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Article
Etanercept ameliorate cardiac damage and Apoptosis Induced by Myocardial Ischemia/Reperfusion in male mice.

Authors: Najah R. Hadi --- Fadhil G. Yousif --- Maitham G Yousif --- Saif M. Hassan
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 105-118
Publisher: University of Kufa جامعة الكوفة

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Abstract

Ischemia-reperfusion of cardiac tissues may lead to a prominent damage of the cardiomyocyte through either necrosis or apoptosis that seems to be the predominant modes of death during this period. In this study, we investigated the effects of Etanercept in regional ischemia/ reperfusion injury and apoptosis. White albino adult male mice were divided into four groups (six mice per group). Sham group, mice were subjected for all surgical procedure without ligation of the left interior descending coronary artery (LAD). Control group, in which LAD was ligated. Control vehicle and Etanercept treated groups, mice subjected to the surgical procedure with ligation of LAD for 30 minutes followed by 120 minutes reperfusion, mice treated with normal saline (etanercept vehicle) and Etanercept (5 mg/kg, I.P. 5 minute before reperfusion). At the end of reperfusion, cardiac tissue caspase 3 and Bcl-2, as well as plasma cardiac troponin I (cTnI) were measured. It has been found that Etanercept treated group showed significant reduction (P˂0.01) in caspase 3and cTnI but increase (P˂0.01) the level of the Bcl-2 as compare with the control groups. Histopathology study revealed that the treatment with Etanercept significantly (P˂0.01) improved cardiac injury as compared with control groups and the total severity scores showed showed 16.7 % of the group had no damage and 50% had mild cardiac injury and 33.3% had moderate cardiac injury of Etanercept treated group. It is concluded that Etanercept reduces cardiac damage and apoptosis associated with ischemia/reperfusion injury.


Article
The Relationship between Human Papilloma virus (HPV) infection and Gene Expression of miRNA-744, BCL-2, CASPASE-3 genes Associated with Cervical Cancer in Iraqi Patients

Author: Tabark S. Jassim , Abdul Hussein M. AlFaisal
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 30-35
Publisher: Baghdad University جامعة بغداد

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Abstract

: Most types of HPV are harmless and some types are high risk and can cause cervical cancer or abnormal cells in the lining of the cervix that sometimes turn into cancer. A total number of 90 subjects (70 Iraqi patients with cervical dysplasia (abnormalities) and 20 apparently healthy women) were included in this study. The patients and healthy were aged between 25-55 years. The molecular detection in our study showed that 30 of patients were positive to HPV and 40 patients were negative to virus compared to negative results in healthy women. The most common genotypes is HPV-16. Also multiple infections of HPV-HR were observed in the infected women. The correlation between gene expression, histopathology and HPV virus showed a significant increase of folding values of the genes mi-RNA744 and caspase-3 parallel to decrease of folding value of the gene Bcl-2 which indicated that the apoptosis rate was increased with the histopathology grade where the highest level of expression of the genes was in carcinoma. The results also revealed that mi-RNA744 could be up regulator of the gene caspase-3 and down regulator of the gene Bcl-2.


Article
IMMUNOCYTOCHEMICAL DETECTION OF SOME APOPTOSIS REGULATING PROTEINS (P53 AND BCL-2) IN PERIPHERAL BLOOD LYMPHOCYTES OF RHEUMATOID ARTHRITIS PATIENTS
كشف مناعي خلوي كيميائي لبعض البروتينات المسيطرة على عملية الذوي (P53 و Bcl-2) في خلايا الدم اللمفيه المحيطيه لمرضى التهاب المفاصل الرثوي

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Abstract

Background: There are several regulatory proteins involved in the control of lymphocyte apoptosis. Their impairment may play a role in the pathogenesis of several autoimmune diseases. Recent studies have reported impairment in the apoptosis of peripheral blood lymphocytes (PBLs) in patients with rheumatoid arthritis (RA). Objective: The aim of this study is to evaluate the cellular expression of P53 and BcI-2 proteins in the PBLs and their roles in the apoptotic process, and correlate their cellular expressions with the percent of peripheral T cell population. Methods: This study involved forty-six RA patients were examined and compared with 17 healthy control individuals of similar ages. Lymphocytes were separated from peripheral blood samples, the assessment of their cellular expression of CD3 and regulatory proteins p53 and Bcl-2 by immunocytochemistry staining method. Results: The results showed abundant accumulation of CD3 T lymphocytes in the peripheral circulation of RA patients in comparison with controls. A highly significant increased percentage of Bcl-2 protein expression in RA PBLs, compared to healthy control (p<0.001) while there was no such statistical difference regarding P53 expression in PBLs from both groups (p=0.278). The results of linear regression showed a significant correlation between the increased peripheral blood T lymphocytes and cellular percentage of Bcl-2 protein expression (p<0.001), while there was no such correlation with the percentage of P53 expression (p=0.587).Conclusion: in conclusion of these results, we found an increase in the peripheral blood T lymphocytes from patients with RA that could be resulted from the noticed up-regulation of cellular expression of Bcl-2 protein, rather than with changes in cellular expression of P53 protein.

خلفية الدراسة: هنالك العديد من بروتينات السيطرة التي تشترك في عملية ذوى الخلايا اللمفيه وان الخلل الحاصل فيها قد يلعب دوراً مهماً في أمراضية العديد من الأمراض ذاتية المناعه. لقد ذكرت الدراسات الحديثة وجود خلل في عملية الذوي في خلايا الدم اللمفيه ألمحيطيه لمرضى التهاب المفاصل الرثوي.هدف الدراسة: إن الهدف من هذه الدراسة هو قياس نسبة الإظهار الخلوي لبروتين P53 و Bcl-2 في خلايا الدم اللمفيه المحيطية لمرضى التهاب المفاصل الرثوي ودورها في عملية الذوى, كذلك معرفة العلا قه بين نسبة الإظهار الخلوي لهما مع نسبة خلايا الدم اللمفيه التائية المحيطية.طريقة العمل: شملت هذه الدراسة ستة وأربعين مريض مصاب بالتهاب المفاصل الرثوي وقورنوا ﺒ17 شخص سليم ذوي أعمار متقاربة. حيث فصلت خلايا الدم اللمفيه من عينات الدم المحيطي وتم فحص نسبة الإظهار المناعي الخلوي للمعلم المناعي CD3 و بروتينات السيطرة P53 و Bcl-2 فيها بطريقة التصبيغ المناعي الخلوي.النتائــج: لقد أظهرت النتائج تجمع خلايا الدم اللمفيه التائية المحيطية في مجرى الدم المحيطي لمرضى التهاب المفاصل الرثوي مقارنة بمجموعة السيطرة. وجد فرقاً معنوياً عالياًَ في زيادة نسبة إظهار بروتين Bcl-2 في خلايا الدم اللمفيه المحيطية لمرضى التهاب المفاصل الرثوي مقارنة بمجموعة السيطرة (P<0.001) بينما لا يوجد فرق معنوي بالنسبة لنسبة الإظهار الخلوي لبروتين P53 بين المجموعتين .(P=0.278) أظهرت نتائج الانحدار الخطي وجود علاقة معنوية بين زيادة الخلايا اللمفيه التائية ونسبة إظهار بروتين Bcl-2 (P<0.001) بينما لا توجد تلك العلاقة مع نسبة إظهار بروتين P53 (P=0.587).الاستنتاجات: تم الاستنتاج من خلال نتائج هذه الدراسة وجود زيادة ملحوظة في عدد الخلايا اللمفيه التائية المحيطية للمرضى المصابين بالتهاب المفاصل الرثوي والذي قد يكون ناتج عن خلل في عملية الذوي المتمثلة بالزيادة الملحوظة في الإظهار الخلوي لبروتين Bcl-2 بغض النظر عن نسبة الإظهار الخلوي ﻟP53 بروتين.


Article
Apoptotic Activity of methionine γ- lyase on several cancer cell lines
فعاليه الموت المبرمج للأنزيم methionine γ-lyase في بعض خطوط الخلايا السرطانية

Authors: Nisreen H. Odaa نسرين iهادي عودة --- Nahi Y. Yaseen ناهي يوسف ياسين --- May T. Flaih مي طالب فليح
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2013 Volume: 6 Issue: 1 Pages: 33-37
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

The apoptotic activity of methionine γ- lyase from Pseudomonas putida on cancer cell lines was indicated by measuring the concentration of cytochrome c in the supernatants of cell lines. The result revealed high concentration of cytochrome c in the supernatants of cancer cell lines (RD, AMGM and AMN3) respectively while the concentration of anti-apoptotic protein (Bcl-2) was very low.

درست فعالية الموت المبرمج للأنزيم methionine γ-lyase المنتج من بكتريا Pseudomonas putida بواسطة قياس تركيز سايتوكروم سي في رائق الخلايا السرطانية , آذ أظهرت النتائج تراكيز عالية للسايتوكروم سي في رائق الخلايا السرطانية RD,AMGM and AMN3 بالتتابع بينما كانت تراكيز البروتين المثبط للموت المبرمج(Bcl-2) قليله جدا.


Article
The Role of Helicobacter pylori CagA infection on the presence of Bcl-2 marker in gastric carcinoma by immune-histochemical analysis and in situ hybridization technique
Bcl-2في الخلايا السرطانية في المعدة وارتباطه مع المعلمات السريرية المرضية و تحديد تأثير العدوى ببكتيريا ألملويَات البوابيَة على امكانية ظهور ألخلية Bcl-2 في حالات سرطان المعدة الغدي

Authors: Salma L Dahash سلمى لعيبي دهش --- Isra'a M Al-Sudani اسراء السوداني --- Wasan A Bakir وسن أ باقر
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2016 Volume: 29 Issue: 2 Pages: 20-14
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Bcl-2 plays a major role in the process of apoptosis and their dysfunction underlies carcinogenesis.Objectives: The study objective was to assess the expression of Bcl-2 in gastric cancer cells in correlation with clinic-pathological parameters and to determine the effect of H. Pylori infection on the expressions of Bcl2 in gastric adenocarcinoma.Methods: Using immune-histochemical analysis and in situ hybridization technique were used to measure Bcl-2 protein and CagA H. pylori gene expressions respectively and its relation to clinic-pathological parameters were observed.Results: The results revealed that a significant differentiation between Bcl-2 expression on the patients with gastric cancer and control group. Also we found thatBcl-2 expression significantly higher in intestinal type in comparison to that diffuse type (p<0.001). But no significant association between positive Bcl-2 expression and the presence of CagA-H. pylori in patients with gastric carcinoma (p>0.05).Conclusion: Bcl-2 expression in gastric cancer patients was suggested of its association with gastric carcinoma especially intestinal type. Thus, detection of Bcl-2 markers might be a useful early diagnostic in gastric cancer. H pylori enhance Bcl-2 protein levels which cause deregulation of these apoptosis-associated genes that may play a role in the development of gastric adenocarcinoma

المقدمه: يلعب Bcl-2 دورا رئيسيا في عملية موت الخلايا المبرمج والاختلال الوظيفي الذي يكمن من وراءه التسرطنالهدف الدراسة: تقييم تعبيرBcl-2في الخلايا السرطانية في المعدة وارتباطه مع المعلمات السريرية المرضية و تحديد تأثير العدوى ببكتيريا ألملويَات البوابيَة على امكانية ظهور ألخلية Bcl-2 في حالات سرطان المعدة الغدي.المواد وطرق العمل: باستخدام التحليل المناعي الكيميائي وتقنية التهجين الموضعي لتقييم تعبير Bcl-2 وببكتيريا ألملويَة البوابيَة الحاملة لجين Cag A على التوالي وعلاقتهم مع المعلمات السريرية المرضية.النتائج: أظهرت نتائج هذه الدراسة ان هناك اختلاف معنوي بين التعبير المناعي الكيميائيBcl-2 لمرضى سرطان المعدة ومجموعة السيطرة(p<0.001). ووجد ايضا ان هناك فرق معنوي عالي للتعبير المناعي الكيميائي Bcl-2 بين التوع المعوي بالمقارنة مع النوع المنتشر(p<0.001). لم تجد الدراسة الحالية اي علاقة معتدة احصائيا بين ظهور التعبير المناعي الكيميائي Bcl-2و ظهور بكتريا الملويات البوابية الحاملةلجين (Cag A) في مرضى سرطان المعدة(p>0.05).الاستنتاج: ظهور Bcl-2مرضى سرطان المعدةتوحي بارتباطها مع مرض سرطان المعدة وخصوصا النوع المعوي وعليه فأنه َربما يفيد في الكشف المبكر عن سرطان المعدة. بكتيريا ألملويَات البوابيَة قد تعزز ارتفاع مستويات Bcl-2 الذي يسبب تحرير هذه الجينات المرتبطة بموت الخلايا المبرمج والتي قد تلعب دورا في تطوير سرطان المعدة


Article
Immunohistochemical evaluation of BCL-2 and KI-67 proteins in colorectal adenoma
تقييم التعبير المناعي الكيميائي النسيجي للمعلمات الورمية(KI67, BCL2)

Authors: Khitam R. Al-Khafaji ختام رزاق الخفاجي --- Areege N. Abd اريج نعمة عبد
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2014 Volume: 56 Issue: 2 Pages: 191-194
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Development of colorectal adenocarcinoma principally occurs via the adenoma- carcinoma sequence of a multiple step process of tumor progression. This results from an accumulation of genetic changes in cells of intestinal mucosa. The use of molecular biomarkers may further aid in determining the risk of changing to carcinoma.Objectives: To evaluate the expression of Bcl-2 and Ki-67 in colorectal adenomas and correlate the observed levels with age, gender, histological type, grades of dysplasia as well as to the levels of these two markers with each other.Materials and Methods: A retrospective stu dy of fifty colorectal biopsies were selected randomly, 40 cases were with different grades of colorectal adenomas and 10 cases with normal mucosa as a control group; covering the period from 2010 till 2012. All the cases collected from the Gastroenterology and Hepatology Teaching Hospital. The biopsies were re-examined histologically and then stained immunohistochemically for Ki-67 and Bcl-2.Results: There was a significant correlation only between Ki-67 expression and grade of adenoma and there was a significant correlation between Ki-67 and Bcl-2 expression in adenoma. Also there was a significant correlation between age of the patients and histologic type.Conclusions: The assay of Ki-67 and Bcl-2 is valuable in detecting the risk of early colorectal carcinogenesis in patients with colorectal adenomas. Ki-67 expression increases with progression of the grade of adenoma while BCL-2 decrease with progression of the grade.Key words: Colorectal adenoma, Carcinomatous changes, KI-67, BCL-2, Immunohistochemical stain.

الخلاصة:السرطان‘عملية متعددة الخطوات في تحول الورم تنتج من مجموعة تغييرات جينية في خلايا الغشاء المخاطي للأمعاء. إستخدام المؤشرات الحيوية الجزيئية ممكن ان يساعد أكثر في تحديد الخطورة للتحول الى السرطان.هدف الدراسة: الهدف الرئيسي من هذه الدراسة هو تقييم التعبير المناعي الكيميائي النسيجي للمؤشرات الجزيئيةBCL-2, KI-67 في الاورام الحميدة في القولون والمستقيم ومقارنة المستويات الملاحظة مع العمر،الجنس،النوع النسيجي و درجات النمو الشاذ ثم مقارنة مستويات كلا المؤشرين مع بعضهما.المواد وطرق العمل: تم جمع خمسين عينة من القولون والمستقيم عشوائياً. اربعون عينة من مرضى مصابين بمختلف درجات النمو الشاذ وعشر عيينات من نسيج طبيعي للقولون والمستقيم. تم جمع العينات من مستشفى الجهاز الهضمي في مدينة الطب وكانت من عام 2010 ولغاية 2012. تم جمع المعلومات من ملفات المرضى وتقارير الفحص النسيجي. قطعت البلوكات الشمعية وتم فحصها بعد صبغها بصبغة الهيماتوكسلين و الأيوسين.ثم استخدمت الصبغات المناعية النتائج:اثبتت النتائج وجود علاقة وثيقة بين درجة النمو الشاذ و شدة تركيز هذه المؤشرات الجزيئية و ايضاً ان )هناك علاقة بين العاملين BCL-2 وki-67).الأستنتاج: من الدراسة تبين وجود علاقة ايجابية بين هذه المؤشرات الجزيئية ودرجات النمو الشاذ للأورام الحميدة في القولون والمستقيم وكذلك اثبتت الدراسة وجود علاقة عكسية بين هذين المؤشرين بالنسبة لدرجة النمو الشاذ.كما تبين الدراسة وجود علاقة بين عمر المريض والنوع النسيجي.مفتاح الكلمات: ورم القولون والمستقيم الحميد , التغيرات السرطانية , المعلمات الورمية Ki-4 , Bcl-2 , الصبغات النسيجية الكيميائية


Article
Tissue Microarray Construction and Immunohistochemical evaluation of Bcl-2 Gene Expression in Iraqi and Italian Breast Cancer Samples
التقييم الكيميائي المناعي النسيجي لتعبير جين Bcl-2 في نماذج سرطان الثدي عراقية وايطالية باستخدام المصفوفة الدقيقة للنسيج

Author: Farooq Ibrahem Mohammad فاروق ابراهيم محمد
Journal: Jornal of Biotechnology Research Center مجلة مركز بحوث التقنيات الاحيائية ISSN: 18151140 Year: 2016 Volume: 10 Issue: 2 Pages: 32-38
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Breast cancer is a heterogeneous disease and remains one of the most leading causes of death among women world wild. Anti-apoptotic Bcl-2 family members inhibits cell death and promote proliferation of cancer cells. Overexpression of Bcl-2 has been shown to inhibit the initiation of apoptosis, in the presence of some stimuli including anticancer drugs in a number of systems. To study the expression of Bcl-2 tissue microarray (TMA) investigation was designed and constructed. Using 3 TMAs; the second for the 50 Iraqi breast cancer cases, one for the 30 Italian cases and the third for the 10 benign cases. Each sample represented in a triplicate in the recipient block, all these TMAs expressed to the same condition for construction and IHC staining. TMAs are a powerful, fast and economic technique. IHC was used to study the Bcl-2 expression. The results are represented as differences in the number of samples expressed the Bcl-2 and the intensity of the expression between the Iraqi and the Italian groups. Bcl-2 expression was found elevated in 26 (52%) of the 50 Iraqi breast cancer samples and 11 (36.66%) of the 30 Italian samples compared to the control samples. And in which only 1 (10%) out of 10 samples. The overexpression of Bcl-2 associated with poor prognosis for the patients. Level of Bcl-2 expression can be used as prognostic marker to monitor patient therapy. For the Iraqi cases the overexpression may be attributed to many factors like exposure to depleted uranium and bad food quality during the 90th when Iraq was under the siege of the United Nation (UN).

سرطان الثدي مرض متعدد الانواع ويبقى واحد من اكثر الاسباب المؤدية للوفاة في النساء حول العالم, اعضاء عائلة Bcl-2 المضادة للموت المبرمج تثبط موت الخلايا وتقود الى تضاعف الخلايا السرطانية. التعبير الفائض لجين Bcl-2 تم ملاحظته لمنع البداية في الموت المبرمج, في حال وجود بعض المحفزات بما فيها العقاقير المضادة للسرطان في عدة انظمة. لدراسة تعبير جين Bcl-2 تم تصميم وبناء المصفوفات الدقيقة للنسيج, تم بناء ثلاث مصفوفات الاولى تشمل 50 عينة سرطان ثدي انساء عراقيات والثانية تشمل 30 عينة سرطان ثدي لنساء ايطاليات والثالثة تحوي 10 عينات السيطرة (الحالات الحميدة). لقد تم تمثيل كل عينة بثلاث مكررات من العينة المستلمة للنماذج, لقد تم تعريض عينات المصفوفات لنفس الظروف من ناحية البناء والتصبيغ المناعي الكيميائي للنسيج, تقنية المصفوفة الدقيقة للنسيج كانت فعالة, وسريعة, واقتصادية. تم استخدام التصبيغ المناعي الكيميائي للنسيج لدراسة تعبير جين Bcl-2. النتائج اوجدت ان عائلة بروتين Bcl-2 لعبت دور تنظيمي مهم خلال القرارات الخلوية اما ان تموت او تتجاهل الموت, لقد وجد تعبير Bcl-2 مرتفعا في 26 (52%) من الخمسين عينة لسرطان الثدي العراقي, بينما للعينات الايطالية فكانت 11 (36,66%) من اصل 30 عينة ولوحظت عينة واحدة (10%) من اصل 10 عينات كانت موجبة لعينات السيطرة, التعبير الفائض ربما يعطي انطباع سيء عن المرضى او في بعض الاحيان يعطي معلم ابتدائي مرغوب به مع معلمات اخرى تستخدم في علاج المرضى. بالنسبة للعينات العراقية عالية التعبير ربما يعزى هذا الشيء الى عدة عوامل منها التعرض لليورانيوم المنضب خلال سنوات الحروب الطويلة على العراق او نوعية الغذاء السيئة خلال تسعينات القرن الماضي عندما كان العراق يقبع تحت الحصار المفروض من الامم المتحدة.


Article
The Significance of Estimation of Anti- Apoptotic Protein in Acute Leukemia Cases

Author: Waseem Fadhil Al‐Tememi
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2009 Volume: 8 Issue: 1 Pages: 53-58
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background Apoptosis is a terminal cell fate that eliminates physiological unneeded or dangerous cells. The Bcl-2 oncoprotein encodes a mitochondrial protein that blocks apoptosis. High levels of Bcl-2 protein are found in different malignancies including acute leukemias and it is thought to be directly involved in the emergence of drug resistance by disrupting &/or delaying apoptotic program and promoting tumor survival. Objectives To analyze the significance of any variation in the level of Bcl-2 expression according to disease progress (from time of diagnosis to time of remission or resistance) in cases with acute leukemia, and whether to consider this variation as a prognostic marker. Methods: Through a prospective study, 25 samples of bone marrow aspirates were taken from different patients who attended the hematology unit of Baghdad teaching hospital in the medical city from December 2005 to July 2006, who proved to have acute leukemia in its different types or subtypes(ALL and AML) & at a variable phases of the disease course. Monoclonal antibody targeted against Bcl-2 oncoprotein via immunocytochemistry staining techniques of these aspirates was performed in order to define the level of Bcl-2 protein carrying cells & looking for any differences in this expression in relation to disease progress or treatment hoping to translate these findings in terms of prognosis. Results Despite the heterogenous expression of Bcl-2 protein carrying cells in each case(0-500 cells out of 500 cells)of AML or ALL , it was found to be in positive correlation with the density of blast cell percentage in the marrow(r=0.515,p=0.008) unlike the relation with total leucocyte count (r=0.364,p=0.074). Conclusion: A good clinical clue to intractable resistant course of the disease at diagnosis is verified in this study when very high level of Bcl-2 protein carrying cells was found. This finding may have a useful clinical application through the recommendation to change therapeutic strategy, at time of diagnosis, to more aggressive protocols, which is agreed by others. There was no chance to study the application of high level of Bcl-2 protein in terms of long-term survival due to short follow up.


Article
The Significance of Estimation of Anti- Apoptotic Protein in Acute Leukemia Cases

Authors: Waseem Fadhil Al-Tememi --- Aladdin M. Al- Qasim --- Haider Sabbah
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2008 Volume: 7 Issue: 2 Pages: 53-58
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Apoptosis is a terminal cell fate that eliminates physiological unneeded or dangerous cells. The Bcl-2 oncoprotein encodes a mitochondrial protein that blocks apoptosis. High levels of Bcl-2 protein are found in different malignancies including acute leukemias and it is thought to be directly involved in the emergence of drug resistance by disrupting &/or delaying apoptotic program and promoting tumor survival. Objectives: To analyze the significance of any variation in the level of Bcl-2 expression according to disease progress (from time of diagnosis to time of remission or resistance) in cases with acute leukemia, and whether to consider this variation as a prognostic marker.Methods: Through a prospective study, 25 samples of bone marrow aspirates were taken from different patients who attended the hematology unit of Baghdad teaching hospital in the medical city from December 2005 to July 2006, who proved to have acute leukemia in its different types or subtypes(ALL and AML) & at a variable phases of the disease course. Monoclonal antibody targeted against Bcl-2 oncoprotein via immunocytochemistry staining techniques of these aspirates was performed in order to define the level of Bcl-2 protein carrying cells &looking for any differences in this expression in relation to disease progress or treatment hoping to translate these findings in terms of prognosis.Results: Despite the heterogenous expression of Bcl-2 protein carrying cells in each case(0-500 cells out of 500 cells)of AML or ALL , it was found to be in positive correlation with the density of blast cell percentage in the marrow(r=0.515,p=0.008) unlike the relation with total leucocyte count (r=0.364,p=0.074). Conclusion: A good clinical clue to intractable resistant course of the disease at diagnosis is verified in this study when very high level of Bcl-2 protein carrying cells was found.This finding may have a useful clinical application through the recommendation to change therapeutic strategy, at time of diagnosis, to more aggressive protocols, which is agreed by others.There was no chance to study the application of high level of Bcl-2 protein in terms of long-term survival due to short follow up.


Article
Immunohistochemical study of BCL-2, PCNA and VIMENTIN markers in oral and laryngeal squamous cell carcinoma (Comparative study)

Authors: Shaimaa F. Muhsin شيماء محسن --- Kadhim A. Alsodani كاظم السوداني
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2011 Volume: 23 Issue: special issue Pages: 82-86
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Squamous cell carcinoma (SCC) may arise in any stratified squamous epithelium or muosa that hasundergone squamous metaplasia. The aims of the study were immunohistochemical evaluation and comparison ofPCNA, BCL-2 and VIMENTIN expressions in oral and laryngeal squamous cell carcinoma and correlating suchexpressions with the clinicopathological behavior in both sites.Materials and Methods: This study was performed on thirty formalin-fixed, paraffin-embedded pathologicallydiagnosed oral and laryngeal SCC blocks for the period of June 2006 till July 2010.Age, sex, site and histologic gradeswere recognized. The samples were immunohistochemically stained with monoclonal antibodies toBCL-2, PCNA andVIMENTIN.Results: The age of the patients with squamous cell carcinoma of the oral cavity was between 40 and 86 andbetween 32 and 66 years in larynx. The male/female ratio was 10/5 and 8/7 for oral and Larynx respectively. Themajority of the cases of Oral squamous cell carcinoma were located on the buccal mucosa, Whereas Laryngealsquamous cell carcinoma cases were distributed on glottic mainly..No significant statistical difference in the age andgender between the two groups .Histological grading was recognized for each case of oral and laryngeal squamouscell carcinoma. Two thirds of the oral cases were well differentiated. While 8 of the fifteen cases were moderatelydifferentiated SCC. Regarding histological grading there was no statistically significant difference between the twogroups .PCNA expressed in 14 of the fifteen oral cases whereas in laryngeal, 12 cases were positive. Collectively, 9 ofthe 30 cases were positive for vimentin antibody with different score values,.Bcl-2 was expressed in one case onlyfrom fifteen immunostaining laryngeal cases and all the oral squamous cell carcinoma cases were negative Nosignificant relationship in immunoexpression of he above markers between the tow groups.Conclusions: The results of this study proved that the biological behavior namely the PCNA, VIMENTIN and BCL-2activities was comparable between SCC of the oral cavity and larynx with a spectrum of clinical behavior, due tothe differences in location

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