research centers


Search results: Found 13

Listing 1 - 10 of 13 << page
of 2
>>
Sort by

Article
دراسة تأثير دعائم التقييد في ثباتية انزيم اليوريز المنقى من بذور الحنظل Citrullus colocynthis

Loading...
Loading...
Abstract

Abstract This study revealed that urease was immobilized by entrapment in gelatin and calcium alginate, the ratios of immobilization were 89% and 80% respectively. The result of immobilized urease characterization showed that the enzyme retained its original activity when it was incubated at 70 ºC for 50 minutes. The maximum enzyme activity of immobilized enzyme was 80 ºC and the optimum pH of activity was 7.5.The study revealed that the Km and Vmax of the immobilized enzyme were 181 mM and 225 mM/Min. respectively, when the urea was used as a substrate.As for the stability of storage for free and immobilized enzyme, the study showed that the immobilized enzyme by gelatin lost 20% of its activity after 90 days from keeping in phosphate buffer 50 mM, pH 8 at 4 ºC in comparison with free enzyme which lost 50% under the same condition. The enzyme immobilized by gelatin retained its original activity till the tenth use while the enzyme immobilized by calcium alginate retained its original activity till the fourth use.

الخلاصهافادت الدراسة الى امكانية تقيد الانزيم المنقى من بذور الحنظل باستعمال الجيلاتين والجينات الكالسيوم اذ كانت نسبة التقيد 89% و80% على التوالي. وبينت نتائج توصيف اليوريز المقيد بالجيلاتين احتفاظ الانزيم المقيد بكامل فعاليته عند حضنه تحت 70 م لمدة 50 دقيقة. كما لوحظ ان اقصى فعالية للانزيم المقيد كانت عند درجة 80 م. وكانت قيمة الرقم الهيدروجيني الامثل لفعالية الانزيم المقيد 7.5 وعند دراسة الثوابت الحركية لليوريز المقيد، ظهر ان معدلات قيم ثابت ميكالس (Km) والسرعة القصوى Vmax كانت 181 ملي مولار و225 ملي مولار/دقيقة على التوالي عند استخدام اليوريا مادة اساس. وعند دراسة ثباتية الخزن للانزيم الحر والمقيد أوجدت الدراسة ان الانزيم المقيد بالجيلاتين يفقد 20% من فعاليته بعد 90 يوماً من حفظه في محلول الفوسفات الدارئ بتركيز 50 ملي مولار ، وبرقم هيدروجيني 8 ، وبدرجة حرارة 40 م قياساً بالانزيم الحر الذي يفقد 50% من فعاليته بالظروف نفسها اعلاه. كما احتفظ الانزيم المقيد بالجيلاتين بكامل فعاليته عند الاستخدام العاشر ، بينما أحتفظ الانزيم المقيد بالجينات الكالسيوم بكامل فعاليته لحد الاستخدام الرابع.

Keywords


Article
تنقية وتوصيف انزيم اليوريز الحر المستخلص من بذور نبات الحنظل كمصدر جديد

Authors: محمد عبد الله الخفاجي --- مفيد جليل عوض --- غازي منعم عزيز
Journal: journal of kerbala university مجلة جامعة كربلاء ISSN: 18130410 Year: 2007 Volume: 5 Issue: 3 Pages: 1-14
Publisher: Kerbala University جامعة كربلاء

Loading...
Loading...
Abstract

Keywords


Article
Immobilization of urease in gelatin beads for urea estimation

Loading...
Loading...
Abstract

Abstract Urease was immobilized in gelatin beads. The optimum immobilization (79% activity) was observed at 4 C˚ with protein concentration of 1.0 mg/ml. the apparent optimum pH shifted from 7.5 to 6.5. Immobilized urease showed optimum activity at 60 C˚ compared with 40 C˚ for the soluble urease.The immobilized urease remained very active over long period of time and this enzyme lost about 40% of its original activity over the period of 60 days for storage at 4C compared with soluble urease which lost about 75% its activity in the same conditions. Blood urea estimation is carried out with immobilized enzyme beads compared with soluble enzyme and the beads can be used repeatedly for this purpose making it economical procedure compared to standard chemical method.

الخلاصةتم تقيد أنزيم اليوريز باستخدام الجيلاتين وكانت نسبة التقيد 79% اظهر الأنزيم المقيد أعلى فعالية عند رقم هيدروجيني 6.5 مقارنة بالأنزيم الحر7.5. واظهر أعلى فعالية عند درجة حرارة 60 ˚م مقارنة بالأنزيم الحر (40 ˚م).بقي الأنزيم المقيد فعال لفتره طويله حيث فقد حوالي 40% من فعاليته عند خزنه لمدة 60 يوم مقارنة بالأنزيم الحر الذي فقد 75% من فعاليته عند خزنه بنفس الظروف. تم تقدير نسبة اليوريا بالدم باستخدام الأنزيم المقيد وكانت الطريقة كفوءة واقتصادية.

Keywords


Article
ADENOSINE 3' , 5' – CYCLIC MONOPHOSPHATE : EXT RACION AND PURIFICATION FROM URINE OF β – THALASSAEMIA PATIENT

Loading...
Loading...
Abstract

Abstract Cyclic adenosine 3',5'-monophosphate has been extracted and purified using column chromatography ( Dowex 50-w column of 1.5x 5 cm dimentions ). Levels of the purified adenosine cyclic monophosphate peak was determined and it was seen to be elevated in urine of thalassaemic patients in comparison with that of healthy controls objects. The purified peak was identified using UV analysis

الخلاصة تم استخلاص وتنقية الادينوسين 3',5' الاحادي الفوسفات الحلقي باستخدام عمود الكراماتوغرافيا (D50-w) وبقياسات 1.5x 5 سم . وتم تحديد مستويات الادينوسين احادي الفوسفات الحلقي بتحديد المنحنى الخاص به باستخدام تقنية الاشعة فوق البنفسجية وتبين بان مستوياته ترتفع في نماذج البول المأخوذة من مرضى الثلاسيميا مقارنة مع الاصحاء .

Keywords


Article
Hormonal Contents of Two Types of Black Seed (Nigella sativa) Oil: Comparative Study

Authors: Abdulsamie H. Alta'ee عبدالسميع حسن الطائي --- Mufeed J. Ewadh مفيد جليل عوض --- Haider K. Zaidan حيدر كامل زيدان
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2006 Volume: 3 Issue: 1-2 Pages: 17-21
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

The use of black seed (Nigella sativa) capsules as a herbal therapy to lower blood glucose in diabetic patients are associated with menstrual cycle irregularities. The present study was designed to analyses and to compare between two types of Nigella sativa (N.sativa) agricultures in Mosul region and those imports from Saudi Arabia Kingdom, and to investigate the levels of hormones in volatile oil of black seed that regulate menstrual cycle that might be present in Nigella sativa. Volatile oil of N.sativa was extracted by steam distillation using diethyl ether as an organic solvent.Then, hormones analysis were done using (miniVIDAS software version) instrument. Results of present study showed a presence of considerable amount of sex hormones estradiol, progesterone, prolactin, testosterone, FSH and LH .This might interpret the cause of menstrual cycle irregularities occur when black seed capsule was taken as a herbal therapy to lower blood glucose in diabetic patients.

Keywords


Article
The Correlation between Lipid Profile and Lipid Peroxidation in Patients with Acute Myocardial Infraction

Authors: Mufeed J. Ewadh مفيد جليل عوض --- Abdulsamie H. Alta'ee عبدالسميع حسن الطائي --- Husain S. Aljanabi --- Alaa Hummady Alta’ee
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2005 Volume: 2 Issue: 3 Pages: 301-306
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

To measure the association between risk factor of cardiovascular disease (CVD) and lipid peroxidation in patients with acute myocardial infraction (AMI) and healthy controls, twenty six patients (17 males, 9 females) with AMI clinically diagnosed admitted to Merjan Teaching Hospital in Hilla city and fifty two (25 males ,27 females) apparently healthy persons as a controls subject to present study. Determination of serum total cholesterol (TC), triacylglycerol (TG), high density lipoprotein (HDL)-cholesterol, serum glutathione and serum lipid peroxidation were preformed using colorimetric methods. Very low density lipoprotein (VLDL) and low density lipoprotein (LDL) was determined using mathematically method. TC and TG of males with AMI found to be significantly increased and total cholesterol of females with AMI found to be increased when compared with healthy controls. HDL- cholesterol of patients found to be decreased, whereas risk factor was significantly increased. VLDL of patients with AMI found to be significantly decreased, whereas LDL found to be significantly increased. Lipid peroxidation was increased, but serum glutathione was declined significantly in patients with AMI when compared with healthy controls. The correlation between lipid peroxide and TG shows a positive relationship of oxidation product excretion at higher levels of TG. We conclude that patients with elevated levels of serum TG and cholesterol are at increased risk of oxidative damage due to lipid peroxidation.

Keywords


Article
Cytidine Deaminase Activity In Breast Cancer
فعالية السايتدين دي امينيز في سرطان الثدي

Authors: Oda M. Y. Al-Zamely --- Mufeed J Ewadh مفيد جليل عوض --- Abdulsamie H. Alta'ee عبدالسميع حسن الطائي
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2004 Volume: 1 Issue: 1 Pages: 32-39
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Cytidine deaminase (CTD) catalyzed the deamination of cytidine (CR) to uridine (UR) is a cytoplasmic enzyme existing in many kind of cells and widely distributed among mammalian tissues. It has beel1 reported to be a sensitive indicator in several types of abnormalities. CTD activities in sera of thirty-eight females with breast cancer (BC) and twenty-five healthy females asa contrcl were measued using a spectrophotometric method. Also electophoretic patte.n ofsera of patients with BC and healthy fernales were assayed using polyacrylamide gel electrophoresis(PAGE) to differentiate between normal and abnomal proteins existing in the two cases.Compared with healthy females, CDA activities found to significantly increase in sera of patients with BC (4.96+ 2.34, 13.3 + 3.72 U/L) (P < 0.000) respectively. PAGE profile of sera of patients with BC shows wide variety than that of nomal females.These results may consider a novel biochemical marker to aid in the diagnosis of BC.

Keywords


Article
The Key Role Correlation Of Creatine Kinase Activity and Antioxidants Status in Diabetic Patients Type I and II

Loading...
Loading...
Abstract

AbstractThe activity of creatine Kinase (CK) estimated, in sera of diabetic patients (36 males, 18 females) type I & II and the concentration of antioxidant variables such as Glutathione, uric acid compared with healthy controls has been also estimated, CK activity was found to be significantly decrease in patients with Diabetes Mellitus; while isoenzyme (MM-CK,BB-CK and MB-CK) levels fluctuated between decrease and relatively constant in patients with Diabetes mellitus .Glutathione, uric acid was found to be reduced when compared with those of healthy controls. The depletion in antioxidant concentrations may be due to their protective role against oxidative stress in those patients

الخلاصةتم قياس فعالية أنزيم الكرياتين كاينيزفي أمصال مرضى داء السكري النوع الأول , والثاني (36 ذكور,18 إناث ) اضافه إلى قياس المتغيرات في تراكيز مضادات الاكسده كالكلوتاثايون وحامض اليوريك لتبيان العلاقة بين الطرفين وقد دلت النتائج بان فعالية الإنزيم ومضادات الاكسده تناقصت بشكل ملحوظ في مرضى السكري بينما أظهرت متماثلات الإنزيم تغيرات تراوحت بين ثبوت ونقصان عند نفس المرضى .إن النقصان الحاصل في تراكيز المضادات قد يعود إلى قابليتها على مقاومه الجهد التاكسدي الحاصل في هؤلاء المرضى وبذلك هدفت الدراسة إلى خلق علاقة مهمة بين الإنزيم من جهة وبين المضادات من جهة أخرى للاستدلال على القيم المعنوية لهذه العلاقة البايولوجيه.

Keywords


Article
Lipid profile in Diabetic Children

Loading...
Loading...
Abstract

AbstractHeart problems are an important cause of morbidity and mortality all over the world. High cholesterol is an important cause which might start in childhood The aim of this study is to assess lipid profile as a risk factor for heart disease. Fifty two children with diabetes mellitus type 1 were assessed regarding their lipid profile compared to 37 non diabetic children. The results shows that growth parameters were found lagging behind the normal range for age and sex.Cholesterol and LDL(Low Density Lipoprotein) were found high compared to control group(P<0 .05).

الخلاصةتعتبر أمراض القلب من الأسباب المهمة للوفاة في العالم ويعتبر ارتفاع نسبة الدهون عاملا مهما في ذلك وقد تبدأ الزيادة في الدهون في عمر الطفولة. هدف البحث هو لقياس نسبة الدهون في الأطفال المصابين بالسكر كعامل مؤدي إلى تصلب الشرايين. تمت دراسة اثنين و خمسين طفلا مصابا بالسكر المعتمد على الأنسولين و قياس نسبة الكولسترول و الدهون قليلة الكثافة و قورنت النتائج مع سبع و ثلاثين عينة سيطرة لأطفال أصحاء و كانت الفروقات معنوية(P<0.05)

Keywords


Article
The Behavior of the Plasma Homocysteine and Selenium Concentrations in Patients with Acute Myocardial Infarction
سلوك مستويات الهوموسيستين والسيلينيوم في بلازما دم المصابين باحتشاء العضلة القلبية الحاد

Authors: H. Renz --- S. Mkhlof --- Aamir S. AL-Mu'min --- Abdulsamie H. Alta'ee عبدالسميع حسن الطائي --- et al.
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2008 Volume: 5 Issue: 3-4 Pages: 543-550
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Background: Increased concentrations of plasma total homocysteine and decreased concentrations of plasma selenium are separately associated with cardiovascular disease. Objective: Investigate the correlation between plasma total homocysteine and selenium in patients with acute myocardial infarction (AMI). Methods: Patients of present study was thirty nine men with AMI admitted to Marburg Hospital in Marburg city, Germany on 2007. Plasma total homocysteine was determined using HPLC. Plasma selenium was determined using atomic absorption spectrometry. Results: Plasma homocysteine of patients found to be increased, whereas plasma selenium found to be decreased when compared with reference range . The correlation between plasma homocysteine and selenium found to be negatively correlated. Conclusion: The negative correlation between plasma tHcy and selenium may indicate that they have a significant impact on the process of atherogenesis. The change in the levels of plasma total homocysteine and plasma selenium might be result from oxidative stress associated with AMI.

Keywords

Listing 1 - 10 of 13 << page
of 2
>>
Sort by
Narrow your search

Resource type

article (13)


Language

English (12)

Arabic (1)


Year
From To Submit

2012 (1)

2009 (2)

2008 (3)

2007 (1)

2006 (3)

More...