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Isolation and Diagnosis of Phenolic Compounds in Pomegranate Peel and Their Use in Inhibition of Intestinal Pathogenic Bacteria Isolated from Human Intestine and Stomach

Author: Afraa Abdul‑Wahab Ali, Ali Mohammed Jawad, Mufeed Jalil Ewadh
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 1 Pages: 1-4
Publisher: Babylon University جامعة بابل


Background: Pomegranate (Punica granatum L.) has gained commercial importance in food and health industries due to increasingscientific evidence linking its consumption to better health outcomes. Objective: This study aimed to detect the active substances(phenols, tannins, flavonoids, alkaloids, and saponins) in the pomegranate peel and their effectiveness against bacteria isolated fromintestine and stomach which included Salmonella and Escherichia coli, which are responsible for most gastrointestinal diseases.Materials and Methods: The experiment was designed randomly and was statistically analyzed using the least significant differenceat P < 0.05. The plant extracts were obtained by alcoholic extraction using Soxholet. The compounds were diagnosed qualitativelyand quantitatively using reference methods. Results: The results showed that peels contained high concentration compounds ofalkaloid, tannic acid, and saponins. A 15% alcohol extract gave a high inhibition rate compared to the water extract and alcohol at arate of 40 mm corresponds to 19 mm in the chloroform extract and 20 mm in the water extract at the concentration of 15%. A 15%concentration of alcoholic extract with antacid ampicillin and chlorophyll was compared with high efficacy compared to effectiveanti-ampicillin.

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