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Article
Pharmacological modulation of dendritic cell function by anti-inflammatory dexamethasone but not by GSH depletion

Author: Bassim I Mohammad
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2016 Volume: 15 Issue: 2 Pages: 60-68
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

The principal function of dendritic cells (DCs) is to acquire antigens from the environment and present them to naïve T-cells to initiate an immune response. DCs also provide potent co-stimulatory signals to T-cells through expression of co-stimulatory receptors such as CD40 and CD86. Anti-inflammatory and other drugs that deplete intracellular glutathione (GSH) can affect the immune response. However, it is unclear whether these drugs can modulate DC function. In this study, we evaluated the effects of the anti-inflammatory drug dexamethasone and the GSH lowering compound as buthionine sulfoximine (BSO) on DC function. Mouse bone marrow derived dendritic cells (BMDCs) were generated and treated with dexamethasone or BSO. Expression of the co-stimulatory molecules MHCII, CD86 and CD40 was quantified by flow cytometry. Endocytic and phagocytic capacity of DCs was measured by dextran uptake and necrotic cell phagocytosis respectively. We found that dexamethasone reduces the expression of co-stimulatory molecules both in immature and mature DCs. Dexamethasone had a marginal increase in the endocytic capacity of iDCs but caused a decrease in phagocytic function. Reducing GSH levels did not affect co-stimulatory molecules expression, endocytic and phagocytic capacity of iDCs. We conclude that anti-inflammatory corticosteroids could modulate the immune response by down regulating DC co-stimulatory molecule and by inhibition of phagocytosis. Further work is needed to examine the ability of dexamethasone treated DCs to stimulate T-cell activation. These findings have


Article
STUDY THE ANTIOXIDANT EFFECT OF TOMATO EXTRACT IN OXIDATIVE STRESSED RATS.
دراسة التأثير المضاد للتاكسد لمستخلص الطماطم ضد الإجهاد ألتأكسدي في الجرذان

Author: Haider H. Humaish
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 1 Pages: 66-80
Publisher: Basrah University جامعة البصرة

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Abstract

Tomato, is today the most popular garden vegetable in worldwide, because of its high consumption. Tomato contain a variety of phytochemical , such as lycopene , flavonoids, glycosides and other chemical constituents that have been beneficial protective effect. The present study carried out to evaluate the effect of two different doses (2 mg/kg BWand 4 mg/kg BW) of tomato extract against hydrogen peroxide-induced oxidative stress in albino rats. Thirty two adult male albino rats randomly divided into four equal groups were used in this study, the results revealed that administration of 0.75% H2O2 in drinking water (groupI) produced significant decline of antioxidant enzymes ( superoxide dismutase (SOD) , glutathione (GSH), catalase (CAT) ) and serum albumin concentration , with significant elevation of lipid peroxidation rate by estimation of malondialdehyde (MDA) and peroxynitrite radical (ONOO). Also, H2O2 caused significant increase in serum concentration of alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), and bilirubin . On the other hand the animals treated with H2O2 plus 4 mg/kg BW of tomato extract (groupIII) showed significant increase of SOD, GSH, CATand albumin with significant reduction of MDA ,ONOO, ALT, AST, ALP, and bilirubin comparing with control group. Depending on the above oxidant and antioxidant markers, it seems that 4 mg/kg BW. of tomato extract exert beneficial action protect against H2O2 induced oxidative stress in rats.

Keywords

Oxidative stress --- GSH --- Tomato extract --- ALT


Article
Antioxidant and Anti-Inflammatory Effect of Fruit Juice of Annona Muricata L (Soursop) During Ischemia Reperfusion Injury in Rats

Authors: Mustafa Taha Mohammed --- Sarah Isam Abbas
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 1 Pages: 118-123
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:OBJECTIVE:The study included knowledge The chemical components of the Soursop juice and determine whether Soursop prevents ischemia-reperfusion induced brain injuries in rats.METHODS:The chemical components of the prepared fruit extract were detectedits components of glycosides, proteins....etc and we were Determination of trace element and the estimation of the biochemical parameters: Blood samples were harvested from the rats,. Serum IL-10,Adiponectin and oxidative stress levels of Malondialdehyde ( MDA) and Glutathione ( GSH )were estimated.RESULTS: The results showed that the extract contain : glycosides, proteins, saponins, tannins, various phenolic compounds alkaloids , flavonoids, steroids and vitamine C . The results also showed that there were high concentrations of B, Fe , Zn respectively and low concentrations of Mn, Cu, pb,Cd respectively ,very low concentrations Ni ,Co , Se . The levels of oxidative enzymes and Inflammatory cytokines in group 3 were significantly lower than those in the group 2.Our results suggest that soursop treatment protects the rat brain against ischemia-reperfusion induced brain injuries.CONCLUSION: The present study confirm that the fruit extracts of Soursop (Annona Muricata L.) posses in vivo anti-inflammatory and antioxidant activity because of its content (glycosides , tannins , saponins ,proteins ,various phenolic compounds ,alkaloids , flavonoids, steroids and vitamine C )


Article
The Protective Effect of Radish (Raphanus sativus) Seeds Against the Oxidative Stress Induced by Sodium Nitrite in Male Rabbits (Oryctolagus cuniculus)
التاثير الواقي لبذور الفجل (Raphanus sativus) ضد الاجهاد التاكسدي المستحدث بواسطة نتريت الصوديوم في ذكور الارانب (Oryctolagus cuniculus)

Authors: Mukhtar K. Haba مختار خميس حبه --- Alia H. Ali عالية حسين علي --- Lena A. Abed-Alazeez لينا احمد عبد العزيز
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2016 Volume: 13 Issue: 1 Pages: 44-52
Publisher: Baghdad University جامعة بغداد

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Abstract

The aim of this study is to investigate the protective effect of Radish (Raphanus sativus) seed alcoholic extract 70% against oxidative stress induced by sodium nitrite NaNO2 Twenty five adult male rabbits were devided into five groups of (five rabbits in each group) and treated daily for 30 days. Group T1: intubated orally 20 mg/kg NaNO2, Group T2: intubated orally 20 mg/kg NaNO2 + 50 mg/kg of alcoholic extract from Raphanus sativus seeds, Group T3: intubated orally 20 mg/kg NaNO2 + 100 mg/kg of alcoholic extract from Raphanus sativus seeds, Group T4: intubated orally 20 mg/kg NaNO2 + 200 mg/kg of alcoholic extract from Raphanus sativus seed as well as Group C: control intubated orally distilled water. In comparison with normal rabbits there were significantly increase in the level of Methemoglobin (MetHb%) , lipid peroxidation indicator malondialdehyde (MDA), and significantly decrease in the level of glutathione (GSH) in the rabbits intubated NaNO2. In rabbits intubated NaNO2 + 50,100,200 mg/kg of alcoholic extract it was found there were significant decreased in the level of MetHb%, MDA, and increase in the level of GSH. In conclusion according to results obtained from this study approved that alcoholic extract of Raphanus sativus seeds in dose (50,100,200 mg/kg) have protective effect against oxidative stress induced by sodium nitrite in male rabbits.

أن الهدف الرئيسي لهذه الدراسة هي معرفة التاثير الواقي لبذور نبات الفجل على الاجهاد التاكسدي المستحدث بواسطة نتريت الصوديوم. استعمل في الدراسة الحالية 25 ارنب ذكر , قسمت الى خمس مجاميع كل مجموعة مؤلفة من 5 حيوانات. المجموعة الاولى (مجموعة السيطرة C) جرعت فمويا بجرعة من الماء المقطر مساوية لحجم الجرعة المعطاة لمجاميع المعالجة. المجموعة الثانية T1 جرعت فمويا ب 20 ملغم/كغم من مادة نتريت الصوديوم ولمدة 4 اسابيع . المجموعة الثالثة T2 جرعت فمويا ب 20 ملغم/كغم من مادة نتريت الصوديوم واعطيت المستخلص الكحولي لبذور نبات الفجل وبجرعة 50 ملغم/كغم. المجموعة الرابعة T3 جرعت فمويا ب 20 ملغم/كغم من مادة نتريت الصوديوم واعطيت المستخلص الكحولي لبذور نبات الفجل وبجرعة 100 ملغم/كغم. المجموعة الخامسة T4 جرعت فمويا ب 20 ملغم/كغم من مادة نتريت الصوديوم واعطيت المستخلص الكحولي لبذور نبات الفجل وبجرعة 200 ملغم/كغم. اوضحت نتائج هذه الدراسة حصول ارتفاع معنوي في نسبة الميتهيموكلوبين وناتج اكسدة الدهون وانخفاض معنوي لمحتوى الكلوتاثيون في الحيوانات المعاملة بمادة نتريت الصوديوم فقط, وكذلك حصول انخفاض معنوي في نسبة الميتهيموكلوبين وناتج اكسدة الدهون وحصول ارتفاع في محتوى الكلوتاثيون في الحيوانات المعاملة بمادة نتريت الصوديوم والمستخلص الكحولي200,100,50 ملغم/كغم . اعتمادا على نتائج هذه الدراسة نستنتج ان المستخلص الكحولي لبذور نبات الفجل50 و100 و 200 ملغم/كغم يمتلك الفعالية كمضاد للأجهاد التأكسدي المستحدث بمادة نتريت الصوديوم.

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