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Article
mRNA in situ hybridization analysis of VEGF in chronic lymphocytic leukemia

Authors: Haythem A. MJ Al-Rubaie --- Ban A. Abdul Majeed بان عباس عبد المجيد --- Abdulkareem M. Jaafar
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2011 Volume: 53 Issue: 2 Pages: 186-189
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Several factors render chronic lymphocytic leukemia (CLL) an interesting subject for study by researchers. These include marked progress in understanding the molecular biology of normal and neoplastic lymphocytes and recent advances in molecular genetics techniques. Among molecular markers, vascular endothelial growth factor (VEGF), have been widely studied.Objective: The aim of the study is to evaluate the role of VEGF in the pathogenesis of CLL and its role in disease progression.
Patients, materials and methods: A retrospective cross-sectional study was done on 60 patients with chronic lymphocytic leukemia (45 males & 15 females) compared with 20 controls (anemic patients), all recruited at the Medical City Hospital/ Teaching Laboratories/ Baghdad from January 2004 to December 2007. The bone marrow biopsy of each was re-examined histologically. In situ hybridization was performed utilizing biotin labeled VEGF cDNA probes.
Results: The frequency of VEGF positivity was 95% (57 of 60 cases). A statistically significant inverse correlation was found between increasing VEGF scores and lower packed cell volume level and lower platelet count. A statistically significant association was found between advanced modified Rai stage and patients with high score. In addition, there was a statistically significant positive correlation between increasing scores of VEGF and advancing clinical stage.
Conclusions: The score of VEGF is high among patients with advanced clinical stage of the disease.


Article
THE INFLUENCE OF N-RAS GENE MUTATIONS ON THE RESPONSE TO INDUCTION THERAPY IN AML IRAQI PATIENTS

Authors: Nahidh K. Alwan ناهض كامل علوان --- Raad J. Musa رعد جابر موسى --- Ban A. Abdul-Majeed بان عباس عبد المجيد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 3 Pages: 230-237
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:N-RAS mutations are the most commonly detected molecular abnormalities in hematologic malignancies, especially in those of myeloid origin.Objective:Current study aimed to determine the frequency of N-RAS mutation; and its influence on response to induction therapy in patients with acute myelogenous leukemia (AML) in Iraq.Methods:Peripheral blood and bone marrow samples were taken from 58 newly diagnosed AML patients and 30 individuals with reactive bone marrow were selected as a control group. Samples screened for N-RAS gene mutations using nested PCR were followed by mutation sensitive digestion analysis (MSDA).Results:N-RAS mutations at the time of diagnosis were found in 10/58 (17.24%) patients with AML and no mutation in control individuals. Patients with mutant N-RAS showed lower complete remission (CR) than wild type, the difference was not significant (60% vs. 72.92%, P = 0.414).Conclusion:The current results provide clues for activation of RAS-signaling cascade in AML patients, supporting their role in molecular pathogenesis of leukemia. N-RAS mutations show no influence on CR rate in AML patients. Further studies on larger scale to define the prognostic significance of N-RAS mutations are recommended.Keyword:AML, N-RAS mutation, MSDA, complete remission.

Keywords

AML --- N-RAS mutation --- MSDA --- complete remission


Article
ANALYSIS OF N-RAS GENE MUTATIONS AND P21N-RAS PROTEIN EXPRESSION IN IRAQI PATIENTS WITH IN AML

Authors: Nahidh K. Alwan ناهض كامل علوان --- Raad J. Musa رعد جابر موسى --- Ban A. Abdul-Majeed بان عباس عبد المجيد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 4 Pages: 329-339
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:N-RAS mutations are the most commonly detected molecular abnormalities in hematologic malignancies, especially in those of myeloid origin.Objective:Current study aimed to determine the frequency of N-RAS mutation and its correlation with P21N-RAS protein expression in patients with acute myelogenous leukemia (AML) in Iraq.Methods:Peripheral blood, bone marrow aspirate and biopsy samples were taken from 58 newly diagnosed AML patients (57 de novo and 1 therapy related AML) and 30 individuals with reactive bone marrow conditions were selected as a control group. Samples screened for N-RAS gene mutations using nested PCR followed by mutation sensitive digestion analysis (MSDA), and immunohistochemical analysis of P21N-RAS protein expression by using anti N-RAS monoclonal antibody.Results:N-RAS mutations at the time of diagnosis were found in 10/58 (17.24%) and P21N-RAS expression found in 5/58 (8.62%) patients with AML. There was a significant difference (P = 0.001) in P21N-RAS expression between mutant and wild type N-RAS patients with AML. No N-RAS mutations or P21N-RAS expression detected in the control group individuals.Conclusion:It can be suggested that there is activation of RAS-signaling cascade in AML patients, this is may support their role in molecular pathogenesis of acute leukemia. Also, there was a significant difference between N-RAS gene status and P21N-RAS protein expression in patients with AML.Keyword:AML, N-RAS Mutation, MSDA, P21N-RAS expression, Digital analysis.


Article
IN SITU HYBRIDIZATION APPROACH FOR DETECTION OF EPSTEIN –BARR VIRUS & HUMAN PAPILLOMAVIRUS TYPE 16 IN A GROUP OF IRAQI WOMEN WITH CERVICAL CARCINOMA
نهج التهجين الموضعي للكشف عن فيروس ابشتاين بار والفيروس الحليمي البشري نوع 16عند مجموعة من النساء العراقيات المصابات بسرطان عنق الرحم

Authors: Jasim Mohammed Muhsin جاسم محمد محسن --- Ban A. Abdul-Majeed بان عباس عبد المجيد --- Saja J. Al-Khalidi سجى جهاد الخالدي
Journal: AL-TAQANI مجلة التقني ISSN: 1818653X Year: 2014 Volume: 27 Issue: 2 Pages: E22-E31
Publisher: Foundation of technical education هيئة التعليم التقني

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Abstract

Aim: In order to investigate the involvement of (EBV & HPV 16) viruses in thepathogenesis of cervical carcinoma, the presence of the genome of these viruses wereinvestigated in 100 cervical biopsies, histologically classified as squamous cellcarcinomas & adenocarcinomas of uterine cervix. Methods: detection of DNA signals forboth EBV & HPV 16 by non-radioactive In Situ Hybridization technique (ISH). Results:A different percentage of positively signals for vital infection was found between the twogroups of examined DNA; EBV & HPV 16 infection in cervical carcinoma cases were52% and 70% respectively; on the contrary in normal cervix tissues a low percentage ofviral genomes presence were detected: 44% for EBV and 25% for HPV 16. Conclusions:The present study shows there is a compelling body of evidence that cervical carcinoma isrelated to human papilloma virus infection as a Co-factor and more recently Epstein-Barr virus have been proposed as candidates ( other Co-factore) for cervical neoplasia.Our data confirm the involvement of HPV16 & EBV in cervical neoplasia and suggest apossible role of these viruses in cervical cancerogenesis

الهدف من هذه الدراسة هو من اجل التحري عن مدى تداخل فيروس ابيشتاين بار والفيروس الحليمي البشري نوع 16 في التسبب بسرطان عنق الرحم , وجود الجينوم لكلا الفيروسين بحثت في 100 خزعة من عنق الرحم , والتي صنفت نسيجياً الى سرطان الخلايا الحرشفية والخلايا الغدية لعنق الرحم ,الطرق: استخدام تقنية التهجين الموضعي الغير مشعة للكشف عن اشارات الحمض النووي (الدنا) لكلا فيروس ابيشتاين بار وفيروس الحليمي البشري نوع 16.نتائج هذه الدراسة وجدت نسب اشارات موجبة مختلفة للأصابة بين المجموعتين الخاضعة لفحص الدنا , بحيث كانت النسب في حالات سرطان عنق الرحم هي 52 % و 70% لكلا فيروس ابشتاين بار و فيروس الحليمي البشري نوع 16على التوالي وعلى العكس اكتشفت نسب منخفضة للضهور الحيوي لجينوم الفيروسين في أنسجة عنق الرحم الطبيعية : 44٪ لل EBV و 25٪ لل HPV16. الدراسة الحالية وجدت مجموعة من الأدلة المقنعة على أن سرطان عنق الرحم يرتبط بأصابة فيروس الحليمي البشري كعامل مشترك وقد اقترحت مؤخرا فيروس ابشتاين بار كمرشح (عامل مشارك اخر) لنشوء اورام عنق الرحم. والبيانات المتوفرة لدينا تؤكد تورط HPV16 و EBV في اورام عنق الرحم و تشير إلى دور هذه الفيروسات في سرطنة عنق الرحم

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Article
Forkhead box P3 gene expression and chromosomal analysis in a sample of Iraqi patients with multiple sclerosis

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Abstract

Background: Multiple Sclerosis disease is a demyelination process which interferes with the neuronal signal transmission, thus leading to different cognitive and physical dysfunctions like optic neuritis, motor, sensory and coordination problems. Recently many researches have been directed toward studying the relation between some genes and multiple sclerosis. Among the important genes to be studied in multiple sclerosis is the forkhead box P3 gene expression.Objectives: The aims of the present work were to study the expression of forkhead box P3 gene by real time polymerase chain reaction, and to perform chromosomal analysis on the multiple sclerosis patients peripheral blood lymphocytes.Patients and methods: A case-control study was performed using real time polymerase chain reaction technique to measure the relative expression of forkhead box P3 gene in peripheral blood leukocytes of 25 newly diagnosed untreated multiple sclerosis patients and comparing them with that of 25 apparently healthy controls, in addition to performing chromosomal analysis on multiple sclerosis patients peripheral blood lymphocytes.Results: Significant down-regulation in forkhead box P3 gene was detected in multiple sclerosis patients in comparison to controls. Chromosomal analysis that was performed for multiple sclerosis patients revealed normal results.Conclusion: Multiple sclerosis is associated with significant change in forkhead box P3 gene expression that can be measured by real time polymerase chain reaction technique. Furthermore, multiple sclerosis is not associated with gross chromosomal abnormalities.Key words: Multiple sclerosis, Foxp3 gene, real time PCR, chromosomal abnormality.

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