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Article
Molecular detection of invA gene for Salmonella spp. isolates from poultry in Babil province-Iraq

Authors: Alaa Abdel-Kadhim Jawad --- Nafea Sabeeh Jasim --- Safaa Jabbar Hamzah
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 1 Pages: 60-63
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

A total of 150 poultry samples (age from 1 to 49 day) were collected from different locations at Babylon province ) Al-hashimiya , Al-madhatiya and Al-Qasim ) from November 2015 to April 2016. These samples were collected from different part of the body (Liver tissue, Yolk sac content, and cecal swab). Salmonella spp. was isolated and identified using bacterial culturing on selective media, in addition to, biochemical and Mini API 20E and serotyping by monovalent antisera. Polymerase chain reaction (PCR) was used to detect invA of Salmonella spp. The results revealed that the rate of Salmonella isolates from poultry specimens were (11) 7.3% using cultural and biochemical tests, the results of serotyping revealed these isolates belong to Salmonella spp. The PCR technique was used to detect invA gene, these Salmonella isolates appeared to contain this gene since DNA amplification showed one distinct band (size 389 bp) when electrophoresed on agarose gel. The results of this study revealed that the PCR technique had a high specific in detection of Salmonella spp. When compared with other conventional detection methods

Keywords

Poultry --- Salmonella --- PCR --- invA gene


Article
Molecular survey and phylogeny of Anaplasma Ovis in small ruminants in Al-Qadisiyah Province, Iraq

Authors: Yahia I. Khudhair --- Hayder N. Ayyez --- Alaa Abdel-Kadhim Jawad
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 137-142
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Anaplasma ovis is a one of an important group of the tick-borne pathogen and an obligate intraerythrocytic bacterium, which infects sheep and goats as well as wild ruminants. The phylogenetic study of A. ovis in small ruminants has not studied yet in Iraq. In this study, the presence of A. ovis was investigated in a total of 80 (40 sheep and 40 goats) obtained from 16 randomly selected small ruminants flocks in AL-Qadisiyah in Iraq. All blood samples tested microscopically, firstly by Diff-quick stained blood smear for the detection of intraerythrocytic pathogens. Total DNA was extracted from a sample and submitted to PCR based on fragment amplified of 16S rRNA gene followed nucleotide sequencing and phylogenetic analysis. Six out of 80 samples, (10%) from sheep and (5%) from goats gave positive results. The results of nucleotides sequencing and multiple alignments revealed related Iraqi isolates had a high identity (99.70% - 97.21%) with isolates of other countries, the phylogenetic analysis demonstrated that Iraqi isolates of A. ovis fell one clade near to Russian and Sweden strains and shared 99.7 %-98.36 % with them. In conclusions: this work indicates to detect A. ovis at a low rate in sheep and goat in Iraq and it had a high genetic similarity to world strains.

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Article
Detection of rfbj(B) gene of Salmonella serogroup-B isolated from patients with salmonellosis

Authors: Aqeel Reheem Hassan --- Hamad Al-Hamadani --- Alaa Abdel-Kadhim Jawad , Adnan Hamad Al-Hamadani, Aqeel Reheem Hassan
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2012 Volume: 5 no 2 Issue: 12 Pages: 1413-1419
Publisher: Kerbala University جامعة كربلاء

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Abstract

summary: A total of 480 fecal samples were collected from patients (less than 18 years old), of both sex suffering from diarrhea who admitted to Al-Diwaniya Teaching Hospital and the Teaching Hospital of Maternity and Pediatrics in Al- Qadisiya governorate. Salmonella spp. were isolated and identified using bacterial culturing on selective media, in addition to, biochemical and serotyping by monovalent antisera. Polymerase Chain Reaction (PCR) was used to detect rfbj(B)gene encoding for biosynthesis of LPS of group B Salmonella. The results revealed that the rate of Salmonella isolates in fecal samples of patients were 7.9% using cultural and biochemical methods .The result of Salmonella isolates by serotyping using monovalent antisera revealed that 30 out of 34 isolates (88 %) belong to S. Typhimurium serotype , while the remain belong to S. Enteritidis ( 2 isolates ) and S.Meunchen (2isolates). when the PCR technique was used to detect the presence of rfbj(B) gene, 34 Salmonella isolates belong to Salmonella serogroup (B) appeared to contain this gene since DNA amplification showed one distinct band (882 bp) when electrophorised on agarose gel. The results of this study revealed that the PCR technique had a high specifity (100%) in detection of Salmonella serogroup B in comparison to cultural, biochemical and serological tests.

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Article
The use of fimA gene primers for detection of Salmonella spp. isolated from children with diarrhea

Authors: Nasma N. Al-Hajia نسمة ناجي الحجية --- Alaa Abdel-Kadhim Jawad علاء عبد الكاظم --- Adnan Hamad Al-Hamadani عدنان حمد الحمداني
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2010 Volume: 6 Issue: 9 Pages: 223-234
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

A total of 480 fecal samples were collected from children (less than 3 years old) , of both sexes suffering from diarrhea who admitted to The Teaching Hospital of Maternity and Pediatrics in Al- Diwaniya governorate. Salmonella spp. were isolated and identified using bacterial culturing on selective media, in addition to, biochemical and Mini API 20E. Polymerase chain reaction (PCR) was used to detect fimA gene encoding for biosynthesis of fimA of Salmonella spp. The results revealed that the rate of Salmonella isolates in fecal samples of patients were (38/480) 7.9% using cultural and MiniAPI20 E, when the PCR technique was used to detect the presence of fimA gene, 34 Salmonella isolates belong to Salmonella spp. appeared to contain this gene since DNA amplification showed one distinct band (size 85 bp) when electrophorised on agarose gel. The results of this study revealed that the PCR technique had a high specifity (100%) in detection of Salmonella spp. in comparison to cultural and biochemical and Mini API20E tests.

جمعت 480 عينة براز من الأطفال دون سن ثلاث سنوات من الذكور والإناث الراقدين والمراجعين إلى مستشفى النسائية والأطفال التعليمي الذين كانوا يعانون من الإسهال.أجريت الدراسة للمدة من تشرين الثاني 2008 ولغاية شهر تشرين الأول 2009 . تم عزل بكتريا السالمونيلا من خلال الزرع البكتيري للعينات على أوساط زرعيه أغنائيه و انتقائية وشخصت من خلال الاختبارات الكيميوحيوية واستعمل Mini API 20E و استعملت تقنية التفاعل التضاعفي لسلسلة الدنا ( (PCR للكشف عن وجود الجين (fimA) المشفر للأهداب الخاصة بجنس السالمونيلا Salmonella spp. .أظهرت النتائج إن نسبة عزل بكتريا السالمونيلا Salmonella spp. من عينات براز الأطفال كانت (/48038 (7.9% ، إذ وجدت فروق معنوية عند مستوى احتمالية (P<0.01) في نسبة العزل عند استخدام الطريقة المظهرية التقليدية والجينية (PCR) . أظهرت تقنية الـPCR ألمفرده للكشف عن الجين المشفر لتخليق الهدب ( (fimA ان جميع عزلات السالمونيلا قيد الدراسة تمتلك هذا الجين فقد تظهرت حزمة واحدة ناتجة من عملية التضخيم للـDNA وكان حجمها 85 زوج قاعدي على هلام الاكاروز. كشفت نتائج هذه الدراسة ان تقنية الـPCR أظهرت نوعية (Specificity) عالية (100%) في الكشف عن السالمونيلا (. (Salmonella spp. مقارنة بالفحوصات الأخرى الزرعية والكيموحيوية, فحص Mini API 20E.

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Article
Outer membrane protein C (ompC) gene as target for diagnosis of Salmonella spp. using polymerase chain (PCR) reaction
مورث بروتين الغشاء الخارجي (ج) هدف لتشخيص جنس السالمونيلا باستخدام تفاعل البلمرة

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Abstract

A total of 1200 different specimens collected from human and animals sources. 600 specimens from patients suffering from diarrhea who were admitted to Al-Diwaniya Teaching Hospital for Maternity and Children. 600 specimens from gall bladder (bile) of cattle from Al-Diwaniya slaughter house. This study was conducted during the period that extended from May 2013 to April 2014. Salmonella spp. were isolated and identified using bacterial culturing on selective media, biochemical, API 20E, serotyping by polyvalent and conformation by PCR. Polymerase chain reaction (PCR) was used to detect ompC gene encoding for biosynthesis of outer membrane protein C of Salmonella genus. The results revealed that the rate of Salmonella isolates was 0.5% (3/600) from human and 1% (6/600) from animals. The PCR technique revealed that 9 isolates of Salmonella spp. were contain ompC gene (DNA amplification showed one distinct band with molecular weight of 204 bp when electrophorised on agarose gel).The results of this study revealed that the PCR technique had a high specificity in detection of Salmonella spp., in comparison to culture and biochemical test, Mini API 20 E and serological tests. The present study found no significant differences between human and animal isolates.

جمعت 1200 عينة مختلفة من الانسان والحيوان، منها 600 عينه تم جمعها من الاطفال المرضى الراقدين والمراجعين إلى مستشفى النسائية والأطفال التعليمي واللذين يعانون من الإسهال و600 عينه مراره ماشيه من مجزرة لحوم الديوانية. أجريت الدراسة للمدة من شهر ايار 2013 ولغاية شهر نيسان 2014. تم عزل بكتريا السالمونيلا من خلال الزرع البكتيري للعينات على أوساط زرعيه أغنائية وانتقائية وشخصت من خلال الاختبارات الكيميوحيوية واستعمل Mini API 20E و كما تم استخدام اختبار التلازن باستعمال المصول المضادة متعددة التكافؤ. استعملت تقنية التفاعل التضاعف لسلسلة الدنا ((PCR للكشف عن وجود الجين (ompC) المشفر لبروتين الغشاء الخارجي نوع C الخاصة بجنس السالمونيلا Salmonella spp.. اظهرت النتائج ان نسبة عزل بكتريا السالمونيلاSalmonella spp. من عينات براز الأطفال كانت (600/3(0.5% ، وكانت نسبة العزل من الحيوان (600/1(1% إذ وجدت فروق معنوية عند مستوى احتمالية (P<0.01) في نسبة العزل عند استخدام الطريقة المظهرية التقليدية والجينية (PCR). واظهرت نتائج استعمال Mini API 20E و نتائج استخدام اختبار التلازن باستعمال المصول المضادة متعددة التكافؤ. وتقنية التفاعل التضاعفي لسلسلة الدنا ((PCR للكشف بان هنالك 9 عزلات للسالمونيلا .أظهرت تقنية الـPCR المفردة للكشف عن الجين المشفر لتصنيع الهدب ((ompC ان جميع عزلات السالمونيلا تمتلك هذا الجين فقد تظهرت حزمة واحدة ناتجة من عملية التضخيم للـDNA وكان حجمها 204 زوج قاعدي على هلام الاكاروز. أظهرت نتائج هذه الدراسة إن تقنية الPCR نوعية عالية مقارنة بالفحوصات الأخرى الزرعية والكيموحيوية والفحوصات المصلية وكذلك Mini API 20E.

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