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Seroprevalence of brucellosis and toxoplasmosis in camels of Wasit Province, Iraq

Author: Basim Mohammed Hanon
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 29-37
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Background: toxoplasmosis and brucellosis are zoonotic diseases, more added a major public health is worldwide because have high distribution in livestock. Which affects social and economic development in developing countries. Objectives: The aim of this study research was to determine the occurrence of the seroprevalence toxoplasmosis and brucellosis in camels in Waist provinces of Iraq from November 2016 to April 2017. Materials and Methods: Overall (237) blood samples collected of animals randomly were from both sex in different herds of animals and diagnosis by A Latex agglutination test (LAT), Rose Bengal Plate Test (RBPT) and indirect (ELISA). Results: An overall prevalence of T. gondii infestation were recorded, the positive sample with LAT test was 76 (32.06%) from all sample, the results of ELISA was shown in different groups 24.14% (7), 30.55% (11), 26.67% (8), 20% (8), 20% (15), 25.9% (7) from group1 to 6 respectively, while in age groups ELISA results appeared 10.71% (6) , 64.29% (36), 25% (14) respectively, the seroprevalence in females 50 (89.18 %) and positive in males 6 (10.72%). While brucellosis RBPT 51 samples positive and 186 sample negative, among 51 positive by RBPT confirmed by ELISA 39 sample positive and 198 negative, This positive sample divided in to 6 livestock groups, from 1 to 6 groups (5) 17.25%, (4) 11.10%, (8) 20%, (7) 17.5%, (10) 13.4%, (5) 18.5%, respectively with final percentage 16.29% while the negative result percentage 83.71%, high seroprevalence was recorded in moderate age (24) 10.12 % and the older than 10 years age (9) 3.79% while the less percentage in group 1under 5 years of age (9) 2.53 %, while the seroprevalence recorded a higher percentage in females (1) 97.43 % and less recorded in male (1) 2.57%.The two tests were used ELISA 56 positive samples (23.62%) and 181(76.28%) negative samples. However, 76 (32.06%) positive by LAT test and 161(67.94%) negative. Statically in (P>0.05), non-significant was obtained in infection between groups of camels in this study and between the sex in all camels groups. Conclusions: high seroprevalence in studied camels indicated the importance of these animals as the main source of human infection. The widespread infection of other livestock. Clinical signs alone are not sufficient for diagnosis. Difficulties can arise in chronic camel infection.

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Article
Investigation of Microsatellite Instability BAT 25 and BAT 26 in Breast Cancer Patients by Conventional PCR

Authors: Jabbar Salman Hasan --- Basim Mohammed Hanon --- Ahmed Hasan Mohammed
Journal: Journal of Education for Pure Science مجلة التربية للعلوم الصرفة ISSN: 20736592 Year: 2017 Volume: 7 Issue: 3 Pages: 161-170
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Abstract:Background: Breast cancer is the most common cancer among women worldwide, comprising 23%of the 1.1 million female cancers that newly diagnosed each year.Aims: Investigate the existence of microsatellite instability (MSI) in breast cancer of patients.Settings and Design: Fifty female patients with invasive ductal breast carcinoma (IDC) collected.Inclusion criteria of subjects include female patients with diagnostic feature of breast cancer and agerange 26-42 years old untreated with chemotherapy or hormonal therapy.Methods and Material: DNA had be extracted from frozen tissue samples of breast cancer. Thisprotocol done according to the kit manufacture’s manual of QIAamp DNA Mini kit from Qiagen –USA. All samples tested for MSI bysingleplex PCR reactions using two microsatellite markers BAT25 and BAT 26.PCR achieved in a final volume of 50 μl and after thermal cycles, gel visualizationperformed.Statistical analysis used: The significance of differences in proportions was analysed by the Fisher’sexact testwith SPSS version 20 and P values equal or less than 0.001 considered statisticallysignificant.Results: PCR demonstrating microsatellite instability in 13 (26%) of the 50 breast cancer sample.Eight (16%) of 50 breast cancer sample were BAT 25 positive with a PCR product size of 124 bp,while 5 (10%) of 50 breast cancer sample were BAT 26 positive with a PCR product size 121bp.Conclusions: The result suggest strong evidence that microsatellite instability (MSI) at the BAT 25and BAT 26 and have involved in the pathogenesis of the great majority of breast cancers.

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