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Article
3.DETECTION OF NEW DELHI METALLO-BETA-LACTAMASE-1 (BLANDM-1) IN CARBAPENEM-RESISTANT PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL SAMPLES IN WASIT HOSPITALS

Authors: Zeyad K. Hussein --- Haider S. Kadhim --- Jabbar S. Hassan
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2018 Volume: 16 Issue: 3 Pages: 239-246
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Pseudomonas aeruginosa (P. aeruginosa) infections are clinical problem, it is a difficult to treat because of high resistant to many antibiotics (Multi-drug resistant) and a high risk of emergence of resistance during therapy. Carbapenems are therapeutic choice against infections caused by Gram-negative bacilli including strains of P. aeruginosa. New Delhi metallo-β-lactamase-1 (blaNDM-1) gene, an acquired class B carbapenemase. Dissemination predominantly involves transfer of the blaNDM-1 gene among promiscuous plasmids and clonal outbreaks. Bacteria with NDM-1 are typically resistant to nearly all antibiotics.Objective:To detect blaNDM-1 in the isolates of P. aeruginosa, which were recovered from various clinical samples from hospitalized patients in Wasit hospitals.Methods: This cross-sectional study involved 200 clinical samples were collected from three major hospitals in Wasit province. Samples were inoculated in Mackonkey and blood agar for primary isolation and then biochemical tests were used to confirm diagnosis of P. aeruginosa. The susceptibility test for 14 types of antibacterial drugs were tested by using disk diffusion method. Chromosomal and plasmid DNA were extracted by using special methods.Results: Out of 36 carbapenems resistant P. aeruginosa (CRPA) isolates, there were 18 isolates (50%) positive for blaNDM-1 gene.Conclusion: Rate of occurrence of blaNDM-1producers is highest among carbapenem-resistant P. aeruginosa isolated from clinical samples in Wasit hospitals. Therefore, its recognizable proof in clinical bacterial diseases will be suspected in any carbapenem resistance P. aeruginosa.Keywords: P. aeruginosa; carbapenems; metallo-β-lactamase; blaNDM-1Citation: Hussein ZK, Kadhim HS, Hassan JS. Detection of New Delhi metallo-beta-lactamase-1 (blaNDM-1) in carbapenem-resistant pseudomonas aeruginosa isolated from clinical samples in Wasit hospitals. Iraqi JMS. 2018; 16(3): 239-246. doi: 10.22578/IJMS.16.3.3


Article
Distribution of CTX-M gene among Escherichia coli strains isolated from different clinical samples in Erbil City

Author: Fattma A. Ali
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 1 Pages: 78-90
Publisher: Baghdad University جامعة بغداد

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Abstract

Escherichia coli frequently causing urinary tract, wound and blood infection resulting in significant morbidity and mortality due to had plasmid encoded ESBLs lead to failure treatment .Tow hundred samples were collected from (urine, wound, sputum), Escherichia coli. isolated and identified by using microscopical, morphological, biochemical tests and Vitek 2 compact system. Antibiotic susceptibility testing was screening according to the CLSI guideline and Vitek 2 compact system. Phenotypic screening of ESBLs was undertaken using (Double disk diffusion and Standard disk diffusion) Methods, also PCR technique was used for genotypic detection of ESBL genes (blaCTX-M) according to the standard protocol. We obtained in this study 60 (29.33%) total positive results of Escherichia coli.40 (20%) isolates for Escherichia coli isolated from urine, 15 (7.5%) wound,5 (2.5%) sputum,from patient attending e Rizgary hospital fromSemptemper 2015 to March 2016.Susceptibility profile has been done for all Escherichia coli isolated by using 13 antimicrobial agent,Our multifinding pointed out that highest resistance be Ceftriaxone 45(75%) Ceftazidime 44(73.3) Tobromycin 39(65%) Piperacillin 39(65%),most of Escherichia coli isolates were resistance to more than three antibiotics belonging to different classes used and these were considered to be multidrug resistant (MDR) isolates. The incidence rate of ESBL-producing Escherichia coli was 48 (80%) by Standard disk diffusion Method,46 (76.7%) by Double disk diffusion and 38(66.7%) of ESBL producer in urine samples. Remarkably, dissemination of blaCTX-M 44 (73.3%) genes among ESBLs-positive isolates and the length of amplified genes (550) bp for blaCTX-M genes. It can be said that the incidence rate of Escherichia coli carrying genes encoding for ESBL enzyme representing their commonness in our institute and multi resistance to many classes of antibiotic, resulting in limited treatment options.


Article
Influence of Helium-Neon Laser on Some Virulence Factors of Staphylococcus Aureus and Escherichia Coli

Author: Mohammed F. Abo-Ksour
Journal: Al-Mustansiriyah Journal of Science مجلة علوم المستنصرية ISSN: 1814635X Year: 2018 Volume: 29 Issue: 3 ICSSSA 2018 Conference Issue Pages: 29-34
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

This work aims to investigate the effect of Helium-Neon laser on locally isolated Staphylococcus aureus and Escherichia coli bacteria; their resistance pattern, virulence factors, and their plasmid content. Bacteria were collected from patient suffering from urinary tract infections, septicemia, wound, and burn infections, then isolates were identified according to their characteristics, features; morphological, biochemical tests, and API systems. Out of Eighty-seven samples, twenty-seven isolates of S. aureus and thirteen isolates of E. coli were isolated. The results showed that after laser treatment, the diameter of inhibition zone increased for almost of the used antibiotics beside some isolates became sensitive especially after 2 min of laser exposure time. The hemolysin production was disappeared in (%40) of E. coli and (%20) of S. aureus after ten min of irradiation, while it decreased to half in another S. aureus isolate. All S. aureus and E. coli isolates loosed their ability to produce β-lactamase enzymes; some of these isolates loosed it after 5 minutes and the others after 10 minutes of irradiation. The results showed that laser irradiation hasn't any effect at any exposure time on adhesion factors of both S. aureus and E. coli isolates. Plasmid profile of Irradiated E. coli illustrated disappearing of DNA plasmid bands as well as RNA after ten min of irradiation.

يهدف هذا البحث إلى دراسة تأثير ليزر الهليوم النيون على بكتريا Staphylococcus aureus و Escherichia coli المعزولة محليا, فضلا عن نمط المقاومة ، عوامل الفوعة ، ومحتوى البلازميد. تم جمع البكتيريا من مريض يعانون من التهابات المسالك البولية والتسمم الدموي والتهابات الجروح والحروق ، تم بعد ذلك تحديد العزلات وفقاً لصفاتها وبحسب نتائج الاختبارات المورفولوجية والكيميائية والاحيائية ونظام API. تم عزل سبعة وعشرين عزلة من بكتيريا S. aureus من أصل سبعة وثمانون عينة ، وعشرة عشر عزلة من E. coli. أظهرت النتائج أنه بعد العلاج بالليزر زاد قطر منطقة التثبيط للمضادات الحيوية المستخدمة تقريبا إلى جانب بعض العزلات التي أصبحت حساسة خاصة بعد 2 دقيقة من زمن التعرض للليزر. اختفى إنتاج الهيموليزين في (٪ 40) من بكتريا E. coli و (٪ 20) من بكتيريا S. aureus بعد عشر دقائق من التشعيع ، بينما انخفض إلى نصف في عزلة أخرى من بكتريا S. aureus. جميع عزلات بكتريا S. aureus و E. coli فقدت قدرتها على إنتاج إنزيمات β-lactamase. بعض هذه العزلات فقدتها بعد 5 دقائق والأخرى بعد 10 دقائق من التشعيع. وأظهرت النتائج أن التشعيع بالليزر ليس له أي تأثير على عوامل الالتصاق لكل من عزلات S. aureus وعزلات E. coli. يتضح من نتائج ترحيل البلازميد اختفاء أشرطة البلازميد DNA وكذلك RNA بعد عشر دقائق من التشعيع لبكتريا E. coli..

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