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Article
Prevalence and Molecular Characterization of Fim H Gene in Escherichia Coli Isolates Recovered From Patients With Utis

Author: Narmin Saeed Merza
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 3 Pages: 470 -477
Publisher: Babylon University جامعة بابل

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Abstract

In this study, the prevalence of fim H gene was studied among 105 E. coli isolates obtained from urine samples of patients attended Azadi hospital in Duhok City. The intended gene was detected in94.3% of the isolates. Triplex PCR assay was applied and according to which the studied isolates were assigned into four groups namely A, B1, B2, and D groups which constituted 20.95 %, 3.8 %, 54.28 %, 20.95 %, respectively. Ten randomly selected isolates were subjected to SNPs fimH analysis with 3 reference strains of E. coli. The results revealed that 44 SNPs observed at 42 polymorphic sites accounting for 5.59%. All mutations were of substitutions and 29.5 % of mutations were transversions while transition type mutations constituted of 70.5 %. Ten SNPs accounting for 22.7 % of mutations gave rise to amino-acid changes (sense mutation) while the rest 34 (77.3 %) resulted in silent mutations. Moreover, twelve SNPs were singletons and among them five were with amino acid replacements. Amino acid replacements due to SNPs accounted for 1.27% of whole sequenced fragment of fimH.It can be concluded that there is no relationship inferred between the isolates of E.coli when the two phylotyping techniques are compared but the results of both can serve the purpose of genotypic characterization of uropathogenic E. coli.


Article
Detection of SCN1A Gene Polymorphisms in Epilepsy Children

Author: Laith Abdul Hassan Mohamed-Jawad1
Journal: Almuthanna Journal of Pure Science (MJPS) مجلة المثنى للعلوم الصرفة ISSN: 22263284 Year: 2017 Volume: 4 Issue: 2
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

Febrile seizure is a common convulsions occur in children from 5 months to 6 years of age whose sufferingfrom high temperature. A total of 50 children with febrile seizures and 50 normal control are included in this study.RFLP used to identify the A/G polymorphisms of the SCN1A gene on chromosome 2q24. Results showed that thegenotype proportions and allele frequencies for SCN1A c.3184 A/G in both groups are not significantly. Proportions ofA homozygote, A/G heterozygote, and G homozygote for SCN1A c.3184 A/G were as follows: in patients with FSs,20%, 50%, and 30%, respectively compared to controls (24%, 62%, and 14%, respectively). The allele A and Gfrequencies for SCN1A c.3184 A/G in patients with FSs was 45% and 55% respectively compared to control group. Thefrequency of AG genotype of SCN1A c.3184 A/G polymorphism was non-significant in FSs compared to control group.There was increased in glucose, phosphorus levels and decrease in sodium, chloride levels. The results do not suggestthat SCN1A p. Thr 1067 Ala or c.3184A/G (rs2298771) are susceptibility factors for febrile seizures and the fever playsan important role in causing disturbances in electrolyte balance.


Article
Association between Resisten Gene Polymorphism (420C/G) and Lipid profile in Ischemic Heart Diseases

Authors: Asraa Ali Kadhum --- Majid Kadhum Hussain --- Riyadh. Dh. Al-zubaidi
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2018 Volume: 11 Issue: 1 Pages: 3937-3942
Publisher: Kerbala University جامعة كربلاء

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background: (resistin is considered as a pro-inflammatory molecule and plays a role in the inflammatory response that lead to atherosclerosis)Aim: To evaluate the risk of resisten gene polymorphism (420C/G) in the development of Ischemic Heart Disease. To verify the relationship of the investigated SNPs with the metabolic changes related to (IHD), in particular, serum lipid profile.Methods: A case control study was performed at which 150 patients with IHD and 150 healthy individuals. Genotyping for SNP 420C>G in the resistin gene was performed by the polymerase chain reaction –restriction fragment length polymorphism method. Lipid profile were measured.Results: The genotype and allele frequencies of resistIn gene polymorphism in IHD and control persons were examined under the co-dominant, dominant and recessive models with the use of multi nominal logistic regression analysis. Neither genotype distribution nor the minor allele frequency showed significant changes among the comparison of the of IHD patients with the control group. The frequency of the G allele of 420(CG) polymorphism was significantly higher in ischemic heart diseases (IHD). There are significant increases in the level of LDL, triglyceride, VLDL, BMI and a significant decrease in the level of cholesterol in the group of patients with the GG+CG genotypes when they were compared with those of the CC genotype. It shows significant differences in BMI, Age, Cholesterol, LDL, and HDL in the group of IHD, and no significant difference was seen in VLDL and sex.Conclusion: The −420C>G SNP of resistin gene is not associated with ischemic heart disease in the population of Kerbala and Najaf. The G allele is seemed to increase serum lipid concentrations so it could be considered as an atherosclerotic parameter.


Article
Association between the Low Density Lipoprotein Receptor (A370T) Gene Polymorphism with Lipid Profile in Type 2 Diabetes Mellitus

Authors: Marwa Ali Zghair --- Fadhil Jawad Al-Tu’ma --- Hassan Mahmoud Abu Al- Maali
Journal: karbala journal of pharmaceutical sciences مجلة كربلاء للعلوم الصيدلانية ISSN: 70272221 Year: 2018 Issue: 15 Pages: 103-112
Publisher: Kerbala University جامعة كربلاء

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Abstract

Background: determining the genetic profile of diabetic patients with dyslipidemia is consideredimportant in terms of reducing the risk of possible vascular complications. Recent studies haveshown that the low density lipoprotein receptor gene may have a direct effect on lipids level and oneof its important genetic variants is the common single nucleotide polymorphism (A370T) that’sproduced by a guanine to adenine substitution in exon 8 in the epidermal growth factor precursorhomology region.Aim: To evaluate the association between the low density lipoprotein receptor (A370T) singlenucleotide polymorphism with lipid Profile in type 2 diabetic patients with dyslipidemia.Method: A case control study for total 180 subjects. 120 patients with type 2 diabetes mellitus (80males and 40 females) and 60 patients of them were diagnosed previously with dyslipidemia, thesediabetic patients were compared with 60 (40 male and 20 female) healthy control subjects. Theblood sample collection were done at the outpatient department of Al-Imam Al-Hussein (as) MedicalCity in holy Karbala/Iraq during the period from January to June 2017.Results: The results obtained demonstrate that there is no relationship between the low densitylipoprotein receptor (A370T) single nucleotide polymorphism and dyslipidemia in patients withtype 2diabetes mellitus with no significant association between this polymorphism and the lipidprofile in these patients .Conclusion: In the current study, the genotyping analysis results agreed with much of studies whichrevealed that there is no relationship between the low density lipoprotein receptor ( A370T) singlenucleotide polymorphism and lipid levels in patients with type 2diabetes mellitus with and withoutdyslipidemia.

الخلفية:ان تحديد الشكل الجيني للمرضى المصابين بالسكري واضطراب شحوم الدم يعتبر من المعايير المهمة في تقليل خطر الاصابةبمضاعفات الاوعية الدموية لدى هؤلاء المرضى. وقد اظهرت الدراسات الجينية الحديثة بأن جين مستقبلات الدهون منخفضة الكثافةوالذي ينتج من (A370T) له تأثير مباشر على مستويات الدهون في الجسم وأحد اهم اشكاله الجينية هي تعدد الشكل النيوكليوتيدياستبدال القاعدة النتروجينية الكوانين بالادينين في الموقع 8 من منطقة التماثل للعوامل البادئة .الهدفتقييم الارتباط بين تعدد الشكل النيوكليوتيدي لمستقبلات الدهون منخفضة الكثافة مع مستويات الدهون للمرضى المصابين بالنوعالثاني لداء السكري واضطراب شحوم الدم.الطريقةاجريت الدراسة على 180 شخص, منهم 120 مريض بالنوع الثاني لداء السكري و 60 مريض منهم مصاب باضطراب شحوم الدمبالاضافة للسكري , هؤلاء المرضى تمت مقارنتهم مع 60 شخص سليم. تم جمع عينات الدم في مستشفى الامام الحسين عليه السلام. التعليمي في محافظه كربلاء المقدسة للفتره من شهر كانون الثاني الى حزيران لسنة 2017النتائجتتضمن النتائج عدم وجود علاقة بين تعدد الشكل النيوكليوتيدي لمستقبلات الدهون منخفضة الكثافة ومستويات الدهون في الاشخاصالمصابين بالنوع الثاني من داء السكري واضطراب شحوم الدم.الاستنتاجفي الدراسة الحالية ,نتائج التحليل الجيني تتفق مع اغلبية الدراسات السابقة والتي تنص على عدم وجود علاقه بين تعدد الشكلالنيوكليوتيدي لمستقبلات الدهون منخفضة الكثافة ومستويات الدهون في الاشخاص المصابين بالنوع الثاني من داء السكري مع اوبدون اضطراب شحوم الدم.


Article
Specific activity of thymidine nucleotide biosynthetic enzymes in hairy roots extracts of Sesamum indicum L. transformed by two strains of Agrobacteruim rhizogenes
الفعالية النوعية لأنزيمات بناء نيوكليوتيد الثايمين في مستخلصات الجذور الشعرية في السمسم المحَولة وراثيا باستخدام سلالتين من Agrobacteruim rhizogenes

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Abstract

The study concluded induction of transformed hairy roots from leaves and decapitated seedlings of Sesamum indicum L. using two strains of Agrobacterium rhizogenes considered as natural vector of transformation. The strain R1601 stimulated roots on leaves and seedlings during 20 days of inoculation placed on solidified Arnon and Hoagland medium. Whereas they involved 12 days when inoculated with the strain R15834. Generally strain R15834 was efficient in inducing these roots and their numbers than strain R1601 which approached 54.4% and 41.6% respectively. The results indicated an increase in the specific activity of enzymes Thymidlate synthase (TS), Dihydrofolate reductase (DHFR), Serine hydroxy methyl transfrase (SHMT) in extract of transformed hairy roots producing agropine by stain R15834 and approach 4.610, 1.057, 0.480 µmolmin mg of protein respectively compared with the activity of 1.256, 0.097, 0.125µ molmin mg in the control samples. This was coupled with an increase in amount of DNA and RNA that approached 105, 1020 µg per gram respectively compared to 44, 462 µg per gram in control samples. The transformation of these hairy roots was pointed out through the separation of agropine spots from their extracts when electrophoreted in the presence of standared agropine.

تضمنت الدراسة استحثاث الجذور الشعرية المحَولة وراثيا من الأوراق والبادرات المزالة مجاميعها الجذرية المعقمة للسمسم Sesamum indicum L. باستعمال السلالتتين601R15834, R1 من بكتريا Agrobacterium rhizogenes باعتبارها نواقل لإحداث التحول الوراثي. شجعت السلالة R1601 نشوء هذه الجذور على الاوراق والبادرات خلال 20 يوما من تلقيحها ونموها على وسط ارنون وهوكلاند الصلب , في حين أستلزم ظهورها على الاوراق والبادرات عند تلقيحها بالسلالة R15834 (12( يوما . وعموما فأن السلالة R15834 كانت الاكفأ في استحثاث الجذور الشعرية واعدادها المتكونة قياسا بالسلالة R1601 وسجلت نسبة تكوينها 54.4% و41.6% على التوالي . وأظهرت البيانات عن زيادة واضحة في الفعالية النوعية لإنزيمات الثايميدليت سنثيز (TS)الداي هيدروفوليت ريدكتيز ((DHFR والسيرين هيدروكسي ميثايل ترانسفريز(SHMT) في مستخلصات الجذور الشعرية المنتجة للاكروبين بوساطة السلالة R15834 مسجلة 4.610 و 1.057 و 0.480مايكرومول/ دقيقة/ملغم بروتين على التوالي قياسا بـفعاليتها 1.256 و0.097 و0.125 مايكرومول/ دقيقة/ملغم بروتين في عينات المقارنة. وقد رافقها زيادة في كمية الاحماض النووية DNA و RNA وبلغت 105 و1020 مايكروغرام/غرام على التوالي قياساً بنظيراتها البالغة 44 و462 مايكروغرام /غرام في عينات المقارنة. واستدل على التحول الوراثي لهذه الجذور من انفصال الاكروبين من الجذور الشعرية لهاتين السلالتين عند ترحيل مستخلصاتها بوجود الاكروبين القياسي.


Article
ASSOCIATION BETWEEN ASN142ASP GENETIC POLYMORPHISM OF GSTO2 AND SUSCEPTIBILITY TO BLADDER CANCER

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Abstract

Background:The glutathione-S-transferases (GSTs) comprise a class of enzymes that detoxify carcinogenic compounds by conjugating glutathione to facilitate their removal. Polymorphisms in glutathione S-transferase Omega 1,2 (GSTO1, GSTO2), and GSTP1 genes have been related to risk for bladder cancer.Objective:To assess a comprehensive picture of the relationship between smoking and GSTO2 gene Asn142Asp variant (rs156697) with bladder cancerMethods:A case control study was conducted at Chemistry and Biochemistry Department, College of Medicine and DNA Research and Training Center, Al-Nahrain University from February 2014 to September 2014. Forty one bladder cancer patients and 41 age matched apparently healthy controls were participated in this study. Genotyping of the GSTO2 Asn142Asp polymorphism was evaluated using a polymerase chain reaction fragment length polymorphism (PCR-RFLP) method. The odds ratio (OR) and 95% confidence interval (CI) were calculated as a measure of the combined effect of cigarette smoking and the GSTO2 Asn142Asp polymorphism on bladder cancer risk.Result:It was found that subject with the GSTO2 Asp/Asp genotype have significantly increased bladder cancer risk (OR 4.92; 95% CI =1.32 - 18.30). A statistically highly significant increased the bladder cancer risk was also found in ever smoker of the GSTO2 (Asn/Asn) (OR =11.8; 95% CI=2.43 - 57.84) and (Asn/Asp +Asp/Asp) (OR =12.8; 95% CI=3.23 - 51.41) compared with never smoker Ala/Ala genotype.Conclusion:The study suggests that smokers having GSTO2 Asn/142Asp polymorphism could play an important role as risk factor for the development with bladder cancer.Keywords:Bladder cancer, single nucleotide polymorphism, glutathione S-transferase, GSTO2, Asn142Asp, smoking, rs156697.


Article
Effect of IL-17Single Nucleotide PolymorphismsGene on the Risk of Developing Colorectal Cancer in Iraqi Patients

Author: Haidar J. Muhammed1, Nassir N. Mohsun2,Aroub A.R. Al-Kaisi2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 2 Pages: 51-58
Publisher: Baghdad University جامعة بغداد

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Abstract

Interleukin -17 pathway plays an important role in development of inflammatory bowel diseases (IBD). Moreover it has been shown that this interleukin involved in anti-tumor or pro-tumor of colorectal cancer (CRC). This study determined (70) patients male to female (60% - % 40)inflicted with colorectal cancer,range aged (<16-80) years which were collected from Baghdad Teaching Hospitals/ Medical City.The patients detected for anti – IL17 by enzyme linked immune sorbent assay (ELISA)and compared with (10) individuals as a healthy control.The results showed significant differences (p<0.05). The gene encoding for the cytokine IL17 has genetically polymorphic, which has (30%) single nucleotide polymorphisms (SNPs) (12out of 40) patients by single strand conformation polymorphism technique (SSCP). In conclusion, polymorphic gene IL17 has been revealed to be associated with its susceptibility of colorectal cancer by showing there was a relationship between polymorphic gene IL17and the occurrence of colorectal cancer as well as the clinical features of the disease especially in agedpatients.


Article
The Impact of MicroRNA Gene Polymorphisms on Immune Response to Diphtheria Vaccine in Iraqi Children and Adolescents

Author: Ghaidaa Jihadi Mohammeda
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2018 Volume: 26 Issue: 6 Pages: 21-30
Publisher: Babylon University جامعة بابل

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Abstract

Background:Sinceitsinventioninthefirstquarterofthelastcentury,diphtheriatoxoidwas successfully used for vaccination against diphtheria. However, sporadic case and evenan epidemichavebeenrecordedinmanypartsoftheworld,suggestingthepresenceofgenetic factors influencing the immune response to thisvaccine.Aim: To assess the role of three single nucleotide polymorphisms(miRNA-146a G> C, miRNA-49T>GandmiRNA-196a2C>T)inmiRNAgenesinimmuneresponsetodiph- theriavaccine.SubjectsandMethods:Thisstudyrecruited68childrenandadolescentswhoreceived threedosesofdiphtheriatoxoid.DNAwasisolatedfromthebloodlymphocytesandmiRNA- 146a,miRNA-149 and miRNA-196a2 genes were amplified, and then directly sequenced. Serumlevelsofanti-diphtheriatoxoidsIgGantibodiesweredeterminedbyenzymelinked immunosorbent assay, and accordingly the immune response was categorized into good, moderate and low immunity .The association of different genotypes and alleles with immune statuswasillustratedstatistically.Results:Goodimmuneresponsetodiphtheriavaccinewassignificantlyassociatedwith the C allele of the polymorphism miRNA-146a G> C. Out of 14 carriers of GG geno- type,11(78.57%)showedgoodimmunitycomparedtoonly48.15%ofGGcarrierswhohad suchimmunity(P=0.039).ThemutantalleleofmiR-149T>Gwasmoreprevalentthanwild typealleleGamongsubjectswithgoodimmunity.However,thedifferencewasinsignificant.GenotypesandallelefrequenciesoftheSNPmiR-196a2C>Twereverycloseindifferent immunologicalstatuses.Conclusion:TheresultsofthecurrentstudysuggestthesignificantroleofmiRNA-146a G>CgenepolymorphismandtheinsignificantroleofbothmiRNA-149T>GandmiRNA- 196a2 C>T gene polymorphisms in response to diphtheria toxoids vaccine. More studies arerequiredtofindouttheassociationsofdifferentpolymorphismsinmiRNAgeneswith immune response for differentvaccines.

منذ ان تم اختراعه في الربع الاول من القرن الماضي استخدم ذيفان الدفتريا المقتول بنجاح في التلقيح ضد مرض الخناق ومع ذلك فقد سجلت حالات فردية بل وحتى اوبئة في اجزاء عديدة من العالم من ما يشير الى وجود عوامل وراثية توثر على الاستجابة المناعية لهذا اللقاح. هدفت الدراسة الحالية الى تقييم دور ثلاث تغايرات جينية (miRNA-146aG>C ، miRNA-149T>G، (miRNA-1962aC>Tفي جين MicroRNA في الاستجابة المناعية ضد لقاح الدفتريا. استخدمت هذه الدراسة 68 طفلا ومراهقا ممن تلقوا ثلاث جرع من لقاح الدفتريا . تم عزل من خلايا ومضاعفة جين MicroRNAالمناظر للتغايرات الجينية المطلوبة ، ومن ثم اجراء اختبار تتابع القواعد . حددت المستويات المصلية للاجسام المضادة بطريقة الادمصاص المناعي الانزيمي وعلى ضوء ذلك صنفت الاستجابة المناعية الى جيدة , متوسطة او منخفضة ، وتم تحديد علاقة الانماط الجينية والاليلات المختلفة بمستوى المناعة باستخدام الطرائق الاحصائية . ارتبطت المناعة الجيدة ضد لقاح الدفتريا معنويا بالاليل C للتغاير الجيني MicroRNA , ومن بين 14 فردا يحملون النمط الجينيCG اظهر 11 ((%78.57 استجابة مناعية جيدة مقارنة ﺑ %48.15 فقط مما يحملون النمط الجيني GG (P=0.039) وكان الاليل الطافر(G) للتغاير الجيني miRNA-149T>G اكثر اتشارا من الاليلT بين الافراد ذوي المناعة الجيني الا ان الفرق لم يكن معنويا . اما توزيع الانماط الجينية والاليلات للتغاير فقد كان متقاربا جدا في الحالات المناعية المختلفة .تشير نتائج الدراسة الحالية إلى دور مهم لتغاير جين miRNA-146aG>Cودور غير مهم لكلا من تغايرات جين miRNA-149 T>G و miRNA-196a2 C>T في الاستجابة المناعية للقاح الدفثريا , ويجب إجراء المزيد من الدراسات للكشف عن علاقة التغايرات الجينية المختلفة في جينات MicroRNA مع الاستجابة المناعية للقاحات المختلفة.


Article
Polycystic Ovary Syndrome and related Some Genetic Markers

Author: Enas Abdul Kareem Jabbar
Journal: Journal of Education for Pure Science مجلة التربية للعلوم الصرفة ISSN: 20736592 Year: 2018 Volume: 8 Issue: 3 Pages: 129-150
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

AbstractPolycystic ovary syndrome (PCOS) is the most familiar endocrine disorder affecting women ofchildbearing could be happen with problem and metabolic anomalies. PCOS women present withovulatory dysfunction, abnormal hormones, hyperandrogenemia, obesity, and hyperinsulinemia.Patients with PCOS may present complaining of irregular or unpredictable menstrual cycles,undesirable hair growth, acne , scalp hair loss, unexplained weight gain or overweight,and infertility.Clinical phenotyping of PCOS involves determining medical and/or biochemical androgen excess. Thebulk of evidence points to the ovary being the source of excess androgens, which appears as a resultof an abnormal regulation of steroidogenesis.It is a heterogeneous disorder which results from interaction of multiple genes and environmentalfactors.


Article
Single‑Nucleotide Polymorphism of Interleukin‑27 Gene: A Risk Factor of Recurrent Pregnancy Loss in Iraqi Women

Authors: Esraa H. Humadi --- Layla H. Hamad --- Hasan F. Al Azzawie --- Samera H. Hamad
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2018 Volume: 17 Issue: 1 Pages: 47-51
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Background: Studies have been done to investigate the association between a single‑nucleotide polymorphism (SNP) of interleukin‑27 (IL‑27)gene and the recurrent pregnancy loss (RPL). However, different results have been found in different spots of the world. Therefore, more studies are needed to understand the variation in these results. This is the first study that shows the implication of the SNP of IL‑27 gene in RPL. Objective: This study aims to investigate the association between RPL and SNP of gene (−964 A > G) in Iraqi women.Materials and Methods: From September 2013 to September 2014; 100 women, as a control group, and 100 women (with three or moreconsecutive pregnancy loss), as a study group, were recruited to investigate the association between the IL‑27(−964 A > G) SNP and the PRL. The IL‑27(−964 A > G) SNP was determined using polymerase chain reaction‑restriction fragment length polymorphism technique. Genotypeand allele frequencies were compared using Fisher test between the two groups. P < 0.05 is considered to be statistically significant. Results: The age and body mass index were both not significantly different between the two groups. The frequencies of genotypes of this polymorphism in the RPL group were AG (60%), AA (31%), and GG (9%), while these frequencies were AG (21%), AA (68%), and GG (11%) in the control group.The genotype frequencies of the −964 A > G polymorphism was significantly different between the study and the control groups (P = 0.007). The allele frequencies of this polymorphism were A (35%), G (65%) in the RPL group versus A (61%), G (39%) in the control group. The frequencies of A and G alleles in the both groups were not significantly different. Conclusion: IL‑27 (−964 A > G) polymorphism is a risk factor for RPL in a sample of Iraqi women. However, this is different from what has been found in some studies which might implicate other factors in the RPL.

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