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Abstract: The study was conducted in AL-Kadhumia hospital for children, AL-Kadhumia city / Baghdad, through period April-July 2014. Mid –stream urine of 75 patients were cultured. Out of these 67(89.33%) patients` urine were positive for bacteriological culture. The distribution of infection was 50.7% infants aged 2-9 months and 46.3% children aged 1-4 years. Total of six bacterial species isolated were E.coli 35(52.2%), Enterobacter spp. 16(23.9%), Proteus spp.11 (14.9%), Klebsialle spp. 3(4.7%), Pseudomonas spp. 1 (1.5 %) and Staphylococcus spp. 1(1.5 %). The isolated organisms were identified based on conventional bacteriological and biochemical analyses and were characterized. PCR technique was used to detect Proteus vulgaris by using species – specific primers Urease C, the results of primer urease C observe 3 isolate were positive. Rapid diagnosis of the pathogen in a clinical sample is always very important.

uropathogens --- pediatric --- UTI --- proteus vulgaris --- urease C