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and Medical Immunohistochemical expression of Aromatase in malignant breast tumours
التعبيرالنسجي-الكيميائي-المناعي للارومتيز في أورام الثدي الخبيثة

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Abstract

Twenty tumour biopsies of Iraqi breast cancer female patients were examined for immunohistochemical (IHC) expression of aromatase. The expression was correlated with age and histopathological type, grade and clinical staging of tumour. Results revealed that out of the 20 investigated cases, 75.0% showed positive IHC expression of aromatase, which was dis- tributed as 35.0% for score 2+ and 40.0% for score 3+. The remaining ve negative cases (25.0%) were observed to have the lowest age mean (38.6 ± 4.4 years), which was signi cantly different from the recorded age mean in patients with the score 2+ (53.6 ± 3.2 years; p = 0.008) or 3+ (49.4 ± 2.8; p = 0.04), but no signi cant difference was observed between age means of positive cases. A histpathological examination of tumours revealed that they were invasive ductal carcinoma (IDC) but of two types: tubular IDC (5 cases; 25.0%) and 15 cases (75.0%) were de ned as not otherwise speci ed (IDC-NOS). In tubu- lar IDC, most of cases (80.0%) had 3+ score, while in IDC-NOS, it was 26.7%. Such difference was signi cant (p = 0.023). Distributing patients by grade and stage of tumours revealed no signi cant difference in IHC scoring of aromatase or age. It is possible to conclude that positive IHC expression of aromatase is tended to occur in older ages (around 50 years) of breast cancer patients.

اجريت الدراسة على عشرين مريضة عراقية مصابة بسرطان الثدي. اخذت مقاطع نسجية للورم واجري التقييم النسجي-الكيميائي-المناعي ل رومتيز. ارتبط التعبير ل رومتيز مع التقدم في العمر ونوع النسيج والرتبة والتدريج السريري للورم. واظهرت النتائج ان ٪75.0 من المريضات كانت موجبة لفحص ا رومتيز، والتي كانت موزعة على النحو( ٪35.0 للـ +score2 و٪40.0 للـ +3 score(، وقد لوحظت خمس حا ت سالبة لفحص ا رومتيز )٪25.0( وكان لديهم أقل معدل العمر )38.6 ± 4.4 سنة(، واختلف معنويا عن المريضات الموجبة للفحص 3.2 ± 53.6( +2 score سنوات؛ 0.008 = P( و± 49.4( +3 score 2.8؛ 0.04 = P(، ولكن لم ي حظ أي اخت ف معنوي بين عمر العينات الموجبة للفحص. وكشف الفحص النسجي المرضي للمريضات انه من نوع سرطان القنوات الغازي، ولكن بنوعين: ا نبوبي 5 tubular IDC حا ت )٪25.0( و15 حالة )٪75.0( تم تعريفها بأنها )IDC-NOS(. في النوع ا نبوبي وأن معظم الحا ت )٪80.0( موجبة +3 score، بينما في IDC-NOS، كان ٪26.7. وهنا الفرق معنويا )0.023 = p(. فمن الممكن أن نستنتج ان التعبير IHCا يجابي ل رومتيز ويميل للحدوث في ا عمار المتقدمة )حوالي 50 عاما( من مريضات سرطان الثدي.


Article
P57Kip2 immunostaining, a diagnostic marker in differentiating complete hydatidiform mole from its mimics
P57Kip2 المناعية ، علامة تشخيصية في التمييز بين الخلد المائي الكامل من مقلداته

Author: Ava T. Ismael
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2016 Volume: 20 Issue: 2 Pages: 1316-1322
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: The distinction of hydatidiform mole from hydropic abortion remains a problem because of interobserver and intraobserver variability. This study aimed to determine the utility of p57Kip2 as a diagnostic marker in differentiating complete hydatidiform mole from its mimics.Methods: A total of 97 formalin fixed paraffin embedded material including forty cases of complete hydatidiform mole, 36 cases of partial hydatidiform mole and 21 cases of hydropic abortion were selected randomly from the files of histopathology laboratory of Maternity Teaching Hospital in Erbil. The samples were reviewed by two pathologists, afterward; immunohistochemical staining was performed by using a p57Kip2 marker. We considered p57Kip2 positive only if nuclear p57Kip2 staining was identified in at least 10% or more of all in a tissue section. Results: Negative immunostaining was seen in 77.5% of the complete hydatidiform mole in both villous cytotrophoblast and stromal cells. In contrast, 86.1% of partial hydatidiform mole showed positive immunostaining for p57Kip2. All cases of hydropic abortion 100% were positive for p57Kip2 immunostaining. In all gestations, p57Kip2 was strongly expressed in decidua which served as internal positive control. The concordance between the initial histological diagnosis and p57Kip2 immunostaining was statistically significant (P <0.001). Conclusions: p57Kip2 immunostaining is a highly sensitive and specific marker for diagnosis and classification of hydatidiform mole. p57Kip2 staining has the advantage of differentiating hydropic abortuses from the complete hydatidiform mole.


Article
Immunohistochemical Localization of Epstein Barr Virus- Latent Membrane Protein 1 in Breast Cancer Tissues

Author: Saad Hasan Mohammed Ali *,Shakir H. Mohammed Al-Alwany**, Ghanim I.A. Al-Wadi *
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 2 Pages: 234-240
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACK GROUND:Epstein- Barr virus (EBV) is well known to infect the vast majority of the world's adult population and has recently been reported in many literatures for its association with a broad spectrum of benign and malignant tumors, including breast cancer. OBJECTIVE: To analyze the relations and impact of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) expression on a group of tissues with primary invasive breast cancers.PATIENTS AND METHODS: Forty- nine (49) formalin-fixed, paraffin- embedded breast tissues were obtained in this study; (34) biopsies from breast cancers (BC) and (15) from apparently normal breast autopsies control group. Detection of expression of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) was done by HRP/DAB immunohistochemistry detection kit (an immunoenzymatic antigen detection system for immunohistochemistry techniques) using specific primary antibodies for EBV-LMP 1.RESULTS: Detection of EBV-LMP 1 - immunohistochemical (IHC) reactions in tissues with BC was observed in 11 out of 34 (32.4%), while in healthy breast tissues in the control group was detected in 13.3% (2 out of 15). The difference between the percentages of EBV-LMP 1 detection in BC tissues & control group was statistically not significant (P >0.05).While no significant differences were observed between EBV-LMP 1 detection in BC tissues & the age of patients as well as grade of invasive breast cancers (P value > 0.05).CONCLUSION: Our results indicate that EBV could have a role in the pathogenesis of subset of breast cancers and contribution in their carcinogenesis. .

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