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Bacterial profiling of healthy bone marrow using polymerase chain reaction technique

Authors: Vidyut Prakash --- Deepak Kumar --- Saurabh Singh --- Gopal Nath
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2017 Volume: 6 Issue: 2 Pages: 69-73
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

BACKGROUND: The bone marrow of healthy individuals is conventionally considered sterile like otherbody fluids, but recently the scientists found genetic material from the bacteria inside their stem cells.The findings raise the possibility that other infectious agents may also reside in the bone marrow.OBJECTIVES: The study was planned to look for the presence of all type of bacteria by amplifying16S rDNA sequences using eubacterial universal primers.MATERIALS AND METHODS: A total of 18 bone marrow samples of apparently healthy individualswere collected from patients admitted having closed bone fractures. The DNA was isolated and wassubjected to nested polymerase chain reaction (PCR) using Universal eubacterial 16S rDNA primers.The samples positive by universal PCR was further checked for the presence of Salmonella Typhi,Salmonella Paratyphi A, and/or Mycobacterium tuberculosis (Mtb) if any using a second nestedPCR reaction.RESULTS: A total of 16 (89%) samples could yield the desired amplicon through universal PCR.The secondary PCR of 16 samples, the desired amplicons were detected 3 (18.8%) for Mtb, 4 (25%)for S. Typhi, and 1 (6.2%) for S. Paratyphi A.CONCLUSIONS: Even in asymptomatic cases, other infectious agents such as Mtb, Salmonellaspp., and many other microorganisms may be present as commensal.

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