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Article
Immunohistochemical study for localization detection of insulin like growth factors(IGF1) in testiscular tissue of immature and mature male Wistar rats
دراسة كيمياء نسيجية-مناعية لكشف مواقع عامل النمو شبيه الأنسولين من النوع الأول IGF1 في أنسجة خصى ذكور الجرذان البالغة وغير البالغة

Author: jJabbar A. A. Al-Sa'aidi جبار عباس احمد الساعدي
Journal: journal of al-qadisiyah for pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2016 Volume: 1 Issue: 21 Pages: 24-31
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

The present study has been carried to investigate the immunological localization of Insulin-like Growth Factor 1 (IGF1) in pre- and post-pubertal stages and its involvement in male reproductive physiology of rats, Forty immature and mature male Wistar albino rats were used in the present study which assigned into 5 equal groups, 8 per each at 25th, 35th, 45th, 55th, and 65th day of age, male rats of each group have been anaesthetized. Testes tissue samples were obtained for immunohistochemical study. The results of immunohistochemical study showed the presence of IGF1 immunoreactivity in,sertoli cells, leydig cells and all cells that Constituent of seminiferous tubule, and showed density immunoreactivity increase during pubertal stage. Also observed the presence of IGF-1 immunoreactivity in mature sperm in seminiferous tubule lumen of post-pubertal testis.

أجريت الدراسة بهدف التحري عن التموقع المناعي لـعامل النمو شبيه الأنسولين النوع الأول في أنسجة الخصى خلال مرحلتي ما قبل البلوغ وبعده في ذكور الجرذان ومساهمتها في الوظيفة التكاثرية لذكور الجرذان، تم أستخدام 40 من ذكور الجرذان من سلالة الوستر البيضاء غير الناضجة والناضجة تم تقسيمها الى خمس مجموعات متساوية العدد إذ تضمنت كل مجموعة ثمان جرذان ذكوراً بأعمار(25 و35 و45 و55 و65) يوماً، تمت التضحية بثمان ذكور من كل عمر. أُخذت نماذج الخصى لغرض أجراء الدراسة الكيميائية النسجية المناعية. بينت نتائج الدراسة الكيميائية النسيجية المناعية وجود التفاعل المناعي لعامل النمو شبيهة الأنسولين IGF1في خلايا ليدك وخلايا سرتولي وجميع الخلايا الجنسية المكونة للنبيب المنوي ، كما اظهرت النتائج ان قوة و كثافة التفاعل المناعي لعامل النمو IGF1 يزداد عند البلوغ كما لوحظ وجوده في النطف الناضجة في تجويف النبيب المنوي عند مرحلة بعد البلوغ .


Article
Role of mRNA Binding Protein (HuR) Expression Level in Cancer Cells

Authors: Ibrahim Abdulmajeed Altamemi --- Rateb Fanokh Abo Khadher
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 1 Pages: 17-23
Publisher: Babylon University جامعة بابل

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Abstract

HuR is a mRNA-binding protein. Intracellular localization of HuR is mainly found within nucleus, but it could be translocate between the nucleus and cytoplasm. In the cytoplasm HuR canincrease half-life of certain mRNA target. Since cytoplasmic localization of HuR is essential for its activity, thus, HuR translocation in malignant cells could have prognostic indication. In the present study we aimed to evaluate the significance importance of HuR in the aggressiveness ofcolorectal adenocarcinoma. To achieve this goal, we have investigated itsexpression level in adenocarcinoma sample from Iraqi patients, 7through linking its expression with tumor histopathological variables (stage, grade, grade, and lymph node involvement), by using Immunohistochemical staining method. Study done on 40 colorectal cancer samples and their respective resection margins. Present study demonstrated that, the positive expression rate of integrin HuR in non-tumor colorectal mucosa was significantly lower than that of the colorectal cancer (CRC) tissue (P<0.005). Moreover, when CRC samples breakdown according to histopathological variables, significant differences in expression level of HuR protein when compared with different tumor stage, grade, and LN involvement depending on mean expression ±SE value (P< 0.05, P< 0.05, and p<0.05 respectively). Our results show that high cytoplasmic HuR expression is associated with a poor histologic differentiation, large tumor size, and poor prognosis in colorectal adenocarcinoma.


Article
The Study of HER-2/neu, ER/PR Expression Using Immunohistochemistry (IHC) in the Iraqi Breast Cancer

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Abstract

Background: Breast cancer is the most common cancer in woman that originates from the uncontrolled growth of abnormal breast cells. According to the Iraq Cancer Registry 2009, breast cancer ranks as the first between the ten common cancers in Iraq and there is a wide tendency to increase the rate of breast cancer in earlier age group. Estimation of estrogen (ER) and progesterone (PR) receptors status as well as human epidermal growth factor receptor 2(Her-2/neu) serve as specific guidance to select the patients whose benefit from endocrine therapy and provide prognostic information. The study aimed to assess the expression of ER/PR hormone receptor and Her-2/neu in breast cancer patients and correlation with various Clinicopathological aspects as a predictive biomarker.Method: The current study was performed from the period between Jan 2015-Feb 2015 in Department of Oncology that referred to Baghdad Teaching Hospital in the Medical City. Routine staining hematoxylin and eosin (H&E) for histopathological examination and immunohistochemistry (IHC) test for ER, PR and Her-2/neu expression was conducted in all cases.Results: This study includes total 30 cases of breast cancer. The age of patients were between 29-65 years with 47± 9.8 (mean ±SD). Majority of tumors are invasive ductal carcinomas represented 23(76.7%).There are significant differences between different scores were observed in IHC test for Her-2/neu status and showed 8(26.7%) out of 30 cases were positive expression.The ER and PR expression status was strongly associated and demonstrated in 21(70%) of cases.The most commune subgroup was Her-2/neu+, ER/PR+ seen in 10(33.3%) out of 30 breast cancer patients. Conclusion: HER-2/neu is positively expressed in about 26.7% of breast cancer cases.The study of ER, PR status showed higher rates of positive expression70% and was strongly associated. Using IHC examination the Her-2/neu+, ER/PR+ subgroup indicates the most common subtype 10 (33.3%) compared to other tumor subtypes in breast cancer.

Keywords

Breast cancer --- ER/PR --- Her-2/neu --- IHC


Article
Determination of Some Biochemical Markers for Breast Cancer Women in Baghdad
تقدير بعض العلامات البايوكيميائية للنساء المصابات بسرطان الثدي في بغداد

Authors: Hiba Abid Al-Hussein Hassan هبا عبد الحسين حسن --- Amani Mahmood Tauma أماني محمود طعمة --- Shatha Hamed Ataimish شذى حامد جويد
Journal: Al-Ma'mon College Journal مجلة كلية المامون ISSN: 19924453 Year: 2016 Issue: 28 Pages: 317-327
Publisher: AlMamon University College كلية المامون الجامعة

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Abstract

The most important tumor that develops from breast tissue is breast cancer: signs may have , mass in the breast; variation in shape; skin dimpling; bloody discharge from the nipple. The present study was conducted to have a clearer integrative idea on the impact of expression of estrogen and progesterone receptor. In addition CA15-3, CA-125 in sera of women with breast cancer in baghdad is compared with a healthy normal population. The paper studies two groups including 100 breast cancer women by using immunohistochemical analysis for evaluation of the Estrogen receptor (ER) and progesterone receptor (PR). Moreover, the sera of samples have been quantitatively measured for CA15-3, CA-125 by using ELISA method.This study statistically showed increased level of CA-125 among the patients group (54.07± 7.19U/ml) in comparison with healthy control group (8.57±4.93U/ml) with a highly significant increase between them (p< 0.0001). While CA15-3 in sera of patients groups revealed that there was a significant increased between its level of patients (17.28± 8.38U/ml) in comparison with healthy control groups (11.45±8.36U/ml) (P=0.001). Analysis of data showed a trend toward increasing ER and PR expression with age. Especially in menopausal age between 47-57 years compared to other age groups. The conclusion of this study indicated that ER &PR is overexpressed in breast cancer women especially in age between (47-57) years and elevated level of CA125 and CA15-3 in breast cancer women.

وضحت الدراسة تأثير ابراز او عرض مستقبل الاستروجين ومستقبل البروجسترون لدى النساء المصابات بسرطان الثدي في بغداد مقارنة بالأصحاء.حددت مجموعتي الدراسة والتي تتضمن( 100) عينه لنساء مصابات بسرطان الثدي تم استخدام اختبار المناعي النسيجي الكيمياوي IHC في تقييم مستوى مستقبل الاستوجين والبروجسترون في الخلايا النسيجية المسرطنة. فضلا عن ذلك فقد اختبر مصول المرضى للتحري عن مستوى الضد الجيني السرطاني (125 ) بطريقة مقياس المر المناعي للانزيم المرتبط ELISA .كشف التحليل الاحصائي ارتفاع في مستوى الضد الجيني السرطاني( 125 ) في مصول النساء المصابات بسرطان الثدي(54.07± 7.19) مقارنة بمصول الاصحاء(8.57±4.93) وبفارق احصائي عالي المعنوية ((p<0.0001 بينما بين مستوى الضد الجيني السرطاني (15-3 ) ارتفاعاً طفيفاً في مصول المصابات بسرطان الثدي (17.12± 8.38) مقارنةً بالنساء غير المصابات(11.45±8.36) وبفرق احصائي معنوي (P=0.001) وكذلك بين التحليل احصائيا عن ارتباط مهم بين زيادة عرض او ابراز مستقبل الاستروجين وبروجسترون وعمر النساء المصابات بسرطان الثدي بدون فارق احصائي يذكر. خصوصا في عمر انقطاع الطمث (47-57) سنة مقارنةً بالأعمار الاخرى .بينت هذه الدراسة اهمية ابراز مستقبل البروجسترون ومستقبل الاستروجين لدى النساء المصابات بسرطان الثدي وخصوصا في الاعمار (47-57) وارتباطه المهم بارتفاع مستوى الضد الجيني السرطاني (125 ) والضد الجيني السرطاني( 15-3 ) لدى النساء المصابات بسرطان الثدي.

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