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Article
Antibiotics Resistance of Isolated Bacteria from Patients with Urinary Tract Infection
مقاومة البكتيريا المعزولة من المرضى المصابين بالتهاب المجاري البولية للمضادات الحيوية

Author: Abbas Atyia Hammoudi عباس عطية حمودي
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2017 Volume: 13 Issue: 4 - part 1 Pages: 233
Publisher: Diyala University جامعة ديالى

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Abstract

From the period of March to thirty August 2015, 210 urine samples were collected from patients with signs and symptoms of UTIs with an age range of 20-60 years, who referred to outpatient department of AlKarama teaching hospital, Baghdad. Out of 210 samples of urine 150(71.43%) were positive culture and 50(23.81%) were negative culture while 10 (4.76%) were showed contaminated urine culture. The common isolated bacteria were E. coli 45 (30%), K. pneumonia 33(22%)as Gram negative bacteria respectively, while Gram positive bacteria was Staphylococcus aureus 42(28%). The other bacteria were Enterobacter spp.20 (13.3%), Proteus spp. 6(4%), and Pseudomonas aeruginosa4 (2.7%). Susceptibility testing were used with the following antibiotic disks: Ciprofloxacin, Amikacin, Nitrofurantion, Trimethoprim-Sulfamethoxazol, Gentamicin, Pipracillin , Nalidixic acid , Tetracycline , Imipenem , Ceftriaxone , and Cefotaxime. The percentage of susceptibility showed variable susceptibility to the antibiotics used. All bacteria showed (100%) susceptible to Imipenem while all bacteria showed (100%) resistant to Tetracycline. Pseudomonas aeruginosa isolated bacteria was showed multidrug resistant (MDR); seven antibiotics: Nitrofurantion, Trimethoprim-Sulfamethoxazol, Pipracillin, Nalidixic acid, Tetracycline, Ceftriaxone and Cefotaxime.

جمعت 210 عينة ادرار للفترة من شهر نيسان ولغاية شهر اب 2015 من المرضى الذين يعانون من اعراض وعلامات لالتهاب المجاري البولية الذين يراجعون العيادة الخارجية في مستشفى الكرامة التعليمي. تم تشخيص 45 عزلة(30%) للاشيريشيا القولونية و33 عزلة للكليبسيلا الرئوية و42 (28%) عزلة للعنقوديات الذهبية و20 (13.3%)عزلة لجنس البكتيريا المعوية و 6 (4 %) عزلات لجنس البروتيس و 4 (2.7%) عزلات للزوائف الزنجارية.اظهرت العزلات حساسية متباينة للمضادات الحيوية المستخدمة .اظهرت جميع العزلات حساسية للمضاد اميبينيم بينما اظهرت جميع العزلات مقاومة للمضاد تتراسايكلين. اظهرت بكتيريا الزوائف الزنجارية مقاومة متعددة للمضادات الحيوية :سبعة مضادات حيوية: نايتروفيوريشن, تراي ميثوبريم-سلفاميثوكسازول,ببراسلين,نالديسك اسد,تتراسايكلين ,سيفترياكسون و سيفوتاكسيم .


Article
Sequencing characterization of housekeeping genes among Klebsiella pneumoniae isolated from burn patients

Authors: Abbas Atyia Hammoudi --- Azhar N. Hussein
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 111-119
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Burn wound infections are one of the most important impairments that occur in the acute period following injury and colonization by the pathogenic agents, including gram-positive, gram-negative bacteria and yeasts. The study included 210 clinical swab samples have been collected from burn- wound patients and cultured on blood agar, MacConkey agar and Eosin methylene blue agar, the period from 1/3/2016 to 30/8/2016 at different hospitals in Baghdad city. By microscopic characterizations, morphological and biochemical reactions, the results showed that 42 (37.5 %) isolates belong to Klebsiella pneumoniae. The analysis of (10) clinical origin of Klebsiella pneumoniae isolates by multilocus sequence typing show the relationship between the local and global isolates which belonged to 7 housekeeping genes (rpoB;beta-subunit of RNA polymerase, gapA; glyceraldehyde 3-phosphate dehydrogenase, mdh; malate dehydrogenase, pgi; phosphoglucose isomerase, phoE; phosphorine E, infB; translation initiation factor 2, tonB; periplasmic energy transducer). The present study the results showed the 10 isolates of K. pneumoniae were identified into different sequence type (ST): ST 14 and 15 for (IQK1, IQK2, IQK3, IQK4, IQK5, IQK6 and IQK7), ST 266, 54, 709, 728 and 1177 for (IQK8 and IQK9) and ST 665, 975 and 2149 for (IQK10). In addition, the result showed 100% identities with previously reported genes. There was no information on the sequence type (ST) (an allelic profile) of K. pneumoniae in Iraq. According to the results of the present study, the most occurrence clones found in Baghdad hospitals were endemic ST14 and 15, which accounted for 70% of the isolates (n=10). The presence of the ST14 and 15 clones in Iraq which came closer to global (14 and 15 STs) clones might be indicating intercontinental transmission because these clones were added to the list of the strains that isolated from different countries.

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