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Article
Antimicrobial effect of propolis on Streptococcus mutans

Author: Ghada Y ABDUL-RAHMAN
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2002 Volume: 2 Issue: 3 Pages: 299-303
Publisher: Mosul University جامعة الموصل

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Abstract

We choose two types of propolis extracts; watery and ethanolic; to estimate their activity against the most cariogenic microorganism; Streptococcus mutans, and evaluate this activity in vitro compared with chlorohexidine and tetracycline as negative controls, using the turbidity method, Both watery extract of propolis - WEP and ethanolic extract of propolis - EEP - show good inhibitory effect with significant variance compared with chlorohexidine, which also had better effect than tetracycline against Streptococcus mutans. The WEP at (1%) was the best antimicrobial solution in this study.


Article
Neomycin sulfate: A new antifungal and antiadherent agent against Candida albicans cells on heat–cured acrylic resin denture base material. An in vitro study

Authors: Ghada Y Abdul–Rahman --- Nagham H Kassab
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2003 Volume: 3 Issue: 2 Pages: 139-142
Publisher: Mosul University جامعة الموصل

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Abstract

Neomycin sulfate is an antibacterial agent. In this research, the antifungal and antiadherent effect of neomycin sulfate on Candida albicans that colonize the heatcured acrylic resin denture base material was evaluated. The results showed a significant effect compared with the control group. This effect is similar to the effect of the known antifungal agents (fluconazol and chlorhexidine) and lesser than (nystatin).


Article
Actinobacillus actinomycetemcomitans in periodontal infections

Authors: Ghada Y Abdul–Rahman --- Blend S Abdullah
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2006 Volume: 6 Issue: 3 Pages: S79-S83
Publisher: Mosul University جامعة الموصل

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Abstract

To isolate and identify Actino–bacillus actinomycetemcomitans because of its importance in periodontal diseases, to show their incidence in periodontal infections, and to study their important virulence factors. Materials and Methods: The isolating procedure had been done through using the selective Dentaid–1 medium, with anaerobic cultivation at 37 °C for 48 hours, and different microscopic features and biochemical activities were carried out. Also the study included the detection of virulence factors that owned by this type of bacteria through various tests. Results: The rate of infection was 45% in patients suffering from periodontal disease and the isolated bacteria had the ability to adhere to human epithelial cells of the mouth, also had the ability to produce bacteriocins as the substantive factors for the bacterial growth among the closer species of bacteria, and the presence of capsule, which was the most important virulence factor. Susceptibility of the isolated bacteria to selected types of antibiotic showed that 100% of the isolates were sensitive to tetracycline and ciprofloxacin, 100% were resistant to erythromycin and vancomycin. Conclusion: Actinohatillus actinomycetemcomitans was very important periodontal pathogen; having a large number of pathogenic and virulence factors; ciprofloxacin is the drug of choice.


Article
An Evaluation of the Antibacterial Effects of Rue and Sage Extracts

Authors: Siba M Al–Haliem --- Maha M Al–Bazzaz --- Ghada Y Abdul– Rahman
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2010 Volume: 10 Issue: 1 Pages: 55-61
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To investigate the antimicrobial effect of different concentrations of ethanolic and aqueous extracts of Sage and Rue (0.5%, 0.05%, 0.005%) on bacterial samples. They were iso-lated from root canal system in vitro, and the results were compared with that of chlorhexidine glu-conate (0.2%) as a positive control and normal saline as a negative control. Materials and Methods: Eighteen hours cultured bacterial samples of the root canal were used. To each 4ml of Brain Heart Infusion Broth (BHI), 0.1 ml of both bacterial sample and the examined solution Sage, Rue and chlorhexidine were added. Cultures were incubated at 37°C for 48 hours. The absorbance val-ues of the cultures were determined by the turbidity method using spectrophotometer at (550 nm). Re-sults: The results showed that both ethanolic and aqueous extracts of Sage and Rue have anti-bacterial effects in all concentrations with a significant variance compared with chlorhexidine against root canal microorganisms, while normal saline failed to show any antimicrobial activity. The results also showed that the ethanolic extracts of both plants have a higher antibacterial activity than the aqueous extracts. Conclusions: Rue and Sage demonstrated antibacterial effects on the root canal microorganisms (both aerobic and anaerobic) when compared with chlorhexidine.

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Article
Disinfection of Extracted Teeth for Den-tal Researches

Authors: Arjwan M Shukur --- Wiaam MO Al-Ashou --- Ghada Y Abdul-Rahman
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2010 Volume: 10 Issue: 1 Pages: 158-161
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To determine the effectiveness of different disinfection methods on extracted human teeth using five types of bacteria Proteus species, Escherichia coli, Kelebsiella species, Staphylococcus aureus, Streptococcus mutans. Materials and Methods: In this study extracted non-carious teeth were divided into five groups according to the type of bacteria that were inoculated inside the pulp chambers. Each group of the teeth were subdivided into six groups; group A: teeth were immersed in 2.5% NaOCl for 1week, group B: teeth were immersed in 1% NaOCl for 1week. Group C: teeth were autoclaved at 121C for at 15 Ibs psi for 15 minutes, group D: teeth disinfected using microwave for 3 minutes, group E: teeth disinfected using microwave for 6 minutes, group F: control group in which the teeth im-mersed in normal saline for seven days at room temperature. Each tooth aseptically placed in individual test tube with growth media. Simples were examined after 24h. Results: showed that autoclave, mi-crowave (when used at sex and three minutes), sodium hypochlorite at 2.5% prevented the growth completely in all types of the bacteria that were used to infect the teeth involved in this study.

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Article
The Antimicrobial Effect of Honey as Intracanal Medicament (A Comparative Study)

Authors: Rajaa T Sulieman --- Ghada Y. Abdul- Rahman --- Abdul-Khalik K AL-Sheik Abdal
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2009 Volume: 9 Issue: 1 Pages: 105-109
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: The current study aims to evaluate the antimicrobial effect of honey as a root canal medicament,
and to make a sort of comparison with the currently used root canal medicament (formocresol).
Materials and Methods: Fifty two uniradicular teeth were chosen. Microbiological sample was
obtained from the root canal at the beginning of the first appointment, instrumentation and irrigation of
the root canal followed by good dryness for the root canal, application of intra canal medicament depending
on the patient group, temporary dressing for the tooth, the samples were then transferred for
microbiological study.At the beginning of the second appointment (2-3 days later), the tooth was isolated,
the temporary dressing was removed and the microbiological samples of the root canal contents
were taken and complete the same sequence in the same manner as previously mentioned with the first
appointment. The sample transferred for microbiological study .The same procedure was done at the
beginning of the third appointment (2-3 days later) from the second appointment, the microbiological
study was done in the Microbiology Laboratory, Department of Dental Basic Sciences, College of Dentistry,
University of Mousl. Results: Comparing the antimicrobial effect between solutions of honey,
formocresol, there is no significant differences between these materials when used as root canal medicament.
Conclusion: This current study revealed that (H4 20% V/V) honey solution has antimicrobial
effect when used as intracanal medicament.

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Article
Preparation of a New Medium for the Isolation of Viridans Streptococci
تحضير وسط زرعي جديد لعزل المكورات السبحية الخضراء

Authors: Soubhi H. Khalaf صبحي حسين خلف --- Ghada Y. Abdul-Rahman غادة يونس عبد الرحمن
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2005 Volume: 16 Issue: 8A عدد خاص بعلوم الحياة Pages: 230-236
Publisher: Mosul University جامعة الموصل

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Abstract

Three types of media were prepared from the crude extract of pumpkin fruit, the first medium was prepared from the crude extract alone (Medium A1), the second was made by the addition of peptone to the crude (Medium A2) and the third was made by the addition of trypan blue, crystal violet and potassium tellurite (Medium A3) as selective agents for the isolation of S. mutans, these three types of media was examined for the growth and isolation of the Viridans Streptococci and S. mutans, all of them were good and there was no need to the addition of peptone (M. A1), the growth of S. Salivarius and S. mutans was differentiated from other Viridans Streptococci from saliva sample, by their large colonies, the A3 was selective for S. mutans by gummy, large and smooth colonies.

تم تحضير ثلاثة اوساط زرعية من مستخلص ثمار نبات القرع العسلي الخام، الاول من المستخلص الخام وحده (وسط A1) والثاني باضافة مادة الببتون كمصدر بروتيني (وسط A2) والثالث باضافة صبغات التريبان الازرق والبنفسجي البلوري ومادة تيلورات البوتاسيوم (A3) كمواد انتخابية لعزل المكورات السبحية الطافرة Streptococcus mutans، وعند اختبار قدرة الاوساط الثلاثة لانماء وعزل المكورات السبحية الخضراء Viridans Streptococci والمكورات السبحية الطافرة S. mutans كانت الاوساط الثلاثة جيدة حيث لم يكن هناك أي حاجة لاضافة الببتون (وسط A1) حيث كان نمو الجراثيم في عينة اللعاب واضحاً على هذا الوسط وبشكل تفريقي اذ ظهرت عزلات S. mutan و S. Salivarius كبيرة وبقية العزلات صغيرة وعند استخدام الصبغات الانتخابية في وسط A3 اصبح انتخابيا لجراثيم S. mutans متميزة بانتاج متعدد السكريات خارج خلوي بشكل مستعمرات علكية كبيرة ولماعة.

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Article
Effect of Microwave Disinfection on Transverse Strength and Hardness of Acrylic Resin Denture Base Materials

Authors: Ghada Y Abdul-Rahman --- Munther N Kazanji --- Alaa' N Al-Saraj
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 2 Pages: 284-291
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To evaluate the effect of microwave disinfection on transverse strength and hardness of acrylic resin denture base materials. Materials and methods: The samples were 112 samples (56 heat cured acrylic resin and 56 microwave cured acrylic resin) they were prepared in dimensions of 65± 0.3x10±0.03x2.5 ±0.03 mm (length, width and thickness respectively) for transverse strength test and 30x15x3±0.03 mm (length, width and thickness respectively) for indentation hardness test. The sam-ples were divided into seven disinfection groups; each one contain five samples which were (control, 900 W or 540W for 3min either inside glass flask containing 150 ml of distilled water)or along with glass flask containing 150 ml of distilled water or alone. The collected data were analyzed using analy-sis of variance (one way ANOVA) at P ≤ 0.05 and Duncan's multiple range test. Results: the transverse strength of microwave acrylic resin samples were significantly decreased except samples that disinfect-ed by 540 watt microwave energy for 3 minute with water inside microwave oven. The hardness num-ber for heat cured acrylic denture base showed no significant differences compared to control group while hardness number of microwave acrylic resin were significantly decreased in relation to control group. Conclusions: Transverse strength of microwave acrylic resin that disinfected with microwave was decreased except at 540 watt for 3 minutes with water a side in microwave oven. While heat cured acrylic resin after microwave disinfection was significantly increased compared with the control sam-ple. Rockwell hardness number for microwave acrylic resin significantly decrease in relation to control group; while for heat cured acrylic denture base showed no significancy comparing with the control group.


Article
Prevalence of Candida albicans in patients using fixed orthodontic appliances after the use of peppermint

Authors: Ghada Y Abdul–Rahman --- Zaid B Al–Dewachi --- Alaa D Al–Dawoody
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2005 Volume: 5 Issue: 1 Pages: 91-96
Publisher: Mosul University جامعة الموصل

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Abstract

This study was conducted to evaluate the antifungal effectof peppermint extract as mouth rinse during orthodontic treatment.Thirty patients (11 males and 19 females) who wear orthodonticappliances and instructed to use chlorhexidine asmouth rinse (8 patients); Mentha piperita extract (10 patients)or left to care of their teeth without any mouth rinse (12 patients);mean number of Candida albicans colonies recoveredfrom anterior surface at three intervals (Time 0: Before wearingthe appliance; time 1: After 7 days and time 2: After 1month) was 48.8, 144.3, 158.9 for the control group; 132.9,125.4, 147.9 for the peppermint group and 72, 105.8 and130.8 for the chlorhexidine group.The mean number of Candida albicans colonies fromthe posterior surface at the different intervals was 87.4, 136.8,159.5; 121.3, 84, 34.6 and 78.5, 91.25, 91 for control, peppermintand chlorhexidine groups respectively. The use of thepeppermint extract decreases the number of Candida albicanssignificantly at the posterior surfaces


Article
Isolation and Identification of Microorganisms from Patients with Halitosis
عزل وتشخيص الجراثيم من المرضى المصابين ببخر الفم

Authors: Ghada Y. Abdul-Rahman غادة يونس عبد الرحمن --- Rafit J. Al-Adole رافد الدولة
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2018 Volume: 27 Issue: 5E-Microbiology Pages: 1-7
Publisher: Mosul University جامعة الموصل

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Abstract

Twenty-eight patients suffering from oral halitosis were subjected to this study (14 males and 14 females), their ages range between 18 to 65 years were diagnosed clinically. Ninety-five samples were collected by sterile paper points (size 50) or sterile cotton swab and transported in thioglycolate broth and cultured on blood agar in aerobic or anaerobic conditions for (48-72 hours), the identification of the bacteria was carried out using morphological and cultural characteristics, biochemical tests and antibiotics susceptibility tests. The results showed that 13 different bacterial species isolated in this study ((Bacteriodes spp. (14 isolate), Viridans Streptococci (10 isolates), Peptostreptococci spp. (9 isolates), Actinomyces spp. (6 isolates), Porphyromonas spp. (4 isolates), Fusobacterium spp. (4 isolates), Veillonella spp. (4 isolates), Non.coagulase Staphylococcus (3 isolates) Prevotella spp (one isolate), Propinobacterium spp. (one isolate) Tetragenococci spp. (one isolate), Eubacterium spp. (one isolate), and Staphylococcus aureus (one isolate). The conclusion is the dominance of different genera and species of anaerobic bactria in cases of halitosis.

تم تشخيص ثمانية وعشرون شخصا من الذين يعانون من بخر الفم بالفحص السريري ) 11 ذكر و 11 أنثى( وتت ا روحأعمارهم بين ) 56 11 ( سنة. و جمعت تسع وخمسون عينة باستعمال الرؤوس الورقية المعقمة )حجم 65 ( أو مسحات قطنية -معقمة ثم نقمها في مرق thioglycolate وزرعت عمى وسط اكار الدم في ظروف هوائية أو لاهوائية لمدة ) 11 - 77 ( ساعة وتمتشخيص الج ا رثيم اعتمادا عمى الخصائص الشكمية والصفات الزرعية واختبا ا رت الحساسية لممضادات الحيوية. تم عزل وتشخيصثلاثة عشر نوعا من الج ا رثيم ) أربعة عشر عزلة من جنس Bacteriodes ، عشر عزلات من جنس Viridans Streptococci تسع عزلات من جنس Peptostreptococus ، ست عزلات من جنس Actinomyces ، اربع عزلات منجنس Porphymonos اربع عزلات من جنس Fusobacterium ، اربع عزلات من جنس Veillonella ، ثلاث عزلات منجنس Non-coagulase Staphylococcus . وعزلة من جنس Prevotella ، وعزلة من جنس Propinobacterium ، وعزلةمن جنس Tetragenococci ، وعزلة من جنس Eubacterium ، وعزلة من جنس Staphylococcus aureus

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