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Article
SEROLOGICAL STUDY FOR DETECTION OF NEWCASTLE DISEASE VIRUS IN JAPANESE QUAILS IN SOME STATE OF DIYALA PROVINCE, IRAQ

Authors: Karim Sadun AL-Ajeeli --- Amer AL-Azawy
Journal: Diyala Journal of Agricultural Sciences مجلة ديالى للعلوم الزراعية ISSN: 20739524 Year: 2016 Volume: 8 Issue: 1 Pages: 13-21
Publisher: Diyala University جامعة ديالى

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Abstract

Newcastle disease (ND) is a highly contagious viral disease all over the world that could cause several losses for the poultry industry. The current study was conducted to clarify the real evidence that the quails play important role in the epidemiology source of NDV. Two groups of quail were used at 50 and150 day old for identification of the NDV. Antigen Rapid Test Kit (Bionote, South Korea) used to identify the positive cases, all positive cases by this kit were tested by Enzyme- Linked Immunosorbent Assay (ELISA) to determine the antibody titers against NDV in all study groups. The results of this study revealed that antibodies against NDV were detected with the mean titer of 2904.39 ±119.238 and 4664.36 ±136.659 to each age groups respectively, in spite of no clinical signs of NDV were observed in thetwo groups. The results referred that quail were carrier for the NDV and may be transmission the pathogen virus to other poultry that housed together, and that calls the attention to the importance of the quail from the epidemiological point of view as a potential source of NDV to commercial poultry (broiler or layer) that housed with or near quails.

Keywords

Newcastle disease --- Quail --- ELISA --- Diyala


Article
Identification and Differentiation of Mycobacterium Avium Subspecies Paratuberculosis Isolates Using and pAM-3 as a DNA Probe

Authors: Karim S.A. Al-Ajeeli --- Amer Al-Azawy --- Suri-Arshad --- Sharifah, S.H.
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2015 Volume: 8 Issue: 1 Pages: 29-37
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Mycobacteriu avium subsp.paratuberculosis (MAP) is the causative agent of Johne’s disease. It is a very slow growing bacterium on synthetic medium. The use of conventional methods for diagnosis is time consuming and not accurate.Objectives: The use of molecular biological techniques for fast and accurate diagnosis of Mycobacterium avium subsp.paratuberculosis isolates.Materials and methods: DNA was extracted and prepared from four Mycobacterium avium subsp.paratuberculosis and four Mycobacterium tuberculosis bovis isolates. The extracted DNA was subjected to PCR by using specific primers and the isolates were distinguished and differentiated by hybridization technique using pAM-3 specific probe developed in New Zealand.Results: The PCR products gave the requested DNA fragment of 163 base pairs. The probe reacted specifically with targeted DNA fragment of paratubreculosis bacterial isolates but not with tuberculosis bovis isolates.Conclusion: The use of PCR and specific DNA probe for the diagnosis of MAP is a fast and accurate method for diagnosis of MAP.

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