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Article
Association between MethylenetetrahydrofolateReductase (MTHFR) GenePolymorphisms and breast cancer in sample of Iraqi women
العلاقه بين تعدد الاشكال للجين مثيل تتراهايدرو فوليت المختزل(C677T) وسرطان الثدي في عينة من النساء العراقيات

Authors: Ehab D. Salman أيهاب داوود سلمان --- Ezeddin A. Al Bayyar عزالدين عطيه البيار --- Reem K. Ibrahim ريم خالد ابراهيم
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2017 Volume: 58 Issue: 1C Pages: 447-453
Publisher: Baghdad University جامعة بغداد

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Abstract

Folate metabolism is fundamental and essential for DNA structure synthesis and repair. Change in genes that participate in folate metabolism can be linked with different types of malignant tumor, Therefore, this study was conducted to find out the association between methylenetetrahydrofolatereductaseMTHFR gene polymorphisms and risk of breast cancer in a sample of Iraqi patients. One Single Nucleotide Polymorphism ( SNP) including MTHFR C677T was calculated using a tetra primer ARMS PCR experiment assay. The results explained that (MTHFR C677T) consists of three genotype (CC, CT, TT), The CC genotype was the most frequent in patients and control group ( 40.00%) and(60.00%) ,respectively, while the lowest frequency was for TT genotype(26.25%) in patients group .This study also revealed that the higher frequency 56.88 % for allele C and 43.12 % for T allele in patients group . On the other hand the Odd Ratio ( O.R.) for CC, CT , TT genotypes were 1.073, 0.862 and 1.148 respectively, and the genotypes TT show a highest O.R.

ايض الفوليك ضروري لبناء الحمض النووي واصلاحه . أن التغايرات في الجينات التي تشارك في التمثيل الغذائي لحامض الفوليك يمكن أن تترافق مع عدة أنواع من الأورام الخبيثة، لذلك أجريت هذه الدراسة لمعرفة العلاقه بين تعدد أشكال النوكليوتيدات المــــفردة لجـــــــــــــين مثيل تتراهايدرو فوليت المختزلMethylenetetrahydrofolateReductase (MTHFR) وخطر الإصابة بسرطان الثدي في عينة من المرضى العراقيين .أختير نوع واحد من تعدد أشكال النوكليوتيدات المفردة Single Nucleotide Polymorphisms (SNPs) وهو (C677T) للجين (MTHFR) ، واستعملت طريقة tetra primer ARMS PCR assay لتحديد هذا النوع من تعدد أشكال النوكليوتيدات المفردة للجين.اشارت النتائج الى ان (MTHFR) يتكون من ثلاثة انماط جينيه (CC,CT, TT), وان النمط الجينيCCكان اعلى تكرار لدى المرضى ومجموعة السيطرة بنسبة (40.00%) و (60.00%) على التوالي . بينما كان النمط الجيني TT اقل تكرارا في مجموعة المرضى حيث بلغت نسبته(26.25%)، ايضا اشارت هذه الدراسة الى أن اعلى تكرار كان للأليلC وبلغت نسبته56.88 % مقارنة 43.12 % للأليلT في مجموعة المرضى وقد بلغت نسبة الأرجحية للأنماط الجينية(CC,CT ,TT) 1.148 , 0.862 و 1.073 على التوالي , وأظهر النمط الجينيTT أعلى نسبة أرجحية .


Article
6.CHOLELITHIASIS FOLLOWING BARIATRIC SURGERY: A NEW APPROACH TO DEAL WITH

Authors: Sajid H.A. Al-Helfy ساجد حميد الحلفي --- Qasim S. Almayah قاسم شرهان المياحي --- Risala H. Allami
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 2 Pages: 143-150
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Obesity and rapid weight loss induced by weight-reducing surgery are well recognized as a risk factor for the development of gallbladder stones. There is no standard policy whether to perform prophylactic cholecystectomy at the time of the bariatric operation or to give postoperative treatment to decrease the risk.Objective: To evaluate the incidence and risk factors of gallstone formation post-bariatric surgery. The results may help to decide how to deal with and follow up patients with post-bariatric surgery.Methods: A total of 120 patients who underwent weight-reducing operations were recruited for this study. Several factors expected to influence gallstone formation were recorded such as body mass index and excessive weight loss. Study population was followed up for 12 months postoperatively. Ultrasonography examination was performed for those who developed symptoms suggesting gallstone formation.Results: Twenty-six (21.7%) patients were found to develop gallstones. Of the studied risk factors, the percentage of excess weight loss, family history and carrying allele A of the variant rs670 were significant for predicting development of gallstone post-bariatric procedures. Conclusion: Based on the results of this study, it is reasonable to put an index for the risk of developing gallstone following bariatric surgery, and according to this index, the surgeon could decide whether to perform concomitant cholecystectomy along with the bariatric procedure or do not.Keywords: Bariatric surgery, cholelithiasis, single nucleotide polymorphismCitation: Sajid H.A. Al-Helfy, Qasim S. Almayah, Risala H. Allami. Cholelithiasis following bariatric surgery: a new approach to deal with. Iraqi JMS. 2017; Vol. 15(2): 143-150. doi: 10.22578/IJMS.15.2.6


Article
Cytotoxic T-Lymphocyte Associated Antigen-4 (+49A/G) Gene Polymorphism as a Protective Factor against Toxoplasmosis

Author: Risala Hussain Allami
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 2 Pages: 240 -246
Publisher: Babylon University جامعة بابل

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Abstract

Cytotoxic T-Lymphocyte Associated Antigen-4 (CTLA-4) is a key factor in immune regulation. Polymorphisms in CTLA-4 gene may influence the status of this factor and eventually the immune response of host against the infectious agents. This response becomes of particular importance in cases when the pathogen is an opportunistic such as Toxoplasma gondii. This study aimed to explore the effect of CTLA-4+49A/G single nucleotide polymorphism (SNP) on the susceptibility to toxoplasmosis in women. Genomic DNA was isolated from 59 women with toxoplasmosis and aged matched 60 Toxoplasma-free women as controls. Tetra-Primer Amplification Refractory System-Polymerase Chain Reaction (ARMS-PCR) was used for amplification and genotyping of CTLA-4 gene using specific primers. The heterozygous genotype (AG) and G allele of the polymorphism CTLA-4+49A/G were less frequent among cases (17.12% and 16.95% respectively) than controls (40% and 35% respectively) with significant differences (OR=0.379, 95%CI=0.206-0.697, P=0.002). These data strongly suggested the protective role of CTLA-4+49G against toxoplasmosis among Iraqi women.


Article
Prevalence and Molecular Characterization of Fim H Gene in Escherichia Coli Isolates Recovered From Patients With Utis

Author: Narmin Saeed Merza
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 3 Pages: 470 -477
Publisher: Babylon University جامعة بابل

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Abstract

In this study, the prevalence of fim H gene was studied among 105 E. coli isolates obtained from urine samples of patients attended Azadi hospital in Duhok City. The intended gene was detected in94.3% of the isolates. Triplex PCR assay was applied and according to which the studied isolates were assigned into four groups namely A, B1, B2, and D groups which constituted 20.95 %, 3.8 %, 54.28 %, 20.95 %, respectively. Ten randomly selected isolates were subjected to SNPs fimH analysis with 3 reference strains of E. coli. The results revealed that 44 SNPs observed at 42 polymorphic sites accounting for 5.59%. All mutations were of substitutions and 29.5 % of mutations were transversions while transition type mutations constituted of 70.5 %. Ten SNPs accounting for 22.7 % of mutations gave rise to amino-acid changes (sense mutation) while the rest 34 (77.3 %) resulted in silent mutations. Moreover, twelve SNPs were singletons and among them five were with amino acid replacements. Amino acid replacements due to SNPs accounted for 1.27% of whole sequenced fragment of fimH.It can be concluded that there is no relationship inferred between the isolates of E.coli when the two phylotyping techniques are compared but the results of both can serve the purpose of genotypic characterization of uropathogenic E. coli.


Article
Detection of SCN1A Gene Polymorphisms in Epilepsy Children

Author: Laith Abdul Hassan Mohamed-Jawad1
Journal: Almuthanna Journal of Pure Science (MJPS) مجلة المثنى للعلوم الصرفة ISSN: 22263284 Year: 2017 Volume: 4 Issue: 2
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

Febrile seizure is a common convulsions occur in children from 5 months to 6 years of age whose sufferingfrom high temperature. A total of 50 children with febrile seizures and 50 normal control are included in this study.RFLP used to identify the A/G polymorphisms of the SCN1A gene on chromosome 2q24. Results showed that thegenotype proportions and allele frequencies for SCN1A c.3184 A/G in both groups are not significantly. Proportions ofA homozygote, A/G heterozygote, and G homozygote for SCN1A c.3184 A/G were as follows: in patients with FSs,20%, 50%, and 30%, respectively compared to controls (24%, 62%, and 14%, respectively). The allele A and Gfrequencies for SCN1A c.3184 A/G in patients with FSs was 45% and 55% respectively compared to control group. Thefrequency of AG genotype of SCN1A c.3184 A/G polymorphism was non-significant in FSs compared to control group.There was increased in glucose, phosphorus levels and decrease in sodium, chloride levels. The results do not suggestthat SCN1A p. Thr 1067 Ala or c.3184A/G (rs2298771) are susceptibility factors for febrile seizures and the fever playsan important role in causing disturbances in electrolyte balance.


Article
Tumor Necrosis Factor-Alpha Gene Polymorphisms in Iraqi patients with Chronic Periodontitis
تعدد الأشكال في جين عامل نخر الورم ألفا ((TNF-α في المرضى العراقيين المصابين بمرض النساغ المزمن

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Abstract

Background: Chronic periodontitis is an inflammatory disease of bacterial etiology that results in the destruction of tooth supporting tissues, tooth mobility, and tooth loss. The inflammatory response of the periodontal tissues to infection is influenced by environmental and genetic factors. The polymorphism of tumor necrosis factor-alpha (TNF-α) has been reported to influence the expression of TNF-α, thereby playing a role in the pathogenesis of periodontitis.Objective: To study the genotyping of tumor necrosis factor-α at position (-308) and to find out whether any associations exist between the severity of periodontitis and the gene polymorphisms.Patients and Methods: The study groups included 50 patients with chronic periodontitis and 20 healthy controls with clinically healthy periodontium with an age range of 25-50. Everyone were analyzed for polymorphism of TNF-α gene at position (-308). Periodontal parameters used in this study were plaque index (PLI), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL) and bleeding on probing (BOP). Five ml of venous blood was collected from all patients and controls. DNA was extracted from blood samples, and then the results of electrophoresis of polymerase chain reaction (PCR) products for this cytokine were subjected for sequencing and to locate the positions of possible mutations.Results: The results of sequencing for the tumor necrosis factor-α gene showed higher frequency of mutations in patient samples as compared to healthy control samples. A highly significant difference was found in the frequency of mutations among the six samples (4 patients and 2 controls) p=0.0002.Conclusion: The results of this study indicates that the (-308) polymorphism in TNF-α gene is associated with the susceptibility to chronic periodontitis.

خلفية الدراسة: مرض النساغ المزمن هو مرض التهابي من المسببات البكتيرية التي تؤدي إلى تدمير الأنسجة الداعمة للأسنان ، حركة الأسنان، وفقدان الأسنان. الاستجابة الالتهابية لأنسجة الاسنان الداعمة للاصابة تتأثر بالعوامل البيئية والوراثية.أن تعدد الأشكال لعامل نخر الورم ألفا (TNF-α) له تأثير على التعبير عن TNF-α، وبالتالي تلعب دورا في التسبب في التهاب اللثة.اهداف الدراسة : قد أجريت هذه الدراسة لدراسة التنميط الجيني لعامل نخر الورم α في موضع (-308) ولمعرفة ما إذا كان هناك أي أرتباط موجودة بين شدة التهاب اللثة وتعدد الأشكال الجيني.المرضى والطرائق : شملت مجموعات الدراسة 50 مريض لديهم التهاب اللثة المزمن و 20 الاصحاء مع لثة صحية سريريا وكانت أعمارهم تتراوح ما بين25- 50 سنة. اما فيما يخص مؤشرات ما حول الاسنان التي تم قياسها في هذه الدراسة هي (مؤشر الصفيحة الجرثومية , مؤشر التهاب اللثة , عمق جيوب اللثة , مستوى الانسجة الرابطة سريريا و مؤشر النزف عند التسبير). تم جمع خمسة مل من الدم الوريدي من جميع المرضى والضوابط. تم استخراج الحمض النووي (DNA) من عينات الدم ثم بعد ذلك أُجري الترحيل الكهربائي من منتجات تفاعل البلمرة المتسلسل (PCR) ، وأُرسلت النتائج لمعرفة التتابعات وتحديد اماكن الطفرات المحتملة.النتائج: أظهرت نتائج الكشف عن تسلسلات القواعد النتروجينية لجين عامل نخر الورم ألفا ((TNF-α تردد أعلى من الطفرات في عينات المرضى مقارنة عينات من الأصحاء. تم العثور على اختلاف كبير جدا في تردد الطفرات بين العينات الست (4 مرضى و2 الضوابط) p=0.0002.الاستنتاجات: نتائج هذه الدراسة تشير إلى أن تعدد الأشكال في الجين TNF-α في موضع (-308) يمكن أن تسهم في زيادة القابلية لمرض النساغ المزمن.

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