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Article
Genotyping of HLA-class-I and II by PCR-SSP of Patients with Recurrent Aphthous Stomatitis
التنميط الجيني لمستضدات الخلايا البيضاء البشرية الصنف الاول و الثاني بواسطة سلسلة تفاعل أنزيم البلمرة و بتقنية البادئ المعين لمرضى ألتهاب الفم القلاعي المتكرر

Author: Batool H. Al-Ghurabei بتول حسن الغرابي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2012 Volume: 54 Issue: 1 Pages: 73-78
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Recurrent aphthous stomatitis is a common condition characterised by recurrent episodes of oral ulceration. Genetic factors have been implicated by numerous studies on the association of recurrent aphthous stomatitis and the genetically determined HLA subtypes. Objectives: Current study was established to shed light on the possible association of HLA class I and II alleles with recurrent aphthous stomatitis in Iraqi patients.Subjects and Methods: The study included 55 subjects: 30 recurrent aphthous stomatitis patients and 25 apparently healthy subjects as control. Polymerase chain reaction-specific sequence primers (PCR- SSP) assay was conducted to assess HLA- typing.Results: The present study showed a significant association of HLA-Cw*12:02:01-and HLA-DQB1*02:01:01- alleles with recurrent aphthous stomatitis as compared with healthy control (P= 0.041and P=0.028 respectively). Another interesting finding in this study was the significant low frequency of HLA-DQB1*05:01:01- allele in patients when compared with healthy control (P=0.036).Conclusion: These findings demonstrated that HLA-Cw*12:02:01- and HLA-DQB1*02:01:01-alleles may play a role in the etiology of the disease. Whereas HLA-DQB1*05:01:01-05 may confer protective effects against recurrent aphthous stomatitis.

الخلفية العلمية : التهاب الفم القلاعي المتكرر هو حالة شائعة تتميز بنوبات متكررة من تقرحات الفم. العوامل الوراثية قد تسبب هذا المرض وذلك من خلال دراسات عديدة بشأن ألأرتباط بين التهاب الفم القلاعي المتكرر وانواع من مستضدات الخلايا البيضاء البشرية المحددة وراثيا.أهداف الدراسة: صممت هذه الدراسة لإلقاء الضوء على وجود علاقة محتملة لبعض اليلات مستضدات الخلايا البيضاء البشرية الصنف الأول و الثاني مع التهاب الفم القلاعي المتكررفي المرضى العراقيين. الاشخاص وطرائق العمل : شملت الدراسة 55شخص: 30 مريض مصاب بالتهاب الفم القلاعي المتكرر و25من الاصحاء كمجموعة سيطرة. التنميط الجيني لاليلات مستضدات الخلايا البيضاء البشرية بطريقة سلسلة تفاعل أنزيم البلمرة و بتقنية البادئ المعين لسلسلة جينية PCR-SSP النتائج: أظهرت الدراسة الحالية وجود ارتباط معنوي بين الاليليين: HLA-DQB1*02:01:01و HLA-CW*12:02:01 مع المرضى وبقيمة احتمالية: (P= 0.041and P=0.028) على التوالي مقارنة بمجموعة السيطرة. نتيجة أخرى مهمة في هذه الدراسة هو انخفاض تكرار الاليل: في المرضى بالمقارنة مع مجموعة السيطرة و بقيمة أحتمالية: HLA-DQB1*05:01:01(P=0.036).: الأستنتاجات: هذه النتائج أظهرت أن الأليلينHLA-DQB1*02:01:01و HLA-CW*12:02:01قد يلعبا دورا في التسبب بهذا المرض. بينما الاليل:قد يضفي آثار وقائية ضد التهاب الفم القلاعي المتكرر. HLA-DQB1*05:01:01


Article
Evaluation of serum anti-Cardiolipin antibody, hs-CRP and IL-6 levels in chronic periodontitis as possible risk factors for cardiovascular diseases

Author: Batool H. Al-Ghurabei بتول الغرابي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 2 Pages: 161-165
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: It has long been established that there is an association between cardiovascular disease andperiodontitis. Evidence shows periodontally infected patients may be at a higher risk of thrombotic accidents viainduced systemic inflammatory mediators’ production and increase in serum levels of autoantibodies such as anticardiolipinantibody. The aim of the present study was to determine the presence of anti-cardiolipin antibody(ACLA)-IgG and -IgM, and to investigate the systemic levels of inflammatory markers of cardiovascular diseases likehigh sensitivity C-reactive protein (hs-CRP) and interleukin-6 (IL-6) in patients with chronic periodontitisin (CP) as wellas to examine the relationships between these mediators and clinical periodontal parameters.Subjects and Methods: Blood samples were collected from 45 patients with CP (20 with sever periodontitis and 25with moderate periodontitis) and from 30 healthy age matched individuals served as controls. Clinical periodontalparameters used in this study were plaque index, gingival index, probing pocket depth, clinical attachment leveland bleeding on probing. The levels of serum ACLA-IgG, ACLA-IgM, hs-CRP and IL-6 were determined using enzymelinkedimmunosorbent assays.Results: The current results revealed that serum levels of ACLA-IgG, hs-CRP and IL-6 were significantly higher inpatients group as compared to healthy control group (p<0.05, p<0.001), whereas the serum level ACLA-IgM was notobserved any significant differences between two groups (p>0.05). Concerning the comparison between twopatient groups, sever CP group showed significant elevation in serum levels of ACLA-IgG, hs-CRP and IL-6 (p<0.05,p<0.001), while there is no differences in serum level of ACLA-IgM when compared to moderate CP patients group(p>0.05). Furthermore, in regards to the correlation between serum ACLA-IgG, ACLA-IgM, hs-CRP and IL-6, andclinical periodontal parameters, IL-6 level was showed significant positive correlation with clinical attachment level,whereas hs-CRP was showed significant positive correlation with each of probing pocket depth, clinical attachmentlevel and bleeding on probing. Moreover; linear positive correlation was noticed between ACLA-IgG and clinicalattachment level. Conversely, ACLA-IgM level did not show any correlation with clinical parameters of periodontitis(p>0.05).Conclusion: Elevation in prothrombotic autoantibodies, ACLA-IgG and inflammatory mediators (hs-CRP and IL-6)factors may increase inflammatory activity in atherosclerotic lesions and potentially increasing the risk forcardiovascular events


Article
Role of SalivaryTumor Necrosis Factor-alpha and Immunoglobulin-Ain Recurrent Aphthous Stomatitis

Authors: Mustafa M. Saliah مصطفى محمد صالح --- Batool H Al-Ghurabei بتول الغرابي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2011 Volume: 53 Issue: 2 Pages: 207-210
Publisher: Baghdad University جامعة بغداد

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Abstract

Background:Until today, the etiology of recurrent aphthous stomatitis (RAS) remains unknown, although hints of its etiologic basis lay on genetic susceptibility, infectious agents and alterations in immune mechanics.Objectives:The aim of this study was to investigate the possible alterations in salivarytumor necrosis factor-alpha (TNF-α) and Immunoglobulin-A (IgA) level in patients with RAS and its relation with clinical types of disease.
Subjects and Methods:Salivary TNF-αlevels were investigated in50 RAS patients and 25 healthy controls by enzyme-linked immunosorbent assay (ELISA). Single radial immune diffusion method was used for estimation ofIgAlevel in two studied groups.
Results:Salivary level of TNF-α was significantly higher in RAS patients than in healthy controls (p<0.001), Moreover, the level of TNF-α was significantly increased in minor type of disease than in major and herptiform types (p<0.05).On the other hand, the levelof IgA showed no significant differences between patients and healthy controls and neither among clinical types of disease(p>0.05).
Conclusion: These findings suggestthat saliva provides an ideal medium for the detection of pro-inflammatory markers of the oral cavity, moreover; salivary TNF-α may play an important role in pathogenesis of this disease and it may also have an important role in the search of new treatments for this disease. As well as these results indicated to a possible role of mucosal immune system in pathogenesis of RAS.


Article
Study of HLA Class I and Class II by (PCR-SSP) and the role of salivary TNF-α in Iraqi patients with Recurrent Aphthous Stomatitis

Authors: Mustafa M. Salah مصطفى صلاح --- Batool H. Al-Ghurabei بتول الغرابي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 1 Pages: 151-157
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Until today, the etiology of recurrent aphthous stomatitis remains unknown, although hints of its etiologicbasis lay on genetic susceptibility, infectious agents and alterations in immune mechanics. Current study wasestablished to shed light on the possible association of human leukocyte antigen class I and II alleles with recurrentaphthous stomatitis, and to investigate the possible alterations in salivary tumor necrosis factor-alpha level in patientsand its relation with clinical types of disease.Subjects and Methods: The study included 55 subjects: 30 recurrent aphthous stomatitis patients and 25 apparentlyhealthy subjects as control. Polymerase chain reaction-specific sequence primers assay was conducted to assesshuman leukocyte antigen-typing whereas salivary tumor necrosis factor-alpha level was estimated by enzyme-linkedimmunosorbent assay.Results: The present study showed a significant association of HLA-Cw*12:02:01-and DQB1*02:01:01- alleles withrecurrent aphthous stomatitis as compared with healthy control, and there was significant low frequency ofDQB1*05:01:01- allele in patients when compared with healthy control. Furthermore, high frequency ofDQB1*02:01:01- alleles was observed among patients with minor type of recurrent aphthous stomatitis whencompared with healthy control. Another interesting finding in this study was the significant elevation of salivary tumornecrosis factor-alpha level in patients than in healthy controls, as well as strong association of high salivary tumornecrosis factor-alpha level among patients who expressed DQB1*02:01:0-allell was observed.Conclusion: Cw*12:02:01- and DQB1*02:01:01-alleles may played a role in the etiology of the disease, whereasDQB1*05:01:01-05 may confer protective effects against recurrent aphthous stomatitis. Moreover; salivary tumornecrosis factor-alpha may play an important role in pathogenesis of disease, and may also have an important role inthe search of new treatments for disease

Keywords

RAS --- HLA allele --- PCR --- Salivary TNF-á


Article
Effect of Methotrexate on Serum Levels of IL-1α and IL-8 in Rheumatoid Arthritis

Authors: Ahmed A. Al-Hassan --- Mohammed O. Hamzah --- Batool H. Al-Ghurabei
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 3 Pages: 404-408
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

BACKGROUND: Pathogenetic therapy of RA is a complex task,according to modern views on the development of* Dept. of Microbiology, Medical College, Al-Nahrain Univ. ** Dept. of Clinical Biochemistry, Medical College, Al-Nahrain Univ. *** Dept of Basic Science, College of Dentistry, Univ. of Baghdad. (1). autoimmune inflammation in RA, cytokineimbalance plays a great part in RA pathogenesis Rheumatoid arthritis is a chronic systemic inflammatory disease affects many tissues and organs, butprincipally attacks flexible (synovial) joints. Methotrexate is the most commonly used disease-modifyingantirheumatic drug for the treatment of rheumatoid arthritis. OBJECTIVE:The aim of this study was to evaluate the effect of methotrexate on serum levels of IL-1α and IL-8 inrheumatoid arthritis. SUBJECTS AND METHODS: Blood samples were collected from 50 patients with rheumatoid arthritis (25 patients without treatment and 25 patients are received methotraxate) and from 30 healthy age and sex matched individuals served ascontrols. Serum IL-1α and IL-8 were measured by means of enzyme-linked immune-sorbent assay.RESULTS: The present results showed that serum levels of IL-1α and IL-8 were significantly higher in RA patientsthan in healthy controls (P<0.01), furthermore, level of IL-1α was significantly decrease in patientstreated with methotraxate as compared to those patients who have received no treatment (P<0. 01). On theother hand serum level of IL-8 did not showed any significant differences between patients treated withmethotraxate and those patients without treatment (P>0. 05).CONCLUSION: These finding demonstrate that methotrexate turns out to be a good inhibitor for IL-α production. Inaddition, IL-1α and IL-8 may have a significant role in the pathogenesis of rheumatoid arthritis, and couldbe use a


Article
Quantitative analysis of IgG antinuclear antibody in chronic periodontitis patients

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Abstract

Background: Periodontitis is a bacterial infection of tooth-supporting tissues; the immunopathologic mechanismsinclude inflammatory cells and chemical mediators, which persist inflammation and develop a local autoimmune.The presence of autoantibodies against extracellular matrix components, anti-neutrophil cytoplasmic antibodies(ANCA) and anti-DNA was detected. This study aimed to provide evidence of altered humoral immune response inchronic periodontitis, as well as to determine the presence of auto-antibodies in this disease.Subjects and Methods: Blood samples were collected from 35 patients with chronic periodontitis (20 with severperiodontitis and 15 with moderate periodontitis) and from 30 healthy age and sex matched individuals served ascontrols. Clinical periodontal parameters used in this study were plaque index, gingival index, probing pocket depth,clinical attachment level and bleeding on probing. The levels of serum IgG-antinuclear antibody were determinedusing enzyme-linked immunosorbent assays, whereas serum immunoglobulin (IgG, IgM and IgA) were estimated bysingle radial immune diffusion method.Results: Serum levels of IgG-antinuclear antibody and IgG were significantly higher in sever chronic periodontitis thanin moderate chronic periodontitis and healthy controls (p<0.05). On the other hand, the serum levels of IgM and IgAshowed no significant differences among three studied groups (p>0.05). Concerning the correlation between serumIgG-antinuclear antibody and clinical periodontal parameters, the level of this autoantibody did not show anycorrelation with clinical parameters of periodontitis (p>0.05).Conclusion: The production of antibodies against self structures could be involved in the pathogenic mechanism ofchronic periodontitis

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