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Article
Detection of Porphyromonas gingivalis from Periodontal Pocket Infections by Microbial Cultivation and PCR Techniques
التحري عن جرثومة Porphyromonas gingivalis من إصابات جيوب ما حول الأسنان بطرق الزرع الجرثومي وبتقنية PCR

Author: Amera M. M. Al-Rawi أميرة محمود محمد الراوي
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2012 Volume: 23 Issue: 1A Pages: 39-55
Publisher: Mosul University جامعة الموصل

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Abstract

The study aimed to detect P. gingivalis from 49 patients with periodontitis at different ages and both sexes, after determination of pocket depth, types of infection whether chronic or progressive by dentists. Routine culture method was done using selective media and anaerobic condition and compared with species specific polymerase chain reaction (PCR) technique.DNA was extracted from samples and its concentration and purity were determined. The results showed domination of chronic infections and the pocket depths ranged between 3-9mm, as well as the results revealed that isolation percent of P.gingivalis by PCR was more higher than culture method, it was 65.3% and 28.5% respectively. The results also showed that phenol-chloroform was the efficient method for DNA extraction comparing with other methods. The study revealed that there are effects of age and sex on isolation rate and the results indicated that percentage of P.gingivalis was detected in 20-30 years old and males were more infected than females.

هدف البحث إلى عزل وتشخيص جرثومة P.gingivalis من 49 مريضاً مصاباً بالتهاب جيوب اللثة (ما حول الأسنان) و بأعمار مختلفة من كلا الجنسين بعد أن تم تحديد عمق الجيب Pocket depth ونوع الإصابة إما مزمنة Chronic أو متقدمة Progressive من قبل طبيب أسنان اختصاص بأمراض اللثة. تم استخدام الطرائق الروتينية بالزرع على الأوساط الانتخابية وتوفير ظروف لا هوائية وقورنت مع تقنية تفاعل البلمرة المتسلسل للنوع المتخصص (PCR)، إذ تم استخلاص الحامض النووي DNA وحدد تركيزه ونقاوته.بينت النتائج سيادة الاصابة المزمنة على المتقدمة وان عمق الجيب تراوح بين 3-9 ملم كما أظهرت النتائج أن نسبة الكشف عن الجرثومة بتقنية PCR اعلى بكثير مقارنة بنسبة عزلها والتحري عنها بالطرائق الزرعية إذ بلغت 65.3% و 28.5% على التوالي. كما ابدت النتائج ان طريقة الفينول-كلوروفورم الروتينية هي الاكفأ في استخلاص الـ DNA مقارنة بالطرق الأخرى.كما أظهرت الدراسة أن هناك تأثيراً لعاملي العمر والجنس على نسبة العزل فقد كانت اعلى نسبة عزل لجرثومة P.gingivalis عند الفئة العمرية 20-30 سنة وان الذكور اكثر اصابة من الإناث.


Article
Sensitivity of treponema denticola isolated from infected periodontal pockets to some mouth rinses and common antibiotic

Authors: Amera M. M. Al-Rawi --- Summaya A. S. Muhammad
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 3 Pages: 220-227
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: Treponema denticola is one of the most important periodontal pathogens because of its high lytic enzymatic activity leading to tooth lose and its ability to invade tissues and spread via blood stream causing systemic infections, this study coming to be the first local study concerning with this anaerobic fastidious bacterium and looking at the possibility of rely on the culturing methods in determining the most preferred chemotherapeutic drugs for limiting of bacterial activity and spread, as well as investigating the range of the antimicrobial activity of some mouth rinses and number of antibiotics. Materials and Methods: 139 samples were collected from periodontal pockets with a depth of 3 mm under supervision of specialist dentitist, then placed in a reduced transport medium. The activity and effective spectrum of different concentrations for three kinds of widely used mouth rinses in the treatment of periodontal infections including Biofresh K, Biofresh F and Zak towards this bacterium was studied using sensitivity test methods, furthermore, the minimum inhibitory cocentration of antibiotics amoxillin, ciprofloxacin, clindamycin, azithromycin and mitronidazole was determined using antibiotic discs diffusion method. Results: The results showed that Biofresh K is the best in the term of bacterial killing as its inhibition activity continued to 1:16 dilution and when the sensitivity of T.denticola isolates to some antibiotics was tested, it was appeared that ciprofloxacin is the best causing growth inhibition with the lowest minimum inhibitory cocentration (0.0001mg), and when the synergistic effect of the tested antibiotics was studied it is becoming clear that the lowest antibiotic concentrations can cause growth inhibition when the two antibiotics (mitronidazole + ciprofloxacin) or (mitronidazole + amoxillin) are used in combination. Conclusions: It is possible to depend on culturing methods for determining the sensitivity of the bacterium T.denticola to chemotherapeutic drugs. Biofresh K is the best among the rinses under study and the antibiotic CIP is the best one with the lowest MIC and the lowest concentration of the antibiotics resulting in growth inhibition can be achieved when they are used in combination


Article
Effect of Sex and Age of Human on Infection With Types of Stones and Its Concomitant Bacteria

Authors: Dr. Amera M. M. AL-Rawi --- Rash N.H.A. AL-Sa’doon
Journal: JOURNAL OF EDUCATION AND SCIENCE مجلة التربية والعلم ISSN: 1812125X Year: 2019 Volume: 28 Issue: 2 Pages: 122-132
Publisher: Mosul University جامعة الموصل

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Abstract

The current study involved isolation and identification of bacteria from (50) urine samples of patients suffering from renal stones and urinary tract infections (UTIs) Sixteen bacterial isolates were obtained from urine samples (32%), Proteus mirabilis and Escherichia coli being the most frequent among isolates, forming 37.5% (6 isolates) and 31.5% (5 isolates) respectively while Klebsiella pneumoniae isolates 12.5% (2 isolates) then Enterobacter aerogenes, Pseudomonas aeruginosa, Staphylococcus aureus were isolated at a rate of (6.25%).The study showed that 32 (64%) of urine samples were from patients having calcium stone, 10 (20%) with uric acid stone and 8 (16%) with struvite stone but cystine stones were not identified. Furthermore, this study showed that there is a relationship between type of stones and the type of bacterial isolate .The effects of some factors such as sex and age on the rate of infection with different stone types were investigated ,the results revealed that among (32) calcium stones, 71.9% were males and 28.1% were females. From (8) struvite stones, males represent 37.5% while females were 62.5%, for uric acid stone 60% were males and 40% were female.The incidence of renal stone seem to be the highest in the age group(30_53) years comprising 48% of the total patients followed by the age group (54_77) years 32% and the least incidence was in the age group (6_29) years 20% .


Article
Isolation and Morphological Identification of Oral Treponema from Infected Periodontal Pockets
عزل جراثيم Treponema الفم من جيوب ما حول الاسنان المصابة وتشخيصها مظهريا

Authors: Amera M. M. Al-Rawi أميرة محمود محمد الراوي --- Summaya A. S. Mohammed سمية عدنان صالح محمد
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2011 Volume: 22 Issue: 4A Pages: 58-70
Publisher: Mosul University جامعة الموصل

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Abstract

The study included the relying of some phenotypic tests to investigate the possibility of isolation and identification of oral Treponema from infected periodontal pocket samples. These phenotypic tests included the examination of fixed smears prepared from the bacterial isolates and staining them with gram and modified gram's stain and with Fontana stain under light microscope where the distinct spiral forms of germs were seen. The oral Treponemes also identified by the observation of their actively motile spiral cells in wet smears using phase contrast microscope. The isolation and identification of these bacteria also proved after their interaction with specific antibody and cell clarifying by greenish spiral form when illuminated with U.V. light using fluorescence microscope. Further, the isolation and identification of oral Treponema was continued by the removal of their outer membrane (O.M.) using Triton X-100 and releasing their flagella and staining with specific flagella stain.

تضمنت الدراسة اعتماد بعض الاختبارات التشخيصية المظهرية للتحري عن امكانية عزل جراثيم Treponema الفم من عينات جيوب ما حول الاسنان المصابة، شملت هذه الاختبارات فحص مسحات مثبتة محضرة من العزلات الجرثومية وصبغها بصبغة كرام وكرام المحورة وصبغة فونتانا تحت المجهر الضوئي حيث لوحظت الاشكال الحلزونية المميزة للجراثيم. كما شخصت Treponema الفم بملاحظة خلاياها الحلزونية المتحركة بنشاط في المسحات الرطبة باستخدام مجهر الطور المتباين. هذا وتم تأكيد عزل وتشخيص هذه الجراثيم بعد مفاعلتها مع الاجسام المضادة المتخصصة ووضوح خلاياها الحلزونية باللون الأخضر البراق عند تسليط ضوء UV عليها باستخدام المجهر المتألق، اضافة الى اننا تمكنا من ازالة الغلاف الخارجي (O.M.) باستخدام مادة Triton X-100 وتحرير اسواطها وصبغها بصبغة السوط الخاصة.


Article
Preparation of Laboratory Kit for Lipoteichoic acid as One of the Constituents of Gram-Positive Bacterial Cell Wall
تحضير عدة الفحص Kit مختبريا لحامض التيكويك الدهني Lipoteichoic acid، احد مكونات جدار الجراثيم الموجبة لصبغة كرام

Authors: Amera M. M. Al-Rawi أميرة محمود محمد الراوي --- Tuka M. Al-Sawaf تُقى مؤيد بكر الصواف
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2012 Volume: 23 Issue: 1A Pages: 1-10
Publisher: Mosul University جامعة الموصل

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Abstract

The study includes the production of antibody for LTA in the rabbit after isolation and purefication from S.aureus and St.viridans by injecting the animals regullary and consecutively with pure LTA. Kit was prepared to investigate the presence of antibodies in sera of injected animals by ELISA, the absorbance of the test samples for each of S.aureus - LTA and St.viridans - LTA were 1.0700 ± 0.0141 and 1.0100 ± 0.0141 respectively, The absorbance of control sample, which represent sera of lab animals injected with distilled water was 0.9300 ± 0.0141.

تضمنت الدراسة إنتاج الأجسام المضادة لـحامض التيكويك الدهني LTA في الأرانب بعد ان تم عزله وتنقيته من جرثومتي S.aureus و St.viridans ، وذلك بحقنها بجرعات منتظمة ومتتالية من معلق LTA النقي بكميات متزايدة تدريجيا من المعلق المحضر وحضرت عدة فحص Kit مختبريا للتحري عن وجود الأجسام المضادة في أمصال الأرانب المحقونة به بتقنية الاليزا ELISA إذ بلغت امتصاصية عينات الاختبار لكل من S.aureus - LTA وSt.viridans – LTA (0.0141 ± 1.0700 و 0.0141 ± 1.0100) على التوالي كما بلغت قيمة امتصاصية عينات السيطرة التي تمثل أمصال الحيوانات المختبرية المحقونة بالماء المقطر (0.0141 ± 0.9300).


Article
Effect of some chemicals and medicinal Plants on Swarming phenomenon of Proteus mirabilis
تأثير بعض المواد الكيميائية والنباتات الطبية العشبية على ظاهرة العج لجرثومة Proteus mirabilis

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Abstract

The study involved the effect of some chemicals and medicinal plants on swarming phenomenon of P. mirabilis , the study showed the efficiency of thymol extracted from thymus vulgaris as a bactericidal effect in different concentrations similar to the effect of Sodium Dodecyl Sulphate (SDS) and in the same concentration the study also pointed out the inhibitory effect of Urea on swarming which increased with increase the concentrations , while the herb Comiphora myrrha did not show any effect on swarming compared with the control sample.

شملت الدراسة تأثير بعض المواد الكيميائية والنباتات الطبية العشبية على ظاهرة العج Swarming لجرثومة P. mirabilis واظهرت الدراسة كفاءة مادة الثايمول Thymol المستخلصة من نبات الزعتر Thymus vulgaris في تأثيرها القاتل على الجرثومة وفي تراكيز مختلفة مشابهة لتأثير مادة (SDS) Sodium Dodecyl Suphate وبنفس التراكيز. كما أوضحت الدراسة إن تأثير مادة اليوريا Urea كعامل مثبط لظاهرة العج يزداد بزيادة التركيز المستخدم، أما مادة المرة مكي Comiphora myrrha العشبية فلم تظهر أي تأثير ايجابي على تثبيط العج بالمقارنة مع نموذج السيطرة.

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