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Molecular Characterization of Salmonella Typhi Isolated from Typhoidial Humans

Authors: Adil Abead Hassuny --- Iman Jawad Kadhim --- Akbal Harby Kadhim
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 646-652
Publisher: Babylon University جامعة بابل

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Abstract

Present research work was carried out for detection and molecular characterization of Salmonella Typhi isolated from Blood plasma of person severing from Typhoid fever using biochemical test, and detection of virulence gene by using of polymerase chain reaction (PCR) techniques. A total of 12 clinical samples were collected from patients. The isolated strains were also investigated for antibiotic susceptibility patterns as a control measure. Microbiological and biochemical investigations revealed the presence of Salmonella typhi from 12 samples. PCR analysis confirmed these presence of fliC (Phage-1 flagellin gene), viaB (Vi antigen gene) and SipA genes. Antibiotic susceptibility test was carried out among the isolates against 12 antimicrobial agents. Results showed that 100 % resistance to only ampicillin and 100 % sensitivity to carbenicillin, chloramphenicol, clindamycin, gentamycin, kanamycin, and tetracycline.

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