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Article
Pre-Treatment Of Erythrocytes With Garlic Or Tea Tree Oil Promotes Oxidation Of The Peroxiredoxin 2 Protein And Makes The Cells Less Susceptible To Infection By Plasmodium Falciparum

Authors: KATHRYN A. SCHULLER --- IAN MENZ --- ROSHNI THATTENGAT --- 3SARMAD A. M. AL-ASADI
Journal: Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) ISSN: 18172695 Year: 2019 Volume: 45 Issue: 1A Pages: 45-56
Publisher: Basrah University جامعة البصرة

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Abstract

Plasmodium falciparum, the causal organism of the most deadly form of human malaria, lacks catalase and glutathione peroxidase enzymes and thus is highly dependent on peroxiredoxin (Prx) enzymes for its defence against oxidative stress. In addition to its own five Prx enzymes, P. falciparum also uses the human Prx2 protein which it imports from the host erythrocyte. Here we have investigated the effects of pre-treatment of uninfected erythrocytes with increasing concentrations of garlic or tea tree oil on the redox/oligomerization state of the Prx2 protein and on the P. falciparum parasitemia in the erythrocytes. Both oils were shown to be able to disrupt the Prx2 redox state in pre-treated uninfected erythrocytes by promoting oxidised dimer formation. Garlic oil was a more potent promotor of the oxidation/inactivation of the Prx2 protein than tea tree oil. The results also showed that both oils promoted oxidation/inactivation of the Prx2 protein at the 2nd generation ring stage and also appeared to promote oxidation of other sulfhydryl group-containing proteins. Additionally, pre-treatment of uninfected erythrocytes with the test oils made the erythrocytes less susceptible to infection by P. falciparum at the 2nd generation ring stage (new infections). Garlic oil was more effective than tea tree oil in this respect. Thus, oxidation of the Prx2 protein might be involved in decreasing the susceptibility of pre-treated uninfected erythrocytes to infection by P. falciparum. These results suggest that garlic and tea tree oils could be used as antimalarial drugs


Article
The Effect of Heat Stress on Oxidative Stress and Antioxidant Status in Local Quail Hens Supplemented with Onion and Garlic Oils
تأثير الإجهاد الحراري للإجهاد التأكسدي والحالة التأكسدية بإضافة زيتي البصل أو الثوم لعليقة إناث السمان المحلي

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Abstract

This study was conducted to investigate the potential of onion and garlic oils and their mixture supplementation under heat stress on the egg production, egg weight, total lipid concentration and antioxidant activity of local quail hens. Sixty birds at 42 weeks' age were evenly divided randomly into 4 treatments: feed in control (T0) free of onion and garlic oil, T1 and T2 were supplemented 0.2% in each onion or garlic oil, T3 was supplemented mixture of (0.1% onion + 0.1% garlic oil) respectively. The birds reared in temperature-controlled room (35-38°C) at afternoon. The results of enzymatically analysis of catalase, superoxide dismutase, Glutathion-Px activity concentrations, egg production percentage, egg weight and egg mass had significantly higher (P≤0.05) in the most treatments of onion and garlic oils and their mixture supplemented diet. While, significantly lower (P≤0.05) in total cholesterol, hydrogen peroxide (H2O2) and Malondialdehyde (MDA) in onion and garlic oils and their mixture supplemented diet compared with control of blood serum, liver tissue and egg yolk of quail hens under heat stress.

أجريت هذه الدراسة لبحث أمكانية إضافة زيتي البصل والثوم وخليطهما تحت ظروف الإجهاد الحراري في التركيز الكلي للكولسترول ونشاط انزيمات الأكسدة في الكبد، مصل الدم وصفار البيض، كذلك على انتاج البيض، وزن وكتلة بيض إناث السمان المحلي. وزعت 60 انثى السمان وبعمر 42 اسبوعا عشوائيا على أربع معاملات: T0 معاملة السيطرة ( بدون أية إضافة لزيتي البصل والثوم)، T1 و T2 أضيفت اليهما 0.2% زبت البصل و زيت الثوم على التوالي والمعاملة T3 أضيفت إليها 0.1% مزيج لكل من زيتي البصل والثوم. ربيت الطيور في غرفة بدرجة حرارة (35-38) °م. نتائج التحليل لنشاط أنزيمات CAT، SOD و GSH-Px وانتاج البيض ووزنه ارتفعوا معنويا (P≤0.05) في معظم معاملات إضافة زيتي البصل والثوم وخليطهما على معاملة السيطرة. في حين تركيز الكولسترول الكلي ونشاط انزيمي H2O2 و MDA انخفضوا معنويا (P≤0.05) في معظم معاملات إضافة زيتي البصل والثوم وخليطهما على معاملة السيطرة في الكبد، مصل الدم وصفار البيض في اناث السمان تحت ظروف الأجهاد الحراري. لوحظ تفوق معاملة اضافة زيت الثوم على البصل في معظم الصفات.

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