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Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique

Authors: Ban A. Abdul majeed** PhD --- Faiza A. Mukhlis* PhD --- Shatha F. Abdullah* FICM
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 2 Pages: 178-183
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can transform cells and dysregulate many cellular pathways involved in oncogenesis. This study was conducted to detect HCMV matrix-protein mRNA using In Situ Hybridization technique in glial brain tumor tissues compared to normal brain tissues and the presence of cytomegalic inclusion bodies in brain tumor tissues.Patients and Method: Thirty eight of glial tumor specimens were obtained in paraffin blocks compared to eight normal brain autopsy specimens which were age and sex matched with the study group as a control group. ISH was conducted tissue sections using a biotinylated Long DNA Probe for CMV Matrix Protein together with in-situ hybridization (ISH) detection kit.Results: The biotinylated probe specific for mRNA encoded HCMV – Matrix Protein showed hybridization with viral nucleic acids in 34 cases(out of 38)of malignant glial tumor specimens representing (73.9% ) of the total study groups . All cases with high grades astrocytoma revealed a positive hybridization in a percentage of 32.6% from 15 cases with grade III, and 10 ( 21.7% ) cases with grade IV astrocytoma. Nine out of 38 cases with grade III astrocytoma representing (23.7%), 7 (18.4 %) cases with glioblastoma multiforme and 2 oligodendroglioma cases(5.3 %), revealed inclusion bodies on histological examination. Conclusions: HCMV may play a role in the glioma pathogenesis. In Situ Hybridization test proved to be a very sensitive and specific technique for the detection of HCMV mRNA in tissues. Epidemiological, histopathological identification of cytomegalic inclusion bodies , and molecular studies are necessary to confirm the association of HCMV related human cancers in general Iraqi population.


Article
Detection of Transcription Factor C-MYC in Oral Dysplasia and Squamous Cell Carcinoma by in Situ Hybridization

Author: Ban A. Abdul-Majeed* PhD Molecular pathology. د. بان عباس عبد المجيد
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 4 Pages: 388-391
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: Oral squamous cell carcinoma continues to be a major health problem in Iraq as well as in other countries. Many attempts were made to study molecular markers in oral squamous cell carcinoma and to link them to tumor grade, stage and prognosis, besides studying their role in carcinogenesis. The present study has been designed to detect mRNA of c-myc in oral squamous cell carcinoma compared to oral dysplasia and to link the marker to grade and degree of the two pathologies.Materials and methods: Forty two cases, including 30 cases of oral squamous cell carcinoma and 12 cases of oral dysplasia were included in this study. Sections on positively charged slides were made from their paraffin blocks and were used for the detection of c-myc mRNA using in-situ hybridization technique.Results: C-myc mRNA was detected in 9(75%) cases of oral dysplasis and in 24 (80%) cases of oral squamous cell carcinoma. A significant correlation was found between c-myc mRNA score and intensity from one side and the tumor grade from the other side and degree of dysplasia. Conclusion: The results of this study confirm the role of c-myc in oral dysplasia and oral squamous cell carcinoma and the possible transfer from the former to the latter.Key Words: Oral squamous cell carcinoma, oral dysplasia and c-myc.

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