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Use Polymerase Chain Reaction for Molecular Determination of Phenol
استخدام التفاعل متعدد السلسلة في لتشخيص الجزيئي للبكتريا المحطمة للفينول والمعزولة من مياه شط البصرة-العراق

Author: H.F. Mohammed هشام فياض محمد
Journal: Marsh Bulletin مجلة الاهوار ISSN: 18169848 Year: 2012 Volume: 7 Issue: 2 Pages: 162-168
Publisher: Basrah University جامعة البصرة

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Abstract

Phenol is one of the organic pollutants in various industrial wastewaters especially petrochemical and oil refining. Biological treatment is one of the considerable choices for removing of phenol from these wastewaters. Identification of effective microbial species is considered as one of the important priorities for bacteria productingbiomass in order to achieve desirable kinetic of biological reactions. Basic purpose of this research is identification of phenol-degrading Pseudomonasputidabypolymerase chain reaction (PCR) that has high speed and specificity . Amplification gene coding the N fragment in Pseudomonas putida-derived methyl phenol operon (DmpN gene) was used for specific identification of phenol-degrading Pseudomonas putida,According to the results of this study7 of 10 isolated bacteria Pseudomonas putidashowed a 199 bp PCR product by DmpNprimers.

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