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Article
Spectrophotometric determination of progesterone and dopamine in breast cancer serum

Author: Safaa Sabri Najim
Journal: Univesity of Thi-Qar Journal مجلة جامعة ذي قار العلمية ISSN: 66291818 Year: 2017 Volume: 12 Issue: 1 Pages: 1-17
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Dopamine (DA) is one of the most important catecholamine neurotransmitters in the human central nervous system in the brain and plays a key role in the functioning of the renal, hormonal, and cardiovascular systems. Abnormal release of DA will contribute to some diseases such as Alzheimer’s and Parkinson's disease. Therefore, the sensitive determination of DA becomes increasingly significant in the field of clinical disease diagnosis and the research of physiological functions. Progesterone is an essential for the development and cyclical regulation of hormone responsive tissues including the breast and reproductive tract. In the breast, progesterone acts in concert with estrogen to promote proliferative and pro-survival gene programs. Progesterone has actions in breast cancer.This paper shows a spectrophotometric method for determination dopamine and progesterone concentration in serum samples, fifty six veins blood samples collected from healthy control and breast cancer patient groups. The analytical data obtained by using UV-Spectrophotometer (max = 250, 266 nm), linearity (0.5 - 2.0, 0.25 - 1.5 ng/ml) for dopamine, progesterone respectively. The molar absorptivity (), correlation coefficient (R2) and limit of detection (LOD) for dopamine, progesterone ( =2.5156  106, 4.1935 106 L.mol-1 cm-1), (R2= 0.9979, 0.99957) and (0.64, 0.57 ng/ml) respectively. The results show significant differences between the concentrations of dopamine and progesterone in control and breast cancer patient groups (p0.05).It is fast, sensitive, selective and reliable quantification spectrophotometric method used in complex biological samples.

الدوبامين هو احد اهم النواقل العصبية في الجهاز العصبي المركزي في دماغ الأنسان و يلعب دور اساس في وظيفة الجهاز ( الكلوي ، الهرموني و الوعائي القلبي). ان الإفراز غير الطبيعي للهرمون يؤدي الى امراض مثل الزهايمر ومرض باركنسون، لهذا إزدادت اهمية تقدير الدوبامين في مجال تشخيص الأمراض السريرية وبحوث الوظائف الفسيولوجية.البروجستيرون له دور اساسي في التطوير والتنظيم الدوري لإستجابة الأنسجة كالثدي والقنوات اللبنية للهرمون ، ففي الثدي يعمل مع الأستروجين لتطوير التكاثر والمحافظة على بقاء برامج الجين كما ان للبروجستيرون دور في سرطان الثدي. يوضح هذا البحث الطريقة الطيفية لتقدير الدوبامين والبروجستيرون في نماذج مصل الدم حيث جمعت 56 عينة دم وريدي لكل من مرضى سرطان الثدي واخرى للأصحاء. اوضحت النتائج التحليلية التي توفرت بأستخدام جهاز المطياف الضوئي عند الطول الموجي الأعظم (250،266) نانوميتر وخطية ضمن المدى ( 0.5-2.0 و 0.25-1.5) للدوبامين والبروجستيرون على التوالي. معامل الإمتصاص المولاري ، معامل الإرتباط وحد الكشف، ( 0.9979 ، 0.9995) و ( 0.64، 0.57) ( لتر مول-1 سم -1 4.1935  106, 2.5156 106) للدوبامين والبروجستيرون على التوالي.اوضحت النتائج وجود اختلافات معنوية بين تركيز الدوبامين والبروجستيرون في مجموعة مرضى سرطان الثدي ومجموعة السيطرة وهي طريقة طيفية سريعة وحساسة ويمكن الوثوق بها واستخدامها عند التقدير الكمي لنماذج بيولوجية معقدة.


Article
Comparative study on early pregnancy diagnosis by three different techniques in cows

Author: Baraa D. Al-Watar
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 1 Pages: 50-54
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

The aim of this study was to determine the best method, most appropriate and high precision method between rectal palpation, ultrasonography and measuring level of progesterone for diagnosis of early pregnancy in cows. 20 cows were examined in Teaching Hospital / College of Veterinary Medicine / Mosul University, at the period between 1/11/2013 - 1/3/2014, cows undergone early pregnancy diagnosis using three different methods at 45, 60 and 90 days of pregnancy. Results of rectal palpation showed that the percentage of pregnancy was 0% 60% 85% at days 45, 60 and 90 of pregnancy, respectively, while results of ultrasonography showed that diagnosis early pregnancy was 30, 80 and 100 at days 45, 60 and 90 of pregnancy, respectively. Results of estimation of progesterone concentration by ELISA technique showed that pregnancy diagnosis was 60% 85% 100% at days 45, 60 and 90 of pregnancy, respectively. In conclusion, the best and fastest way to diagnose early pregnancy in cows was measure concentration of progesterone in blood serum of cows then followed by ultrasonography, while method of rectal palpation was inefficient in detecting early pregnancy in cows.


Article
A study on plasma estradiol and progesterone profile at days 11, 12 and 13 post ovulation undergone embryo transfer at day 7-post ovulation in local Egyptian mares.

Author: Khalid Mohammed Karam
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 1 Pages: 111-115
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

This study was conducted on 28 recipient local mares after synchronizing and recovery of embryos of Arabian mares (donor mares) and transferring them to recipients on day 7 post ovulation, all mares were raised in the studs of police academy –Cairo Egypt under same circumstances in the breeding season from February till may of 2013. Seventeen recipient mares were pregnant and 9 mares were non pregnant when ultrasound pregnancy check was done on day 21 post ovulation, blood samples were taken on days 11,12 and 13 to detect the steroidal hormonal profile (estrogen and progesterone) via Elisa technique of the recipient mares plasma steroid level and its role in early pregnancy and maternal recognition. The results were significantly higher (P≤0.05) between pregnant (n=17) and non pregnant (n=9) recipient mares in the plasma progesterone concentrations which were 10.99±0.16 vs 9.59±0.11, 12.69±0.16 vs 11.79±0.22 and 14.4±0.15 vs 13.78±0.23 ng/ml on days 11,12 and 13 post ovulation respectively, significant difference(P≤0.05) was observed when comparison between pregnant mare’s plasma progesterone concentrations on days 11,12 and 13 post ovulation. Plasma estrogen concentration were significantly higher (P≤0.05) in non pregnant and pregnant mares which were15.17±0.18 vs 14.84±0.14, 14.74±0.27 vs13.94±0.12 and 14.14±0.3 vs13.12±0.16 pg/ ml on days 11,12 and respectively, on the other hand when comparison between days11,12 and 13 plasma estrogen levels were significantly different (P≤0.05) in pregnant mares while no significant difference was found in the same days between non pregnant mares, thus might be the main reason for early embryonic death when detected in early pregnancy check via ultrasonography in 21 days post ovulation.


Article
CHARACTERIZATION OF INVASIVE DUCTAL CARCINOMA (IDC) IN PRE-AND POSTMENOPAUSAL WOMEN

Author: KHALID MAHDI SALIH
Journal: karbala journal of pharmaceutical sciences مجلة كربلاء للعلوم الصيدلانية ISSN: 70272221 Year: 2017 Issue: 13 Pages: 188-196
Publisher: Kerbala University جامعة كربلاء

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Abstract

Background: Previous findings reported that the majority of BC in Iraqi women is invasive ductal carcinoma (IDC) and the most frequent subtype is estrogen receptor (ER+) and/or progesterone receptor (PR+) expressed.Objective: The present study is aimed to determine the association of age, BMI, and the levels of estradiol and progesterone hormones as well as their receptors in pre- and postmenopausal women with IDC that may ultimately help identify high-risk women who would benefit from increased screening or chemoprevention.Methodology: Forty premenopausal and fifty eight (58) postmenopausal women with IDC, previously identified their ER and PR expression, are involved in this study. Age, BMI, and serum levels of estradiol and progesterone are determined. Results: Both pre- and postmenopausal patients are presented at advanced age (45.8 ± 0.76, 59.1 ± 0.72 year) respectively. The average of BMI in both groups is within overweight category, but without significant difference between pre- and postmenopausal patients (27.3 ± 045, and 26.7±0.38 kg/m2 respectively). Both estradiol and progesterone serum levels are significantly higher in premenopausal patients (290.1± 6.5 pg/ml, and 2.55± 0.38 ng/ml respectively) than those in postmenopausal patients (264.1± pg/ml, and 0.75± ng/ml respectively). Just postmenopausal patients showed significant reverse association between E2 levels and the positively expression of ER (r= - 0.289), and PR (r= - 0.386), while progesterone levels showed significant association with age of pre- and postmenopausal, and with BMI of postmenopausal. Conclusion: Collectively, age, estradiol level, and expression of ER and PR are the main factors associated with the diversity of breast cancer in pre- and postmenopausal women and ultimately they may help in identifying high-risk women who would benefit from increased screening or chemoprevention.


Article
Study of the relationship between Toxoplasmosis disease and progesterone , Testosterone , Estradiol and Hormone Prolactin Among for abortion women in Diyala government
دراسة العلاقة بين مرض داء المقوسات على هرمونات البروجستيرون وتستوستيرون وأستراديول والبرولاكتين لدى النساء المجهضات في محافظة ديالى

Author: Aws Zamil اوس زامل عبد الكريم
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2017 Volume: 13 Issue: 1 - part 1 Pages: 1-12
Publisher: Diyala University جامعة ديالى

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Abstract

The study deal with relationship of Toxoplasmosis disease for abortion women and the level of sex hormone, the study conducted through the period of 1 march until 30 June, 84 serum sample collected to detect a specific antibodies of Toxoplasmosis Igm, IgG using the ELISA technique, samples were classified into two groups : chronic toxoplasmosis (41) and acute toxoplasmosis (24) with a (19) sample wasn’t infected used as control group,The results showed statistical analysis at the level of probability of 0.001 signifferences as hormone got Estradiol on (1147 pg/ml ) and prolactin (31.5 ng/ml) and testosterone (1.155 ng/ml) was high compared to the control, while progesterone hormone was not affected by disease and chronic toxoplasmosis and showed the results of statistical analysis at the level of 0.001 significant differences reaching progesterone (52.62 ng/ml) ievel was high and hormone Estradiol (1.357 pg/ml) is low compared to compared to control was affected by the hormone prolactin and testosterone disease acute toxoplasmosis at the level of hormones depending on age groups and found that (25-20) most affected.

تناولت هذه الدراسة علاقة داء المقوسات Toxoplasmosis بمستوى الهرمونات الجنسية لبعض النساء المصابات بهذا الداء، حيث تم الحصول على (84) عينة مصل دم من النساء المصابات وأجري لهم الكشف عن الاجسام المضادة لداء المقوسات IgM,IgG باستخدام تقنية ELISA وصنفت العينات الى مجموعتين، مجموعة مصابة بداء المقوسات المزمن Chronic Toxoplasmosis والبالغ عددهم (41) عينة ومجموعة مصابة بداء المقوسات الحاد Acute Toxoplasmosis والبالغ عددهم (24) عينة مع (19) عينة مصل دم غير مصابة كعناصر سيطرة للمقارنة، ودراسة تأثيرها على مستوى الهرمونات الجنسية. وأظهرت النتائج التحليل الإحصائي عند مستوى احتمالية 0.001 وجود فروق معنوية اذ حصل هرمون الاستيرادول على (1147pg/ml) وهرمون البرولاكتين (31.5ng/ml) والهرمون الذكري (1.155ng/ml) كان مرتفعا مقارنتا بالسيطرة بينما هرمون البروجستيرون لم يتأثر بداء المقوسات المزمن. وأظهرت النتائج التحليل الإحصائي عند مستوى احتمالية 0.001 وجود فروق معنوية اذ بلغ مستوى هرمون البروجستيرون (52.62 ng/ml) كان مرتفعا وهرمون الاستيرادول (13.21 pg/ml) منخفضا مقارنتاً بالسيطرة ، ولم يتأثر هرمون البرولاكتين والهرمون الذكري بداء المقوسات الحاد ، وتم دراسة تأثير داء المقوسات على مستوى الهرمونات اعتمادا على الفئات العمرية ووجد ان الفئة (25-20 سنة) اكثر تاثرا.

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