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THE PROLIFERATIVE PATTERN OF THE VENTRICULAR NEURO-EPITHELIUM OF EMBRYONIC RAT FOURTH VENTRICLE

Author: Ali HA Al-Anbaki علي حسين العنبكي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2012 Volume: 10 Issue: 2 Pages: 128 -133
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Studies have shown that the ventricular neuroepithelium (VN) of the dorsal metencephalonrepresents the site of the cerebellar GABAergic neurons progenitors' production. These studies used different histological techniques but all have provided qualitative information regarding the biosynthesis and cell mitosis.Objective: Quantifying the proliferative activity of the cells at the (VN) during the embryonic period.Methods: Nine age groups from day 13 to day 21 albino rat embryos Rattusrattusnorvegicusalbinus were investigated with Ag-NOR staining technique.Results:There was a statistically significant difference (p<0.01) between cellular activity at different age groups with a maximum proliferative activity on the embryonic day 15.Conclusion: Ag-NOR staining technique provided a valuable quantitative index of cell proliferation at the (VN) of the developing cerebellum.Key words: Ventricular Neuroepithelium, Rhombencephalon, Ag-NOR, Quantitative


Article
THE PROLIFERATIVE PROFILE OF THE RHOMBENCEPHALICDEMILUNE IN THE DEVELOPING RAT CEREBELLUM: A QUANTITATIVE HISTOCHEMICALSTUDY

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Abstract

BackgroundStudies have shown that the cerebellum develops within the dorsal metencephalon creating a rhombencephalicdemilune (RD) which represents the formation site of the cerebellum granular cells progenitors. These studies used different histological techniques but all have provided qualitative information regarding the biosynthesis and cell mitosis at the RD.ObjectiveQuantifying the proliferative activity of the cellsat the RD during the embryonic period.MethodsSix age groups from day 16 to day 21 albino rat embryos Rattusrattusnorvegicus were investigated with Ag-NOR staining technique to quantify cell proliferation.ResultsThere wasa statistically significant difference (p<0.01) between cellular activity at different age groups with a surge during embryonic day 18.ConclusionsCorrelation with other studies revealed that Ag-NOR staining technique, which reflects protein biosynthesis and nuclear mitotic activity, provided a valuable quantitative measure of cellular proliferation in the developing rat cerebellum.Keywords Rhombencephalicdemilune, Rat, Developing Cerebellum, Ag-NOR, Quantitative, Histochemistry


Article
SEMI-AUTOMATED COMPUTATIONAL METHOD FOR SKELETAL MUSCLE FIBER TYPING WITH LECTINS: CORRELATION WITH MORPHOMETRIC STUDIES

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Abstract

Background :Muscle fiber typing has been an extensive field of study for many years. Though, limited researches applied lectin histochemistry in the clinical diagnosis of muscle disorders; attention was directed mainly towards enzyme histochemistry.Objective:The use of lectins as recognition systems based on specific protein-carbohydrate interactions in correlation with muscle fibers morphometric standards and optical density features to favor the diagnostic procedures of muscle disorders.Methods:Cross-sections of tibialis anterior muscle from 15 adult rats were stained with Con A, PNA, SBA, WGA, SWGA, LFA, UEA-I, and UEA-II lectins. Photographs of stained sections were analyzed with ImageJ 1.44 software for muscle fiber area, perimeter, optical density, and integrated density.Results:There were statistically significant differences between the parameters of muscle fiber types under study (P<0.05) concerning Con A, LFA and UEA-II lectins, but not for the remaining lectins, regarding the optical density and integrated density of muscle fibers.Conclusions:Lectins make accurate recognition of muscle fiber types on fixed paraffin sections when combined with computerized methods to quantify the features seen in muscle biopsies destined for pathological investigations.Key words:Lectins, Muscle fiber typing, Quantitative, Optical density, Morphometry

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