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This study was aimed to evaluate the role of the Pseudomonas fluorescensand Aqueous extract of Cocklebur leaves in the control of the laboratoryFusarium oxysporum have been. Two isolates of P.fluorescens were isolatedfrom Root of Cucumber Seedlings. The first one named Pseudomonasfluorescens (SH1) and the second named Pseudomonas fluorescens (SH2).The Results showed that the higher Inhibition among isolates of P.fluorescens for F. oxysporum was (100%) compared with the control treatment(0%). Also the of the experiment showed that the aqueous extract of theCocklebur leaves has an inhibition effect on the growth of mycelium pathogen F.oxysporum on the PDA media according to the concentrations used, where thepercentage of inhibition reached to 56, 66.67, 69.50 and 100%, with theconcentrations of 10, 20, 30 and 40% respectively, compared to the controltreatment which has 0% inhibition. In addition results showed that the aqueousextract of the Cocklebur leaves does not have inhibition of on the P.fluorescens(SH1) and P.fluorescens (SH2) by discs method where does not show anyinhibition zone around the discs also in tablets soaked in sterile distilled wateronly.

extract --- Cocklebur plant --- Pseudomonas fluorescens --- Fusarium oxysporum --- Aqueous extract