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ACTIVATION OF BACTERIAL CYTOCHROME P450 IN THE INTESTINE OF LARVAL MOSQUITOES AN INDICATION OF ITS ROLE IN THE INSECTICIDE RESISTANCE

Author: Ali Hassan Daghir Janabi
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2017 Volume: 16 Issue: 2 Pages: 85-91
Publisher: Basrah University جامعة البصرة

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Abstract

Mosquitoes apply very important impact on people life, and their control, day by day, isgetting harder and harder. This difficulty comes from different issues such as insecticideresistance. The gut microbiome (GM) in different animals has been discovered to add principleinfluence on the gastrointestinal tract physiology. For example, GM participates in degradationof some poisonus chemicals into safer molecules that could be handled by the animal host.Eukaryotic cytochrome P450 has been detected to inseret some degradation processes in otheranimal models such as zebra fish. However, these effects are not yet known in mosquitos. Inorder to stand on these problems and find if the prokaryotic P450 is involved, this study had beendone to find out if the GM has any role in developing such resistance in mosquitos. Larvae wereused in this experiment which exposed to benzothiozole (BZT) to trigger the hypothetical role ofprokaryotic P450. By using polymerase chain reaction (PCR), the result indicates that thebacterial P450 was initiated and might be involved in the BZT exposure handling


Article
Detection the presence of Bdellovibrio bacteriovorus in the bacterial DNA extracts of Culex pipiens intestine using polymerase chain reaction

Author: Ali Hassan Daghir Janabi
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 18-21
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Mosquitoes are tiny creatures but could do a lot of damages to the people life and especially by transmitting dangerous diseases such as dengue fever transported by Aedes aegypti. The control of these bugs is hostorically challenging and difficult. This difficulty increases after the emerging problems of insecticide resistance. The gut flora(GF) in different animals has been detected to place an important role on the physiology of the intestine. This study was conducted to discover if the Bdellovibrio bacteriovorus is a member of the GF of the mosquitoes. B. bacteriovorus is a bacterium that has been found in the intestine of animals and healthy and patient humans but not in mosquitoes. Seventy adult mosquitos of Culex pipiens were used for extraction of the bacterial DNA from their intestine. The 70 mosquitoes were assigned randomly to 7 groups, 10 mosquitoes for each group to pool the amount of DNA extracted. Each mosquito was dissected under the microscope to isolate the intestine and use it later for DNA extraction. After the DNA had been extracted using a phenol/chloroform method, I subjected the extracted DNA to polymerase chain reaction (PCR) to detect the presence of this bacterium among the bacteria of the intestine using the following specific primers: Bd529F (5’-GGTAAGACGAGGGATCCT-3’) and Bd1007R (5’-TCTTCCAGTACATGTCAAG-3’) that amplify a 481-bp of the 16S rRNA gene. By the amplification that happened in all 7 groups, the result indicates the presence of Bdellovibrio bacteriovorus in the intestine of the Culex pipiens. Because this bacterium preys on Gram-negative bacteria, our results help to use this bacterium to fight insecticide resistance that caused by the degradation of these chemicals by gram-negative bacteria in the ingestion of these mosquitoes.

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