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Article
Estimate Antimicrobial activity and Anti-biofilm formation of bark Cinnamomum zeylanicum on Klebsiella pneumoniae isolated from Urinary Tract Infections

Author: Enass Ghassan Sweedan
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 3C Pages: 1560-1566
Publisher: Baghdad University جامعة بغداد

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Abstract

Five isolates (25%) of Klebsiella pneumoniae were isolated from urine samples. In addition also isolated bacteria were (10) 50% Escherichia coli, while (3)15% Proteus spp., (2)10% Pseudomonas aeruginosa. The ethanolic extract of Cinnamomum zeylanicum bark were tested against Klebsiella pneumoniae by using the well agar diffusion test, the alcoholic bark extract from (200 -12.5) mg/ml possessed antimicrobial activity against tested microorganism. At 200 mg/ml, and 100 mg/ml concentrations was diameter of inhibition zone rang from (18-26mm), (14-16mm) respectively, and these results compared to antibiotics Norfloxacin(10µg) inhibition zone (24-30mm), and Cefotaxim (10 µg) (26-27mm) as control. Antibiotics sensetivity were used ten antibiotics with disc diffusion method against K. pneumoniae, they were sensitive to Cefotaxime, Imipenem /Cilactin,Sparfloxacin, and Norfloxacin antibiotics only, and were resisted to the others antibiotics. Minimum inhibitory concentration was tested for plant extract, and was ranged versus Klebsiella pneumoniae (12.5-25) mg/ml. It could be concluded that alcoholic extract of Cinnamomum zeylanicum had a good antimicrobial effects and anti-biofilm on Klebsiella pneumoniae with significant difference (p< 0.05). This caused a decrease in the biofilm of the tested bacteria. Hence, it may be used instead of antibiotics to treat UTIs caused by bacteria used in current study.


Article
Molecular detection of SHV-Type ESBL in E. coli and K.pneumoniae and their antimicrobial resistance profile
الاكتشاف الجزيئي لـ ESV-Type ESBL في E. coli و K.pneumoniae ومظهر مقاومتهما المضادة للميكروبات

Authors: Ahang Hasan Mawlood --- Sharmin Abdullah Omer --- Salah Tofik Jalal --- Sayran Hamad Haji
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2018 Volume: 22 Issue: 2 Pages: 262-272
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: The increase in the incidence of Beta-lactam resistance in Gram-negative bacteria has become a main clinical problem worldwide that limits therapeutic options. The production of extended-spectrum blactamases is the major source of resistance to newer beta-lactam antibiotics in Enterobacteriaceae. This study aimed to check the presence of extended-spectrum β-lactamase producing K. pneumoniae and E. coli and the evaluation of ESBL among those isolates.Methods: A total of 120 E. coli and K. pneumoniae clinical isolates were obtained from Rizgary Teaching Hospital in Erbil City, Iraqi Kurdistan Region, during the first six months of 2016. All isolates were identified and checked for the production of ESBL using Vitek 2 automated system. The technique of PCR was performed for the detection of the presence of blaSHV gene from these isolates. The antibiotic resistant profiles for these isolates were also investigated.Results: The overall proportion of ESBL-producing E. coli and K. pneumoniae isolates, 77 (76.2%) and 15 (78.9%) isolates were ESBL producers, respectively. ESBL-producing isolates were significantly more resistant than Non-ESBL-producers (P < 0.05). PCR performed on 30 ESBL positive isolates, 21(70%) isolates were of E. coli, and 9 (30%) isolates were of K.pneumoniae. The ESBL B-lactamase related SHV gene was detected only in 11 (36.6%) isolates, 6 (28.5 %) of E. coli and 5(55.5%) of K. pneumoniae isolates respectively. All isolates were susceptible to carbapenems.Conclusion: The great diversity of ESBL and the prevalences of clinical isolates of E. coli and K. pneumoniae producing these enzymes indicate that this is an important problem in our region. The most active antimicrobial agent against isolates used in this study was carbapenem. It is, therefore strongly recommended to consider carbapenems as the drug of choice for such multi-drug resistant ESBL-producing microorganisms.

Keywords

ESBL --- blaSHV gene --- E. coli --- K. pneumoniae


Article
Detection of Extended Spectrum β–Lactmase GeneeCTX-M-1 in Escherchia coli and Klebseilla pneumonia Isolated from Urinary Tract Infection of Pregnant Women in Al-Nassyriah City

Authors: Nasser Saleh Lhwak --- Yahya A.Abbas
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2018 Volume: 6 Issue: 4 Pages: 92-96
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

This study was performed to detect the presence of CTX-M-1 gene in Klebseilla pneumonia and Escherchia coli are that causing urinary tract infections in pregnant women in Al- Nassyriah city , during the period from september (2016) to february (2017).Three hundred and thirty (n= 330) urine samples were collected and cultured on appropriate media for initial isolation. Morphological, conventional biochemical tests and API 20 system were performed to identify the pathological bacteria agents.Ninety samples showed positive culture, the most common isolates were E. coli 57 (63.3%) followed by K. pneumonia 21(23.3%) and 12(13.3%) from other Gram negative bacteria.ESBLs gene CTX-M-1(688 bp) was found in 22 isolates 17/57 (29.8%) in E.coli and 5/21 (23.8 %) in K. pneumonia.

Keywords

ESBLs --- E.coli --- K.pneumoniae --- Pregnant Women --- UTI

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