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Article
STUDY OF SCENEDESMUS ALGAE GROWTH IN A SPLITCOLUM AIR-LIFT PHOTOBIOREACTOR
دراسة نمو طحالب Scenedesmus باستخدام مفاعل بيو ضوئي ذو الرفع الهوائيالانقسامي

Author: Amer D. Zmat Albdiri
Journal: Al-Qadisiyah Journal for Engineering Sciences مجلة القادسية للعلوم الهندسية ISSN: 19984456 Year: 2015 Volume: 8 Issue: 3 Pages: 410-421
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

The growth of Scenedesmus algae, cultivated in a split-column airlift bioreactor under threedifferent light intensities (94, 187, 468 E /m .s 2  ) and two values of superficial gas velocities (0.3,1 cm/s), was investigated. The physical properties of the culture medium were monitored byvarious analytical methods (optical density, chlorophyll concentration). Samples were taken twice aday (10:00 AM and 5:00 PM) for cell count and optical density measurements. The dynamicgrowth rate of the algae was studied using the integrated three-state fluid dynamics modeldeveloped by Eilers and Peeters (1988). The kinetic parameters for the system under definedlight/dark cycles were evaluated. The obtained results indicate that the growth rate and chlorophyllcontent of the Scenedesmus algae directly proportional to light intensity and superficial gas velocity.

تم دراسة نمو طحالباستخدام مفاعل بيو ضوئي من نوع عمود الرفع الهوائي الانقسامي تحت Scenedesmus0.3 ). تم رصد , 1 cm/s), 94 ) وسرع غاز سطحية , 187, 468 E /m2 .s ) ظروف مختلفة من الشدة الضوئيةوتقييم الخصائص الفيزيائية للوسط باستخدام طرق تحليلية مختلفة )الكثافة الضوئية وتركيز الكلوروفيل(. تؤخذ عيناتالفحص مرتين يوميا ) 01:11 صباحا و 0:11 مساء( لأغراض قياسات الكثافة الضوئية وعد الخلايا. تم دراسة معدلباستخدام موديل ديناميكية الاطوار الثلاثة التكاملي الذي طور بواسطة الباحثين Scenedesmus النمو الحيوي لطحالبيتناسب طرديا مع الكثافة Scenedesmus اظهرت النتائج ان معدل النمو لطحالب .Eilers and Peeters (1988)الضوئية لمصدر الاضاءة وسرعة الغاز السطحية.


Article
Evaluation the effect of hyaluronic acid on bone healing process in rabbits (Immunohistochemical study for TGF-β)

Authors: Ibtihal H. Radhi --- Nada M. Al-Ghaban
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 1 Pages: 111-116
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Bone augmentation techniques are commonly employed in medical fields. This biomaterial system mustbe readily available, easily applicable by minimally-invasive technique and able to release an osteoinductivegrowth factor. Such a system will be able to engineer new bone formation locally at the site of injection. Hyaluronicacid has osteogenic potential that can be exploited not only for repairing bone defects but also for providingtransplantable bone for the reconstruction of a variety of bone defects. The aims of this study were to evaluate theeffects of Hyaluronic acid gel on bone healing by immunohistochemical estimation of transforming growth factor -beta 3 in experimental and control groups.Materials and methods: Thirty two New Zealand male rabbits were used in this study .Two Intra bony holes were madefor each rabbits on the right and left buccal side of the upper diastema. The right hole was filled with Hyaluronic acidgel (experimental one), while the left hole was left for normal healing (control one). The rabbits were randomlydivided and sacrificed at 1, 2, 3 and 6 weeks post operatively. Immunohistochemical test for the expression of TGF-β3were performed on bone specimens of both control and experimental groups at all healing interval.Results: Immunohistochemical examination of this study revealed that the hyaluronic acid treatment increased thepositive expression of TGF-β3by osteoblasts, osteocytes and bone marrow stromal cells especially in 1 and 2 weeksintervals than that observed in control one.Conclusions: The present study illustrated that the Hyaluronic acid was osteoconductive material that enhanceosteogenesis and accelerated the bone healing process


Article
Efficacy of Methionine supplementation on the Growth Performance and Lymphoid organs indices of Broiler Chickens Vaccinated with combined Infectious Bronchitis Newcastle disease Vaccines
فاعلية اضافة المثيونين على اداء النمو ومؤشرات الاعضاء اللمفاوية في دجاج اللحم الملقح معا بلقاحي التهاب القصبات المعدي والنيوكاسل

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Abstract

Methionine (Met.) is amino acid that has proven immune regulatory action. It is usually first limiting amino acid in maize and soybean meal based diet. Met. supplementation in growing chick is a common practice (Swick et al., 1990; Shini & Brydeen, 2005). One of the mechanisms proposed to explain Met. interference in the immune system is the proliferation of T cells, which are sensitive to intracellular glutathione and cysteine levels, compounds also participate in Met. metabolism (Kinscherf et al., 1994). The aim of this study was to investigate the efficacy of Methionine (Met) supplementation on the growth performance and lymphoid organs indices of broiler chickens vaccinated with combined infectious bronchitis (IB)Newcastle disease (ND) vaccines. A total of 120 newly hatched commercial broiler chicks were assigned into four equal treatment groups as follow: First group chicks were vaccinated against IB and ND at 8 and 21 days of age without Met. Second group chicks were vaccinated against IB and ND at 8 and 21 days of age with 0.5% Met. in the diet. Third group chicks remain as control group. Fourth group chicks remain as control group with 0.5% Met. in the diet. Body weight (BW), body weight gain (BWG), feed intake (FI) and lymphoid organs indices were estimated in all groups. Statistical analysis revealed significant (P<0.05) differences among the treatment groups in BW, BWG and better FI as compared with non treatment group. The mean value of thymus, bursa of Fabricius and spleen index of chicks supplemented Met during the period of age showed significant increment (P<0.05). We concluded that better growth performance and lymphoid indices could be obtained with adequate supplementation of Met which have been identified to be in marginal quantities in poultry feed.

ميثيونين حوامض أمينية التي يعمل في تنظيم للمناعة. هي حوامض أمينية محددة أولية عادة موجودة في الذرة الصفراء والصويا كوجبة طعام في العليقة. أضافة الميثيونين في عليقة الافراخ النامية شائع. أحدى الأليات المقترحة لتوضيح تدخل الميثيونين في نظام المناعة هو انتشار خلايا T, الذي حساس لمستويات الكليوتاثيون والسيستين, تشارك المركبات أيضا في أيض الميثيونين. ان هذه الدراسة تهدف إلى التعرف على فعالية اضافة الميثيونين على أداء النمو ومعدل وزن غدة التوثة, جراب فابريشا والطحال إلى وزن الجسم في الدجاج اللاحم الملقحة بلقاحي التهاب الشعب الهوائية المعدية ونيوكاسل. استعمل في هذه الدراسة 120 من افراخ الدجاج اللاحم التجارية بعمر يوم واحد قسمت هذه الافراخ إلى أربع مجموعات متساوية على النحو التالي في المعاملة: المجموعة الاولى ( (G1تم تحصين الافراخ ضد التهاب الشعب الهوائية المعدية ونيوكاسل في 8 و 21 يوما من العمر دون اضافة الميثيونين. المجموعة الثانية (G2) تم تحصين الافراخ ضد التهاب الشعب الهوائية المعدية ونيوكاسل في 8 و 21 يوما من العمر مع إضافة 0.5٪ من الميثيونين الى العليقة. المجموعة الثالثة (G3) عدت كمجموعة سيطرة. اما المجموعة الرابعة (G4) عدت كمجموعة سيطرة ولكن باضافة الميثيونين 0.5٪ الى العليقة.تم جمع البيانات من زيادة الوزن، الزيادة في وزن الجسم واستهلاك العلف من عمر (0-35) يوم. وكشف التحليل الإحصائي للبيانات وجود فروق معنوية(P<0.05) بين مجموعات المعالجة في الوزن وبالزيادة الوزنية. على اية حال، اظهر النتائج وجود زيادة بالوزن وبالزيادة الوزنية وزيادة استهلاك العلف في المجموعتين المضافة اليها المثيونين. وأظهر زيادة معدل قيم غدة التوثة, جراب فابريشا والطحال في الافراخ المضافة اليها الميثيونين خلال فترة التربية فروق معنوية .(P <0.05) خلصنا إلى أن أفضل أداء النمو ومؤشرات اللمفاوية يمكن الحصول عليها مع اضافة كافية من المثيونين التي تم تحديدها لتكون في الكميات الهامشية في أعلاف الدواجن


Article
Impact of Human Serum on Pharmacodynamic Action of Echinocandins against Aspergillus spp. : an in Vitro Study

Author: Hussam Wahab Al-Humadi
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 603-611
Publisher: Babylon University جامعة بابل

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Abstract

Echinocandins represent a class of lipopeptide antifungal agents that possess a high protein binding capacity which could have an impact on their activity. This study was focusing on the role of human serum on the activities of echinocannins (caspofungin, micafungin, and anidulafungin) against Aspergillus species (A.fumigatus, A.flavus and A.terreus). The aspergillus growths have been assessed by measuring minimal effective concentrations of each (caspofungin (0.5-1 mg/L), micafungin (0.06-0.12 mg/L) and anidulafungin (0.03-0.09 mg/L). Broth microdilution method was used to study in vitro activities based on the Clinical and Laboratory Standards Institute documentation (CLSI M38-A2) by using standard medium (Roswell Park Memorial Institute (RPMI 1640)) with or without 50% human serum. Each drug was diluted ranging from 0.03-4 mg/L, inoculated with 2x104 Colony Forming Units/ml for each isolate, then incubated at 37οC for 10 hrs to A.fumigatus and A.flavus and 15 hrs to A.terreus. The fungal growth was assessed by measuring the galactomannan index for each concentration with ELISA (Platelia Aspergillus); percentage of fungal growth inhibition was measured from galactomannan index and analyzed as maximal growth inhibition (Emax) and drug concentration with 50% of growth inhibition (IC50). IC50 values of echinocandins were ranged from 0.03-0.06 mg/L without serum and 0.09-0.25 mg/L in presence of serum while Εmax values were 50(44-56)% for A.fumigatus, 70(65-74)% for A.flavus and 65(63-66)% for A.terreus without serum and 94(92-96)% for A.fumigatus, 92(91-95)% for A.flavus and 80(75-83)% for A.terreus with human serum. The study concluded the effective concentrations of all echinocandins were increased concomitantly with more than 90% fungal growth inhibition with human serum.


Article
Normal and Abnormal Variations of Sella Turcica in Three Facial Types of Adolescent Iraqi Samples

Authors: Issam Merzah Abdullah --- Lamis Khidher Mohammed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 653-660
Publisher: Babylon University جامعة بابل

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Abstract

Sella(S) turcica is a structure resemble a saddle and based on the roof of the sphenoid bone. For cephalometric tracing, sella point is one of the most commonly used cranial landmarks and it is located in the centre of the sella turcica of the skull. This study were to assess the shape and size of sella in a sample of Iraqi adolescent and with different skeletal classes. The study sample consist of (91) Iraqi adolescent subjects aged 10-16 years (47 females, 44 males); every subject provided with true lateral cephalometric radiograph. The sample was classified into three skeletal classes according to ANB angle. The size of Sella was measured using three linear measurements (S. length, S. depth, and S. diameter). It showed that most of the measurements of sella turcica were not different statistically among the skeletal classes. Normal sella was the predominant over the other morphological aberrations, while these morphologies occurred more frequently in class II and III. throughout the pubertal period specific sella turcica linear measurements cannot be obtained for each specific skeletal class; except between class I and II patterns, Sella depth was statistically higher in the former.


Article
Effect of Magnetic Field on Peroxidase Activity and Growth of Panicum miliaceum L. Seeds

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Abstract

In latest years in agricultural sector, physical techniques based on magnetic field are being designed. The present work studied the impact of magnetic field on the germination of fresh and partially-aged Panicum miliaceum L. (PM) seeds. The germinated PM seeds were categorized into 6 groups. Groups 1,2 and 3 were fresh seeds, and Groups 4, 5 and 6 (LV30%) were left for 6 days to lose about 30% of their viabilityLV30% seeds. Groups 1 and 4 were normal unexposed seeds. Groups 2 and 5 were seeds exposed to magnetic field intensity of 100mT, while those of 3 and 6 to 200mT for 60 minutes. Germination was monitored on days 2, 5, 7, 10, 12, 14 and 16. The effect of magnetic field on the % germination, growth characteristics of radical and shoot, protein concentration and peroxidase activity in fresh and LV30%were studied. Both the exposed and unexposed seeds lost their viability by 16 days of ageing in fresh and 14 days in (LV30%). Exposed seeds to magnetic fields 100 and 200 mT revealed significant increase in the length of root and shoot compared to normal unexposed seeds. The magnetically exposed aged seeds had significantly higher protein than in the unexposed aged seeds. The peroxidase activity decreased with ageing and the magnetically exposed seeds showed higher activity than the corresponding aged unexposed normal seeds. The present study suggests the magnetic field could accelerate the germination of PM seeds, accelerate their growth characteristics and increase soluble protein content. In addition the peroxidase activity significantly improved.


Article
Study of Ossification Stages in Quail Embryo (Coturnex japonica)

Author: Hashim M.A-Kareem
Journal: journal of al-qadisiyah for pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2015 Volume: 2 Issue: 20 Pages: 44-52
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

A morphogenesis study has been made for the skeleton formation of the quail(Coturnex japonica ) which included the analytical study to make the skeletal elements andthe transitional changes that happens in the two processes of cartilage formation(Chondrofication ) and bones (Ossification) during the growth of the different natural phasesfor the diverse bones of the body.A quail was gathered of about (100) birds and brought up at home. Then the fertilizedegg was gathered from those birds in about (7-9) days, those egg were incubated by anUkranian electronic incubator in a about ( 37,5-38 Co) and 70% of temperature and humidityrespectively. The embryos were used starting from day 3 of incubation to day 16 ofincubation , which is the hatching day.Embryos were extracted from the egg starting from day 3 of incubation,they weretreated with alcian blue pigment that is related to paint the (non ossified) cartilaginous partsof bones, and alizarin red – s pigment that is related to paint the ossified parts of the bones asan indicator for calcium in order to recognize the ossified parts from those that didn't ossifiedfor each bone of the skeletal system of the quail.The method that used to treat the embryoswith the two pigments differed with the age progress of the embryo , also it's concentrationincreased with the progress of the embryos age due to the formation of the organs, skin andmuscles as well as the strengthening of the cartilage and it's transformation into solid bonesday after day.The successive phases for the growth of the different bones were traced with completeconcentration on the evolution of the cartilage (Chondrofication) and it's transitionaltransformation into bone (Ossification) for each bone of the vertebral skeleton , thorax gage ,and the fore and hind limbs bones.


Article
The role of topical application of bone morphogenetic protein 7 (BMP7) on bone healing on rabbits (Immunohistochemical study on TGF-β 3 & IGF-1R)

Authors: Rabab Ikram Ghareeb رباب اكرام غريب --- Eman Issa Al-Tamemi ايمان عيسى التميمي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 3 Pages: 70-78
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Bone is essentially a highly vascular, living, constantly changing mineralized connective tissue. It isremarkable for its hardness, resilience and regenerative capacity, as well as its characteristic growth mechanisms.This study aimed to:1. To evaluate the effect of bone morphogenetic protein7 (BMP7) on bone healing in artificially created intrabonydefect in rabbits upper diastema, histologically.2. To study the immunohistochemical expression of TGF-β3 and IGF-1R as bone formation markers in experimentaland control groups during bone healing.Material and method: Forty male rabbits, was used in this study, 8 rabbits for each healing interval (3 days, 1,2 ,4 and6 weeks). In each rabbit two bone holes were created on the right and left sides of the maxilla.BMP7 was applied tothe bone hole in the left side while bone hole in the right left for normal healing. Routine processing and sectioningtechnique performed for histological evaluation. Immunohistochemical analysis utilized to localize the expression ofTGF-β3 and IGF-1R in experimental and control groups for all animals.Results: Histological findings indicated that bone defect coated with BMP7 illustrated an early bone formation,mineralization and maturation in comparison to control group. Immunohistochemical findings revealed high positiveexpression for TGF-B3 and IGF-1R in experimental in comparison to control group.Conclusion: The study concluded that BMP7 protein enhance bone healing and maturation, also it regulate theexpression of TGF-B3 and IGF1R in bone.


Article
Effect of Commercial Dry Yeast Saccharomyces cerevisiae on Growth Performance in Common Carp Cyprinus carpio L.
تأثير الخميرة الجافة التجارية Saccharomyces cerevisiae في أداء نمو اسماك الكارب الشائع Cyprinus carpio L.

Authors: Nasreen Mohi Alddin Abdulrahman نسرين محي الدين عبدالرحمن --- Firas Abdul Malik Al-Jader فراس عبدالمالك الجادر
Journal: Iraqi Journal of Science and Technology المجلة العراقية للعلوم والتكنولوجيا ISSN: 18136893 Year: 2015 Volume: 6 Issue: 2 Pages: 110-117
Publisher: Ministry of science and technology وزارة العلوم والتكنولوجيا

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Abstract

This study was designed to investigate the effect of adding different level of commercial dry yeast Saccharomyces cerevisiae in three different concentrations with three treatments T1 (0%), T2 (3%), and T3 (7%) on growth performance of common carp Cyprinus carpio L., the trail was conducted for 56 days and by using 200 fingerlings common carp with mean initial weight (46g). Fish were acclimated to concrete ponds belongs to department of Animal Sources/ College of Agriculture/ University of Duhok. Fish were fed with control pellets (31% protein) prior to the feeding trials for 21 days. There was significant differences in the final weight attained by common carp at all levels of dry yeast incorporation as compared to the control diet. However, the addition of 7% dry yeast resulted in significantly increase in fish specific and relative growth rate.

صممت التجربة لبيان تأثير إضافة تراكيز مختلفة من الخميرة الجاهزة التجارية بثلاث تراكيز الى علائق الاسماك وكما يلي المعاملة الاولى T1 ( 0%) والمعاملة الثانية T2 (3%) والمعاملة الثالثة T3 (7%) في بعض عناصر الاداء الانتاجي لاسماك الكارب الشائع Cyprinus carpio L. استمرت التجربة لمدة 56 يوما، باستخدام 200 اصبعية بمعدل وزن ( 46 غم/ سمكة). تم اقلمة الاسماك لظروف التربية في احواض كونكريتية تابعة الى قسم الثروة الحيوانية/كلية الزراعة والغابات/ جامعة دهوك. وتم تغذيتها بعليقة تجارية ( نسبة البروتين 31%) قبل التجربة ولمدة 21 يوما. لوحظت إختلافات معنوية في الوزن النهائي للاسماك في تركيزي الخميرة المستخدمة، ادى استخدام الخميرة بتركيز 7% الى زيادة معنوية في النمو في معدل النمو النوعي والنسبي لاسماك التجربة.


Article
Serum Vascular Endothelial Growth Factor Levels in Iraqi Patients with Newly Diagnosed Acute Leukemia

Authors: Abass F. Hasson --- Najlaa B. Alawadi --- Saba S. Mallouh
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 1 Pages: 8-16
Publisher: Babylon University جامعة بابل

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Abstract

Acute leukemia is an aggressive disorder which need a new therapeutic approaches to improve the response to treatment. Angiogenesis seems to be important both for leukemogenesis and susceptibility to intensive chemotherapy, and antiangiogenic strategies may therefore be considered for the treatment of acute leukemia.This study aim to assess serum VEGF levels in Iraqi patients with a newly diagnosed acute leukemias and to find the relation between VEGF levels and other hematological parameters. This case-control study included 96 subjects who attended Marjan Teaching Hospital in Babil/Iraq during the period from Nov 2013 to Aug 2014.They divided into two groups; patient group included 48 patients newly diagnosed with acute leukemia (32 of them had AML and 16 had ALL) and control group included 48 normal volunteers. Complete blood count and serum VEGF (ELISA) were done for all while bone marrow examination was done for patients only. Mean serum VEGF was significantly higher in patients than controls (643.8 ±121.1 ng/dl vs. 203.4 ±51.8 ng/dl respectively and P-value < 0.001) and a higher levels were found in patients with ALL compared to AML and in males compared to females. No statistically significant age differences were found between patients and controls, while majority of patients (61.9%) and controls (63.4%) were males. Mean PCV was significantly lower in patients than controls (P-value < 0.01), Mean WBC count was significantly higher in patients than controls (P-value < 0.01) and mean platelets count was significantly lower in patients than controls (P-value < 0.01).This study concluded that serum VEGF in patients with acute leukemia was high compared to controls and a significant correlation was found between serum VEGF levels and both PCV and gender.

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