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Article
ASSOCIATION OF PORPHYROMONUS GINGIVALIS WITH RHEUMATOID ARTHRITIS

Authors: Sadeq k. Hachim صادق كاظم حاجم --- Ahmed A. Abbas احمد عبد الحسن عباس --- Mohammed H. Alosami محمد هادي العصامي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2016 Volume: 14 Issue: 3 Pages: 231-236
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Rheumatoid arthritis (RA) is chronic systemic inflammatory disease. Porphyromonus gingivalis (P. gingivalis) produce peptidyle arginine enzymes, which lead to citrullination of human protein then lead to formation anticitrullinated peptide antibody (ACPA).Objective:To investigate the role of P. gingivalis as environmental factor for RA and association of P. gingivalis with development of ACPA.Methods:This study included 31 newly diagnosed RA patients with periodontitis, which included 22 females and 9 men in addition to 30 individual as healthy controls, which included 20 females and 10 men. The exclusion criteria included autoimmune disease (systemic lupus erythematosus, Bachet disease, ankylosing spondylitis, multiple sclerosis), attending Department of Rheumatology in Baghdad Teaching Hospital during period from May 2014 to January 2015. The age range was 20 to 68 years. Disease activity score 28 (DAS28) was calculated for each patient. Five ml of blood sample was taken for detection of ACPA antibody while gingival cervicular fluid was taken by paper point for detection of P. gingivalis by polymerase chain reaction (PCR) with specific primer for fimbrial antigen (fimA).Results:The frequency of positive cases with P. gingivalis were 13/31(41.90%) while in healthy controls was zero with significant P value (<0.001). The association between anti cyclic citrullinated peptide (ACCP) antibody and frequency of positive cases for P. gingivalis was significant (P<0.041). The association of P. gingivalis positivity and DAS28 was non-significant (P= 0.003).Conclusions:P. gingivalis showed positive association with RA in newly diagnosed patients. The frequency of positive cases for P. gingivalis revealed association with positivity of anti-CCP.Key words:RA, ACCP, P. gingivalis

Keywords

RA --- ACCP --- P. gingivalis


Article
THE EFFECT OF TUMOR NECROTIC FACTOR ALPHA POLYMORPHISM ON RESPONSE TO BIOLOGICAL TREATMENT FOR RHEUMATOID ARTHRITIS PATIENTS

Authors: Sadeq k. Hachim صادق كاظم حاجم --- Ahmed A. Abbas احمد عبد الحسن عباس --- , Mohammed H. Alosami محمد هادي العصامي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 3 Pages: 220-226
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: The tumor necrotic factor alpha (TNF-α) was associated with rheumatoid arthritis (RA) pathogenesis and inflammation of joint, for this consider important target for drugs (TNF inhibitors) as for example infliximab.Objective: To investigate the role of TNF-α-308 polymorphism G>A in unresponsiveness to biological treatment (infliximab) in RA patients.Methods: This study included 29 RA patients treated with infliximab attending Department of Rheumatology in Baghdad Teaching Hospital and diagnosed according to American College of Rheumatology (ACR) with 30 persons as healthy controls. The age range was 20 to 68 years. The DAS28 was calculated for each RA patients. Blood samples were taken from them during the period from May 2014 to January 2015. The blood samples were tested by polymerase chain reaction (PCR) for TNF-α-308 G>A then by restriction fragment length polymorphism (RFLP) for A allele and G allele. HLA-DR genotyping by PCR-SSO.Results: The frequency of A allele was 23 (39.7%) in RA patients while G allele was 35 (60.3%) in RA patients, whereas in in controls A and G alleles frequencies were 5 (8.3%) and 55 (91.7%) respectively. The A allele was associated with high DAS28 19 (82.6% while for G allele was 1 (2.9%) and DAS28 showing significant association with TNF-α-308 polymorphism G>A. The distribution of A allele in female RA patients was 16 (36%) whereas G allele was 28(64%), while in male RA patients, A and G allele were 7 (50%) for each of them. The HLA-DRB1 not showing significant association with TNF-α-308 polymorphism G>A.Conclusion: The TNF-α-308 polymorphism G>A has effect on response to biological treatment (infliximab). The A allele of TNF-α was associated with unresponsiveness to infliximab and RA patients who carry this allele have high DAS28 score. The HLA-DRB1 not showing association with TNF-α-308 polymorphism G>A.Keywords: RA, TNF-α polymorphism, infliximab, HLA-DR.Citation: Hachim Sk, Abbas AA, Alosami MH. The Effect of tumor necrotic factor alpha polymorphism on response to biological treatment for rheumatoid arthritis patients. Iraqi JMS. 2017; Vol. 15(3): 220-226. doi: 10.22578/IJMS.15.3.2

Keywords

RA --- TNF-α polymorphism --- infliximab --- HLA-DR


Article
DETECTION OF HEPATITIS C VIRUS IN IRAQI PATIENTS WITH ORAL LICHEN PLANUS

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Abstract

Background:Oral Lichen planus (OLP) is chronic mucocutaneous disorder with or without involvement skin, of unknown etiology. Lichen planus (LP) is T-cell-mediated chronic inflammatory disease of the stratified squamous epithelium. It can affect oral mucosa, skin, esophagus, nasal mucosa, larynx, genitalia, urinary tract, hair follicles and the eyes. Latterly has been focused on presence association between OLP and hepatitis C virus (HCV) infection, but this relation is not stable, since the prevalence of this virus in patients varies depended on studies, ranging from 0% to 67.8%. Objective:This study was established to investigate the relationship between the OLP and HCV infection in Iraq and to detect the virus by Real-time polymerase chain reaction (RT-PCR).Methods:Blood samples were collected from 62 patients with OLP and controls to detect the HCV in those patients by using one step anti-HCV test, ELISA test and RT-PCR.Results:The current study revealed that the early detection of HCV by one step Anti-HCV for all OLP patients was negative and by ELISA showed that four patients (12.5%) of OLP were positive for HCV. While using RT-PCR found that only one patient (3.1%) was positive for the presence of the virus.Conclusion:There was not relationship found between OLP and HCV infection in Iraqi patients.Keywords:Oral lichen planus, HCV, RT-PCR, ELISA

Keywords

Oral lichen planus --- HCV --- RT-PCR --- ELISA


Article
4.SERUM LEVELS OF GLYCODELIN A AND SOLUBLE INTRACELLULAR ADHESION MOLECULE-1 AS BIOMARKERS FOR ENDOMETRIOSIS

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Abstract

Background:Endometriosis is a benign chronic disease, characterized by the presence and proliferation of functional endometrial gland and stroma outside the uterine cavity.Objective: To evaluate the usefulness of intracellular adhesion molecule-1 (ICAM-1) and Glycodelin (Gd) as a biomarker for the diagnosis of endometriosis and to help in detection of various stages of endometriosis.Methods: Forty-four patients with endometriosis and 35 apparently healthy women as control were enrolled in this study from November 2015 to April 2016. All individuals were subjected to blood sampling for measuring their serum ICAM-1 and Gd A level by using enzyme linked immune sorbent assay technique. Results: The current study revealed significantly higher serum levels of ICAM-1 and Gd in patients group in comparison with healthy control. In addition, the sensitivity and specificity for ICAM-1 and Gd A in serum were 61%/ 66%, 76%/ 85% respectively.Conclusion: The ICAM-1 and Gd A level in serum may be useful as noninvasive test for diagnosis of endometriosis in all stages.Keywords: Endometriosis, ICAM-1, GlycodelinCitation: Draj HA, Abbas AA, Abdullah TH. Serum levels of Glycodelin A and Soluble Intracellular Adhesion Molecule-1 as biomarkers for endometriosis. Iraqi JMS. 2017; Vol. 15(4): 345-349. doi: 10.22578/IJMS.15.4.4

Keywords

Endometriosis --- ICAM-1 --- Glycodelin


Article
8.INTERLEUKIN-4 SINGLE NUCLEOTIDE POLYMORPHISM C-590T POLYMORPHISMS IN RELATION TO ASTHMA

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Abstract

Background:Single nucleotide polymorphisms in the promoter regions of genes encoding for some interleukins may associate with occurrence of asthma.Objective:To investigate the association of single nucleotide polymorphisms of interleukin-4 (IL-4) (C-590T) and asthma.Methods:Forty-five patients with asthma and 40 apparently healthy subjects (represent the control group) were enrolled in this study. Blood samples were collected from both patients and controls. DNA was extracted from blood samples and gene fragments corresponding to IL-4 C-590T were amplified with specific primers using conventional PCR technique. Results:The heterozygote genotypes of IL-4 C-590T (CT) showed significant association with asthma (OR = 3.922, 95% CI= 1.153-13.339, P = 0.028). Conclusion:These results suggest the significance of IL-4 C-590T polymorphism as a risk factor for asthma.Keywords:Asthma, interleukin-4, polymorphismCitation:Gaidan, Abbas AA, Hassan MA, Hashim HM. Interleukin-4 single nucleotide polymorphism C-590T polymorphisms in relation to asthma. Iraqi JMS. 2018; Vol. 16(1): 51-56. doi: 10.22578/IJMS.16.1.8

Keywords

Asthma --- interleukin-4 --- polymorphism

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