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Article
Rapid Decontamination of Gutta Percha Cones Using Different Chemical Agents

Authors: Mahmoud Y Taha --- Nawal A AL-Sabawi --- Enas Y Shehab
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2010 Volume: 10 Issue: 15 Pages: 30-37
Publisher: Mosul University جامعة الموصل

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Abstract

Aim: To compare the effectiveness of four different disinfectant solutions in rapid decontamination of gutta percha cones. Materials and Methods: In this experimental study, 320 gutta percha cones were placed in bacterial suspensions of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Can-dida albicans for 30 minutes, and then immersed in disinfectant solutions (6% hydrogen peroxide, 2% chlorhexidine, 1% sodium hypochlorite, and 70% ethyl alcohol) for 1, 5, 10, and 15 minutes. After that, the cones were aseptically transferred to the test tubes containing 4 ml brain heart infusion broth, and then cultured on selected agar media and number of colonies was estimated after 24 hours incubation at 37 oC. Results: Hydrogen peroxide was effective in 1 minute, while chlorhexidine and sodium hypoch-lorite after 10 minutes, and ethyl alcohol in 5 minutes for decontamination of gutta percha cones. Con-clusion: Hydrogen peroxide was the most effective agents in decontamination of gutta perch cones


Article
Antimicrobial Activity of Grapefruit Seeds Extracts (In vitro Study)

Authors: Enas Y Shehab --- Nahla O M Tawfik --- Waidulla N Al–Âni
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 18 Pages: 341-345
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: The purpose of this study is to determine the antimicrobial activity of aqueous and alcoholic extract of grapefruit (Citrus Paradisi Rutaceae) seeds. Materials and Methods: Aqueous and alcoholic (ethanol) extracts of grapefruit seeds "GSE" in 20% (w/v) concentration were investigated for activity against Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumonia, Candida albicans and a mixed oral flora. The level of antimicrobial effects was established using in vitro disc diffusion method. Their antibacterial and antifungal activity was compared to the activity of Chlorhexidine (CHX) solution in two concentrations (0.12 and 0.2%) as a control. Results: The aqueous GSE (20%) solution used in this study gave positive results with lethal effect on the tested organisms with zones of inhibition ranging from 10mm-18mm in diameter, which is comparable to that of chlorhexidine (7mm - 22mm) and (13mm - 20mm) for 0.12% and 0.2% solutions respectively. Ethanolic GSE did not show any antimi-crobial activity. Conclusions: Aqueous GSE has a good antimicrobial effect, which makes it a good natural preparation for use as antiseptic or disinfectant.

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