research centers


Search results: Found 5

Listing 1 - 5 of 5
Sort by

Article
3.ACUTE AND SUBACUTE TOXICITY OF CHLOROFORM EXTRACT OF XANTHIUM STRUMARIUM LEAVES

Authors: Atheer S. Alsabah اثير صباح عبود --- Abdulkareem H. Abd عبد الكريم حميد عبد --- Ahmed M. Al-Shammari احمد الشمري
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2019 Volume: 17 Issue: 2 Pages: 108-113
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:The incidence of cancer is increasing worldwide. Xanthium strumarium may possess anticancer activity, the plant extract for in vitro anticancer activity against a panel of three human cell lines (Breast MCF7, Renal TK10 and Melanoma UACC62), exhibit anticancer activity against these three human cell lines screened by National Cancer Institute.Objective:To investigate the safety of chloroform extract of Xanthium strumarium leaves, which showed potent cytotoxic activity against tumor cells.Methods:The leaves were dried and grounded into fine powder, and extracted with chloroform, that showed potent cytotoxic activity has been tested against two animal species mice and rats for testing its safety. Toxicity was evaluated in Swiss albino mice by feeding with serial doses of extract between 1.0 to 20.0 gm/kg orally and observed continuously for the first 4 hr and hourly for the next 24 hr, then every 6 hr for 48 hr (72 hr, acute toxicity). Rats were also fed with extract single dose of 5 gm/kg, the toxicity was carried out by assessing the effects on biochemical parameters, body weight and relative organ weights for both male and female rats.Results:LD50 of chloroform extract was 3.07 gm/kg. The biochemical finding showed no significant differences compared to control. No significant weight changes occur throughout the study.Conclusion:Chloroform extract has low LD50 (3.07 gm/kg), but the acute toxicity study showed no mortality or signs of toxicity, with non-significant changes in body weights, relative organ weights, and biochemical tests among treated groups compared to their controls.Keywords:Xanthium strumarium, chloroform extract, cytotoxic herb, acute toxicityCitation:Alsabah AS, Abd AH, Al-Shammari AM. Acute and subacute toxicity of chloroform extract of Xanthium strumarium leaves. Iraqi JMS. 2019; 17(2): 108-113. doi: 10.22578/IJMS.17.2.3


Article
EFFECT OF GLYCYRRHIZA GLABRA ON ANTIGEN INDUCED ARTHRITIS IN MICE MODEL

Loading...
Loading...
Abstract

Background:Rheumatoid arthritis is a chronic inflammatory autoimmune disease represents the most common form of chronic inflammatory joint diseases. Glycyrrhiza glabra (G. glabra) was widely known to have several pharmacological activities, which might be beneficial in preventing and treating both acute and chronic inflammatory conditions.Objective:To study the effect of aqueous extract of G.glabra on antigen induced arthritis model in mice.Methods:Forty-eight male Swiss albino mice were used in this study. Group 1 arthritic mice without treatment (positive control); group 2 arthritic mice treated with G. glabra aqueous extract 750 mg/kg/day; group 3 arthritic mice treated with G. glabra aqueous extract 300 mg/kg/day and group 4 negative control (non-immunized, non-treated mice). Antigen induced arthritis was induced by Methylated bovine serum albumin in Imject Alum adjuvant. The mice were given the drug orally and the treatment was started from day 1 of the induction of arthritis until day 20. At day 20 of arthritis all mice were sacrificed and serum TNF-α was measured using ELISA technique. Biopsies of the left knee joint were taken for histopathological evaluation.Results:The results indicate that G.glabra caused inhibition of histopathological features of antigen-induced arthritis in dose dependent manner. G.glabra also caused reduction of serum TNF-α concentration in antigen-induced arthritis model in dose dependent manner.Conclusions:Glycyrrhiza glabra can significantly inhibit antigen-induced arthritis in mice. This effect seems to be in dose dependent manner.Key words:Rheumatoid arthritis, G. glabra, TNF-α, antigen induced arthritis.


Article
EFFECT OF CHITOSAN AND DEXTRIN COMBINATION ON EXPERIMENTALLY-INDUCED THERMAL INJURY IN RABBITS

Authors: Noor A.M. Hassan نور احمد محمد حسن --- Abdulkareem H. Abd عبد الكريم حميد عبد --- Bahaa F. Hussein بهاء فخري حسين
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2016 Volume: 14 Issue: 2 Pages: 125-130
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Burn is a major health problem, life threatening with a high mortality and morbidity; Chitosan and its formulations are used as a topical dressing in wounds and burns management due to its nontoxic, hemostatic, healing stimulant, antimicrobial, biocompatible and biodegradable properties as well as its vehicle use to deliver biopharmaceuticals, antimicrobials and growth factors into tissue.Objective:To evaluate the effects of chitosan-dextrin combination on induced burn in rabbits.Method:Forty domestic male rabbits, weighing 1250-1750 kg were divided into five groups, each of eight animals: AH group: apparently healthy rabbits, BWT group: left with no treatment, AR group: treated with Aqua Rosea, AG-S group: treated with silver sulfadiazine cream and CH-D group: treated with chitosan – dextrin combination; all animals (except AH group) wereinduced burn and treatedtopically on burned area once daily for 28days. Tissue levels ofvascular endothelial cell growth factor (VEGF), tumor necrosis factor alpha (TNF-α) and skin histological examination.Results: Histopathological evaluation showed enhances inflammatory response, vascularization, granulation tissue formation, and collage deposition due to the appropriate regulation of TNF-α and VEGF.Conclusion:Topical use of chitosan – dextrin combinationshowed effective and enhance wound healing activities.Key words:Thermal injury, burn, chitosan, dextrin.

Keywords

Thermal injury --- burn --- chitosan --- dextrin.


Article
The protective effect of Trigonella foenum- graecum L. seeds extract in high fat diet-streptozotocin induced hyperglycemic mice
فعالية الحماية لمستخلص بذور ال Trigonella foenum- graecum L. في(غذاء عالي الدهون-streptozotocin) المحفزة على ارتفاع الكلوكوز في دم الفئران.

Loading...
Loading...
Abstract

Diabetes mellitus is a chronic metabolic disorder which affects carbohydrate, protein, and fat metabolism. associated with high morbidity and mortality. characterized by hyperglycemia together with biochemical alterations of glucose and lipid metabolism. Hyperglycemia and hyperlipidemia result in development of oxidative stress, systemic and local inflammation which were an critical factors for degradation of β-cells, insulin resistance and type II diabetes .Herbal medicine is one of the therapeutic strategies in the management of diabetes mellitus. It is considered to be less toxic and have fewer side effects than synthetic ones .The objective of this study was to investigate the possible protective effect of Trigonella foenum- graecum L. seeds(TFG) extract in High Fat Diet ∕ Streptozotocin -induced toxicity in mice. The seeds of TFG were defatted by soxhelt apparatus with n-hexane ,then marc was macerated with ethanol to obtain total ethanol extract. Thirty two male albino mice were fed high fat diet for two week plus single dose of streptozotocin 120mg ∕kg intraperitoneally to generate typeII diabetes model with increased adiposity. The protective and hypoglycemic activity of seeds extract against toxicity and hyperglycemia induced was evaluated in mice. Glimepiride was used as a standard treatment .FBG, serum C-peptide ,TC , Triglycerides ,HDL, LDL levels were measured at the end of 28th day of experiment then the animal sacrificed and liver and pancreas sections were prepared and stained for histological evaluation. This study was resulted in that (TFG) seed extract cause; FBG, serum lipid profile in hyperglycemic mice significantly decrease, It also increase serum C-peptide ,Glutathione and decrease Malondialdehyde levels significantly compared to untreated group; this was associated with improving histological features that impaired during STZ exposure. In conclusion, Defatted seeds extract has potential anti-hyperglycemic and hepatoprotective effect with free radical scavenging ability against STZ-induced toxicity.

داء السكري هو اضطراب مزمن في التمثيل الغذائي للكربوهيدرات والبروتينات والدهون ويكون مرتبطا باحتماليه عالية الخطورة التي تؤدي الى الموت المصاحبة لارتفاع مستوى الكلوكوز في الدم مع اختلال في التمثيل الغذائي للكلوكوز والشحوم و البروتينات ان ارتفاع مستوى الدهون والكلوكوز في الدم من العوامل المؤدية الى الاجهاد التأكسدي. يعد الالتهاب العام والموضعي من العوامل الأساسية التي تؤدي الى تحطم خلايا البنكرياس نوع البيتا و المقاومة لتأثير الأنسولين ومرض السكري من النوع الثاني .تعد الاعشاب الطبية تعد واحده من اهم الاستراتيجيات العلاجية لمرض السكري حيث انها تعتبر اقل سميه واقل اعراض جانبيه من العلاجات الصناعية وعليه صممت الدراسة لتقيم تأثير المحلول المائي الكحولي لمستخلص بذور الحلبة في حماية الكبد والبنكرياس من حالات التسمم بواسطة الستربتوزوتوسين مع غذاء عالي الدهون في الفئران. بعد ازالة الدهون من البذور بواسطة الهكسان عوملت مع الايثانول المائي بنسبة ثمانون بالمئه ثم بالتبخير والترشيح للحصول على المستخلص الذي اعطي للحيوانات. استخدمت اثنان وثلاثون من ذكور الفئران في الدراسة الحالية ,و قسمت الفئران الى أربعة مجاميع بواقع ثمانية حيوانات لكل مجموعة .اعطيت المجموعة الاولى غذاء عالي مع محلول داخل البريتون من الستريت بفر اما المجموعات الباقية فقد تم تحفيز ارتفاع نسبة الدهون بالدم و ذلك عن طريق اعطائهم غذاء غنيا بمادة الكوليسترول لمدة اسبوعين مع محلول داخل البريتون من الستريت بفر الستريبتوزوتوسين .وقيمت فعالية الحماية لتأثير انخفاض مستوى الكلوكوز بالدم في الفئران. "glimepride يستخدم كعلاج قياسي للمقارنة" بعد انتهاء مدة العلاج , جمعت عينات الدم للتحليل وعزل نسيج الكبد و البنكرياس لغرض الفحص النسيجي و كذلك لفحص العلامات الحيوية داخل النسيج. اظهر استخدام مستخلص بذور Trigonella foenum- graecum انخفاض واضح في العلامات الحيوية (MDA) في مصل الدم و العلامات النسيجية عند مقارنتها مع المجموعات التي لم تعالج ,وكذلك عند مقارنته مع بقية المجموعات. فيما يخص تأثيره على نسبة الدهون اظهرت النتائج انخفاضا ملحوظا في مستوى الدهون و الكلوكوز في الدم وارتفاعا ملحوظا في( GSH) ومستوى ال Cببتيد في مصل الدم. بالإضافة الى تماثل الخصائص النسيجية بعد فحصها الى الحالة الطبيعية خلال التعرض للستربتوزوتوسين . في النهاية تم الاستنتاج بان مستخلص بذور Trigonella foenum- graecum له افضل فعالية واضحة كخافض للكلوكوز في الدم و كمضاد الأكسدة المساهم في حماية الكبد والبنكرياس ضد التسمم الناتج بواسطة الستربتوزوتوسين وتم اثبات ذاك من خلال الفحص النسيجي .


Article
4.EFFECTS OF CIPROFLOXACIN ON MALE FERTILITY PARAMETERS AND SPERM DNA INTEGRITY IN RATS

Loading...
Loading...
Abstract

Background: The administration of antibiotics is of great value in the therapy of infections of male genital tract, which may have an effect on fertility. Antibiotics are generally prescribed for a variety of infections. A number of patients requiring assisted conception occasionally show evidence of reproductive tract infections. While fluoroquinolones being excessively prescribed in the treatment of male genital tract infections, too little information concerning their outcome on fertility are present.Objective: To estimate ciprofloxacin effects on sperm function parameters in addition to inspect whether ciprofloxacin can affect the integrity of sperm DNA.Methods:In the present study, 48 male adult rats were enrolled. The animals were randomly allocated into six groups; four ciprofloxacin treated groups, which were treated with either (40 mg/kg/day) or (80 mg/kg/day) of ciprofloxacin and 2 control groups. For each dose, the treatment maintained for either 14 days or 28 days. At the end of each duration of treatment, certain epididymal sperm function parameters: sperm morphological normality, sperm concentration and sperm motility were analyzed together with sperm DNA integrity analysis.Results: A significant reduction in sperm concentration, motility and percentage of morphologically normal sperm (in a dose dependent manner) was observed when ciprofloxacin administered for 28 days. The level of DNA fragmentation was significantly elevated with a significant reduction in sperm chromatin quality in ciprofloxacin treated groups whereas serum testosterone level was not significantly affected.Conclusion: Ciprofloxacin can adversely influence fertility parameters in male rat.Keywords: Fertility, Ciprofloxacin, Sperm DNACitation: Abd AH, Al-Dujaily SS, Al-Saray DA. Effects of ciprofloxacin on male fertility parameters and sperm DNA integrity in rats. Iraqi JMS. 2018; 16(4): 378-384. doi: 10.22578/IJMS.16.4.4

Keywords

Fertility --- Ciprofloxacin --- Sperm DNA

Listing 1 - 5 of 5
Sort by
Narrow your search

Resource type

article (5)


Language

English (5)


Year
From To Submit

2019 (1)

2018 (1)

2017 (1)

2016 (1)

2015 (1)