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Article
The Role of β2 Antagonist (Timolol) and β2 Agonist (Salbutamol) on Cell Migration in vitro.

Authors: Mahmood Jasem Jawad --- Ahmed Rahma Abu-Raghif --- Hamed Naji Obied
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 4 Pages: 694 -700
Publisher: Babylon University جامعة بابل

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Abstract

Cell migration is a complex and dynamic biological process, it's the movement of the cell from one area to another, generally in response to a chemical signal. It is an essential feature of living cells for functions such as wound repair, tissue regeneration, cell differentiation, and immune response. Keratinocytes have the enzymatic machinery to generate catecholamines, they can synthesize endogenous epinephrine, which could be locally secreted into the wound and function in an autocrine manner. To determine whether beta2-adrenergic receptor (β2AR) antagonist and agonist altered endothelial cells (EC) migration, single-cell migration (SCM) assays were performed with human umbilical vein endothelial cells (HUVEC) in the presence and absence of β2AR agonist and antagonist. Screening the tested drugs revealed that the salbutamol (β2AR agonist) significantly decreases migration rate on (HUVEC) compared with control group, while timolol (β2AR antagonist) significantly increases the migration rate. In conclusion the administration of timolol increase single cell migration resulting and enhancement of re-epitheliazation, while administration of salbutamol decrease single cell migration resulting in inhibition of wound re-epitheliazation.

Keywords

Migration --- salbutamol --- timolol --- HUVEC --- In vitro


Article
Antibacterial activity of Trigonella Foenum- groecum essential oil against skin infection with Staphylococcus aureus: In vitro and in vivo studies

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Abstract

ABSTRACTBackground: Multi- drug resistant (MDR) Staphylococcus aureus infections have become a major public health concern in both hospital and community settings.Objective: to investigate the antibacterial activity of T. Foenum- groecum essential oil against skin infection with S. aureus and to study probable synergistic activity in combination with Clindamycin.Type of the study: Cross-sectional study.Methods:Antibacterial activity of T. Foenum- groecum essential oil extract (1.2gm/100 µl) was investigated in multi- drug resistance (MDR) Staphylococcus aureus specimen isolated from patients with skin infection in Baghdad. T. Foenum- groecum use externally for cellulites and skin inflammation due to the presence of diosgenin .fast liquid chromatography was used to separate these components. Results: Antibiotics combinations revealed that T. Foenum- groecum essential oil with Clindamycin against MDR isolates of S. aureus showed a synergistic effect when used as 1/4 MIC for each antimicrobial. In vivo study was executed to determine antibacterial activity of these compounds by induction of skin infection with Staphylococcus aureus in mice and the treatment begun after 4hrs later and continue to seven days then skin biopsy was taken and sent for histopathological examination. Conclusions: According to the results of this study, we can conclude that T. Foenum- groecum essential oil has remarkable antistaphylococcal activity. Combination of T. Foenum-groecum essential oil with Clindamycin was more effective than Clindamycin alone in treatment of skin infection with Staphylococcus aureus.

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