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Co-localization of EBV and Expression of P16- Cyclin-Dependent Kinase inhibitor Protein in Tissues from Patients with Non- Hodgkin's Lymphoma

Authors: Shakir H. Mohammed Al-Alwany --- Saad Hasan Mohammed Ali --- Masar Riyadh Rashid AL-Mousawi
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2016 Volume: 9 Issue: 1 Pages: 2386-2396
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: EBV has been classified as a group 1 carcinogen associated with a variety of lymphoid and epithelial cancers. EBV was evidenced as a monogenic virus from its ability to transform normal human B cells, resulting in immortalization of the infected cells. A tumor suppressor protein, P16, is a cyclin-dependent kinase inhibitor plays a critical molecular role in cell senescence, regulation of the apoptosis pathway and G1 cell cycle arrest.Aim of the study: To analyze the concordant frequency and impact of P16 protein expression and EBV infection on tissues from a group of patients with Non- Hodgkin's Lymphoma (NHL).Patients and methods: Forty-five (45) formalin-fixed, paraffin- embedded tissues from lymph nodes biopsies were enrolled in this study; (30) lymph nodes biopsies were related to patients with NHL and (15) lymph nodes autopsies have included as apparently normal control group. Detection of EBV was done by ultra-sensitive version of in situ hybridization (ISH) method whereas immunohistochemical (IHC) system was used to demonstrate the protein expression of P16 tumor suppressor gene.Results: Detection of EBV -ISH reactions in tissues with NHL was observed in 17 out of 30 (56.7%), while in the tissues from lymph nodes autopsies was detected in 6.7% (1 out of 15).Positive P16- IHC reactions were observed in 14 out of 30 NHL cases (47.1%). No P16 positive – IHC reaction was detected in healthy lymph node tissues in the control group. The differences between the percentages of EBV and P16 detection in NHL tissues and control tissues group were statistically highly significant (P value = < 0.0001). Conclusions: The significant protein expression of P16 tumor suppressor gene as well as EBV infection in NHL in our results could indicate that cell cycle dysregulation and EBV-related transformation are important events in the pathogenesis of subset of NHL.


Article
Immunohistochemical Localization of Epstein Barr Virus- Latent Membrane Protein 1 in Breast Cancer Tissues

Author: Saad Hasan Mohammed Ali *,Shakir H. Mohammed Al-Alwany**, Ghanim I.A. Al-Wadi *
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 2 Pages: 234-240
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACK GROUND:Epstein- Barr virus (EBV) is well known to infect the vast majority of the world's adult population and has recently been reported in many literatures for its association with a broad spectrum of benign and malignant tumors, including breast cancer. OBJECTIVE: To analyze the relations and impact of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) expression on a group of tissues with primary invasive breast cancers.PATIENTS AND METHODS: Forty- nine (49) formalin-fixed, paraffin- embedded breast tissues were obtained in this study; (34) biopsies from breast cancers (BC) and (15) from apparently normal breast autopsies control group. Detection of expression of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) was done by HRP/DAB immunohistochemistry detection kit (an immunoenzymatic antigen detection system for immunohistochemistry techniques) using specific primary antibodies for EBV-LMP 1.RESULTS: Detection of EBV-LMP 1 - immunohistochemical (IHC) reactions in tissues with BC was observed in 11 out of 34 (32.4%), while in healthy breast tissues in the control group was detected in 13.3% (2 out of 15). The difference between the percentages of EBV-LMP 1 detection in BC tissues & control group was statistically not significant (P >0.05).While no significant differences were observed between EBV-LMP 1 detection in BC tissues & the age of patients as well as grade of invasive breast cancers (P value > 0.05).CONCLUSION: Our results indicate that EBV could have a role in the pathogenesis of subset of breast cancers and contribution in their carcinogenesis. .

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