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Article
The antimicrobial effect of aqueous & alcoholic extracts ofeucalyptus leaves on oral Mutans streptococci, Lactobacilli& Candida albicans (an in vitro study).

Authors: Abbas S. Al-Mizraqchi --- Firas H. Qanbar
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2009 Volume: 21 Issue: 4 Pages: 109-112
Publisher: Baghdad University جامعة بغداد

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Abstract

ABSTRACT
Background: The antimicrobial properties of the plants constituents have been known for many years and have
been used against a wide variety of bacteria and fungi including oral pathogens. The purpose of this research is to
find out and to compare between the antimicrobial properties of eucalyptus leaves aqueous and alcoholic extracts
on the most cariogenic bacteria in mouth (Mutans streptococci and Lactobacilli) and against Candida albicans.
Materials and methods: Mutans streptococci, Lactobacilli & Candida albicans were isolated from 25 saliva samples
from dental students. These isolates were purified and diagnosed according to morphological characteristics and
biochemical tests. Aqueous and alcoholic extracts were prepared from eucalyptus leaves in different concentrations
and estimated in mg/ml. Chlorhexidine 2mg/ml (0.2%) was used in the in vitro experiments while absolute ethanol
used as control. Susceptibility of Mutans streptococci, Lactobacilli and Candida albicans were tested by agar
diffusion technique.
Results: There was statistically highly significant difference (p<0.001) between different concentrations of the aqueous
and alcoholic extracts on the sensitivity of the isolates, whilst the alcoholic extract was more effective than aqueous
extract just at low concentrations. At 100mg/ml and 150mg/ml the alcoholic and the aqueous extracts were better
than the 2mg/ml Chlorhexidine in relation to Mutans streptococci and Candida albicans. Minimum bactericidal
concentration for the aqueous extract was 5-8mg/ml, 6-10mg/ml and 3-7mg/ml for Mutans streptococci, Lactobacilli
and Candida albicans respectively while that of the alcoholic extract was 4-8mg/ml, 6-10mg/ml and 2-6mg/ml in
relation to Mutans streptococci, Lactobacilli and Candida albicans respectively.
Conclusion: Leaves extracts (aqueous and alcoholic) showed antimicrobial action against Mutans streptococci,
Lactobacilli and Candida albicans.
Key words: eucalyptus leaves, extracts, oral mutans streptococci, lactobacilli, Candida albicans. (J Bagh Coll
Dentistry 2009; 21(4): 109-112).

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Article
The Dental Caries Experience in Relation to Salivary Flow Rate, SIgA and Mutans Streptococci Bacteria in Smoker and Non-Smoker Patients

Authors: Ansam Zuhair Najm --- Abbas S. Al-Mizraqchi
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2019 Volume: 31 Issue: 1 Pages: 52-59
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Dental caries is a localized, progressive destructive, largely irreversible microbial based disease of multifactorial nature; these factors include (host, microbes and food) they influence differently on the initiation and progression of dental caries.The aims of the study: was to evaluate the effect of smoking on salivary flow rate, secretory immunoglobulin (SIgA) level and viable count of mutans streptococci (M.S) bacteria in oral cavity and their relation to dental caries experience.Material and method: The samples were collected from 80 male students ranging in ages from 18-22 years old. Where they divided in to two groups, 40 non-smokers (control group) and 40 smokers (study group). Unstimulated salivary samples were collected. Salivary flow rate was estimated and viable count (CFU/ml) of mutans streptococci was determined. The diagnosis and recording of dental caries were done according to WHO, 1987 criteria and the level of SIgA was determined by ELISA.Result: the result revealed that the salivary flow rate and SIgA level were lower in smoker group than non-smoker, while the means value of dental caries experience Decay, Missing and Filling tooth (DMFT) and (CFU/ml)of M.S were higher in oral cavity of smoker group than non-smoker group.Conclusion: the smoking has negative effect on salivary flow rate, SIgA and increase the viable bacterial count of M.S and dental caries in smoker patients.


Article
Effect of ozonated water on adherent Mutans Streptococci (In vitro study)

Authors: Adel Farhan Ibraheem --- Abbas S AL-Mizraqchi --- Hasanain MHabeeB
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2009 Volume: 21 Issue: 1 Pages: 18-23
Publisher: Baghdad University جامعة بغداد

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Abstract

ABSTRACT
Background: The aim was to evaluate the antibacterial efficiency of ozonated water against adherent bacteria (in
vitro).
Materials and Methods: Ten dentin samples per group were inoculated with bacterial suspension and treated as
follows: (I) untreated served as –ve control, (II) sterile distilled water for 10 seconds served as +ve control, (III) 5.25%
sodium hypochlorite (Sultan-USA) for 10 seconds, (IV) 0.2% chlorhexidine (Corsodyle®, England) for 10 seconds, and
(V) 4mg/L ozonated water (Ozonesolution-Enaly, USA) for 10 seconds. Swab taken from all samples and an inoculum
was spread on the selective medium MSB (HiMedia, India). Count of bacteria was recorded expressed in colony
forming unit (CFU) taking in consideration the dilution factor.
Results: There was high significant reduction in viable count of adherent M.S treated with ozonatd water 4mg/L
compared with the other groups at P<0.01 level.
Conclusions: According to the circumstances of this study, ozonated water was very potent antibacterial agent
against mutans streptococci.
Keywords: Ozonated water, Streptococci mutans, adherence. (J Bagh Coll Dentistry 2009; 21(1): 18-23)

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Article
Bacteriological Findings within Internal Implant Hole Following Flapless Implant Placement

Authors: Abbas S.AL-Mizraqchi --- Basima GH. Ali --- Khudair Ali Abd
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2018 Volume: 30 Issue: 3 Pages: 13-16
Publisher: Baghdad University جامعة بغداد

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Abstract

Back ground: Microbial penetration inside the implant's internal hole creates a bacterial reservoir that is related with an area of inflamed connective tissue opposite the fixture-abutment junction and this can affect the health of the peri-implant tissue.Aims of the study: Evaluate the types aerobic and anaerobic bacterial count-percentage and difference between Aerobic and Anaerobic microflora in the implant screw hole three months after implant placement. Monitor the periodontal health status of all patients, throughout the study.Material and methods: Study methodology; Eight partially edentulous patients received 20 dental implants and these implants done with flapless surgical procedure. All patients examined clinically to determined their oral health status by examination of their plaque index, Gingival index and Bleeding on probing, each two weeks for 90 days (8visits) throughout the study period. Three months after that, the plaque sample collected from the internal hole of fixture and transfer to bacterial investigation and assessment the amount of anaerobic and aerobic bacteria.Results: Although the anaerobic viable count is higher than that of aerobic, but with statistically not significant difference between those counts (P>0.05).


Article
Comparative study of apical extrusion of intracanal bacteria using different instruments and techniques (In vitro study)

Authors: Abbas S. Al-Mizraqchi --- Waleed N. Al-Hashimi --- Linz A. Shalan
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2009 Volume: 21 Issue: 2 Pages: 23-27
Publisher: Baghdad University جامعة بغداد

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Abstract

ABSTRACT
Background: To compare the number of bacteria extruded apically from extracted teeth after canal instrumentation
using the nickel-titanium instruments (both Hand & Rotary ProTaper) and hand Stainless Steel instruments.
Methodology: sixty extracted single-rooted human teeth were used. Access opening was prepared and root canals were
then contaminated with a suspension of Enterococcus faecalis. The contaminated roots were divided into three
experimental groups of 20 teeth. Group 1, Rotary ProTaper group: the root canals were instrumented using Rotary
ProTaper instruments. Group 2, Hand ProTaper group: the root canals were instrumented using Hand ProTaper instruments.
Group 3, Hand Stainless Steel instruments group: the root canals were instrumented using Hand Stainless Steel instruments.
Bacteria extruded from the apical foramen during instrumentation were collected into vials. The microbiological samples
from the vials were incubated in culture media for 24 h. Colonies of bacteria were counted and the results were given as
number of colony-forming units. The data obtained were analysed using the one-way ANOVA analysis of variance and ttests
p value 0.05 as the level for statistical significance.
Results: There was no significant difference to the number of extruded bacteria between the Rotary & Hand (P>0.05 Non
significant) but there was significant difference between Rotary & Hand ProTaper and Hand Stainless Steel instruments
(P < 0.05).
Conclusions: Hand Stainless Steel instruments extruded more bacteria apically than Rotary & Hand ProTaper.
Keywords: apical extrusion, bacteria, engine-driven techniques. J Bagh Coll Dentistry 2009; 21(2):23-27)

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Article
The effect of aspartame and saccharin on the antimicrobial activity of chlorhexidine against mutans streptococci

Authors: Fadia Abd – Muhsin --- Zainab A, Al – Dhaher --- Abbas S, Al – Mizraqchi
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2008 Volume: 20 Issue: 2 Pages: 93-97
Publisher: Baghdad University جامعة بغداد

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Abstract

ABSTRACT
Background: Although chlorhexidine is the most effective against dental plaque it is extremely bitter to prepare formulation, it is necessary to use flavoring and sweetening which can inhibit the antibacterial effect of rinse preparation.
Materials and Method: The effect of different concentrations of aspartame and saccharin on the antibacterial effect of chlorhexidine 0.2% against Mutans Streptococci was evaluated using the agar diffusion method and determination of MIC value of chlorhexidine alone and in presence of aspartame or saccharin was also done in this study.
Results: The results of this study demonstrate that at a concentration of 4%, 8%, 12%, 16% aspartame did not significantly inhibit the antibacterial activity of 0.2% chlorhexidine however the antibacterial activity of chlorhexidine significantly reduced when increased the concentrations of aspartame up to 40% and MIC values increased when increased the concentrations of aspartame, while saccharin interfere with the activity of chlorhexidine 0.2% and significantly reduced the anti – mutans activity of chlorhexidine 0.2% at all the concentrations used in this study (6% - 60%) and MIC values increased when increased the concentrations of saccharin.
Conclusion: This in vitro study suggests that aspartame may be used as sweetener and flavoring in concentrations up to 16% with chlorhexidine rinse.
Key words: Chlorhexidine, aspartame, saccharin, mutans streptococci. (J Bagh Coll Dentistry 2008; 20(2):93-97)

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Article
The relationship between periodontal disease and chronic obstructive pulmonary disease associated with smoking

Authors: Abbas S.Al-Mizraqchi --- Khulood A. Al-Safi --- Qais K. Kadhum
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2009 Volume: 21 Issue: 2 Pages: 91-94
Publisher: Baghdad University جامعة بغداد

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Abstract

ABSTRACT
Background: The purpose of this study was to determine the relationship between periodontal disease and chronic
obstructive pulmonary diseases (COPD) in the presence of smoking as a risk factor by evaluating the periodontal
parameters and similarities between infecting microorganisms of the periodontium (dental plaque) and lower
respiratory tracts.
Materials and Methods: A total of 90 patients with COPD were selected from the Chest and Respiratory Diseases
Institute/ Ministry of Health, while healthy subjects selected from Department of Periodontics at the College of
Dentistry / University of Baghdad, all participant of study and control groups were examined in the Department of
Periodontics .
Results: The statistical analysis showed significant differences in plaque index, gingival index, probing pocket depth
when comparing between study groups with the control group. No significant difference was found in the clinical
attachment loss between the two groups although the study group showed higher value. The microbiological
identification showed similarities in the types of aerobic microorganisms which causes the periodontal disease and
COPD exacerbations.
Conclusion: The study concluded that the study group had higher mean values of plaque index, gingival index,
probing pocket depth and clinical attachment loss than the control group, both study and control group showed
the same types of microorganisms in plaque sample and in throat swab and patients with COPD showed lower rate
of salivary flow than those of control group.
Keywords: periodontal disease, chronic obstructive pulmonary diseases, smoking. J Bagh Coll Dentistry 2009;
21(2):91-94)

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Article
Antibacterial efficiency of salvia officinalis extracts and their effect on growth, adherence and acid production of oral Mutans Streptococci

Authors: Hadi A. Hmeem Al-Lamy هادي حميم اللامي --- Abbas S. Al-Mizraqchi عباس المزرقجي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 1 Pages: 153-157
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The use of antimicrobial agent to control plaque and oral disease has been advocated for a number ofyears. Different compounds have been delivered through mouth rinses or tooth pastes or by topical application. Thepurpose of this research is to find out and to compare between the anticariogenic properties of aqueous and alcoholicsage extract on the most causative cariogenic bacteria in the oral cavity (Mutans streptococci).Materials and methods: In the present study Mutans streptococci were isolated from saliva often dental students (agerange between 21-23 yrs) .These bacteria were isolated, purified and diagnosed according to morphologicalcharacteristic and biochemical tests.Results: Agar diffusion technique showed that sage extracts (aqueous and alcoholic) were inhibited the growth ofMutans Streptococci, and the diameter of inhibition zone increased as the concentration of sage extract increased, butthe effect of aqueous extract was less than the effect of alcoholic extract. The minimum bactericidal concentration ofaqueous and alcoholic sage extract were 50%, 20% respectively. Also the alcoholic extract was high significant inhibit(P<0.01) the viable count of Mutans Streptococci in vitro in comparison to aqueous extract.Conclusion: Alcoholic sage extract was interfered with acid production and adherence of Mutans Streptococci higherthan aqueous extract resultant in reducing of acid production and inhibition of the adherence of this cariogenicbacteria; alcoholic sage extract have substantively phenomenon similar to those in chlorohexidine in comparison toaqueous extract

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