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Article
A comparison of the prevalence of anti-Liver/Kidney Microsome antibody type-1(LKM-1) in individuals with chronic hepatitis C and those with autoimmune hepatitis

Author: Eman Sh. AL- Obeidy* M.Sc د. ايمان شاكر العبيدي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 2 Pages: 245-248
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: Autoantibodies are frequently found in patients with chronic hepatitis C, which suggests that HCV elicit an immune response in the host. ). The relationship of type-2 AIH to chronic hepatitis C (HCV) is an interesting and as yet unresolved problem. Importantly, antibodies to liver/kidney microsome type-1 (LKM1), the serologic marker of type 2-AIH, have been recognized in serum of some patients with chronic hepatitis C . Methods: Anti-liver kidney microsome type 1 (LKM-1) autoantibodies were studied by indirect immuno florescence assay (IIF) and confirmed by immunoblot in the serum of 73 Iraqi patients with autoimmune hepatitis (AIH) in comparison with 50 patients control (HCV infection) and 50 healthy individuals.Results: anti-LKM 1 with high titers (1/160) present in serum of all patients with AIH-2, whereas they present in titer (≤1/80) in serum of 19 patients with chronic HCV, while all healthy control group are negative.Conclusion: AIH is easily distinguished from chronic viral hepatitis since patients with AIH are more commonly women than those with chronic viral hepatitis, and they have higher serum levels of aspartate aminotransferase, bilirubin, gamma-globulin, IgG, alkaline phosphates, and higher frequency of multilobular necrosis on histologic examination than counters with chronic viral hepatitis. Keywords: HCV, AIH, , IgG

Keywords

: HCV --- AIH --- IgG


Article
Serum Immunoglobulins Levels in Autoimmune Hepatitis of Iraqi Patients

Author: Eman Sh. AL- Obeidy* BSc, PhD د. ايمان شاكر العبيدي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 4 Pages: 396-398
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: Autoimmune hepatitis (AIH) is an unresolving inflammation of the liver of unknown cause associated with interface hepatitis on histological examination,hypergammaglobulinemia and presence of circulating autoantibodies. Antibody-mediated tissue injury might be responsible for tissue injury in AIH therefore; a number of studies have been focused on the immunoglobulines in these patients .The aim of the study is to estimate the level of immunoglobulins (IgG, IgA and IgM ) in different types of Autoimmune hepatitis ( AIH).Patients and Methods: The study was performed on 73 Iraqi patients with autoimmune hepatitis (AIH), attending the teaching hospital for gastroenterology and liver disease in a period between November 2003 and July 2004. : Anti- soluble liver ANA, SMA, LKM-1, and LC-1 Abs were detected using Enzyme-Linked Immunosorbent Assay (ELISA) technique. Anti-SLA/LP was detected using the Euroline method.Quantitation of serum IgG, IgA and IgM of the study groups were carried by single radial immudiffusion (SRID) test. Equal volumeResults: Significant differences was observed between AIH patients and healthy control group in the mean level of IgG ,IgA and IgM. In addition, level of IgA in the sera of patients with type 2 was lower than those of type 1 and 3.Conclusion: It was conclude that in patients with AIH, the Immunoglobulin G was significantly higher in type-1, whereas IgA was significantly lower in AIH-2 patients compared to other types of the disease.Keywords: Autoimmune hepatitis, Immunoglobulin G.


Article
C3 and C4 complement components in autoimmune hepatitis.

Author: Eman Sh. AL-Obeidy M.Sc. د. ايمان شاكر العبيدي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 3 Pages: 331-333
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: . Autoimmune hepatitis (AIH) is an unresolving inflammation of the liver of unknown cause associated with interface hepatitis on histological examination,hypergammaglobulinemia and presence of circulating autoantibodies. Antibody-mediated tissue injury might be responsible for tissue injury in AIH therefore; a number of studies have been focused on the complement system in these patients .The aim of the study is to estimate the level of complement (C3 and C4) in different types of Autoimmune hepatitis (AIH).Methods: The study M’as performed on 73 Iraqi patients with autoimmune hepatitis (AIH), attending the teaching hospital for gastroenterology and liver disease in a period betM'een November 2003 and July 2004. : Anti- soluble liver ANA , SAM,LKA1-1 ,and LC-1 Abs were detected using Enzyme-Linked Immunosorbent Assay (ELISA) technique . Anti-SLA/LP was detected using the Euroline method.Quantitation of serum C3 and C4 of the study groups were carried by single radial immudiffusion (SRID) test. Equal volumeResults: Significant differences M’as observed between AIH patients and healthy control group in the mean level of C3 and C4. In addition, level of C4 in the sera of patients with type 2 was lower than those of type 1 and 3.Conclusion:It M’as conclude that patients with MH do not have abnormal catabolism of complement or increased level of cleavage products of C3, whereas, the depression of complement level may reflect decreased hepatic synthesis.

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Article
Possible association of HLA class-I Molecules with autoimmune Hepatitis in Iraqi patients

Authors: Raghad J H AL-Akayshi --- Khalida M. Mousawy --- Eman Sh. AL-Obeidy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 1 Pages: 43-47
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: genetic factors were considered to play a possible role the development of autoimmune hepatitis.
Patients and methods: polymerase chain reaction-sequence specific primers (PCRSSP) was the method used to asses HLA-typing of 100 blood samples of 60 AIH patients and 40 healthy normal controls.
Results: comparison between AIH patients and healthy controls showed several antigens deviations in their frequencies. HLA-A*113 (A1/-/Null) observed to play a possible risk factor in this disease while significant loss of HLA-A*2 allele were clearly observed which prompt us to believe that it could act as a protective factor, on the other hand, increased frequency of HLA-B*8 & B*14 were statistically significant in AIH which is most likely to be considered as a rather risk factor, while most HLA-B*16 were lost which led us to think of being acting as a rather protective factor.
Conclusions: this finding reflects a preliminary picture that HLA antigens might play a role in AIH susceptibility and further studies are worth being carried out.
Key words: AIH, HLA antigen, PCR-SSP.

Keywords


Article
Possible Association of HLA-DR and DQ Molecules with Autoimmune Hepatitis in Iraqi Patients

Authors: Raghad J H AL-Akayshi --- MBChB, PhD --- Eman Sh. AL-Obeidy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 1 Pages: 49-52
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The genes of this region seem to influence susceptibility to certain diseases.
Patients and methods: Polymerase chain reaction-Sequence Specific Primers PCR-SSP is the method used to asses HLA-typing of 100 blood samples of 60 AIH patients and 40 healthy normal controls.
Results: An increased frequency of HLA-DR3, DR4 and DR7 was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively, while DR*0211 (DR2) may be formed the basis for protection against the disease. HLA-DQ on the other hand, yielded on association in Iraqi patients with AIH.
Conclusions: This finding demonstrated that HLA-DR3, DR4 and DR7 might play a role in AIH susceptibility.
Key words: autoimmune hepatitis, Human leukocyte antigen (HLA), PCR –SSP assay.

References:

Keywords


Article
Viruses as a Trigger for autoimmune Hepatitis in susceptible Individual

Authors: Bassim A. Asker --- Aswad H. Al.Obeidy --- Eman Sh. AL- Obeidy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2008 Volume: 50 Issue: 4 Pages: 471-474
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Autoimmune hepatitis (AIH) is a rare chronic liver disease of known etiology, characterized by hypergammaglobulinemia, characteristic auto antibodies, and a favorable response to immunosuppressive treatment.Strong circumstantial evidences denoted that there is quite long list of environmental factors such as (food additives and drugs), viruses and toxins which play an important role in precipitating this disease.
Patients and Methods: the study was performed on 13 Iraqi patients with acute viral hepatitis, attending the Teaching Hospital for Gastroenterology and Liver Disease and Al-Khadymia Teaching Hospital in the period between November 2003 and July 2005. ANA, SMA and anti-LKM 1 were detected by immunoflurescent technique whereas SLA/LP Ab was detected by Euroline method.
Results: the thirteen patients with acute viral hepatitis were developing AIH after 3 months.Conclusion: acute viral hepatitis is a trigger of AIH.

Keywords

Viral hepatitis --- AIH


Article
Correlation between some immunological parameters and clinicalpresentation in RA patients

Authors: Samira N. AL- Naim --- Eman Sh. AL- Obeidy --- Nahida R. Abbas*
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2008 Volume: 50 Issue: 2 Pages: 235-240
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Complement (C) & CRP in patients with Rheumatoid arthritis
(RA)'could be trigger disease activity.
Aim: To study the correlation between C, CRP, IgM,, IgG, IgA & some clinical
presentation in RA patients.
Methods: Latex agglutination test(AG) & single radial immunoassay(SRIA) were
used to asses CRP, IgMJgGJgA,, C, in 74 patients with RA.
Results: IgA & IgG were significantly increased, while the mean ofC3, C4 were
slightly elevated in RA patients .
Conclusion: There is correlation between IgG and IgM with joint deformity and joint
swelling respectively, while C3 was showed statistically significant P<0.01 with joint
stiffness, joint swelling and Rheumatoid nodule, where as CRP was statistically
significant P<0.01withjoint swelling.. Key words : RA.C.CRP, IgM, IgG, IgA, SRI A,
AG.

Introduction:

Rheumatoid arthritis (RA) is one of the
mysterious autoimmune diseases which is still
unresolved characterized by inflammation of
synovial membrane, principally affecting
peripheral joints in a symmetric fashion,
commonly leads to cartilage destruction, bone
erosion and joint deformities; extra-articular
manifestations such as vasculitis and
subcutaneous nodules can also occur, hence
it's course is quite variable (1 -3).

Keywords


Article
Anti-Liver membrane Antibody predict outcome of Autoimmune Hepatitis

Authors: Akram A. Najeeb --- Batool A. Al-Haidary --- Eman Sh. AL- Obeidy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2008 Volume: 50 Issue: 3 Pages: 395-399
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Autoantibodies against liver membrane are the specific
autoantibodies found in AIH. Cytochrome P450 1A2 has been identified as the
target autoantigen for these autoantibodies
The aim of the study : is to evaluate the prevalence and clinical relevance of
LMA in AIH
Methods: The study was performed on 73 Iraqi patients with chronic active hepatitis
(CAH) of unknown cause , attending the teaching hospital for gastroenterology and
liver disease in a period between November 2003 and July 2004. Anti-liver membrane
antibodies were studied by IFA technique.
Results: LMA was detected in the sera of 28(57.14)and 3(37.5%) patients with type 1
and type 3 AIH respectively, but never in the sera of healthy group .
Conclusion: It was concluded that LMA is present in type-1 and type-3 AIH but not in
sera of patients with type-2 or in healthy control group.

Keywords


Article
The Vallue off Pollymerase Chaiin Reacttiion iin tthe Diiagnosiis off Tubercullous Meniingiittiis iin a Samplle off Iraqii Pattiient

Authors: Akram M. Al-Mahdawi --- Kareem M.Al-Tameemi --- Eman Sh. Al-Obeidy --- ***,Laith Ahmed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 3 Pages: 382-391
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Tuberculous meningitis (TBM) is a medical emergency. Early diagnosis is of utmost importance to minimize morbidity and mortality. Polymerase chain reaction (PCR) seems to be a promising test for rapid and early diagnosis of TBM.OBJECTIVE:To investigate whether PCR detects tubercle bacilli in CSF specimens that are missed by direct microscopy and culture, and if so whether PCR has significant diagnostic value compared to conventional methods.METHODS:PCR, culture and acid- fast bacilli (AFB) were performed on CSF samples taken from 43 patients with TBM (based on clinical features and cytochemical parameters of the CSF) and 15 with non- TBM as control group.RESULTS:Of the 43 CSF specimens from highly probable TBM patients, 33 were positive by PCR (76.7%), whereas only 5 was acid-fast microscopy (AFM) positive (11.6%) and 22 were culture positive (55.2%). No positive results were found by AFM, culture or PCR in the non-tuberculous control group.CONCLUSION:The results of this study indicate that application of PCR is extremely useful for the diagnosis of TBM.The PCR is superior to the currently available techniques for the diagnosis of tuberculous meningitis in terms of sensitivity, specificity and rapidity and can play a critical role in the diagnosis of suspected cases.


Article
Production of Different Cytokines in Acute and Chronic Hepatitis C Virus.
انتاج مختلف السايتوكينات في التهاب الكبد الفايروسي ج الحاد والمزمن

Authors: Khalid A. Al-Khazraji د.خالد الخزرجي --- Eman Sh. Al- Obeidy د.ايمان العبيدي
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2010 Volume: 23 Issue: 2 Pages: 134-140
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Summary:Background: the role of pro-inflammatory cytokines has been documented in acute phase reactions in the liver, in normal proliferation of hepatocytes, in autoactivation of Kupffer cells and proliferation of immunocyte, in cirrhotic processes in the liver and in regeneration of the organ in chronic hepatitis C.Objective: assess whether there is a differential production of Th1 or Th2 associated cytokines in the course of acute and chronic HCV infection.Patients & methods: Serum level of IL2, INF gamma (Th1 associated cytokines) and IL10 (Th2 associated cytokines) were measured in 12 patients with acute HCV, 50 patients with chronic HCV infection and 50 healthy volunteers. Results: mean serum level of IL-2 was significantly higher in chronic hepatitis C (30.5 ± 16.02) than in acute cases (26.9 ± 13.01) and controls (11.3 ± 2.03). Acute HCV cases had a higher mean IL-10 level (99.3 ± 17.0) than controls (5.0 ± 2.0) and chronic HCV cases had a higher mean IL-10 level (100.9 ± 53.7) than acute HCV cases. Mean serum level of INF gamma where nearly equal in all three groups. Conclusion: this study suggests weak response of Th1 cells in acute HCV infection and a possible suppressive Th2 cell response in chronic HCV, which might explain the failure of viral clearance and a weak cellular immune response.Key wards: Chronic hepatitis-C; Pro-inflammatory cytokines; IL-2; IL-10 NF-gamma.

الملخص:الخلفية:تم اثبات دور السايتوكينات المؤيدة للالتهاب في تفاعلات المرحلة الحادة في الكبد, في التكاثر الطبيعي لخلايا الكبد,في التنشيط الذاتي لخلايا كفر,في تكاثر الخلايا المناعية,في عمليات التشمع في الكبد,وفي تجدد العضو في التهاب الكبد الفايروسي ج المزمن. الاهداف:تقييم فيما اذا كان هناك انتاج نوع مختلف من السايتوكينات المرتبطة بخلاياTالمساعدة نوع1و2(Th1andTh2)خلال مرحلة العدوى بالتهاب الكبد الفايروسي ج الحاد والمزمن.المرضى وطرق العمل:تم قياس مستوى المصل من انترلوكين2 و انترفيرون كاما(سايتوكينات مرتبطة بخلاياTالمساعدة نوع1Th2)في 12 مريضا مصابا بالتهاب الكبد الفايروسي ج الحاد و50 مريضا مصابا بالتهاب الكبد الفايروسي ج المزمن و50شخصا اصحاء متطوعين.٭٭ معدل مستوى المصل من انترلوكين2كان اعلى بصورة ملفتة للنظر في التهاب الكبد الفايروسي ج المزمن(16,02±30,5)من التهاب الكبد الفايروسي ج الحاد(26,9±13,01)والكونترول(11,3±2,03) وسجلت حالات التهاب الكبد الفايروسي ج الحاد مستويات اعلى من انترلوكين10(99و3±17)من الكونترول(5±2).اما حالات التهاب الكبد الفايروسي ج المزمن فسجلت مستويات اعلى من انترلوكين10(100,9±53,7)من التهاب الكبد الفايروسي ج الحاد.٭٭معدل مستوى المصل من انترفيرون كاما كان تقريبا متساويا في الحالات الثلاث.الاستنتاجات:هذه الدراسة تبين استجابة ضعيفة للخلايا المساعدة1Th1في الحالات الحادة واستجابة مثبطة محتملة للخلايا المساعدة2Th2في الحالات المزمنة والذي من الممكن ان يفسر فشل تنقية الفايروس وضعف الاستجابة المناعية الخلويةالكلمات المفاتيح: التهاب الكبد الفايروسي ج المزمن,السايتوكينات المؤيدة لللالتهاب,انترلوكين1,انترلوكين10,انترفيرون كاما

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