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Article
Viability, survivability and tolerability of Rhodococcus pyridinivorans GM3 for phenol concentrations

Authors: Mahammed E. Jabbar Al-Defiery --- Gopal Reddy
Journal: Mesopotamia Environmental Journal بيئة وادي الرافدين ISSN: 24102598 Year: 2015 Volume: 1 Issue: 3 Pages: 57-65
Publisher: Babylon University جامعة بابل

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Abstract

Microorganisms play a major role in saving our environment by degrading xenobiotic compounds and chemical wastes, which are toxic either in their native or modified form. Bacteria capable of degrading phenol are common and comprise aerobic and anaerobic catabolizing phenol as a sole source of energy and carbon.The bacterial isolate Rhodococcus pyridinivorans GM3 was studied for viability, survivability and tolerability GM3 for different concentrations of phenol in mineral salts medium(MSM). The results showed that R. pyridinivorans GM3 had powerful adaptability to the some conditions of environment, which exhibited that it has a high survivability and tolerability upto 7.0 g/L of phenol concentration in the medium. The tolerance of microorganism to phenol toxicity reduced with increasing concentrations. There was increase in survivability of R. pyridinivorans GM3 on MSM when inoculum size was increased from 1% to 4%.The results suggest that inoculum size led to increase survivability and tolerability. The results of this study clearly demonstrate that presence of phenol in media upto 2.0 g/L does not adversely affect the viability. However, the inhibitory effect of phenol on viability indicated that GM3 could not degrade 2.5 g/L phenol concentration with 1% inoculum size. R. pyridinivoransGM3 exhibited highest potential forphenol concentrations.


Article
عزل وتشخيص بكتريا مكسرة للفينول من ترب محلية في محافظة بابل

Author: فرح طارق عبد الرضا
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2015 Volume: 23 Issue: 1 Pages: 136-143
Publisher: Babylon University جامعة بابل

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Abstract

Eight bacterial isolated of phenol biodegrading were isolated from agricultural and oily polluted soils. These isolates were identified according to cultural features and biochemical tests. The results showed that five isolates were belonged to Bacillus subtilis while two isolates belonged to P. aernginosa from agricultural soils and one isolate form oily polluted soil. The ability of these isolate to tolerant phenol concentration from 10-100mg/L. All of these isolates were tolerant to these concentration but the concentration was studied 20mg/L was the most prefer concentration due to the absorbance on 600nm for 84hr old bacterial culture at 37co.

عزلت (8) عزلات بكتيرية مكسرة للفينول من الترب الزراعية والترب الملوثة بمخلفات زيوت محركات الديزل من محطة محلية لتوليد الطاقة الكهربائية في محافظة بابل وقد شخصت هذه العزلات اعتماداً على الصفات الزرعية والفحوصات البايوكيمياوية. اظهرت النتائج ان خمسة منها تعود الى B. subtilis وعزلتين تعود الى P. aeruginosa من الترب الزراعية وعزلة واحدة من الترب الملوثة بمخلفات زيوت الديزل تعود الى P. aeruginosa. درست قابلية هذه العزلات على تحمل التراكيز المختلفة من الفينول 10-100 ملغم /لتر وقد نمت جميع العزلات على هذه التراكيز الا ان التركيز 20 ملغم/لتر كان الامثل لنموها بدلالة قياس الامتصاصية على طول موجي 600nm للمزروع البكتيري بعد 48 ساعة من الحضانة عند درجة حرارة 37o.


Article
Antifungal Activity of Alkaloids and Phenols Compounds extracted from black pepper Piper nigrum against some pathogenic fungi
فعالية مركبات القلويدات والفينولات المستخلصه من الفلفل الأسود ضد بعض الفطريات الممرضه

Author: Batol Imran Dheeb بتول عمران ذيب
Journal: Jornal of Biotechnology Research Center مجلة مركز بحوث التقنيات الاحيائية ISSN: 18151140 Year: 2015 Volume: 9 Issue: 2 Pages: 46-54
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

This study focused on the production of alkaloids and phenols from dry black pepper Piper nigrum fruit; and its effect on thirteen species of fungi isolated from dermal infection. The antifungal activity of different concentrations alkaloids and phenols were evaluated using agar dilution method. Statistics revealed different inhibition percentages; that are gradually increased with the increasing in the concentration of the used compound. Minimal inhibitory concentration MIC and maximum fungicidal concentration MFC were obtained 0.4, 0.002 mg/ml respectively. Confirming the presence of alkaloids and phenols were done by fast liquid chromatography; showed the presence of two types of alkaloids and ten types of phenols.

ركزت هذه الدراسه على انتاج القلويدات والفينولات من ثمار الفلفل الأسود ودراسة تأثيرها على 13 نوع من الفطريات المعزوله من اصابات جلديه، قُيمت فعالية القلويدات والفينولات المحضرة بتراكيز مختلفه من خلال طريقة المزج بالأكار و أظهرت نسب تثبيط مختلفه تزداد مع زيادة تركيز المركب المستخدم بعد تحليل النتائج احصائيا.تم الحصول على قيمة التركيز المثبط الأدنى والتركيز الأعلى القاتل للممرضات المستخدمه في التجربه وكان 0.002, 0.4ملغم/ مل على التوالي تم التحليل والكشف عن القلويدات والفينولات باستخدام الكرموتوغرافيا السريعه السائلة وأظهر التحليل وجود نوعين من القلويدات و عشرة أنواع من الفينولات في المستخلص الذي تم تحضيره والكشف عنه.

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