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Article
Bacteriological Study for Detection The Role of Fructan Produced from Pseudomonas putida as Enhancer for Lactobacillus acidophilus Growth

Author: Alaa Raheem Kazim
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 21 Issue: 1 Pages: 98-103
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Fructan is a kind of biopolymer produced from plants, fungi, yeast and bacteria. It is classified as prebiotics food and enhanced the growth of probiotics bacterial strains which improves the health of the individual. This article aimed to study the production and extraction of fructan from Pseudomonas putida isolated from food samples and study the effect of fructan on the viable cell number of Lactobacillus acidophilus. The results showed that Pseudomonas putida have the ability to produce fructan when cultured on production medium (YPS), fructan exhibited as white powder when extracted from YPS and the amount of fructan was 3.6 mg/100 ml, also the results showed that fructan can increase the viable cell number of Lactobacillus acidophilus when added to MRS. FTIR analysis of fructan produced from Pseudomonas putida revealed that the functional groups found in chemical structure of fructan, which it was (C-O, C-H, O-H and C=O).


Article
Increasing Cellulose Production from Rhizobium leguminosarum bv. viciae

Authors: Suaad Ali Ahmed --- Alaa Raheem Kazim --- Hussam Mahmood Hassan
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2017 Volume: 20 Issue: 1 Pages: 120-125
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Bacterial cellulose (BC) is a type of biopolymer produced as primary metabolism product by many genera of bacteria such as Acetobacter, Rhizobium, Agrobacterium, Psuedomonas and Sarcina. This article aimed to study the effect of some factors on cellulose production from Rhizobium leguminosarum bv. viciae which isolated from root nodules of Vicia faba plant collected from Abu-Ghraib in Baghdad. Some environmental and nutritional factors were examined including : carbon and nitrogen sources, pH, temperature and incubation period. Results showed that maximum production of cellulose was gained by Hestrin-Schram medium (HS medium) containing 2% fructose as carbon source, 1% yeast extract as nitrogen source, as well as optimum pH, temperature, and incubation period were (5, 30°C for 7 days) respectively .Cellulose dry weight was analyzed by Fourier transform infrared spectroscopy (FTIR) technique and the results showed the presence of the functional groups of bacterial cellulose. [DOI: 10.22401/JNUS.20.1.17]

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