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Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood

Authors: Hadeel H. Dawud --- Entesar H. Ali
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2018 Volume: 36 Issue: 2 Part (B) Engineering Pages: 152-155
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

The activity of urease purified from Proteus mirabilis bacteria wasestimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained asstandard in size (20- 40)nm, purity 99%. The enzyme activity was estimated byincubating the pure enzyme with various concentrations of nanoparticles rangedbetween (1- 6) μg/ml. The results indicates a decrease in enzymatic activity withincreasing of nanoparticles concentration. After that, the concentration of bloodurea was measured using urease obtained from a standard kit, urease purified fromProteus mirabilis and the urease- gamma iron nanoparticles solution. Thecomparison was then made among the results of urea concentrations by statisticalanalysis using T-test. The results showed that there is no significant differencebetween the results obtained from urease standard kit and urease purified fromthe bacteria, this is due to the efficiency of urease purified from the bacteria. On theother hand, the results showed that there is a significant difference (P≤ 0.01) inurea concentrations obtained from urease- gamma iron oxide nanoparticlessolution due to the inhibition of the enzyme which lost its activity by nanoparticles.

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