr. Anwar. S. Jaafar FIBMS , MBChB Dr.Raad Aboud AL-Obaidy FICMS, MBChB Dr.Wisam .S.Dawood FICMS, DLO, M.B.Ch.B
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2014 Volume: 10 Issue: 1 Pages: 121-125
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chronic otitis media (COM) of mucosal or squamous type is a common problem in otolaryngology practice, the active form of COM is characterized by discharge of pus and is treated by antibiotics to start with, the appropriate antibiotic should be prescribed to avoid antibiotic abuse and guarantee good outcome. Objectives:The objective of this study is to identify the causative organisms of active chronic active otitis media both (mucosal, squamous) type and test their sensitivity to various anti- microbial agents &compare with abroad studies.Methods:A prospective study was done on eighty patients, different ages and sexes were taken and carful history and examination was done, examination under microscope was done with carful suction to ensure absence of cholesteatoma, ear swabs were collected from middle ear discharge, bacteria identified by gram-staining and biochemical tests and antibiotic sensitivity were tested by using disc-perfusion method.Results:The culture results of eighty patients with chronic active otitis media, showed Pseudomonas aeroginosa from 26 patients (32.5%) and Proteus species from 18 patients (22.5%) and Staphylococcus aureus from 12 patients (15%), Providentia from 8 patients (10%) and Sarretia from 6 patients. (7.5%), mixed gram–ve bacteria isolated from 7.5% of patients, Klebsiella Ozaenae from 5%. of patients. no anaerobic bacteria were isolated in this study (table 1, 2).Antibiotic susceptibility showed sensitivity of Ps. Aeroginosa to menepem, third generation cephalosporin, ciprofluxacillin and resistance to gentamicin. Klebsiella Ozaenae showed resistant to many antibiotic also Serratia as shown in table 3. Conclusions:the microbial study of middle ear discharge is very important because the medical treatment is still a main part in treatment of chronic active otitis media so identifying the type of microorganisms and its sensitivity to antibiotics is give a good chance to successful control of the infection.

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