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Article
Isolation and molecular characterization of Vibrio fluvialis from diarrheal children in Nasiriyha City

Authors: Hawraa A .mohammed --- Ezat H.Mezal --- Zaman kaream hanan
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2018 Volume: 6 Issue: 4 Pages: 28-34
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

The objective of this study was to investigate the incidence, molecular features, as well as the antimicrobial susceptibilities of Vibrio.fluvialis isolated from diarrheal (children) in Nasiriya city from (July 2016 to February2017) . A total of (20) samples of Vibrio. fluvialis were collected and analyzed by bacteriological, molecular and antimicrobial methods. Green and yellow bacterial colonies were recovered on thiosulfate citrate bile salt sucrose agar plates. The phenotypic characteristics of the isolates, including morphological, physiological and biochemical traits were determined and confirmed using API 20E system. Following isolation, the 16S rRNA gene specific for the genus Vibrio.fluvailis was investigated by using PCR. Antimicrobial susceptibility to 10 antimicrobial agents was determined by the disc agar-diffusion method .In this study the isolates were more resistance to polymaxin B (70%) & streptomycin (65%) and tetracycline (60%),while doxycycline was (60%) ,ampicillin and chloramphenicol (55%) ,while trimethoprim was resistance (50%).while was more sensitative to Nalidixic acid &Azithromycin (35%)and ciprofloxacin (45%). The study showed an increase presences of Vibrio.fluvialis in diarrheal children &ahight resistance of many antimicrobial agent that importance to find suitable methods to control the infection transmission.


Article
Characterization Phenotypic using several superficial recipes for some grape cultivars (Vitis vinifera L.)
التوصيف المظهري باستخدام بعض الصفات المظهرية لعدة اصناف من العنب الاوربي

Authors: Ali Saeed Atiya Al-Janaby علي سعيد عطية الجنابي --- Hanan Ali Kareem حنان علي كريم
Journal: Kufa Journal for Agricultural Sciences مجلة الكوفة للعلوم الزراعية ISSN: 20727798 23128186 Year: 2018 Volume: 10 Issue: 1 Pages: 24-34
Publisher: University of Kufa جامعة الكوفة

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Abstract

This study was carried out at microbiology and Biotechnology laboratory Animal Production Department- College of Agriculture – AL-Qasim Green University during the period of 2015 and 2016 on ten Grape cultivars (Dis Anz, Kamali, beethalhmam, Helwany , shade beetha , shade sude , Turki , Frency , Makaoy and Salahi) Which from three different Locations Or chards (AL-hashmia – Babylon / AlboEsa and AL-manathra- Najaf ) to study the morphological description among these Grape cultivars depending of some morphological trails (leaves trails , Tandiralls, Cluster , fruiting trails , Berries trails , flowering time), Morphological description results that showed there was variation among grape cultivars where morphological similarity cluster tree that the grape cultivars were divided into two groups , First group, included Makaoy cultivar, While , the second group consists other nine grape cultivars where the similarity rate between two groups was about 22% , and high morphological similarity was between Dis Anz and Kamali reached 92.75% with less morphological distance reached 7.24% approximately . The less morphological similarity was between Dis Anz and Makaoy cultivars reached 2.67%, and with high morphological distance reached 97.33% .

اجريت هذه الدراسة في مختبر الاحياء المجهرية والتقانات الاحيائية في قسم الانتاج الحيواني – كلية الزراعة - جامعة القاسم الخضراء للموسمين 2015 و 2016 م ,على عشرة اصناف هي (ديس العنز , حلواني , شدة بيضاء , شدة سوداء , كمالي , بيض الحمام , تركي , فرنسي , مكاوي و صلاحي ) وتم جمع العينات لعشرة اصناف عنب من ثلاثة مواقع (مناطق) مختلفة قضاء الهاشمية/ بابل ,البو عيسى/ نجف و المناذرة / نجف وذلك لدراسة التوصيف المظهري بين الاصناف قيد الدراسة بالاعتماد على بعض الصفات المظهرية بين الاصناف (صفات الاوراق , نوع المحلاق , صفات العنقود الثمري , صفات الحبات , موعد الازهار) , اظهرت نتائج التوصيف المظهري وجود اختلافات بين الاصناف اذ أظهر مخطط التشابه للشجرة المظهرية توزع الاصناف الى مجموعتين رئيسيتين وضمت الاولى الصنف مكاوي اما المجموعة الثانية ضمت التسعة المتبقية وكانت نسبة التشابه بين المجموعتين 22% تقريباً , وان اعلى نسبة تشابه مظهري ظهرت بين الصنفين ديس العنز وكمالي بلغت92.75%تقريباً وبأقل بعد مظهري بلغ 7.24% كما ظهر من الشجرة المظهرية ان اقل نسبة تشابه كانت بين الصنفين ديس العنز ومكاوي بنسبة بلغت تقريبا 2.67% وبأعلى بعد مظهري بلغ 97.33% . وتطابقت نتائج جدول الاختلافات المظهرية مع نتائج مخطط التشابه للشجرة المظهرية .


Article
Synthesis and Characterization Nano Structure of MnO2 via Chemical Method

Authors: Khitam S. Shaker --- Alyaa H. AbdAlsalm
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2018 Volume: 36 Issue: 9 Part (A) Engineering Pages: 946-950
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

In the current research, Magnesium oxide II (MnO2) nanostructures were prepared by chemical route from hydro manganese chloride salt using Potassium hydroxide as reducing agent. X-Ray diffraction (XRD), SEM and Fourier transformation infrared (FTIR) used to characterize both particle size and structure of MnO2 nanoparticles. XRD results confirmed impurity of synthesized powder with α-MnO2 as predominant phase. The average particle size of manganese dioxide was in the range 25- 30 nm. Photographs of scanning electron microscope (SEM) showed two hierarchical structures, cluster agglomeration and chain appearance.


Article
Synthesis and Characterization of Iron Oxide Nanoparticles by Open Vessel Ageing Process

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Abstract

Nano-crystalline iron oxide nanoparticles (magnetite) was synthesized by open vessel ageing process. The iron chloride solution was prepared by mixing deionized water and iron chloride tetrahydrate. The product was characterized by X-Ray, Surface area and pore volume by Brunauer-Emmet-Teller, Atomic Force Microscope (AFM) and Fourier Transform Infrared Spectroscopy(FTIR) . The results showed that the XRD in compatibility of the prepared iron oxide (magnetite) with the general structure of standard iron oxide, and in Fourier Transform Infrared Spectroscopy, it is strong crests in 586 bands, because of the expansion vibration manner related to the metal oxygen absorption band (Fe–O bonds in the crystals of iron oxide). The results show that the prepared nano iron oxide is with average crystal size 75.92 nm, surface area was 85.97 m2/g and the pore volume was found equal to 0.1566 cm3/g.


Article
Biodegradable Plastic Production by Bacillus spp. Isolated from Agricultural Wastes and Genetic Determination of PHA Synthesis

Author: Neihaya H. Zaki
Journal: Al-Mustansiriyah Journal of Science مجلة علوم المستنصرية ISSN: 1814635X Year: 2018 Volume: 29 Issue: 1 Pages: 67-74
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Polyhydroxyalkanoates (PHAs) are resources that hoard by a variety of microorganisms as a nutrient source. It is an environmentally recyclable biopolymer, and totally corrupted biologically to simple molecules. A total of 38 isolates from agricultural wastes, 9 isolates exhibited optimistic outcome (dark blue particles) for PHA accumulation by Sudan black B staining method. Maximum bacterial growth was achieved at temperature 35°C, PH= 7, for 48 hr. incubation and 0.25% concentration of NaCl. Chosen the isolate (NE1MOK) recognized as Bacillus subtilis when diagnosed by traditional and molecular methods by amplification 16s rRNA gene. Bacillus subitilis had an ability to accumulate 0.26 g/l (40.625 %) of PHA, and characterized by Fourier transforms infrared (FTIR) analysis, which exhibited functional groups of PHA as (C-O, C=O, CH2, CH3, C-O-C, C-H and terminal OH). The responsible gene was detected by PCR, and the results of amplification were shown in all isolates with a length of amplicons 505 bp. The aim of this study was to detect more efficient bacterial isolate that produce PHAs from agricultural wastes in optimal conditions, and determining the genes responsible for it.

بولي هيدروكسي الكانواتس (PHA) من المواد التي تستخدم من قبل مجموعة متنوعة من الكائنات الحية الدقيقة كمصدر للمغذيات. وهو بوليمر قابل لإعادة التدوير بيئيا، ويتحلل تماما بيولوجيا لجزيئات بسيطة. من مجموع 38 عزله من المخلفات الزراعية ، 9 عزلات اظهرت نتيجة واضحة (جزيئات زرقاء غامقة) من تراكم PHA عند صبغها بطريقة التصبيغ بالسودان السوداء. ظهر اعلى نمو للبكتريا عند درجة حرارة 35م، اس هيدروجيني 7، حضن لمدة 48 ساعة و 0.25٪ من تركيز كلوريد الصوديوم. عرفت العزلة المختاره والمسماة (NE1MOK) على انها عصية Bacillus subtilis عندما تم تشخيصها بواسطة الطرائق التقليدية والجزيئية عن طريق التضخيم لجينات 16 Sالرايبوسي. وكانت Bacillus subtilis قادرة على تراكم 0.26 غرام / لتر (40.625٪) من PHA ، والذي شخص بجهاز مطياف الأشعة تحت الحمراء واظهر مجاميع وظيفية للـ PHAمثل (C-O، C=O، CH2، CH3، C-O-C ، C-H و OH). تم تعيين الجين المسؤول بالـ PCR وكانت النتائج إيجابية لجميع العزلات وكان طول الامبليكونس 505 زوج قاعدي. ان الهدف من هذه الدراسة هو تحديد العزلة البكتيرية الكفؤه لانتاج الـ PHA من النفايات الزراعية في ظروف مثلى، وتحديد الجينات المسؤولة عنه .


Article
MICRO-STRUCTURAL CHARACTERIZATION OF CEMENT STABILIZED TROPICAL lATERITE CLAY SOIL CONTAMINATED BY HEAVY METALS

Authors: Dr. Khitam Abdulhussein Saeed --- Shaymaa Abdul Muttaleb Hashim
Journal: Journal of Engineering and Sustainable Development مجلة الهندسة والتنمية المستدامة ISSN: 25200917 Year: 2018 Volume: 22 Issue: 2 (Part-2) Pages: 134-145
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

The presence of contaminated soils due to industrials and mining activities is a major concern in today’s heavily industrialized world. The contaminants lead to poor engineering properties for these soils. In this study 10% cement is used to stabilize laterite clay soil contaminated by heavy metals of Cu and Zn. However, the effect of these contaminants on the geotechnical properties of clayey soils can be altered through chemical stabilization using traditional stabilizers like cement, which result in achieving suitable material for construction purposes. The micro-structural characterization of the cement-treated Laterite clay is presented in this research. The changes in the mineralogy and morphology structure due to the effect of stabilizer of cement and heavy metals were discussed based on X-ray Diffractometry (XRD), and Field Emission Scanning Electron Microscopy (FESEM). According to the micro-structural characterization of the stabilized soils, cement was effective stabilizer in terms of improving the strength of contaminated treated soils. However, the heavy metals have retarded effect on the cement treated samples. This was due to precipitation of the metals onto the surface of calcium and aluminium silicates as insoluble hydroxides or sulphates. Thus, these compounds form an impermeable coating that acts as a barrier to inhibit cement hydration by impending transport of water into cement grain. Finally, it can be concluded that the mechanical and the physico-chemical behaviours of the compacted specimens, as determined during testing, formed the basis for evaluating both the degree of immobilization of the heavy metal in the soil matrix, and the potential for rehabilitation of contaminated sites.


Article
Hydrodesulfurization and Hydrodearomatization of Kerosene over high metal loading Ni w/γ-Al2O3 Catalyst
ازالة الكبريت والمركبات العطرية بالهيدروجين من الكيروسين باستخدام عامل مساعد NiW/γ-Al2O3 ذو نسبة تحميل عالية

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The present research investigates hydrodesulfurization (HDS) and hydrodearomatization (HAD) of raw kerosene with 0.364 wt. % sulfur and 16.498 wt. % aromatics supplied from Al-Dura Refinery. Hydrotreating reactions were carried out in one stage reactor over high metal Ni W/γ-Al2O3 prepared catalyst at different temperatures 275-350oC, pressures 32-62 bar, LHSV 1-4 hr-1, and H2/HC ratios 200-500. The prepared catalyst was pre-sulfided remarkably at low temperature and high pressure to avoid risky of sintering associated with gaining the active sulfide phase at high temperatures. Results showed an obvious differences between HDS and HAD rates due to difficulty of polyaromatics saturation compared with desulfurization. Sulfur and aromatics removal were achieved from 74.9% to 95.6% and from 1% to 12.8% respectively at different operating conditions. Advanced saturation conditions were promoted the hydrogenation reaction bath and played a significant role for achieving acceptable levels of HDS and HDA. In general, HDS and HDA increased with decreasing LHSV and increasing temperature, pressure, and H2/HC ratio. Burning quality, fire hazard, and aromatics content of kerosene were characterized by measuring of smoke point, flash point, and aniline point respectively. These properties were enhanced at different hydrotreating conditions due to the skeletal conformation change of kerosene hydrocarbons during reactions.


Article
Activity of Peroxidase in Free Radicals,Human Blood Cells and Effect of the pH and Temperatureon Peroxidase Activity and Stability

Authors: Karrar R. Mohammed --- Entesar H. Ali
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2018 Volume: 26 Issue: 9 Pages: 108-115
Publisher: Babylon University جامعة بابل

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Abstract

The previously studiesof enzymes were focused only on the extraction and purification of enzyme and increase the toxic effect of drugs and supplement of free radical scavenging. So,the aim of thestudy wasto extend purified peroxidase from Pseudomonas aeruginosa application and to abundant new enzymatic drug of free radicals scavenging with low side effect. Peroxidase is categorized as oxido-reductases, whichwere used for catalyzing various oxidative reactions that protect cells from the harm effect of free radicals.Peroxidase was tested as antioxidant and test their toxicity on human cells to overcome the problem of the side effect of drug and supplement that used as an antioxidant. The enzyme was applied as H2O2 scavenging activity antioxidant by used three concentration of enzyme and triplicate (10, 40, 60 µg/ml).The enzyme was show higher activity at 60 µg/ml reached to 40% activity. The cytotoxicity of peroxidase was tested on whole healthy human blood by C.B.C by using equal amount 5µg of (enzyme substrate, only substrate and enzyme without substrate). The result showed that the enzyme only had no effect on human blood as compare with H2O2 and enzyme with H2O2. The characterization of enzyme was done by using more than one temperature and pH value and the result were shown that the optimal temperature for enzyme activity and stability rang (40-45°C) and the optimal pH for enzyme stability and activity were 7.

تركزت الابحاث السابقة للانزيمات على استخلاص وتنقية الانزيمات والمؤثرات الجانبية لاستخدام الادوية والمكملات الغذائية الكابحة للجذور الحرة. لذلك، الهدف من الدراسة هو توسيع تطبيقات إنزيم البيروكسيديز المنقى من الزوائف الزنجارية والقدرة على اكتشاف علاج انزيمي متوفر ورخيص الثمن وقليل التاثيرات الجانبيه. انزيم البيروكسيديز من الانزيمات المؤكسدة المختزلة والتي تعمل على تحفيز العديد من تفاعلات الاكسدة لتخليص الخلايا من خطر الجذور الحرة. اختبر البيروكسيديز في قدرته على كبح الجذور وسميته على خلايا الدم للإنسان لحل مشكلة الادوية والمكملات الغذائية. لذا استخدمت ثلاث تراكيز لانزيم البيروكسيديز(10,40,60 مايكروغرام/مل). حيث اظهر البيروكسيديز فعالية عالية في كبح الجذور الحرة عند تركيز (60 مايكروغرام/مل) وصلت الى 40.%درس تاثير الخلوي السام للبيروكسيديز على جميع خلايا الدم للانسان باستخدام جهاز عد الخلايا وباستخدام كميات متساوية من (الانزيم,المادة الاساس,الانزيم مع المادة الاساس) حيث لم يظهر الانزيم بدون المادة الاساس سوى تغيرا طفيفا باعداد الخلايا مقارنة بالمادة الاساس والمادة الاساس والانزيم. توصيف انزيم البيروكسيديز أجرى باستخدام أكثر من درجة حرارة واحدة وأكثر من اس هيدروجيني واحد حيث اظهرت النتائج ان أفضلاس هيدروجيني لفعالية الانزيم وثباتيته كانت عند اس هيدروجيني 7 وافظل درجة حرارة عند (40-45) درجة مئوية.


Article
Synthesis and Characterization of Two 3,4-Dihydropyrimidinone Derivatives
تخليق وتشخيص بعض مشتقات ]3و4-داي هايدروبرميدين[

Authors: Noor Raisan Ali نور ريسان علي --- Mahmood Shakir Magtoof محمود شاكر مكطوف
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2018 Volume: 14 Issue: 4 Pages: 170-175
Publisher: Diyala University جامعة ديالى

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Abstract

In this study we have successfully synthesis and characterization two of [3,4-dihydropyrimidin-2-(1H) ones] (1a and 1b). The compounds were synthesized by reaction of ethyl acetoacetate and aromatic aldehyde with urea in the presence of ethanol and FeCl3.6H2O with 3-5 drops of HCl at 60-65OC. Spectral measurements (e.g. FT-IR,1H-NMR and 13C-NMR) are used to investigate the chemical structures and characterize the obtained product of synthesized compounds.

في هذه الدراسة نجحنا في تحضير وتشخيص بعض مركبات 3 و 4- داي هايدروبرميدين [1a,1b] حيث حضرت هذه المركبات من تفاعل ايثل استواستيت والالديهايدات الاروماتيه مع اليوريا بوجود الايثانول وكلوريد الحديديك سداسي الماء و3-5 قطرات من حامض الهيدروكلوريك في (60-65) درجه سليزيه. مختلف القياسات الطيفيه (e.g.FT- IR, 1H-NMR, 13C-NMR) استخدمت للتحقيق وتميز المركبات التي تم الحصول عليها.


Article
Synthesis and Characterization of new 3-(2-(6-oxo-1,3-thiazinan-3-yl)-R)-1,3-oxazepane-4,7-dione and N-Bromo Amines 1,3-oxazepane-4,7-dione Derivatives.
تحضير وتشخيص بعض مشتقات 3-(2 -(6-- ثيازينان -3- يل)- R ) -3,1-اوكسازبان7,4- دايون و -N - بروموامين -3,1- اوكسازبان -7,4- دايون الجديدة .

Authors: Safa Abdulsalam Abdalgabar صفاء عبد السلام --- Walid Faraj AL-Hiti وليد فرج حمادي
Journal: Journal of university of Anbar for Pure science مجلة جامعة الانبار للعلوم الصرفة ISSN: ISSN: 19918941 Year: 2018 Volume: 12 Issue: 1 Pages: 66-74
Publisher: University of Anbar جامعة الانبار

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Abstract

This study includes synthesis and characterization of new derivatives of 3-(2-(6-oxo-1,3-thiazinan-3-yl)-R)-1,3-oxazepane-4,7-dione and N-Bromo amines 1,3-oxazepane-4,7-dione derivatives. Schiff's bases reactions through one step process in inert solvents. Some employing Schiff's bases [1- 4]; in addition, synthesized by the reaction of different amines with (Salicylaldehyde) in absolute ethanol under reflux. Heterocyclic rings of 1,3-oxazepane-4,7-dione prepared by the reaction of succinic anhydride with Schiff's bases [1-4] and 3-(2-(6-oxo-1,3-thiazinan-3-yl)-R)-1,3-oxazepane-4,7-dione derivatives were prepared by the reaction of 3-mercaptopropanoic acid with 1,3-oxazepane-4,7-dione[A1-A4] in 1,4-dioxan. Synthesis of some N-bromo amine derivatives by the reaction of 1,3-oxazepane-4,7-dione[A1-A4] with 2,4,4,6-TBCD (2,4,4,6-tetrabromocyclohexa-2,5-dienone) in dry benzene; The prepared compounds were identified by melting point, FT-IR, UV-Vis and 1H- NMR spectroscopy.

تضمنت الدراسة تحضير بعض المشتقات 3-(2-(6- اوكسو -3,1- ثيازينان-3- يل) -R-)-3,1- اوكسازبان -7,4- دايون , N- بروموامين -3,1- اوكسازبان -7,4- دايون. من تفاعل قواعد شيف الثنائية الامين (4-1 ) المحضرة من تفاعل الامينات الثنائية مع السلسلديهايد في الايثانول المطلق والتصعيد لمدة ساعتان .حضرت المركبات الحلقية الغير متجانسة 3,1- اوكسازبان -7,4- دايون من تفاعل انهيدريد السكسنيك الحلقي مع قواعد شيف( 4-1) ومشتقات 3-(2-(6-اوكسو -3,1- ثيازينان -3- يل)-R) ,3- اوكسازبان-7,4- دايون . من تفاعل 3- مركبتو حامض البروبانويك مع مشتقات 3,1- اوكسازبان 7,4- دايون (B1-B4) في 4,1- دايوكسان كمذيب .حضرت مشتقات N- بروموامين (C1- C4) من تفاعل 3 ,1- اوكسازبان -7,4 - دايون (A1-A4) مع ( 6, 4, 4, 2- رباعي بروموسايكلو هكسا - 5, 2 - دايون ) في البنزين الجاف .شخصت جميع المركبات الحلقية الغير متجاسة المحضرة بواسطة درجة الانصهار ,طيف الاشعة فوق البنفسجية ( U-Vis) ,الاشعة تحت الحمراء(FT-IR) و طيف الرنين النووي المغناطيسي (1HNMR) .

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