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Article
Association of Helicobacter Pylori Infection and Gastric Cancer

Author: Shurooq A. Lafta, Abdul Hussein M. ALFaisal
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 1 Pages: 1-9
Publisher: Baghdad University جامعة بغداد

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Abstract

Helicobacter pylori (H. pylori) is the most important etiologic factor for gastric cancer. It is one of the most common human pathogens, which colonizes in the mucus layer of the gastric epithelium in more than 50% of the population. The study include 78 samples of gastric cancer in addition to 42 blood samples. The results from 78 gastric cancer samples showed that gastric cancer can occur red at any age, but it increases in older ages and the incidence of H. pylori infected positive gastric cancer (HIP-GC) is higher than of H. pylori infected negative gastric cancer (HIN-GC). Male is the predominant in both groups and female affected by H. pylori more than male and the intestinal type was the predominant type.


Article
Prevalence of H pylori in obese attending Obesity therapy Unit

Author: Mumtaz K Hana*, Abdulhadi Alrubaie **, Mohanad abdulmahdi Ahmad ***, Faris Abdulkarim ****
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2017 Volume: 13 Issue: 2 Pages: 76-78
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Obesity is an increasing health problem in developed countries and has grown into a major global epidemic. Recent studies suggested colonization of the stomach by Hpylori might affect gastric expression of appetite- and satiety-related hormone and patients cured of H pylori infection gained weight. Obesity and Helicobacter pylori (H. pylori) are important because of the problems they lead and their frequency of occurrence.Objectives: To find out the prevalence of H. pylori infection in obese.Type of the study:A cross-sectional studyMethods: A total of 32 obese female admitted to the study. Body mass indices (BMI) of all subjects were calculated H. pylori positivity were studied.Results: The age range of the obese was (20-59) and the BMI (was 25-55 kg/m²). 26 obese (81.25%) were H pylori positive, and all obese above 45 were H pylori positive. Conclusions: Obesity and h pylori are closely associated and the prevalence of h pylori increased with obesity. We consider that H. pylori infection can be a risk factor for obesity and almost all obese patients above 45 years were sero positive. However, further studies evaluating more subjects are required. And we need to do national survey about h pylori prevalence in our community


Article
Study the relation of gastric cancer with helicobacter pylori infection among dyspeptic patients

Author: Wasan Hatem Rzooqi , Muna Turkey Al-Mossawei
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 3 Pages: 159-168
Publisher: Baghdad University جامعة بغداد

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Abstract

Helicobacter pylori (H. pylori) is the main causative agent of gastrointestinal diseases including chronic gastritis, peptic ulcer associated disorders, gastric and duodenal carcinomas leading to morbidity and mortality in humans. This research was conducted to study the relation of gastric cancer with H.pylori infections through some invasive methods(Histology, Direct biopsy smear, Biopsy urease test and culture methods). One hundred and twenty five dyspeptic patients were subjected to esophageal gastroduodenoscopy in Baghdad Teaching Hospital/ Endoscopy unit from10/1/2013 to 8/1/2014, gastric biopsy samples were applied for microbiological analysis. The results showed that there was a relationship between H. pylori infection occurrence and endoscopically diagnosed Gastric cancer (3.2%); it was recorded highest H. pylori isolation (45.45%) from gastric ulcer patients followed by (25%) from gastric cancer patients. Histological examination recorded that active gastritis is found to be the most infected case (93.75%) with H. pylori followed by gastric adenocarcinoma (66.66%) gastric lymphoma (50%), so the study recorded a significant association between H .pylori infection and gastric cancer.


Article
Association of Helicobacter Pylori Infection with Diabetes Mellitus in Baquba-Diyala Province

Author: Hasan, A.SH*, Zainab H. AL-Azawi**, Shiama,a R. AL-Salihy***
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2017 Volume: 16 Issue: 3 Pages: 285-289
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Infection with H. pylori has been recognized as a public health problem worldwide. Studies to address the association of H. pylori infection with diabetes mellitus (DM) had yielded controversial results.OBJECTIVE:To figure out the association between H. pylori infection and DMtype 1 and 2 in Baquba city, Iraq . PATIENTS AND METHODS:This cross-sectional case control study was conducted in Baquba-Diyala province for the period from August 2015- March 2016. 82diabetic patients (67 with type 2 and 15 with type 1) and 23 healthy non-diabetic individuals were enrolled. The DM patients include 45 (54.9%) females and 37(45.1%) males. The mean age ±SD of type 2 DM patients was51.37± 10.84 years,while the mean age ±SD of patient with type 1 DMwas 23.66 ± 6.99 years. The patients were collected from the outpatient clinic of Baquba General Teaching Hospital according to the WHO definition criteria of the DM. Blood samples were collected, sera were separated and tested for the presence of anti-H. pyloriIgG antibody using the commercially available Enzyme- linked immunosorbant assay kit.Statistical analyses were done using the Statistical Package for Social Science (SPSS) Version 18, and P value < 0.05 was considered significant.RESULTS:The results showed that the anti- H. pylori positivity rate among patients with diabetes mellitus and controls was 35.2% and 9.5% respectively, with statistically insignificant difference (p= 0.54). The anti- H. pyloripositivity rate was slightly higher in type 2 DM than in type 1 DM (29.5 % vs 5.7 %, p=0.9).Diabetic male had insignificantly higher positivity rate compared to female ((29.5% vs26.8, p= 0.096%). Furthermore, higher positivity rate was found in the age group 30-50 years.CONCLUSION:The anti-H. pyloripositivity rate is insignificantly higher among diabetes mellitus patients; particularly type 2 diabetes mellitus than non-diabetic individuals in Baquba cit, Iraq .


Article
The impact of Helicobacter pylori infection on iron deficiency anemia in pregnancy

Authors: Miami Abdul Hassan Ali --- Eaman Marouf Muhammad --- Ban Hadi Hameed --- Hala Hashim Hasoobe
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2017 Volume: 6 Issue: 2 Pages: 60-64
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

BACKGROUND: Anemia in pregnancy has different adverse effects on pregnancy outcome, andiron deficiency anemia (IDA) is the most common cause of anemia during pregnancy. Recent studieshave suggested an association between Helicobacter pylori and IDA during pregnancy.OBJECTIVE: The aim of this study was to evaluate the impact of H. pylori infection on IDA in pregnancy.PATIENTS AND METHODS: This was a case–control study carried out at Al‑Yarmouk TeachingHospital between January 1 and August 1, 2016. The study included 100 pregnant women dividedinto two groups: control group with normal hemoglobin (Hb) and a study group with IDA. Both groupswere subjected to H. Pylori test.RESULTS: The mean Hb level of the patients was 9.4 ± 0.8 g/dl. Fifty cases had an Hblevel <11 g/dl and fifty cases with Hb ≥11 gm/dl. Thirty‑two percentage of the study group wereseropositive for anti‑H. pylori IgA compared to 4% of the control group, and this difference wasstatistically significant at P < 0.001.CONCLUSION: There was a positive correlation between IDA during pregnancy and H. pylori‑positivecases.


Article
Prospective study on effect of Helicobacter pylori on gastroesophageal reflux disease
دراسة مستقبلية عن تأثير هيليكوباكتر بايلوري على مرض الجزر المعدي المريئي

Author: Sabah Jalal Shareef
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2017 Volume: 21 Issue: 2 Pages: 1696-1700
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: The Helicobacter pylori infections role in etiology of peptic ulcer is well known, but its role in gastroesophageal reflux disease is one of the important issues which has to be confirmed. We tried to find out the effect of Helicobacter pylori infection on gastroesophageal reflux disease.Methods: The current study was done on 100 patients with gastroesophageal reflux disease from January 1st to June 30th, 2014 in Rizgary Teaching Hospital, Erbil city. The diagnosis was made by history, clinical examination, and endoscopy. Helicobacter pylori infection was confirmed by gastric biopsy and histopathological examination. We tried to find out the effects of Helicobacter pylori infection in gastroesophageal reflux disease patients and its eradication on their symptoms. The data was analyzed with the statistical package for the social sciences (version 18).Results: The mean age ± SD of participants was 37.13 ± 12.5 (17-75 years). The prevalence of Helicobacter pylori infection was 75%. The endoscopy showed that 50 out of 75 patients had erosive esophagitis and 25 out of 75 patients had normal appearance known as non-erosive esophagitis. The study showed no significance of its eradication on symptoms of gastroesophageal reflux disease.Conclusion: The effect of Helicobacter pylori infection in gastroesophageal reflux disease patients was significant regarding endoscopic finding while inversely related to symptoms severity. The eradication of infection did not cause improvement in symptom severity i.e. triple therapy not advised in the course of treatment.


Article
Study the Relationship between Helicobacter pylori Infection and ABO Blood Groups and Rh Status

Author: Hasan Abd Ali Khudhair
Journal: Journal of Education for Pure Science مجلة التربية للعلوم الصرفة ISSN: 20736592 Year: 2017 Volume: 7 Issue: 3 Pages: 48-56
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

AbstractThis paper was aimed to investigate the association between the ABO blood groups and H.pylori infection and to determine how much endoscopic findings are predict for the presence ofactive H. pylori infection. One hundred and twenty dyspeptic patients (as diagnosed by endoscopyand confirmed by histopathology) and sixty apparently healthy individuals were enrolled in thisstudy. All subjects were underwent ABO blood grouping and Rhesus (Rh) systems determination.The results reveals higher frequency present of blood type O and the lowest frequency present ofblood types A and AB among H. pylori patients compared to positive control group. The endoscopicfindings showed low sensitivity in the diagnosis of H. pylori associated gastritis. In conclusion, thereare considerable positive relationship between H. pylori infection and blood type O.


Article
Comparison Study Among Some Methods That Used For Detection of Helicobacter pylori In Patient's With Gastrointestinal Disease
دراسة مقارنة بين بعض الطرق المستخدمة للتحري عن بكتريا المعدة الحلزونية لدى المرضى المصابين بأمراض المعدة والامعاء

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Abstract

The study aimed to comparison among some methods that used for detection Helicobacter pylori in patient's with gastroduodenal disease and evaluate the beast method. The study samples collected from endoscopy unit in Baqubah Teaching Hospital for the period (29-9-2015 to 25-2-2016). They study included 30 case patients with gasteroduodenal disease also it included a study on (10) samples of apparently healthy individuals (control) as comparison group. The results showed the detection of Helicobacter pylori directly from biopsy by Polymerase Chain Reaction (PCR) recorded heights percent among other tests which are included: rapid urease test for biopsy, Rapid anti H. Pylori test (IgG) and bacterial culture. The percentage of detection H. Pylori in patients group by these four tests were 80%, 56.67%, 43.33%and 10% respectively. There were significant differences (P ≤ 0.001) among different tests. The present study concluded that the PCR technique is suitable for accurately detection of these bacteria directly from gastric biopsy specimens and the difficulty of culturing H. pylori from samples taken from patient's with gastroduodenal disease which handlers treatments of antibiotics and proton pump inhibitors (PPI)

هدفت الدراسة الى المقارنة بين بعض الطرق المستخدمة للتحري عن بكتريا المعدة الحلزونية Helicobacter pylori لدى المرضى المصابين بأمراض المعدة والامعاء وتقييم الطريقة الافضل، شملت الدراسة على (30) حالة مرضية لأشخاص مصابين بأمراض المعدة والامعاء. وكذلك شملت الدراسة (10) عينات من اشخاص غير مصابين بهذه الامراض )السيطرة). حيث امكن الحصول على هذه العينات (خزعات نسيجية و دم) من وحدة الناظور في مستشفى بعقوبة التعليمي للفترة (29 - 9 - 2015 الى 25 - 2 - 2016)، اظهرت النتائج ان الكشف الجزيئي بتقنية الـ PCR عن بكتريا H. pylori مباشرة في الخزعات النسيجية للمرضى جاء بأعلى نسبة بين الاختبارات الاخرى ويليه اختبار اليوريز (انظيم اليوريا) السريع للخزعة النسيجية، ومن ثم اختبار للتحري عن الأجسام المضادة (IgG) لبكتريا H. pylori في المصل (Serum) واقل نسبة للتحري عن هذه البكتريا كانت بواسطة اختبار الزرع البكتيري. كانت نسبة الكشف عن بكتريا H. pylori في مجموعة المرضى بواسطة الاختبارات السابقة هي 80%، 56.67%، 43.33%، 10% على التوالي، اظهرت نتائج التحليل الاحصائي على وجود علاقة معنوية P ≤ 0.001 بين الاختبارات المستخدمة. بالاعتماد على نتائج هذه الدراسة نستنتج افضلية طريقة الكشف الجزيئي بتقنية الـ PCR عن بكتريا H. pylori اذ يكون ذو كفاءة عالية الخصوصية والحساسة للكشف عن هذه البكتريا مباشرة من عينات الخزعات النسيجية المعدية. وصعوبة استنبات بكتريا H. pylori من العينات المأخوذة من المصابين بأمراض المعدة والامعاء المتناولين للعلاجات المضادات الحيوية ومثبطات مضخة البروتونات PPI.


Article
Detection of, cagA and vacA Helicobacter pylori Virulence Genes in Gastric Biopsies of Patients with Gastroduodenal Disease using Polymerase Chain Reaction (PCR) Technique
التحري عن جينات ضراوة البكتريا الحلزونية ureA, cagA and vacA في خزعات نسيجة من معدة مصابين بأمراض المعدة والاثني عشر باستخدام تقنية تفاعل الكوثرة (PCR)

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Abstract

The objective of current study was to detect the prevalence of Helicobacter pylori by identifying 16SrDNA and to determine the virulence genes (ureA, cagA and vacA) in biopsy specimens from patients suffering gastroduodenal disease using polymerase chain reaction (PCR). Forty samples were obtained by gastroenterologists during endoscopy from gastric antral of suspected individual attending endoscopy unit at Baqubah Teaching Hospital, Diyala, Iraq, during the period from September 2015 to February 2016. According to the endoscopic finding the patients were allocated into four groups of gastroduodenal diseases and control, which include gastritis (GS), duodenal ulcer (DU), gastric ulcer (GU), gastric cancer (GC), their rates were 30% (12), 20% (8), 17.5% (7), 7.5% (3) and 25% (10), respectively. DNA was extracted from the biopsies and subsequently used for PCR detection of H. pylori and the virulence genes using specific primers. The results shows that 60% of samples were positive for H. pylori, of these positive samples, 91,66%, 66.66%, and 48.83%, were shown to have the virulence genes, ureA, cagA, and vacA, respectively. It is important to mention that cagA shown the highest prevalence rate in gastric cancer cases in comparison with vacA gene. further studies are required to study the link between cagA gene and gastroduodenal diseases. In conclusion the result of present study provide important information concerning the prevalence of virulence genes of H. pylori.

هدفت الدراسة الاستدلال على وجود البكتيريا الحلزونية Helicobacter pylori بالكشف عن المورث مشفر الرنا الرايبوسومي 16أس (16SrDNA) وتحديد امتلاك هذه البكتريا لمورثات الفوعة: المورث المشفر للوحدة A من انزيم اليوريز (ureA gene) و المورث المقترن بالسمية الخلوية cagA ومورث تشفير سم التحوصل الخلوي vacA ، في عينات خزعة النسيج من مصابين بأمراض المعدة والاثني عشر باستخدام تفاعل سلسلة أنزيم الكوثرة (PCR). جمعت أربعين خزعة نسيجية بأشراف طبيب الجهاز الهضمي خلال التنظير من غار المعدة من مرضى محالين لوحدة الناظور في مستشفى بعقوبة التعليمي/ ديالى-العراق، خلال الفترة من سبتمبر 2015 إلى فبراير 2016. وفقا لفحص المنظار توزع المرضى الى اربع مجاميع مرضية ومجموعة ضابطة، ضمت التهاب المعدة (GS) وقرحة الاثني عشر (DU)، وقرحة المعدة (GU)، وسرطان المعدة (GC) ، شكلت نسبها 30٪(12)، 20٪ (8) و 17.5٪(7)، 7.5٪(3) و 25٪ (10)على التوالي. استخلص الحمض النووي الريبوزي منقوص الاوكسجين من خزعات النسيج وكشف عن البكتيريا وعن مورثات الفوعة بتقنية تفاعل سلسلة أنزيم الكوثرة PCR باستخدام بادئات محددة. بين الكشف ان 60٪ من خزعات النسيج في الأمراض المذكورة أعلاه موجبة للبكتريا الحلزونية، وكانت معدلات تواجد مورثات الفوعة في العينات الموجبة 91.66٪ و 66.66٪ و 48.83٪ للمورثات ureA gene و cagA gene و vacA gene على التوالي. ومن الأهمية بمكان الإشارة الى ان المورث cagA gene أعطى أعلى نسبة في حالات سرطان المعدة مقارنة بالمورث vacA gene، وخلص إلى احتمال ارتباط المورث بالمرض. ان هذه الدراسة توفر معلومات هامة بشأن انتشار مورثات فوعة البكتريا الحلزونية.


Article
The inhibitory effects of some natural substances on H. Pylori
التاثير التثبيطي لبعض المواد الطبيعية على جرثومة الملوية البوابية Helicobacter pylori

Author: Eman Yousif Thanoon AL-Noaimi1, Amera Mahmood Al-Rawi2 ايمان يوسف ذنون1
Journal: kirkuk university journal for scientific studies مجلة جامعة كركوك - الدراسات العلمية ISSN: 19920849 / 26166801 Year: 2017 Volume: 12 Issue: 3 Pages: 145-156
Publisher: Kirkuk University جامعة كركوك

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Abstract

This study aimed to use natural alternatives to detect its inhibitory effect on H. pylori due to its low toxicity and flexibility in use as well as its efficiency in treatment.detection an the inhibitory activity of ( ginger , apple vinegar , mastic (gum,oil) , pomegranate peel and curcumin ) was done and Minimum Inhibition Concentration (MIC) was estimated. the results showed that the MIC of pomegranate peel ( 0.048828125 ) mg / cm3 so it is more efficient than curcumin (0.09765625 ) mg / cm3 and ginger ( 1.5625 ) mg / cm3 while there is no effect of mastic gum , mastic oil and apple vinegar on H. pylori.We conclude that these natural substances may be used as treatment assistant products to reduce H. pylori resistance to antibiotics

هدفت الدراسة الى استخدام بدائل طبيعية لبيان تاثيرها التثبيطي على جرثومة الملوية البوابية Helicobacter pylori لقلة سمية موادها ومرونتها العالية في الاستخدام وكفاءتها في المعالجة حيث تم التحري على الفعالية التثبيطية للمواد الطبيعية التالية ( قشر الرمان، كركم، زنجبيل، المستكي، زيت المستكي وخل التفاح ) وقياس التركيز المثبط الادنى للمواد المثبطة للجرثومة. بينت النتائج ان (MIC) Minimum Inhibition Concentration لقشر الرمان (0.048828125 ملغم / سم3) ويعد الاكثر تاثيرا من الكركم (0.09765625 ملغم / سم3) والزنجبيل (1.5625 ملغم / سم3) في حين لم يبدي كل من المستكي وزيت المستكي وخل التفاح تاثيرا على الجرثومة ,لذا نستنتج انه يمكن استخدام المواد الطبيعة المؤثرة كمنتجات ساندة للعلاج من اجل تقليل مقاومة الجرثومة للمضادات الحيوية.

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