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Article
Effect of phosphorus concentration and light intensity on protein content of microalga Chlorella vulgaris

Authors: Maysam Alaa Baiee --- Jasim M. Salman
Journal: Mesopotamia Environmental Journal بيئة وادي الرافدين ISSN: 24102598 Year: 2016 Volume: 2 Issue: 2 Pages: 75-86
Publisher: Babylon University جامعة بابل

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Abstract

The exploitation of microalgae as a protein source has led to increased interest in the use of microalgae in health food production and source of animal feed. Three light intensities (125, 268 and 300 μEm2sec) and four phosphorous concentrations (0, 2, 4, 6, gl) were used to study their effect on the growth and amount of protein content in Chlorella vulgaris alga. The growth curve of the studied alga was different among the treatments. The stationary phase began at the days 13, 12 and 9 for treatments 125, 268 and 300 (μEm2sec) respectively, while it was identified as day 14, 12, 10 and 6 in treatments 6, 4, 2 and 0 gl of phosphorous respectively. The highest K value was 0.15 at the 268 μEm2sec (control), while the lowest K value was 0.08 at 125 μEm2sec. The shortest doubling time (G) was 2 days at control (268 μEm2sec) treatment.When used different phosphorous concentrations, The highest K value was 0.16 at 0 gl treatment, while the lower K value was 0.1 at 6 gl treatment. The shortest doubling time (G) was 1.8 day in treatment 0 gl.Protein content of C. vulgaris increased from 39.46% to 57.51% at 125 μEm2sec to 300 μEm2sec light intensity. When used different phosphorus concentrations, protein content increased from 51.17% at control treatment (4 gl) to 75.56% at 6 gl treatment.


Article
Cultivation of Microalgae Chlorella vulgaris in Airlift photobioreactor for Biomass Production using commercial NPK Nutrients
استنبات الطحالب الدقيقة Chlorella vulgaris في مفاعل الرفع الهوائي الحيوي الضوئي لانتاج الكتلة الحيوية بأستخدام سماد NPK التجاري

Author: Saad Hanash Ammar سعد حنش عمار
Journal: Al-Khwarizmi Engineering Journal مجلة الخوارزمي الهندسية ISSN: 18181171 23120789 Year: 2016 Volume: 12 Issue: 1 Pages: 90-99
Publisher: Baghdad University جامعة بغداد

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Abstract

Airlift reactors are widely used in the chemical and biochemical applications as effective contactors for mass and heat transfer. The main advantages of airlift contactor compared with simple bubble column are ease of construction, low shear rate, high capacity, good mixing and liquid circulation without mechanical agitators and circulating pumps.In this work, growth characteristics of Chlorella vulgaris microalgae were studied in an internal loop airlift photobioreactor for biomass production. The bioreactor operated under batch and semi-continuous culture mode using commercially available 20:20:20+TE NPK fertilizer as nutrients. The experiments were conducted to evaluate the growth rate and biomass productivity of Chlorella vulgaris microalgae as affected by several factors such as nutrients concentration (20-80 mg/L), inlet air flow rate (2-8 LPM), and harvesting ratio (10-30 vol.%). The growth rate and biomass productivity of Chlorella vulgaris was determined as changes in optical density using UV-spectrophotometer. The results of batch operation showed that the growth rate of Chlorella vulgaris microalgae was increase with increasing of NPK nutrient concentration used but the access to the stationary phase of growth was delayed. The rate of growth was also increase with the increase in air flowrate to a limit then decrease. On the other hand the airlift photobioreactor can be operated in semi- continuous mode successfully by choosing the optimum conditions from the batch step which was 40 mg/L NPK nutrients concentration and 6 LPM and air flowrate. Several ratios of reactor content were harvested and the maximum biomass productivity was 0.142 g/L.day when harvested 10 vol.% every two days.

في هذا العمل، تم دراسة خصائص النمو للطحالب الدقيقةChlorella vulgaris في مفاعل الرفع الهوائي الحيوي الضوئي ذي التدويرالداخلي لإنتاج الكتلة الحيوية. المفاعل الحيوي المستخدم يعمل تحت نظامين هما النظام الدفعي والنظام شبه المستمر وباستخدام مغذيات واطئة الكلفة ومتاحة تجاريا وهي سماد الـ NPK 20:20:20+TE. أجريت التجارب لتقويم معدل النمو و إنتاجية الكتلة الحيوية للطحالب وتأثرهما بعدة عوامل مثل تركيز المغذيات (20-80 ملغ / لتر)، ومعدل تدفق الهواء الداخل (2-8 لتر / دقيقة)، ونسبة الحصاد (10 -30 ٪ حجما).وأظهرت النتائج العملية للنظام الدفعي أن معدل نمو الطحالب الدقيقة Chlorella vulgaris يزداد مع زيادة تركيز المغذيات المستخدمة ولكن الوصول إلى مرحلة ثابتة من النمو يتأخر كذلك يزداد معدل النمو بزيادة معدل جريان الهواء في المفاعل الحيوي الى حد معين بعدها ينخفض. من ناحية أخرى يمكن تشغيل مفاعل الرفع الهوائي الحيوي الضوئي في وضع شبه المستمر بنجاح وذلك باختيار وتطبيق افضل الضروف المستحصلة من عملية النظام الدفعي من تركيز المغذيات و معدل جريان الهواء والتي كانت 40 ملغم / لتر و 6 لتر/ دقيقة على التوالي تم حصاد عدة نسب من محتوى المفاعل وان أقصى قدر من انتاجية الكتلة الحيوية المستحصلة كانت 0.142 (غرام / لتر.يوم) عند نسبة حصاد 10 ٪ حجما كل يومين.

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