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Article
DIRECT EFFECT OF RED LASER IRRADIATION ON TESTICULAR AND EPIDIDYMIS TISSUE FUNCTION IN MALE RABBITS
التأثیر المباشر لإشعاع اللیزر الأحمر على انسجة الخصیة والبربخ في ذكور الأرانب

Author: Ihsan Ali Habeb*, Zainab Waheed* * ,Mosa F. Abbas إحسان علي حبیب *، زینب وحید **، موسى فاضل عباس
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2018 Volume: 17 Issue: 2 Pages: 42-50
Publisher: Basrah University جامعة البصرة

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Abstract

This study was designed to evaluate the effect of red laser irradiation on testicular andepididymis tissue. Twenty rabbits, male mature were used in the present study. Theanimals were divided in to four groups. First group as control and second, third and fourthgroups exposed to red laser irradiation 5 min, 15 min, 30 min respectively. Histologicalsample were taken after necropsy method of all experimental animals for preparation slideprocesses to examine under light microscope. The result of experiment showedhistological changes induced by exposure the animals to red laser (650nm-10w) whichpresent as versus changes and these pathological changes is directly fit with period ofexposure which appear as degeneration, odematous, hyperemia and necrosis in totesticular and epididymis.

صُممت ھذه الدراسة لتقییم تأثیر اشعاع اللیزر الأحمر على أنسجة الخصیة والبربخ. تم استخدام ٢٠منالأرانب الذكور الناضجة في الدراسة الحالیة. تم تقسیم الحیوانات إلى أربع مجموعات، المجموعة الأولى كسیطرة و ٤ ،٣ ،٢مجموعات تعرض للإشعاع باللیزر الأحمر ٥دقائق و ١٥دقیقة و ٣٠دقیقة على التوالي یومیا لمدة ٣٠یوم. تم أخذ عینة نسیجیة بعد تشریح جمیع حیوانات التجربة لتحضیر شرائح نسیجیة لفحصھا تحت المجھر الضوئي.نتیجة التغیرات النسیجیة الناتجة عن تعرض الحیوانات إلى اللیزر الأحمر ) ٦٥٠نانومتر - ١٠واط( والتي تظھر فيمقابل التغیرات ، وھذه التغیرات المرضیة تتناسب مباشرة مع فترة التعرض التي تظھر على شكل احتقان أو فقردموي أو نخر في التراكیب النسیجیة للخصیة والبربخ


Article
The Effects of different type of cryopreservation on DNA in testicular tissue of mice

Author: Hakeem JK
Journal: Muthanna Medical Journal مجلة المثنى الطبية ISSN: 2226146x Year: 2018 Volume: 5 Issue: 2 Pages: 115-134
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures, such as (typically) -80°C or -196°C and this process use extensively in programs of in vitro fertilization (IVF). This study is an attempt to evaluate DNA fragmentation in the cell after cryopreservation/thawing cycle when using different types of cryoprotectant (CPA), sixty mature fertile male mice were used in the current study, the mean age of these mice were ten weeks. Fifteen of them were considered as control group and the rest (forty five) as cryopreserved group, this group was divided into three subgroup according to the type of cryoprotectant (glycerol, 1, 2 propanediol and dimethylsulfoxide), each subgroup composed from fifteen mice. The testes were cryopreserved for six weeks then DNA fragmentation assay was done by single cell gel electrophoresis (comet assay). Comet results of the present study showed a highly significant (P<0.0001) increase in DNA damage in the cryopreserved testis (33.26%, 38.8% and 30.6% represent cryoprotectant glycerol, 1, 2 propanediol and dimethylsulfoxide respectively) compared with control group (23.06%) after six weeks of cryopreservation. From the results of the present study, it was concluded that there was increase the levels of DNA fragmentation in the testicular tissue after cryopreservation /thawing cycle differs according to the type of cryoprotectant, Dimethylsulfoxide cryoprotectant provides good protection to the testicular tissue and DNA in cryopreservation /thawing cycle than glycerol and 1, 2 propanediol.

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