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Article
Superficial Fungal infections

Authors: Auroba K. Abbas --- Zahraa A. Mohammed --- Imad S. Mahmoud
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2012 Volume: 11 Issue: 1 Pages: 75-77
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: This work aimed to isolate and identify fungi from different superficial fungal infection since these infections are widely distributed among low socioeconomic level people.Patients and Methods: All clinical specimens from skin, hair, scalp and feet were diagnosed as dermatophytes or other fungi according to well-known established mycological methods for isolation and identification of certain fungi. Results: This study was undertaken at AL-Yarmook Teaching Hospital during the period January 2008-December 2009 in which 55 clinically diagnosed as fungal infection were the source of clinical specimens. Out of 55 cases it has been found that 45 specimens (81.8%) were positive for fungi from which 21 (46.6%) were dermatophytes, 16 (35.5%) pityriases versicolor, 8(18.2%) candida albicans.Conclusion: It is concluded that dermatophytes are the major cause of mycotic superficial infections and that skin is the predominant area of the body which is invaded by fungi.


Article
Isolation and Identification of Fungi Associated with Otomycosis
عزل وتشخيص الفطريات الملازمة لالتهاب الاذن الخارجية

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Abstract

Objective and Background: Laboratory diagnosis of predisposing factors of otomycosis which represent a significant percentage of clinical external otitis and is usually caused by Candida, Aspergillus; penicillium, clinical symptoms such as otorrhea, erythematic and stenosis of the external auditory canal.Patients: Seventy-five patients were included in this study suffering from symptoms indicating otitis externa in Al-Yermouk teaching hospital, E.N.T. department during the period from November 2007 to April 2008.Methods: The specimens were taken by sterile cotton swabs from bony portion of the external ear. All specimens were cultured on sabouraud Dextrose Agar with chloramphenical (0.05mg/ml). Mycological Cultures were incubated at (25-27) ċ for 2-3 weeks and examined microscopically every 3 days.Results: In this study 75 patients (44 Female and 31 male) with symptomatic otomycosis were confirmed by direct microscopy and cultures. Results revealed that (45) 60% of patients (38 female and 7 male) showed association of fungi and Candida albicans. Female susceptibility to fungal infection was more than male 82.23%. The most common fungal pathogens isolated from ear were Aspergillus niger (44.44%) followed by Candida albicans (33.33%), and penicillium spp. (22.22%).Conclusion: In clinical finding of otitis externa, mycological examination could be very important in setting accurate diagnosis and appropriate therapy can be decided. In this study we found that otomycosis is common in female than male and A. niger is the major etiologic agent in ear infection.Keywords: Otomycosis, Aspergillus, Candida albicans, penicillium.

الهدف: هو التشخيص المختبري للفطريات المسببة لالتهاب الاذن والذي يشكل نسبة مهمة من التهاب الاذن الخارجية والمتسببة عن الفطريات وهي المبيضات المرضية Candida وAspergillus وPenicillium والاعراض السريرية بشكل عام وهي قيح جاري من الاذن واحمرار وانسداد الاذن الخارجية.طرق العمل : تضمنت الدراسة 75 مريض يعانون من اعراض تشير الى التهاب الاذن الخارجية في مستشفى اليرموك التعليمي وحدة الانف والاذن والحنجرة وفي الفترة من تشرين الثاني 2007 الى نيسان 2008. تم اخذ العينات بأستخدام مسحات قطنية معقمة من افرازات وقيح الاذن الخارجية وتم تنمية العينات على وسط Sabourad Dextrose Agar مع اضافة المضاد الحيوي البكتيري الكلورامفينكول وبتركيز (0.05 ملغم امل)و تم حضن المزارع الفطرية بدرجة (25-27)م ولفترة 2-3 اسابيع وتم فحصها مجهريا كل ثلاثة ايام.النتائج: في هذه الدراسة اثبت التشخيص المجهري والزرعي ول75 مريض اصابتهم بأعراض التهاب الاذن الفطري. لقد اظهرت النتائج ان 45( مريض بنسبة 60%) (38 اناث و7 ذكور) وملازمة واضحة للاصابة بالفطريات و(المبيضات المرضية Candida albicans ). كانت حساسية الاناث للاصابة بالفطريات اكثر من الذكور.Aspergillus niger كان اكثر الفطريا الممرضة شيوعا وعزل بنسبة 44,44% يليها (Candida albicans) بنسبة 33,33% ثم Penicillium spp (22,22%).الاستنتاج: ان التشخيص السريري للاتهاب الاذن الفطري يحتاج الى التأكيد بالتشخيص المختبري للفطريات مما يعطينا تشخيص دقيق ويساعد على تقرير العلاج المناسب. في هذه الدراسة وجد ان التهاب الاذن الفطري في النساء اكثر من الرجال وان A. niger هو من المسببات الرئيسة لالتهاب الاذن الفطري.

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