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Article
Salivary vitamin E and uric acid in patients with OLP and healthy individuals

Authors: Ameena Ryhan Diajil --- Adel Jasim Mohammed
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2019 Volume: 31 Issue: 3 Pages: 39-43
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Oral Lichen planus (OLP) is a T-cell mediated chronic inflammatory oral mucosal disease of unknown etiology. Recent studies have reported an increased oxidative stress and lipid peroxidation in such patients. This suggests that reactive oxygen species may have a role in the pathogenesis of lichen planus. Oxidative stress in OLP release molecules consisting of granzymes resulting in local tissue damage in the effectors.Antioxidants that can defend against oxidative stress in the body cells include enzymes, as well as non- enzymatic antioxidants, such as melatonin, uric acid, vitamin A and E.Purpose: To study the level of salivary vitamin E and uric acid as antioxidant agents in patients with OLP and compared with healthy control.Methods: Twenty five patients with OLP were enrolled in this study. Age, gender, occupation, smoking status (smokers or non-smokers), lesion types, duration, location and size were recorded for each patient. After an oral examination, salivary samples were collected and flow rates (ml/min) were recorded. The collected samples were centrifuged at 3000 rpm for 10 minutes; the clear supernatants were separated and stored frozen at (-20 c) until analysis. Then salivary vitamin E was investigated using ELISA kit based on bioten double antibody sandwich technology .Uric acid was analyzed using a proprietary enzymatic reaction mixture that enables the detection of uric acid by the production of a red chromogen, which is quantitatively measured at a wavelength of 515 or 520 nm.Results: The mean age of OLP patients was 48.3 years with a range of 30-60 years. Control group consisted of 35 healthy subjects who were age matched with OLP patients. Fourteen (56%) patients were with reticular and 11 (44%) were with erosive form, with the buccal mucosa was the most commonly affected site (88%), followed by tongue (8%) then gingiva (4%). A significantly lower salivary flow-rate, lower salivary vitamin E and uric acid level in OLP patients compared to control; while, no significant difference was seen between reticular and erosive type of OLP for both vitamin E and uric acid level. Regarding gender, no differences were found between males and females in salivary vitamin E. No significant correlation was found between vitamin E /uric acid and age. Similarly, no difference was found between males and females in uric acid.Conclusion: Salivary anti-oxidant markers represented by vitamin E and uric acid decreased in OLP patients due to increase oxidative stress which may have an important role in the pathogenesis. Thus, it is recommended to give OLP patients anti-oxidant agents that may either help in healing process or decreased the severity.

Keywords

OLP --- Saliva --- vitamin E --- uric acid..


Article
Estimation and Evaluate of uric acid , Cholesterol and Triglycerides Levels in Hypertension patients in Basra Governorate – Iraq

Authors: Haneen S. Khalil --- Sahera Gh. Sayyah
Journal: Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) ISSN: 18172695 Year: 2019 Volume: 45 Issue: 1A Pages: 32-44
Publisher: Basrah University جامعة البصرة

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Abstract

The study included the measurement of the mean serum Uric acid, Cholesterol, Triglycerides levels in hypertension patients in Basra Governorate – Iraq. In this study a total number of (80) hypertensive patients (41 males, 39 females) classified according to (age, sex, family history, severity and smoking) as compared with (60) healthy (34 males, 26 females) subjects were investigated as control group. It was found that a highly significant increase is in uric acid, triglycerides and cholesterol in the blood of hypertensive patients as compared with healthy subjects. In accordance with age, severity, family history and smokers. There were significant changes in these variables between males and females. In conclusion hypertensive patients suffer a high degree of reactive oxygen species (ROS) formation causing considerable oxidative stress indicated a high levels of the serum uric acid, triglycerides and cholesterol


Article
Effect of Oral Supplementation for Rabbits of Pomegranate seed Extract on Some Serum Biochemical Parameters in Relation to oral Inflammation, oxidative stress, and wound healing

Authors: Rezan Majeed Omer --- Dilyar Ahmed Baban, --- Bakhtiar Mohyadeen Ahmed
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2019 Volume: 31 Issue: 2 Pages: 14-19
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Pomegranate (punicagranatum L, Punicaceae), is an edible fruit consumed around the world. The edible part of pomegranate is rich in compounds that possess antioxidant and anti-inflammatory activities. The aim of this study is to investigate the antioxidant; anti-inflammatory and gingival wound healing effects of Punicagrantum L. seed extract oral supplementation in rabbit. Methods and Methods: Forty five male rabbits were divided into 3 groups, base line (5 rabbits) left without buccal gingival wound as( group 1),study group, 20 rabbits (group2) with buccal gingival wound treated with ethanolic extract of Punicagranatum L. seed extract and control, 20 rabbits (group 3) with buccal gingival wound only. Buccal gingival wounds were created on lower right central incisor and sutured removed after (7) days. Blood biopsies by cardiocentesis were collected at times (0, 3h, 1, 3 and 7days) for estimation of serum alkaline phosphatase activity, serum total proteins, and serum uric acid. Results: The results showed a significant increase in serum Alkaline phosphatase, total proteins and uric acid in all time intervals after buccal gingival wound, in rabbits receiving water, while their levels increased significantly only at time intervals of 3 hours and 1 day after gingival wound, in rabbits receiving pomegranate seed extraction. Conclusions: It has been concluded that oral Supplementation of pomegranate seed extract in rabbits can expedite the rate of healing of gingival wound.


Article
Assessment of Serum Uric Acid and Highly Sensitive C-reactive Protein in Patients with Acute Respiratory Distress Syndrome
تقييم تركيز حمض اليوريك وبروتين سي التفاعلي عالى الحساسية في امصال المرضى الذين يعانون من متلازمة الضائقة التنفسية الحادة

Authors: Azheen A.M. Aljmor ازين علي --- Dawood S.H. Alazawi داود سلمان --- Abdulrazak SH.Hasan عبدالرزاق شفيق
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2019 Volume: 32 Issue: 1 Pages: 7-12
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Abstract: Background: Acute respiratory distress syndrome (ARDS) is characterized by the acute onset of pulmonary edema of non-cardiogenic origin, along with bilateral pulmonary infiltrates and reduction in respiratory system compliance with refractory hypoxemia that is the hallmark of the syndrome. Objectives: This study is designed for the assessment of serum uric acid (SUA)and highly sensitive C-reactive protein (HSCRP) in patients with ARDS and for the determination of risk factors promotes the induction of ARDS.Subjects and methods: This case control study was conducted in Al-Batool Teaching Hospital for Maternity and Children for the period from August 2017 to June 2018. 200 subjects were included, 100 patients who were admitted to neonate intensive care unit with age range 1-2 days. Another 100 age and sex matched apparently healthy infants were included as control group. A specific questionnaire form was preconstructed for this purpose. Blood samples were collected from subjects. Sera were separated and the determination of serum uric acid was carried out by (COBAS INTEGRA systems, Germany) The determination of highly sensitive C-reactive protein was measured by (COBAS INTEGRA systems, Germany) Human privacy was respected by taken patient's parents consent. Furthermore, the study was approved by the Ethical Committee in the College of Medicine.Results: The results revealed that the mean ± SD of serum uric acid in patients was significantly higher than that of controls (328.52 ± 151.19 versus 264.15 ± 85.12, t-test = 3.71, P = 0.001). Similarly, the mean ± SD of highly sensitive C-reactive protein titer was significantly elevated in neonates with respiratory distress syndrome versus controls (5.19 ± 16.11 versus 1.3 ± 1.7, t-test = 2.398, P= 0.017). The SUA was significantly higher in patients weighted less than 2.5 Kgs (t-test = 2.688, P= 0.008). Furthermore, preterm patients had significantly higher SUA (t-test = 2.435, P = 0.017). The results also found that patients who required medical intervention had higher levels of SUA (t-test = 2.395, P = 0.024). Additionally, patients who were dead had significantly higher titer of SUA (t-test = 8.545, P = 0.001). While other factors including gender and age showed insignificant effect. Concerning the HSCRP, preterm patients had significantly higher titers versus term patients (t-test = 2.066, P = 0.042). However, other factors including gender, age, weight, intervention required and final outcome were failed to reach the levels of statistical significance.Conclusion: The study concluded that the serum uric acid concentration and the highly sensitive C-reactive protein titer can be employed as diagnostic adjuncts for RDS in newly born infants, and certain patient's and mother's features were significantly associated with these markers.

تمهيد: تتميز متلازمة الضائقة التنفسية الحادة (ARDS) بالظهور الحاد لوذمة رئوية غير قلبية المنشأ ، جنبا إلى جنب مع الارتشاح الرئوي الثنائي وتدني كفائة الجهاز التنفسي مع نقص الأكسجة التي هي السمة المميزة للمتلازمةالاهداف: تم تصميم هذه الدراسة لتقييم تركيز حمض اليوريك (SUA) وبروتين سي التفاعلي الحساس للغاية (hsCRP) في امصال المرضى الذين يعانون من ARDS ولتحديد عوامل الخطورة التي تعزز من حدة متلازمة الضائقة التنفسية الحادة.المجاميع وطرق العمل: أجريت هذه الدراسة الشاملة المستعرضة في مستشفى البتول التعليمي للأمومة والطفولة للفترة من اب 2017 حتى تموز 2018. تم شمول 200 شخص في هذه الدراسة ، 100 مريض تم إدخالهم إلى وحدة العناية المركزة للمواليد الجدد (NICU) من الفئة العمرية 1-2 يوم، و 100 اخرين بنفس العمر من حديثي الولادة بصحة جيدة كمجموعة ضابطة. تم إعداد استمارة استبيان خاصة لهذا الغرض. تم جمع عينات الدم من المجاميع. تم فصل المصل وتم قياس تركيز حمض اليوريك في الامصال بواسطة (أنظمة COBAS INTEGRA ، ألمانيا)0 تم قياس تركيز البروتين التفاعلي عالي الحساسية hsCRP بواسطة (أنظمة COBAS INTEGRA ، ألمانيا) تم احترام الخصوصية البشرية عن طريق الحصول على موافقة الوالدين للمريض. علاوة على ذلك ، تمت الموافقة على الدراسة من قبل اللجنة الأخلاقية في كلية الطب.النتائج: أوضحت النتائج أن متوسط ± الانحراف المعياري SD لتركيز حمض اليوريك في امصال المرضى كان أعلى بشكل معنوي مقارنة بالمجموعة الضابطة (328,52 ± 151,19 مقابل 264,15 ± 85,12 ، t-test = 3.71، P = 0.001). وبالمثل ، فإن متوسط ± الانحراف المعياري لعيارية بروتين سي التفاعلي عالي الحساسية hsCRP كان مرتفعًا بشكل معنوي عند المرضى مقارنة بمجموعة السيطرة (5,19 ± 16,11 مقابل 1,3 ± 1,7 ، t-test = 2.398 ، P = 0.017). كان تركيز حمض اليوريك أعلى بكثير في المرضى الذين يزنون أقل من 2,5 كلغ وبفارق معنوي (t-test = 2.688، P = 0.008). علاوة على ذلك ، كان تركيز حمض اليوريك لدى مرضى الخدج أعلى بفارق معنوي مقارنة بغير الخدج (t-test = 2.435 ، P = 0.017). كما وجدت النتائج أن المرضى الذين يحتاجون إلى تدخل طبي لديهم تراكيز أعلى من حمض اليوريك t-test = 2.395) ، P = 0.024). بالإضافة إلى ذلك ، كان لدى المرضى الذين توفو تراكيز حمض اليوريك أعلى بشكل معنويمقارنة بالذين بقوا احياء t-test = 8.545) ، P = 0.001). بينما أظهرت العوامل ألاخرى بما في ذلك الجنس والعمر تأثير غير معنوي. اما عيارية بروتين سي التفاعلي عالي الحساسية كانت اعلى بشكل معنوي لدى المرضى الخدج من المجموعة الضابطة (t-test = 2.066، P = 0.042). ومع ذلك ، فشلت العوامل ألاخرى بما في ذلك الجنس والعمر والوزن والتدخل المطلوب ومصير الطفل للوصول إلى مستويات ذات دلالة إحصائية.الاســـــــــــــــتنتاج: خلصت الدراسة إلى أن تركيز حمض اليوريك في المصل وعيارية بروتين سي التفاعلي عالي الحساسية يمكن استخدامه كمساعدات تشخيصية لمتلازمة الضائقة التنفسية الحادة عند حديثي الولادة ، وقد اتوافقت بعض خصائص المريض والأم بشكل معنوي بهذه النتيجة.الكلمات المفتاحية: متلازمة الضائقة التنفسية الحادة ، حمض اليوريك ، بروتين سي التفاعلي عالي الحساسية

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